A lisztérzékenység biopszichoszociális szemléletű áttekintése = Coeliac disease in biopsychosocial approach

A lisztérzékenység — emelkedő gyakorisága és a lehetséges szövődmények miatt — az egészségügy kiemelt kérdései közé tartozik. Felismerése, kezelése biopszichoszociális attitűdöt kíván. A jelen tanulmány keretében azokat a területeket tekintjük át, amelyek pszichológiai kérdéseket is felvetnek: a betegséggel együtt járó komorbid pszichiátriai zavarokat (depresszió, szorongás, evészavarok, súlyos pszichiátriai állapotok), a neurológiai eltéréseket és ezek lehetséges háttérmechanizmusait, az életminőséget befolyásoló tényezőket és a diétához való adherencia nehézségeit. Kitérünk a betegség menedzselésének fő szempontjaira, az állapot személyiségfejlődésre és családi életre kifejtett lehetséges hatásaira, illetve a lisztérzékenység témakörében felmerült tovább kutatandó kérdésekre (mint például szűrés, prevenció). | Coeliac disease — because of its increasing prevalence and possible adverse consequences — is a significant problem in health care systems. Diagnosis and treatment requires biopsychosocial attitude. The recent review focuses on topics including psychological aspects: comorbid psychiatric disorders (depression, anxiety, eating disorders, acute psychiatric states), neurologic alterations and the possible underlying mechanisms, factors influencing quality of life and the difficulties of adherence to diet. We also discuss the main aspects of coeliac disease management, the possible influence of the disease on personality development and family life and questions requiring further research (population screening, prevention)

Hashimoto's thyroiditis in health psychological approach

This paper aimed to present psychological correlates of Hashimoto's thyroiditis as widely as possible. Although working with patients clearly suggests that health psychological support may be needed at various points and regarding several issues, these aspects are not emphasized in the literature or treatment protocols. The author hopes that the research suggestions and treatment proposals presented, all based on scientific and practical evidence, will meet with openness by professionals. Hopefully, a more determined observation of the biopsychosocial approach will also be launched in a pathology that has hitherto been neglected from a health psychological point of view

Suspension protoplasts as useful experimental tool to study localization of GFP-tagged proteins in Arabidopsis thaliana

A routinely used protocol is described here including protoplast isolation and PEG-mediated plasmid DNA transformation followed by LSM analysis. We have isolated protoplasts from suspension cultures of wild-type Arabidopsis thaliana Col-0, than protoplasts were transformed with different constructs of AtCRK5 gene tagged with GFP. The protoplast isolation and PEG-mediated transformation process took 6-8 hours. Localization studies could be carried out by LSM microscopy in 0-24 hrs followed transformation. Using this method, we could have localized both 35S::cCRK5-GFP and g::gCRK5-GFP fusion proteins in plasma membrane of cell suspension originated protoplasts, while an N-terminal myristoylation site masked version of 35S::cCRK5-GFP and the N-terminal GFP tagged 35S::GFP-cCRK5 fusion proteins could be found in cell nuclei. Mislocalization of the two last fusion proteins is in good agreement with the fact that the N-terminal myristoylation sites of these proteins were impaired. As a conclusion, the Arabidopsis suspension derived protoplast system is very quick tool for identification of protein localization and to provide possibility to obtain preliminarily information on possible protein localization prior to plant regeneration