898 research outputs found
Dynamic acoustic field activated cell separation (DAFACS)
Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools
for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for highthroughput,
label-free, high recovery, cell and particle separation and isolation in regenerative medicine.
Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an
arbitrary size range of cells. We first demonstrate the method for the separation of particles with different
diameters between 6 and 45 μm and secondly particles of different densities in a heterogeneous medium.
The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and
low damage characteristics make this method of manipulation particularly suited for biological applications.
Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and
biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle
population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to
separate, up to 100%)
Transverse laser cooling of a thermal atomic beam of dysprosium
A thermal atomic beam of dysprosium (Dy) atoms is cooled using the
transition at 421 nm. The cooling is
done via a standing light wave orthogonal to the atomic beam. Efficient
transverse cooling to the Doppler limit is demonstrated for all observable
isotopes of dysprosium. Branching ratios to metastable states are demonstrated
to be . A scheme for enhancement of the
nonzero-nuclear-spin-isotope cooling, as well as a method for direct
identification of possible trap states, is proposed.Comment: 5 pages, 4 figures v2: 7 pages, 7 figure
Doppler cooling and trapping on forbidden transitions
Ultracold atoms at temperatures close to the recoil limit have been achieved
by extending Doppler cooling to forbidden transitions. A cloud of ^40Ca atoms
has been cooled and trapped to a temperature as low as 6 \mu K by operating a
magneto-optical trap on the spin-forbidden intercombination transition.
Quenching the long-lived excited state with an additional laser enhanced the
scattering rate by a factor of 15, while a high selectivity in velocity was
preserved. With this method more than 10% of pre-cooled atoms from a standard
magneto-optical trap have been transferred to the ultracold trap. Monte-Carlo
simulations of the cooling process are in good agreement with the experiments
Optical Clocks in Space
The performance of optical clocks has strongly progressed in recent years,
and accuracies and instabilities of 1 part in 10^18 are expected in the near
future. The operation of optical clocks in space provides new scientific and
technological opportunities. In particular, an earth-orbiting satellite
containing an ensemble of optical clocks would allow a precision measurement of
the gravitational redshift, navigation with improved precision, mapping of the
earth's gravitational potential by relativistic geodesy, and comparisons
between ground clocks.Comment: Proc. III International Conference on Particle and Fundamental
Physics in Space (SpacePart06), Beijing 19 - 21 April 2006, to appear in
Nucl. Phys.
Calculations of collisions between cold alkaline earth atoms in a weak laser field
We calculate the light-induced collisional loss of laser-cooled and trapped
magnesium atoms for detunings up to 50 atomic linewidths to the red of the
^1S_0-^1P_1 cooling transition. We evaluate loss rate coefficients due to both
radiative and nonradiative state-changing mechanisms for temperatures at and
below the Doppler cooling temperature. We solve the Schrodinger equation with a
complex potential to represent spontaneous decay, but also give analytic models
for various limits. Vibrational structure due to molecular photoassociation is
present in the trap loss spectrum. Relatively broad structure due to absorption
to the Mg_2 ^1Sigma_u state occurs for detunings larger than about 10 atomic
linewidths. Much sharper structure, especially evident at low temperature,
occurs even at smaller detunings due to of Mg_2 ^1Pi_g absorption, which is
weakly allowed due to relativistic retardation corrections to the forbidden
dipole transition strength. We also perform model studies for the other
alkaline earth species Ca, Sr, and Ba and for Yb, and find similar qualitative
behavior as for Mg.Comment: 20 pages, RevTex, 13 eps figures embedde
Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair
