Ultraestructura de la red alveolar y su relación con el recubrimiento de las paredes vasculares en olmos infectados con Ophiostoma novo-ulmi y en otras plantas infectadas con enfermedades similares de marchitamiento

In elms infected with Dutch elm disease, alveolar networks, demarcated by filamentous-like bands and confluent with similar matter (the coating) accumulating on vessel walls, occurred regularly in vessel elements. Similar material lined vessel walls in inoculated, sterilized, thin elm wood sections fixed by high pressure freezing. The coating was observed to connect with fungal cells and occasionally contained small opaque particles, the size of ribosomes, membranous and vesicular structures, and, following incubation of wood chips taken from diseased samples incubated on an agar medium, it still displayed similar matter. Coating and alveolar bands increased in thickness by confluence of other bands or membranous structures. Similar matter and structures also occurred in other plants affected by similar fungal wilt diseases. In all systems, the compact coating did not label for chitin, cellulose and pectin. In staghorn sumac, the probe for DNA attached to the coating. Altogether, in the light of these data, it appears that the coating and alveolar networks are not inert components, a fact which indicates their primordial probable pathogen origin. It is proposed that these elements might be important not only in the initial infection stages but also in older or recurrent infections at a time when host resistance mechanisms are ineffective.En olmos afectados por la grafiosis, la red alveolar, demarcada por bandas filamentosas, y confluente con acumulaciones de la misma sustancia (cubrición) presentes en las paredes de los vasos, aparece regularmente en los elementos conductores. Sustancias similares tapizan las paredes de los vasos en secciones de madera delgada de olmo inoculada y esterilizada, y posteriormente criofijadas a altas presiones. Se observó que la cubrición se conecta con las células del micelio y que ocasionalmente contenía pequeñas partículas opacas del tamaño de los ribosomas, estructuras membranosas y vesiculares, así como que, tras la incubación de astillas leñosas cogidas de muestras enfermas incubadas en agar, aún se presentaba una sustancia similar. La cubrición y las bandas alveolares aumentaron su espesor en la confluencia con otras bandas o estructuras membranosas. Estructuras y sustancias similares aparecieron también en otras plantas afectadas por enfermedades similares originadas por hongos que producen marchitamiento. En todos los sistemas, la cubrición compacta no pudo ser marcada como quitina, celulosa ni pectina. En zumaque (Rhus typhina), la sonda de ADN se pegó a la cubrición. En resumen, a la vista de estos datos, parece ser que la cubrición y la red alveolar no están formados por componentes inertes, un hecho que indica su probable origen patogénico. Se sugiere que esos elementos podrían ser importantes no sólo en las fases iniciales de la infección, sino también, en infecciones más desarrolladas o recurrentes, en el momento en que los mecanismos de resistencia del hospedante no son efectivos

2016 Decadal Update of the NASA ESTO Lidar Technologies Investment Strategy

We describe the 2016 update of the NASA Earth Science Technology Office (ESTO) investment strategy in the area of lidar technologies as pertaining to NASAs Earth Science measurement goals in the next decade

Wide-Field InfraRed Survey Telescope (WFIRST) Final Report

In December 2010, NASA created a Science Definition Team (SDT) for WFIRST, the Wide Field Infra-Red Survey Telescope, recommended by the Astro 2010 Decadal Survey as the highest priority for a large space mission. The SDT was chartered to work with the WFIRST Project Office at GSFC and the Program Office at JPL to produce a Design Reference Mission (DRM) for WFIRST. Part of the original charge was to produce an interim design reference mission by mid-2011. That document was delivered to NASA and widely circulated within the astronomical community. In late 2011 the Astrophysics Division augmented its original charge, asking for two design reference missions. The first of these, DRM1, was to be a finalized version of the interim DRM, reducing overall mission costs where possible. The second of these, DRM2, was to identify and eliminate capabilities that overlapped with those of NASA's James Webb Space Telescope (henceforth JWST), ESA's Euclid mission, and the NSF's ground-based Large Synoptic Survey Telescope (henceforth LSST), and again to reduce overall mission cost, while staying faithful to NWNH. This report presents both DRM1 and DRM2.Comment: 102 pages, 57 figures, 17 table

