Mono- and Dinuclear Complexes of a New Binucleating Porphyrin, α,α-5,15-Bis(ο-(Nicotinoylamino)Phenyl)-2,8,12,18-Tetraethyl-3,7,13,17-Tetramethylporphyrin. Crystal Structures of a Mononuclear Nickel(II) Complex and a Binuclear Copper-Platinum Complex

The synthesis and characterization of a new binucleating porphyrin ligand, a,a-5, 1 5-bis(o-(nicotinoylamido)phenyl)-2,8, 12,18- tetraethyl-3,7,13,17-tetramethylporphyrin, [H2(DPE)]-(pyh is reported. Treatment of [H 2(DPE)]-(pyh with methanol solutions of Ni(II) or Cu(II) leads to the formation of mononuclear metal complexes, [M(DPE)]-(pyh, in which the metal has inserted into the porphyrin core, leaving the pyridine binding site free. Addition of a second metal to form a binuclear complex, [M( DPE)]-(pyhM\u27Cl2, can be accomplished using the reagent M\u27(DMSOhCI2, M\u27 = Pd, Pt, and Zn. [Ni(DPE)]-(pyh and [Cu(DPE)]-(pyhPtCI2 have been characterized by single-crystal X-ray analysis. Crystal data for [Ni(DPE)]-(pyh: Ni02N 8- C56H52·2CHCI3, triclinic, PI, a= 14.306 (4) A, b = 14.719 (5) A, c = 14.296 (5) A, a= 94.86 (3) 0 , {J = 96.38 (3) 0 , -y = 63.45 (2) 0 , Z = 2, R = 0.049, and Rw = 0.064. Crystal data for [Cu(DPE)]-(pyhPtCI2: CuPt02N 8C 56H 52, triclinic, PI, a= 19.333 (1) A, b = 23.74 (I) A, c = 12.984 (5) A, a= 103.28 (4) 0 , fJ = 108.29 (3) 0 , -r = 76.47 (3) 0 , Z = 4, R = 0.073, and Rw = 0.085. Pt-Pt distances between the two molecules in the asymmetric unit are 3.766 (3) A

Digital Mediation, Connectivity and Affective Materialities

Physical cultural studies (PCS) is a dynamic and rapidly developing field of study. This handbook offers the first definitive account of the state of the art in PCS, showcasing the latest research and methodological approaches

Soil Phosphate Solubilising Bacteria of the Okavango Floodplains, Botswana

Phosphorus although abundant is among the most limiting macro plant nutrients in some soils. Deficiencies are often seen as purpling of leaves due to unavailability of PO4 in soil solution. In soil solution PO4 solubility is pH dependent; at pH \u3c 6, it is often bound to Fe or Al, while at pH \u3e 7 it forms insoluble complexes with Ca, Mg, K or Na making it unavailable to plants (Brady 2002). Soil organic P requires mineralisation then solubilisation by the enzyme phosphatase before being available. Botswana soils due to the low rainfall (\u3c 450 mm/yr) are characterised by high pH and salt pans scattered all over the semi-arid and arid landscape. Cultivation of most of these soils often results in P deficient crops (Leuschner and Manthe 1996). However, in grassland areas of the Okavango, very few grasses show P deficiency. Studies from other areas indicate that some grasses habour PO4 solubilizing bacteria in their rhizosphere that help them in their P sequencing quest (Duponnois et al. 2005). This study was set up to study the PO4 solubilising bacteria of the Okavango Delta at Seronga. The study also looked at possible mechanisms of solubilisation employed by the bacteria. In the long run, the study will also attempt to use these bacteria on locally grown staple cereal, sorghum (Sorghum vulgare)

Pornography, sexual coercion and abuse and sexting in young people’s intimate relationships: A European study

New technology has made pornography increasingly accessible to young people, and a growing evidence base has identified a relationship between viewing pornography and violent or abusive behavior in young men. This article reports findings from a large survey of 4,564 young people aged 14 to 17 in five European countries which illuminate the relationship between regular viewing of online pornography, sexual coercion and abuse and the sending and receiving of sexual images and messages, known as “sexting.” In addition to the survey, which was completed in schools, 91 interviews were undertaken with young people who had direct experience of interpersonal violence and abuse in their own relationships. Rates for regularly viewing online pornography were very much higher among boys and most had chosen to watch pornography. Boys’ perpetration of sexual coercion and abuse was significantly associated with regular viewing of online pornography. Viewing online pornography was also associated with a significantly increased probability of having sent sexual images/messages for boys in nearly all countries. In addition, boys who regularly watched online pornography were significantly more likely to hold negative gender attitudes. The qualitative interviews illustrated that, although sexting is normalized and perceived positively by most young people, it has the potential to reproduce sexist features of pornography such as control and humiliation. Sex and relationships education should aim to promote a critical understanding of pornography among young people that recognizes its abusive and gendered values

AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells

Chronic myeloid leukemia (CML) represents the first human malignancy successfully treated with a tyrosine kinase inhibitor (TKI; imatinib). However, early relapses and the emergence of imatinib-resistant disease are problematic. Evidence suggests that imatinib and other inhibitors may not effectively eradicate leukemic stem/progenitor cells, and that combination therapy directed to complimentary targets may improve treatment. Abelson helper integration site 1 (Ahi-1)/AHI-1 is a novel oncogene that is highly deregulated in CML stem/progenitor cells where levels of BCR-ABL transcripts are also elevated. Here, we demonstrate that overexpression of Ahi-1/AHI-1 in murine and human hematopoietic cells confer growth advantages in vitro and induce leukemia in vivo, enhancing effects of BCR-ABL. Conversely, RNAi-mediated suppression of AHI-1 in BCR-ABL–transduced lin−CD34+ human cord blood cells and primary CML stem/progenitor cells reduces their growth autonomy in vitro. Interestingly, coexpression of Ahi-1 in BCR-ABL–inducible cells reverses growth deficiencies exhibited by BCR-ABL down-regulation and is associated with sustained phosphorylation of BCR-ABL and enhanced activation of JAK2–STAT5. Moreover, we identified an AHI-1–BCR-ABL–JAK2 interaction complex and found that modulation of AHI-1 expression regulates phosphorylation of BCR-ABL and JAK2–STAT5 in CML cells. Importantly, this complex mediates TKI response/resistance of CML stem/progenitor cells. These studies implicate AHI-1 as a potential therapeutic target downstream of BCR-ABL in CML