Salivary Metabolomics for Oral Precancerous Lesions: A Comprehensive Narrative Review

Oral submucous fibrosis (OSMF) is a chronic, potentially malignant disorder of the oral cavity, primarily associated with the consumption of areca nut products and other risk factors. Early and accurate diagnosis of OSMF is crucial to prevent its progression to oral cancer. In recent years, the field of metabolomics has gained momentum as a promising approach for disease detection and monitoring. Salivary metabolomics, a non-invasive and easily accessible diagnostic tool, has shown potential in identifying biomarkers associated with various oral diseases, including OSMF. This review synthesizes current literature on the application of salivary metabolomics in the context of OSMF detection. The review encompasses a comprehensive analysis of studies conducted over the past decade, highlighting advancements in analytical techniques, metabolomic profiling, and identified biomarkers linked to OSMF progression. The primary objective of this review is to provide a critical assessment of the feasibility and reliability of salivary metabolomics as a diagnostic tool for OSMF, along with its potential to differentiate OSMF from other oral disorders. In conclusion, salivary metabolomics holds great promise in revolutionizing OSMF detection through the identification of reliable biomarkers and the development of robust diagnostic models. However, challenges such as sample variability, validation of biomarkers, and standardization need to be addressed before its widespread clinical implementation. This review contributes to a comprehensive understanding of the current status, challenges, and future directions of salivary metabolomics in the realm of OSMF detection, emphasizing its potential impact on early intervention and improved patient outcomes

Household food insecurity and physical activity behaviour in Ecuadorian children and adolescents: findings from the Ecuador 2018 National Health and Nutrition Survey (ENSANUT-2018)

Abstract Objective: Ecuador has a high prevalence of household food insecurity (HFI) and is undergoing nutritional and epidemiologic transition. Evidence from high-income countries has reported negative or null associations between HFI and physical activity (PA) in children. It remains uncertain whether the same is true of those from low- and middle-income countries like Ecuador whose environmental and socio-demographic characteristics are distinct from those of high-income countries. We aimed to investigate the association of HFI with PA, sedentary behaviour (SB) and anthropometric indicators in children. Design: Cross-sectional analysis of data from the nationally representative 2018 Ecuadorian National Health and Nutrition Survey. Data were collected on HFI, PA, SB, socio-demographic characteristics and measured height and weight. Unadjusted and adjusted linear, log-binomial and multinomial logistic regression analyses assessed the relationship of HFI with PA, SB, stunting and BMI-for-age. Setting: Ecuador. Participants: 23 621 children aged 5–17 years. Results: Marginal and moderate-severe HFI was prevalent in 24 % and 20 % of the households, respectively. HFI was not associated with PA, SB, stunting nor underweight. Moderate-severe HFI was associated with a lower odds of overweight and obesity. However, adjustment for household assets attenuated this finding for overweight (adjusted OR:0·90, 95 % CI: 0·77, 1·05) and obesity (adjusted OR: 0·88, 95 % CI: 0·71, 1·08). Conclusion: HFI is a burden in Ecuadorian households, but is not associated with PA, SB nor anthropometric indicators in children aged 5–17 years. However, a concerning prevalence of insufficient PA was reported, emphasising the critical need for evidence-based interventions aimed at promoting PA and reducing SB

Health effects of arsenic exposure in Latin America: An overview of the past eight years of research

Studies conducted over the past eight years in Latin America (LA) have continued to produce new knowledge regarding health impacts of arsenic (As) in drinking water. We conducted a systematic review of 92 peer-reviewed English articles published between 2011 and 2018 to expand our understanding on these health effects. Majority of the LA studies on As have been conducted in Chile and Mexico. Additional data have emerged from As-exposed populations in Argentina, Bolivia, Brazil, Colombia, Ecuador, and Uruguay. The present review has documented recent data on the biomarkers of As exposure, genetic susceptibility and genotoxicity, and risk assessment to further characterize the health effects and exposed populations. Some recent findings on the associations of As with bladder and lung cancers, reproductive outcomes, and declined cognitive performance have been consistent with what we reported in our previous systematic review article. We have found highly convincing evidence of in utero As exposure as a significant risk factor for several health outcomes, particularly for bladder cancer, even at moderate level. New data have emerged regarding the associations of As with breast and laryngeal cancers as well as type 2 diabetes. We observed early life As exposure to be associated with kidney injury, carotid intima-media thickness, and various pulmonary outcomes in children. Other childhood effects such as low birth weight, low gestational age, anemia, increased apoptosis, and decreased cognitive functions were also reported. Studies identified genetic variants of As methyltransferase that could determine susceptibility to As related health outcomes. Arsenic-induced DNA damage and alteration of gene and protein expression have also been reported. While the scope of research is still vast, the substantial work done on As exposure and its health effects in LA will help direct further large-scale studies for more comprehensive knowledge and plan appropriate mitigation strategies

