Comparison of the extracellular full-length and truncated recombinant protein A production in Escherichia coli BL21 (DE3)

     Protein A is a commercially important protein in biotechnological and medicinal applications. The great value of this protein and its applications in genetic and protein engineering and microbial researches as well as the growing use in biochemical industries, biotechnology, medicine and pharmacology, highlight the importance of the present study. In this survey the encoding genes of full-length and truncated forms of protein A were expressed in E. coli under an optimized expression condition. Optimization of the culture conditions resulted in an increase in expression and secretion of both forms of the protein, the pattern of expression and secretion levels for two forms was completely different. A minimum of 10-fold higher expression was observed for the truncated protein in comparison to that of the full-length recombinant form. Hydropathy plot of both forms of proteins showed that the missing domains in the truncated form contain groups of amino acids with high hydrophobicity score. Deletion of the terminal region could led to a higher expression level of the recombinant protein in E. coli. The function of these two proteins was studied using ELISA, which showed a higher activity for the truncated form for binding to IgG, compared to the full-length protein.

Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma

Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of FADD and FAS genes in risk of OSCC. Material and Methods: we analyze the promoter methylation status of FADD and FAS genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also, FADD and FAS genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse- Transcripts PCR. Results: Aberrant promoter methylation of FADD and FAS genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both FADD and FAS genes ( P <0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both FADD ( p <0.02) and FAS ( p <0.007) genes. Conclusions: To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the FADD and FAS genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations

Neuroprotective Effect of Coenzyme Q10 in Hippocampal Injury in Balb/c Mouse

Coenzyme Q10 is a promising agent for neuroprotection in neurodegenerative diseases. Neuroprotective effects of Coenzyme Q10 demonstrated in some neurodegenerative diseases such as Parkinson, Alzheimer and etc. Hippocampus is home of these diseases. We assayed Coenzyme Q10 effects on Hippocampal injury model and our hypothesis is that Coenzyme Q10 has Neuroprotective effects in some neurodegenerative diseases via hippocampus. For this purpose 24 Balb/c mouse took in 4 groups: Control (Without any treatment), Vehicle (Treated with sesame oil as Coenzyme Q10 vehicle), Hyppocampal injury model (Treated with Trimethyltin chlorideneurotoxin, 2.5 mg per kg IP), and test (Treated with Coenzyme Q10 after Trimethyltin chloride injection, 10 mg per kg IP for 2 weeks). After two weeks brain harvested and hippocampus tissue assayed by Nissl and Tunnel staining. Hystological study showed significantly increase of normal cells and decrease of apoptotic cells in test group after Coenzyme Q10 treatment in hippocampus. This study showed Coenzyme Q10 has protective effects in hippocampus after injury and it seems that Neuroprotective effects of Coenzyme Q10 in some neurodegenerative diseases com from that

Mediating Role of Distress Tolerance in Relationship of Emotional Maturity and Spiritual Intelligence with Adjustment to University

For downloading the full-text of this article please click here.Background and Objective: Adjustment to university is an important factor in different dimensions of students’ life such as mental- physical health and social adjustment in the fields outside the university. The aim of this study was to determine mediating role of distress tolerance in the relationship of spiritual intelligence and emotional maturity with adjustment to university.Method: In this correlation study, based on Krejsi and Murgan table, 273 students of Shahid Chamran University in Ahvaz city were selected via multistage cluster sampling method during the fall semester of the academic year of 2016-2017. The participants completed the items of Emotional Maturity Scale, Distress Tolerence Scale, Spiritual Intelligence Inventory and Adjustment to College Scale. The data were then analyzed using Pearson correlation coefficient, structural equation modeling. All ethical issues were observed in this study and the researchers declared no conflict of interests.Results: The findings showed that emotional maturity, spiritual intelligence, and distress tolerance had positive significant correlation with adjustment to college. There was also a positive significant correlation between emotional maturity as well as spiritual intelligence and distress tolerance. The proposed model for relationship between these variables has acceptable fit with a correction and removal of direct path of spiritual intelligence with adjustment to college. Direct path of emotional maturity to adjustment to college was significant. The analysis based on Bootstrapping indicated that all indirect paths were significant.Conclusion: It is proposed that to raise adjustment to university, educational programs for emotional maturity and spiritual intelligence with an emphasis on enhancing distress tolerance should be developed.For downloading the full-text of this article please click here.Please cite this article as: Sharifi Rigi A, Mehrabizade Honarmand M, Beshlideh K, Sarparast A, Khanali Nejad S, Amini Z. Mediating Role of Distress Tolerance in Relationship of Emotional Maturity and Spiritual Intelligence with Adjustment to University. J Res Relig Health. 2019; 5(1): 87- 100. doi: https://doi.org/10.22037/jrrh.v5i1.1940

