Mathematically formulated key performance indicators for design and evaluation of treatment trains for resource recovery from urban wastewater

While urban wastewater infrastructure is aging and no longer adequate, climate change and sustainability are urging the transition from pollution management to resource recovery. Lacking evidence-based quantitative evaluation of the potential benefits and consequences of resource recovery from wastewater hinders the negotiation amongst stakeholders and slows down the transition. This study proposes mathematical formulations for technical, environmental, economic, and social key performance indicators (KPIs) that can be used to quantify the benefits and the risks of resource recovery. The proposed formulations are derived from the literature and validated with stakeholders. Each KPI is mathematically formulated at treatment train level by considering: (1) the characteristics of individual unit processes (UPs) in the treatment train (TT), (2) the context in which the TT is installed, and (3) the resources to be recovered. The mathematical formulations of the KPIs proposed in this study enable a transparent, consistent and informative evaluation of existing treatment trains, as well as support the (computer aided) design of new ones. This could aid the transition from urban wastewater treatment to resource recovery from urban wastewater.</p

Aerobic nonylphenol degradation and nitro-nonylphenol formation by microbial cultures from sediments

Nonylphenol (NP) is an estrogenic pollutant which is widely present in the aquatic environment. Biodegradation of NP can reduce the toxicological risk. In this study, aerobic biodegradation of NP in river sediment was investigated. The sediment used for the microcosm experiments was aged polluted with NP. The biodegradation of NP in the sediment occurred within 8 days with a lag phase of 2 days at 30°C. During the biodegradation, nitro-nonylphenol metabolites were formed, which were further degraded to unknown compounds. The attached nitro-group originated from the ammonium in the medium. Five subsequent transfers were performed from original sediment and yielded a final stable population. In this NP-degrading culture, the microorganisms possibly involved in the biotransformation of NP to nitro-nonylphenol were related to ammonium-oxidizing bacteria. Besides the degradation of NP via nitro-nonylphenol, bacteria related to phenol-degrading species, which degrade phenol via ring cleavage, are abundantly present

Influence of Substrates on the Surface Characteristics and Membrane Proteome of Fibrobacter succinogenes S85

Although Fibrobacter succinogenes S85 is one of the most proficient cellulose degrading bacteria among all mesophilic organisms in the rumen of herbivores, the molecular mechanism behind cellulose degradation by this bacterium is not fully elucidated. Previous studies have indicated that cell surface proteins might play a role in adhesion to and subsequent degradation of cellulose in this bacterium. It has also been suggested that cellulose degradation machinery on the surface may be selectively expressed in response to the presence of cellulose. Based on the genome sequence, several models of cellulose degradation have been suggested. The aim of this study is to evaluate the role of the cell envelope proteins in adhesion to cellulose and to gain a better understanding of the subsequent cellulose degradation mechanism in this bacterium. Comparative analysis of the surface (exposed outer membrane) chemistry of the cells grown in glucose, acid-swollen cellulose and microcrystalline cellulose using physico-chemical characterisation techniques such as electrophoretic mobility analysis, microbial adhesion to hydrocarbons assay and Fourier transform infra-red spectroscopy, suggest that adhesion to cellulose is a consequence of an increase in protein display and a concomitant reduction in the cell surface polysaccharides in the presence of cellulose. In order to gain further understanding of the molecular mechanism of cellulose degradation in this bacterium, the cell envelope-associated proteins were enriched using affinity purification and identified by tandem mass spectrometry. In total, 185 cell envelope-associated proteins were confidently identified. Of these, 25 proteins are predicted to be involved in cellulose adhesion and degradation, and 43 proteins are involved in solute transport and energy generation. Our results supports the model that cellulose degradation in F. succinogenes occurs at the outer membrane with active transport of cellodextrins across for further metabolism of cellodextrins to glucose in the periplasmic space and inner cytoplasmic membrane

Current trend in synthesis, Post-Synthetic modifications and biological applications of Nanometal-Organic frameworks (NMOFs)