Antibiotic Resistance in Microbes from Street Fruit Drinks and Hygiene Behavior of the Vendors in Delhi, India

Microbial contamination of fruit juices has caused major outbreaks, leading to significant morbidity and mortality in developing countries. The inept hygiene and safety practices followed by the juice vendors are the leading risk factors of the microbial contamination of juices. In this pilot study, the five most crowded markets in urban Delhi, including Kamla Nagar, University of Delhi (north campus), Tilak Nagar, Chandni Chowk, and Rohini, were selected for a questionnaire survey on the fruit juice vendors and the sampling of water used for juice preparation as well as sugarcane, orange, and mix fruit juices collected from these markets for the enumeration of total bacterial count (TBC), Escherichia coli, Salmonella, and Vibrio. Antibiotic susceptibility tests were performed using ampicillin, cefotaxime, chloramphenicol, ciprofloxacin, and imipenem. The results indicated that the majority of the vendors were not following hygiene and safety practices when compared with the recommended standard safety practices. The use of municipal water by 95% of vendors with high TBC counts might have been the major source of microbial contamination in all types of fruit juices. E. coli and Salmonella contaminations were high in sugarcane (2 × 105 colony forming units (CFU)/mL) and mix fruit (2.2 × 105 CFU/mL) juice samples, respectively. On the other hand, Vibrio was found to be absent in almost all juice samples except for orange juice. All strains were found to be susceptible to chloramphenicol, but resistant to ampicillin and cefotaxime. Only a few strains were resistant to ciprofloxacin, and only E. coli strains were resistant to imipenem. Taken together, the overall microbiological standards of fruit juices served by street vendors were not within the acceptable limits, perhaps due to the poor quality of water used to prepare juices and poor hygiene and safety practices followed by the vendors. More importantly, the isolated microbes demonstrated resistance to ampicillin and cefotaxime, which may have pressing public health implications. Post hoc power analyses identified the minimum sample size required for 80% power

Estimating the association between parity and mid- to later-life cognitive function in rural South Africa: Evidence from “Health and Aging in Africa: A Longitudinal Study of an INDEPTH Community in South Africa” (HAALSI)

BackgroundThe burden of cognitive decline is projected to rise over the next decades, particularly in low- and middle-income countries, contributing to economic hardship, morbidity, and mortality. Reproductive life-history, including parity, may be associated with later-life cognitive ability. Using data from the population-representative “Health and Aging in Africa: A Longitudinal Study of an INDEPTH Community in South Africa” (HAALSI) cohort, we estimated the association between parity and mid- to later-life global cognitive function.MethodData were from in-person interviews with 2242 men and 2604 women aged ≥40 in the rural Agincourt sub-district, Mpumalanga province, South Africa in 2014/15. Cognitive function was assessed as time orientation and immediate and delayed recall of a 10-word list. Number of living children was self-reported. We used multivariable linear regression with parity (number of children: 0, 1-2, 3-4, 5-9, 10+) as the categorical predictor and z-standardized cognitive function as the outcome. Analyses were stratified by sex/gender, controlling for age, education, and literacy.ResultAmong men, there was a U-shaped relationship between parity and cognitive function (p<0.001), whereby men with 3-4 children had the highest mean z-standardized cognitive scores (mean: 0.244, SD: 0.984, Table 1, Figure 1). Women also had a U-shaped relationship between parity and cognitive function (p = 0.0254), whereby women with 5-9 children had the highest mean z-standardized cognitive scores (mean: -0.017, SD: 0.935, Table 2, Figure 1). Among men, adjusted regression models showed that parity was associated with higher cognitive function, across all categories compared to no children (Estimates [95% CI]: 1-2: 0.32 [0.17-0.48], 3-4: 0.50 [0.35-0.65], 5-9: 0.50 [0.36-0.74], 10+: 0.55 [0.36-0.74]; Table 3). Among women, only the 5-9 children group (Estimate [95% CI]: 0.16 [0.00-0.31]) had cognitive scores that were significantly different from having no children (Table 3).ConclusionData from this representative sample in rural South Africa indicate that the relationship between parity and later life cognition varies by sex/gender, with having children being associated with greater cognitive performance in men, compared to women. This sex/gender difference may be due to sex and gender differences in biological and social roles involved in childbearing and rearing.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/175431/1/alz062318.pd