نقش واسطه‌یی تحمل پریشانی در رابطه‌ی بلوغ عاطفی و هوش معنوی با سازگاری با دانشگاه در دانشجویان پسر دانشگاه شهید چمران اهواز

خلفية البحث وأهدافه: التكيف مع الحياة الجامعية هو واحد من العوامل المهمة في مختلف جوانب الحياة الطلابية كباقي العوامل بما في ذلك الصحة الجسمية والعقلية والتكيف الاجتماعي في الاجواء غير الجامعية. الغرض من هذا البحث، دراسة دور تحمل الارتباك كعامل وسيط في النضج العاطفي والذكاء المعنوي مع التكييف الجامعي. منهجية البحث: في تصميم ارتباطي، تم اختیار 273 طالباً من بين الطلاب المقيمين في القسم الداخلي بجامعة الشهيد شمران في العام الدراسي 2016-2017 وذلك بطريقة اخذ العينة العنقودية مستخدما جدول krejcie و Morgan. تم استخدام استمارة النضج العاطفي والذكاء المعنوي وتحمل الإرتباك والتكيف الجامعى لغرض جمع البيانات. تم تحليل البيانات وتجزئتها باستخدام معامل ارتباط بيرسون والنمذجة بالمعادلة البنائية (SEM). تمت مراعاة جميع الموارد الأخلاقية في هذا البحث؛ واضافة الى هذا فإن مؤلفي المقالةلم يشيروا الى اي تضارب في المصالح. الكشوفات: بناء على الكشوفات، هناك علاقة ايجابية ذات دلالة احصائية بين النضج العاطفي والذكاء المعنوي وبين التكيف الجامعي. كما ان هناك علاقة ايجابية دالة احصائية ايضا بين تحمل الارتباك والتكيف الجامعي وبين النضج العاطفي والذكاء المعنوي مع تحمل الارتباك. تشير النتائج الى ان النموذج المقترح للعلاقة بين المتغيرات المذكورة أعلاه يكون مناسبًا تمامًا مع تصحيح وإزالة المسار المباشر للذكاء المعنوي الى التكيف الجامعي. اضافة الى هذا، فإن المسار المباشر للنضج العاطفي الى التكيف الجامعي كان دالا احصائياً. كما أظهرت نتائج المعاملات غير المباشرة باستخدام Bootstrap أن جميع المسارات غير المباشرة كانت ذات دلالة احصائية. الاستنتاج: اظهرت النتائج ان الذكاء المعنوي والنضج العاطفي ومن خلال تحمل الارتباط، يؤديان الى زيادة نسبة تكيف الطالب مع الحياة الجامعية. لذلك، يُقترح أن يُدوّن برامج تعليمية للذكاء المعنوي والنضج العاطفي مع التركيز على زيادة تحمّل الارتباك من أجل زيادة توافق الطلاب مع الحياة الجامعية. &nbsp; يتم استناد المقالة على الترتيب التالي: Sharifi-Rigi A, Mehrabizade-Honarmand M, Beshlideh K, Sarparast A, Khanali-Nejad S, Amini Z. Mediating Role of Distress Tolerance in Relationship of Emotional Maturity and Spiritual Intelligence with Adjustment to University. Journal of Pizhūhish dar dīn va salāmat. 2019;5(1):87-100. https://doi.org/10.22037/jrrh.v5i1.19401Background and Objective: Adjustment to university is an important factor in different dimensions of students’ life such as mental-physical health and social adjustment in the fields outside the university. The aim of this study was to determine mediating role of distress tolerance in the relationship of spiritual intelligence and emotional maturity with adjustment to university. Methods: In this correlation study, based on Krejsi and Murgan table, 273 students of Shahid Chamran University in Ahvaz city were selected via multistage cluster sampling method during the fall semester of the academic year of 2016-2017. The participants completed the items of Emotional Maturity Scale, Distress Tolerence Scale, Spiritual Intelligence Inventory and Adjustment to College Scale. The data were then analyzed using Pearson correlation coefficient, structural equation modeling. All ethical issues were observed in this study and the researchers declared no conflict of interests. Results: The findings showed that emotional maturity, spiritual intelligence, and distress tolerance had positive significant correlation with adjustment to college. There was also a positive significant correlation between emotional maturity as well as spiritual intelligence and distress tolerance. The proposed model for relationship between these variables has acceptable fit with a correction and removal of direct path of spiritual intelligence with adjustment to college. Direct path of emotional maturity to adjustment to college was significant. The analysis based on Bootstrapping indicated that all indirect paths were significant. Conclusion: It is proposed that to raise adjustment to university, educational programs for emotional maturity and spiritual intelligence with an emphasis on enhancing distress tolerance should be developed. Please cite this article as: Sharifi-Rigi A, Mehrabizade-Honarmand M, Beshlideh K, Sarparast A, Khanali-Nejad S, Amini Z. Mediating Role of Distress Tolerance in Relationship of Emotional Maturity and Spiritual Intelligence with Adjustment to University. Journal of Pizhūhish dar dīn va salāmat. 2019;5(1):87-100. https://doi.org/10.22037/jrrh.v5i1.19401سابقه و هدف: سازگاری با دانشگاه از عوامل مهم در ابعاد مختلف زندگی دانشجویان مانند بهداشت جسمی-روانی و سازگاری اجتماعی در حوزه‌های خارج از دانشگاه است. هدف این پژوهش بررسی نقش واسطه‌یی تحمل پریشانی در رابطه‌ی بلوغ عاطفی و هوش معنوی با سازگاری با دانشگاه است. روش کار: در یک طرح همبستگی و با استفاده از روش نمونه‌گیری خوشه‌یی از بین دانشجویان پسر مجتمع خوابگاهی وابسته به دانشگاه شهید چمران اهواز، با استفاده از جدول کرجسی و مورگان، تعداد 273 دانشجوی پسر در سال تحصیلی 95-96 انتخاب شدند. در پژوهش حاضر از پرسش‌نامه‌های بلوغ عاطفی، هوش معنوی، تحمل پریشانی و سازگاری با دانشگاه برای جمع‌آوری داده‌ها استفاده شد. برای تجزیه و تحلیل داده­ها نیز از ضریب همبستگی پیرسون و الگویابی معادلات ساختاری (SEM) استفاده شد. در این پژوهش همه‌ی مسائل اخلاقی رعایت شده است و نویسندگان مقاله هیچ‌گونه تضاد منافعی گزارش نکرده‌اند. یافته‌ها: بر اساس یافته‌های به‌دست‌آمده، بلوغ عاطفی و هوش معنوی با سازگاری با دانشگاه ارتباط مثبت معنادار داشت. همچنین بین تحمل پریشانی و سازگاری با دانشگاه؛ و بین بلوغ عاطفی و هوش معنوی با تحمل پریشانی نیز ارتباط مثبت معنادار وجود داشت. نتایج نشان داد که مدل پیشنهادی با یک اصلاح و حذف مسیر مستقیم هوش معنوی به سازگاری با دانشگاه دارای برازش پذیرفتنی بود. علاوه‌براین، مسیر مستقیم بلوغ عاطفی به سازگاری با دانشگاه نیز معنی‌دار بود. همچنین، نتایج ضرایب غیرمستقیم با استفاده از بوت استراپ نشان داد که همه‌ی مسیرهای غیرمستقیم نیز معنی‌دار بود. نتیجه‌گیری: نتایج نشان می‌دهد که هوش معنوی و بلوغ عاطفی از طریق تحمل پریشانی موجب افزایش سازگاری با دانشگاه می‌شود. بنابراین، پیشنهاد می‌شود به منظور افزایش سازگاری با دانشگاه در دانشجویان، برنامه‌های آموزشی هوش معنوی و بلوغ عاطفی با تأکید بر افزایش تحمل پریشانی آنان تدوین شود. &nbsp; استناد مقاله به این صورت است: Sharifi-Rigi A, Mehrabizade-Honarmand M, Beshlideh K, Sarparast A, Khanali-Nejad S, Amini Z. Mediating Role of Distress Tolerance in Relationship of Emotional Maturity and Spiritual Intelligence with Adjustment to University. Journal of Pizhūhish dar dīn va salāmat. 2019;5(1):87-100. https://doi.org/10.22037/jrrh.v5i1.1940