Tubular scaffolds which incorporate a variety of micro- and nanotopographies have a wide application potential in tissue engineering especially for the repair of spinal cord injury (SCI). We aim to produce metabolically active differentiated tissues within such tubes, as it is crucially important to evaluate the biological performance of the three-dimensional (3D) scaffold and optimize the bioprocesses for tissue culture. Because of the complex 3D configuration and the presence of various topographies, it is rarely possible to observe and analyze cells within such scaffolds in situ. Thus, we aim to develop scaled down mini-chambers as simplified in vitro simulation systems, to bridge the gap between two-dimensional (2D) cell cultures on structured substrates and three-dimensional (3D) tissue culture. The mini-chambers were manipulated to systematically simulate and evaluate the influences of gravity, topography, fluid flow, and scaffold dimension on three exemplary cell models that play a role in CNS repair (i.e., cortical astrocytes, fibroblasts, and myelinating cultures) within a tubular scaffold created by rolling up a microstructured membrane. Since we use CNS myelinating cultures, we can confirm that the scaffold does not affect neural cell differentiation. It was found that heterogeneous cell distribution within the tubular constructs was caused by a combination of gravity, fluid flow, topography, and scaffold configuration, while cell survival was influenced by scaffold length, porosity, and thickness. This research demonstrates that the mini-chambers represent a viable, novel, scale down approach for the evaluation of complex 3D scaffolds as well as providing a microbioprocessing strategy for tissue engineering and the potential repair of SCI
Colloquium: Comparison of Astrophysical and Terrestrial Frequency Standards
We have re-analyzed the stability of pulse arrival times from pulsars and
white dwarfs using several analysis tools for measuring the noise
characteristics of sampled time and frequency data. We show that the best
terrestrial artificial clocks substantially exceed the performance of
astronomical sources as time-keepers in terms of accuracy (as defined by cesium
primary frequency standards) and stability. This superiority in stability can
be directly demonstrated over time periods up to two years, where there is high
quality data for both. Beyond 2 years there is a deficiency of data for
clock/clock comparisons and both terrestrial and astronomical clocks show equal
performance being equally limited by the quality of the reference timescales
used to make the comparisons. Nonetheless, we show that detailed accuracy
evaluations of modern terrestrial clocks imply that these new clocks are likely
to have a stability better than any astronomical source up to comparison times
of at least hundreds of years. This article is intended to provide a correct
appreciation of the relative merits of natural and artificial clocks. The use
of natural clocks as tests of physics under the most extreme conditions is
entirely appropriate; however, the contention that these natural clocks,
particularly white dwarfs, can compete as timekeepers against devices
constructed by mankind is shown to be doubtful.Comment: 9 pages, 2 figures; presented at the International Frequency Control
Symposium, Newport Beach, Calif., June, 2010; presented at Pulsar Conference
2010, October 12th, Sardinia; accepted 13th September 2010 for publication in
Reviews of Modern Physic
Absolute Frequency Measurements of the Hg^+ and Ca Optical Clock Transitions with a Femtosecond Laser
The frequency comb created by a femtosecond mode-locked laser and a
microstructured fiber is used to phase coherently measure the frequencies of
both the Hg^+ and Ca optical standards with respect to the SI second as
realized at NIST. We find the transition frequencies to be f_Hg=1 064 721 609
899 143(10) Hz and f_Ca=455 986 240 494 158(26) Hz, respectively. In addition
to the unprecedented precision demonstrated here, this work is the precursor to
all-optical atomic clocks based on the Hg^+ and Ca standards. Furthermore, when
combined with previous measurements, we find no time variations of these atomic
frequencies within the uncertainties of |(df_Ca/dt)/f_Ca| < 8 x 10^{-14}
yr^{-1}, and |(df_Hg/dt)/f_Hg|< 30 x 10^{-14} yr^{-1}.Comment: 6 pages, including 4 figures. RevTex 4. Submitted to Phys. Rev. Let
The Putative AKH Receptor of the Tobacco Hornworm, Manduca sexta, and Its Expression
Adipokinetic hormones are peptide hormones that mobilize lipids and/or carbohydrates for flight in adult insects and activate glycogen Phosphorylase in larvae during starvation and during molt. We previously examined the functional roles of adipokinetic hormone in Manduca sexta L. (Lepidoptera: Sphingidae). Here we report the cloning of the full-length cDNA encoding the putative adipokinetic hormone receptor from the fat body of M. sexta. The sequence analysis shows that the deduced amino acid sequence shares common motifs of G protein-coupled receptors, by having seven hydrophobic transmembrane segments. We examined the mRNA expression pattern of the adipokinetic hormone receptor by quantitative Real-Time PCR in fat body during development and in different tissues and found the strongest expression in fat body of larvae two days after molt to the fifth instar. We discuss these results in relation to some of our earlier results. We also compare the M. sexta adipokinetic hormone receptor with the known adipokinetic hormone receptors of other insects and with gonadotropin releasing hormone-like receptors of invertebrates
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