Health Implications of Drinking Water Salinity in Coastal Areas of Bangladesh

Coastal areas in South Asian countries are particularly vulnerable to elevated water salinity. Drinking water salinity has been found to be associated with cardiovascular diseases (CVD), diarrhea, and abdominal pain. Our study aimed to find if excess drinking water salinity was associated with increased hospital visits with an array of health effects in coastal sub-districts of Bangladesh. A cross-sectional study was conducted with 157 participants from three coastal sub-districts. A face-to-face interview was conducted to collect salinity exposure and hospital visit data. Water samples were collected from both drinking and other household water sources for the measurement of salinity and total dissolved solids (TDS). CVD, diarrhea, and abdominal pain related hospital visits were found to be significantly associated with high water salinity and TDS. Households exposed to high salinity demonstrated a higher frequency of hospital visits than the low salinity-exposed households. People exposed to high salinity seemed to lack awareness regarding salinity-inducing health effects. Water salinity is a public health concern that will continue to rise due to climate change. Therefore, raising awareness about the health risks of water salinity is essential for the government to frame policies and mitigation strategies to control this emerging threat

Association between Handwashing Behavior and Infectious Diseases among Low-Income Community Children in Urban New Delhi, India: A Cross-Sectional Study

Diarrheal diseases and respiratory infections (RI) are two leading causes of childhood mortality in low and middle-income countries. Effective handwashing at critical time-points may mitigate these diseases. However, there is a lack of published data investigating this association in school-aged children in India. This study is part of a larger prospective handwashing intervention study in a low-income community in New Delhi, India examining the associations between handwashing behavior and diarrhea and RI in schoolchildren. This current study reports the findings of the baseline survey administered to 272 mother&ndash;child dyads. Children aged 8&ndash;12 years, and their mothers, were recruited from six schools. A baseline questionnaire was used to collect sociodemographic data, handwash behavior, and mother-reported recent diarrhea and RI incidence among the children. Handwashing before and after preparing food, after defecation, and after cleaning dishes significantly reduced the odds of diarrhea by over 70%, and of RI by over 56%. Using a clean cloth after handwashing lowered odds of diarrhea and RI by 72% and 63% respectively. Around 60% of the participants believed that handwashing could prevent diarrhea and RI in their children. There was a low prevalence of handwashing at critical time-points and a poor perception regarding handwashing benefits. To improve handwashing behavior, hygiene promotion programs need to understand what motivates and hinders handwashing in vulnerable populations

Child support grant expansion and cognitive function among women in rural South Africa : findings from a natural experiment in the HAALSI cohort

Background: Cash transfers are a promising but understudied intervention that may protect cognitive function in adults. Although South Africa has a rapidly ageing population, little is known about the nature of association between cash transfers and cognitive function in this setting. Objectives We leveraged age-eligibility expansions to South Africa's Child Support Grant (CSG) to investigate the association between duration of CSG eligibility and cognitive function of biological mothers of child beneficiaries in South Africa. Methods: We analysed 2014/2015 baseline data from 944 women, aged 40–59 years with at least one CSG-eligible child, enrolled in the population-representative HAALSI cohort in Agincourt, South Africa. Duration of CSG eligibility for each mother was calculated based on the birth dates of all their children and the CSG age-eligibility expansion years (2003–2012). Cognitive function was measured using a cognitive battery administered at the HAALSI baseline interview. Linear regression was used to estimate the association between duration of CSG eligibility, dichotomized as low (≤10 years) and high (&gt;10 years) eligibility, and cognitive function z-scores of the mothers. Results: High vs. low duration of CSG eligibility, was associated with higher cognitive function z-scores in the full sample [β: 0.15 SD units; 95% CI: 0.04, 0.26; p-value = 0.01]. In mothers with one to four lifetime children, but not five or more, high vs. low duration of CSG eligibility, was associated with higher cognitive function z-scores [β: 0.19 SD units; 95% CI: 0.05, 0.34, p-value = 0.02]. Conclusion: Government cash transfers given to support raising children may confer substantial protective effects on the subsequent cognitive function of mothers. Further studies are needed to understand how parity may influence this relationship. Our findings bring evidence to policymakers for designing income supplementation programmes to promote healthy cognitive ageing in low-income settings

Estimated effects of high child support grant eligibility (>10 years) compared to low child support grant eligibility (≤10 years) on cognitive function in child support grant-eligible mothers, health and ageing in africa: A longitudinal study of an INDEPTH community in South Africa, 2014/15.

Estimated effects of high child support grant eligibility (>10 years) compared to low child support grant eligibility (≤10 years) on cognitive function in child support grant-eligible mothers, health and ageing in africa: A longitudinal study of an INDEPTH community in South Africa, 2014/15.</p