First Astronomical Use of Multiplexed Transition Edge Bolometers

We present performance results based on the first astronomical use of multiplexed superconducting bolometers. The Fabry-Perot Interferometer Bolometer Research Experiment (FIBRE) is a broadband submillimeter spectrometer that achieved first light in June 2001 at the Caltech Submillimeter Observatory (CSO). FIBRE'S detectors are superconducting transition edge sensor (TES) bolometers read out by a SQUID multiplexer. The Fabry-Perot uses a low resolution grating to order sort the incoming light. A linear bolometer array consisting of 16 elements detects this dispersed light, capturing 5 orders simultaneously from one position on the sky. With tuning of the Fabry-Perot over one free spectral range, a spectrum covering Δλ/λ= 1/7 at a resolution of δλ/λ ≈ 1/1200 can be acquired. This spectral resolution is sufficient to resolve Doppler-broadened line emission from external galaxies. FIBRE operates in the 350 µm and 450 µm bands. These bands cover line emission from the important star formation tracers neutral carbon [Cl] and carbon monoxide (CO). We have verified that the multiplexed bolometers are photon noise limited even with the low power present in moderate resolution spectrometry

Genetic, serological and biochemical characterization of Leishmania tropica from foci in northern Palestine and discovery of zymodeme MON-307

Background Many cases of cutaneous leishmaniasis (CL) have been recorded in the Jenin District based on their clinical appearance. Here, their parasites have been characterized in depth. Methods Leishmanial parasites isolated from 12 human cases of CL from the Jenin District were cultured as promastigotes, whose DNA was extracted. The ITS1 sequence and the 7SL RNA gene were analysed as was the kinetoplast minicircle DNA (kDNA) sequence. Excreted factor (EF) serotyping and multilocus enzyme electrophoresis (MLEE) were also applied. Results This extensive characterization identified the strains as Leishmania tropica of two very distinct sub-types that parallel the two sub-groups discerned by multilocus microsatellite typing (MLMT) done previously. A high degree of congruity was displayed among the results generated by the different analytical methods that had examined various cellular components and exposed intra-specific heterogeneity among the 12 strains. Three of the ten strains subjected to MLEE constituted a new zymodeme, zymodeme MON-307, and seven belonged to the known zymodeme MON-137. Ten of the 15 enzymes in the profile of zymodeme MON-307 displayed different electrophoretic mobilities compared with the enzyme profile of the zymodeme MON-137. The closest profile to that of zymodeme MON-307 was that of the zymodeme MON-76 known from Syria. Strains of the zymodeme MON-307 were EF sub-serotype A2 and those of the zymodeme MON-137 were either A9 or A9B4. The sub-serotype B4 component appears, so far, to be unique to some strains of L. tropica of zymodeme MON-137. Strains of the zymodeme MON-137 displayed a distinctive fragment of 417 bp that was absent in those of zymodeme MON-307 when their kDNA was digested with the endonuclease RsaI. kDNA-RFLP after digestion with the endonuclease MboI facilitated a further level of differentiation that partially coincided with the geographical distribution of the human cases from which the strains came. Conclusions The Palestinian strains that were assigned to different genetic groups differed in their MLEE profiles and their EF types. A new zymodeme, zymodeme MON-307 was discovered that seems to be unique to the northern part of the Palestinian West Bank. What seemed to be a straight forward classical situation of L. tropica causing anthroponotic CL in the Jenin District might be a more complex situation, owing to the presence of two separate sub-types of L. tropica that, possibly, indicates two separate transmission cycles involving two separate types of phlebotomine sand fly vector

Ulcerative colitis-risk loci on chromosomes 1p36 and 12q15 found by genome-wide association study.

Ulcerative colitis is a chronic inflammatory disease of the colon that presents as diarrhea and gastrointestinal bleeding. We performed a genome-wide association study using DNA samples from 1,052 individuals with ulcerative colitis and preexisting data from 2,571 controls, all of European ancestry. In an analysis that controlled for gender and population structure, ulcerative colitis loci attaining genome-wide significance and subsequent replication in two independent populations were identified on chromosomes 1p36 (rs6426833, combined P = 5.1 x 10(-13), combined odds ratio OR = 0.73) and 12q15 (rs1558744, combined P = 2.5 x 10(-12), combined OR = 1.35). In addition, combined genome-wide significant evidence for association was found in a region spanning BTNL2 to HLA-DQB1 on chromosome 6p21 (rs2395185, combined P = 1.0 x 10(-16), combined OR = 0.66) and at the IL23R locus on chromosome 1p31 (rs11209026, combined P = 1.3 x 10(-8), combined OR = 0.56; rs10889677, combined P = 1.3 x 10(-8), combined OR = 1.29)