Since the early reports of MOFs and their interesting properties, research involving these materials has grown wide in scope and applications. Various synthetic approaches have ensued in view of obtaining materials with optimised properties, the extensive scope of application spanning from energy, gas sorption, catalysis biological applications has meant exponentially evolved over the years. The far‐reaching synthetic and PSM approaches and porosity control possibilities have continued to serve as a motivation for research on these materials. With respect to the biological applications, MOFs have shown promise as good candidates in applications involving drug delivery, BioMOFs, sensing, imaging amongst others. Despite being a while away from successful entry into the market, observed results in sensing, drug delivery, and imaging put these materials on the spot light as candidates poised to usher in a revolution in biology. In this regard, this review article focuses current approaches in synthesis, post functionalization and biological applications of these materials with particular attention on drug delivery, imaging, sensing and BioMOFs

Homogeneous photosensitized degradation of pharmaceuticals by using red light LED as light source and methylene blue as photosensitizer

Research on employing advanced oxidation processes (AOPs) for pharmaceuticals removal is gaining interests. However, detrimental effects of background water constituents in complex water matrices are limiting their implementation. In this study, we report red light induced methylene blue photosensitization (MB/Red-light) as a promising alternative for pharmaceuticals removal from wastewater, because of its potential to overcome detrimental effects of background water constituents as experienced in other AOP technologies. In this study, the efficacy of MB/Red-light on degradation of four pharmaceutical compounds, i.e. diclofenac (DFN), metoprolol (MTP), propranolol (PRP) and sulfamethoxazole (SFZ), was investigated. The MB/Red-light photosensitization enabled degradation of three model compounds, i.e. DFN, PRP and SFZ. Degradation rates followed the order of DFN ＞ PRP ＞ SFZ. Singlet oxygen was found to be crucial in pharmaceuticals degradation, and another additional mechanism, i.e. a direct reaction with triplet MB, also contributed to DFN degradation. The presence of two DFN degradation mechanisms were confirmed by UV–vis light absorbance spectra measurement as well as the identification of degradation products by LC–MS/MS. Effects of three common back ground water constituents were examined to assess the applicability of MB/red-light system in complex water matrices, which suggests that the MB/red-light system has great potential to be used in real wastewater. Higher pH was found to impose positive impact on the efficacy of the proposed system. Last but not least, red light LED is an optimum light source for the proposed MB/Red-light system, because the light emission spectrum of the LED used in this study fits well with the light absorption spectrum of the photosensitizer-methylene blue

On the sensitivity of local flexibility markets to forecast error: A bi-level optimization approach

The large-scale integration of intermittent distributed energy resources has led to increased uncertainty in the planning and operation of distribution networks. The optimal flexibility dispatch is a recently introduced, power flow-based method that a distribution system operator can use to effectively determine the amount of flexibility it needs to procure from the controllable resources available on the demand side. However, the drawback of this method is that the optimal flexibility dispatch is inexact due to the relaxation error inherent in the second-order cone formulation. In this paper we propose a novel bi-level optimization problem, where the upper level problem seeks to minimize the relaxation error and the lower level solves the earlier introduced convex second-order cone optimal flexibility dispatch (SOC-OFD) problem. To make the problem tractable, we introduce an innovative reformulation to recast the bi-level problem as a non-linear, single level optimization problem which results in no loss of accuracy. We subsequently investigate the sensitivity of the optimal flexibility schedules and the locational flexibility prices with respect to uncertainty in load forecast and flexibility ranges of the demand response providers which are input parameters to the problem. The sensitivity analysis is performed based on the perturbed Karush-Kuhn-Tucker (KKT) conditions. We investigate the feasibility and scalability of the proposed method in three case studies of standardized 9-bus, 30-bus, and 300-bus test systems. Simulation results in terms of local flexibility prices are interpreted in economic terms and show the effectiveness of the proposed approach. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)