Evaluation of the neuroprotective effects of electromagnetic fields and coenzyme Q10 on hippocampal injury in mouse

Electromagnetic fields (EMFs) are reported to interfere with chemical reactions involving free radical production. Coenzyme Q10 (CoQ10) is a strong antioxidant with some neuroprotective activities. The purpose of this study was to examine and compare the neuroprotective effects of EMF and CoQ10 in a mouse model of hippocampal injury. Hippocampal injury was induced in mature female mice (25–30 g), using an intraperitoneal injection of trimethyltin hydroxide (TMT; 2.5 mg/kg). The experimental groups were exposed to EMF at a frequency of 50 Hz and intensity of 5.9 mT for 7 hr daily over 1 week or treated with CoQ10 (10 mg/kg) for 2 weeks following TMT injection. A Morris water maze apparatus was used to assess learning and spatial memory. Nissl staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) tests were also performed for the histopathological analysis of the hippocampus. Antiapoptotic genes were studied, using the Western blot technique. The water maze test showed memory improvement following treatment with CoQ10 and coadministration of CoQ10 + EMF. The Nissl staining and TUNEL tests indicated a decline in necrotic and apoptotic cell count following treatment with CoQ10 and coadministration of CoQ10 + EMF. The Western blot study indicated the upregulation of antiapoptotic genes in treatment with CoQ10, as well as coadministration. Also, treatment with EMF had no significant effects on reducing damage induced by TMT in the hippocampus. According to the results, EMF had no significant neuroprotective effects in comparison with CoQ10 on hippocampal injury in mice. Nevertheless, coadministration of EMF and CoQ10 could improve the neuroprotective effects of CoQ10. keywords:CoQ10 electromagnetic fields (EMFs) neuroprotective effect trimethyltin hydroxide (TMT

Evaluation of the neuroprotective effects of electromagnetic fields and coenzyme Q 10 on hippocampal injury in mouse

Electromagnetic fields (EMFs) are reported to interfere with chemical reactions involving free radical production. Coenzyme Q(10) (CoQ10) is a strong antioxidant with some neuroprotective activities. The purpose of this study was to examine and compare the neuroprotective effects of EMF and CoQ10 in a mouse model of hippocampal injury. Hippocampal injury was induced in mature female mice (25-30g), using an intraperitoneal injection of trimethyltin hydroxide (TMT; 2.5mg/kg). The experimental groups were exposed to EMF at a frequency of 50Hz and intensity of 5.9mT for 7hr daily over 1 week or treated with CoQ10 (10mg/kg) for 2 weeks following TMT injection. A Morris water maze apparatus was used to assess learning and spatial memory. Nissl staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) tests were also performed for the histopathological analysis of the hippocampus. Antiapoptotic genes were studied, using the Western blot technique. The water maze test showed memory improvement following treatment with CoQ10 and coadministration of CoQ10+EMF. The Nissl staining and TUNEL tests indicated a decline in necrotic and apoptotic cell count following treatment with CoQ10 and coadministration of CoQ10+EMF. The Western blot study indicated the upregulation of antiapoptotic genes in treatment with CoQ10, as well as coadministration. Also, treatment with EMF had no significant effects on reducing damage induced by TMT in the hippocampus. According to the results, EMF had no significant neuroprotective effects in comparison with CoQ10 on hippocampal injury in mice. Nevertheless, coadministration of EMF and CoQ10 could improve the neuroprotective effects of CoQ10