Epidemiologic Relationship between Toscana Virus Infection and Leishmania infantum Due to Common Exposure to Phlebotomus perniciosus Sandfly Vector

Sand flies are recognised vectors of parasites in the genus Leishmania and a number of arthropod-borne viruses, in particular viruses within the genus Phlebovirus, family Bunyaviridae. In southern France, Toscana phlebovirus (TOSV) is recognized as a prominent cause of summer meningitis. Since Leishmania and TOSV have a common vector (Phlebotomus perniciosus), an epidemiologic link has been assumed for a long time. However, there is no scientific evidence of such a link between human leishmaniosis and phleboviral infections. To identify a possible link, we investigated the presence and distribution of antibodies against these two microorganisms (i) in individuals and (ii) at a spatial level in the city of Marseille (south-eastern France). Five hundred sera were selected randomly in the biobank of the Department of Parasitology of the Public Hospitals of Marseille. All sera were previously tested for IgG against Leishmania by Western Blotting, and TOSV IgG were detected by indirect immunofluorescence. The seropositivity rates were 21.4% for TOSV and 28% for Leishmania. Statistical analysis demonstrated that seropositivity for one pathogen was significantly associated with seropositivity to the other pathogen. This result provided the first robust evidence for the existence of an epidemiological relationship between Leishmania infantum and TOSV. Addresses of tested patients were geolocalized and integrated into Geographical Information System software, in order to test spatial relationship between the two pathogens. Spatial analysis did not allow to identify (i) specific patterns for the spatial distribution of positive serological results for TOSV or Leishmania, and (ii) a spatial relationship between Leishmania and TOSV positive serological results. This may reflect the fact that the sample studied was not powerful enough to demonstrate either a spatial clustering or co-location, i.e. that the actual risk exposure area is smaller than the mean of distance between patients in our study (245 m)

Wide-Field Infrared Survey Telescope (WFIRST) Interim Report

The New Worlds, New Horizons (NWNH) in Astronomy and Astrophysics 2010 Decadal Survey prioritized the community consensus for ground-based and space-based observatories. Recognizing that many of the community s key questions could be answered with a wide-field infrared survey telescope in space, and that the decade would be one of budget austerity, WFIRST was top ranked in the large space mission category. In addition to the powerful new science that could be accomplished with a wide-field infrared telescope, the WFIRST mission was determined to be both technologically ready and only a small fraction of the cost of previous flagship missions, such as HST or JWST. In response to the top ranking by the community, NASA formed the WFIRST Science Definition Team (SDT) and Project Office. The SDT was charged with fleshing out the NWNH scientific requirements to a greater level of detail. NWNH evaluated the risk and cost of the JDEM-Omega mission design, as submitted by NASA, and stated that it should serve as the basis for the WFIRST mission. The SDT and Project Office were charged with developing a mission optimized for achieving the science goals laid out by the NWNH re-port. The SDT and Project Office opted to use the JDEM-Omega hardware configuration as an initial start-ing point for the hardware implementation. JDEM-Omega and WFIRST both have an infrared imager with a filter wheel, as well as counter-dispersed moderate resolution spectrometers. The primary advantage of space observations is being above the Earth's atmosphere, which absorbs, scatters, warps and emits light. Observing from above the atmosphere enables WFIRST to obtain precision infrared measurements of the shapes of galaxies for weak lensing, infrared light-curves of supernovae and exoplanet microlensing events with low systematic errors, and infrared measurements of the H hydrogen line to be cleanly detected in the 1<z<2 redshift range important for baryon acoustic oscillation (BAO) dark energy measurements. The Infrared Astronomical Satellite (IRAS), the Cosmic Background Explorer (COBE), Herschel, Spitzer, and Wide-field Infrared Sur-vey Explorer (WISE) are all space missions that have produced stunning new scientific advances by going to space to observe in the infrared. This interim report describes progress as of June 2011 on developing a requirements flowdown and an evaluation of scientific performance. An Interim Design Reference Mission (IDRM) configuration is presented that is based on the specifications of NWNH with some refinements to optimize the design in accordance with the new scientific requirements. Analysis of this WFIRST IDRM concept is in progress to ensure the capability of the observatory is compatible with the science requirements. The SDT and Project will continue to refine the mission concept over the coming year as design, analysis and simulation work are completed, resulting in the SDT s WFIRST Design Reference Mission (DRM) by the end of 2012