Quantification of leptospiral DNA in tissues of hamsters infected with strains of <i>L</i>. <i>interrogans</i> serovars Manilae or Hebdomadis at 96 h pi.

<p>Leptospiral DNA was quantified by real-time PCR targeting leptospiral <i>flaB</i> gene in blood (A), kidney (B), liver (C), and lung (D) tissues of hamsters infected with strains of <i>L</i>. <i>interrogans</i> serovars Manilae (filled boxes) or Hebdomadis (open boxes) at 96 h pi. The bottom, median, and top lines of the box indicate the 25th, 50th, and 75th percentiles, respectively. The vertical line with whiskers shows the range of values. Experiments were performed in duplicate using two independently extracted DNA samples for each tissue of infected hamsters; each dot indicates the average of two experiments. Data out of the range of values were excluded from statistical analysis. **, p < 0.01.</p

Quantification of expression of cytokine genes in the blood of hamsters infected with <i>L</i>. <i>interrogans</i> serovars Manilae or Hebdomadis at 96 h pi.

<p>Cytokine gene expression in the blood of hamsters infected with strains of <i>L</i>. <i>interrogans</i> serovars Manilae (filled boxes) or Hebdomadis (open boxes) was quantified at 96 h pi using real-time PCR (2^−ΔΔCt method). The bottom, median, and top lines of the box indicate the 25th, 50th, and 75th percentiles, respectively. The vertical line with whiskers shows the range of values. Experiments were performed in duplicate using two independently extracted RNA samples for each hamster; each dot indicates the average of two experiments and gene expression relative to control hamsters. The dotted line indicates the expression level in control hamsters (for calibration). Data out of the range of values were excluded from statistical analysis. *, p < 0.05.</p

Comparison of Bacterial Burden and Cytokine Gene Expression in Golden Hamsters in Early Phase of Infection with Two Different Strains of <i>Leptospira interrogans</i>

<div><p>Leptospirosis, a zoonotic infection with worldwide prevalence, is caused by pathogenic spirochaetes of <i>Leptospira</i> spp., and exhibits an extremely broad clinical spectrum in human patients. Although previous studies indicated that specific serovars or genotypes of <i>Leptospira</i> spp. were associated with severe leptospirosis or its outbreak, the mechanism underlying the difference in virulence of the various <i>Leptospira</i> serotypes or genotypes remains unclear. The present study addresses this question by measuring and comparing bacterial burden and cytokine gene expression in hamsters infected with strains of two <i>L</i>. <i>interrogans</i> serovars Manilae (highly virulent) and Hebdomadis (less virulent). The histopathology of kidney, liver, and lung tissues was also investigated in infected hamsters. A significantly higher bacterial burden was observed in liver tissues of hamsters infected with serovar Manilae than those infected with serovar Hebdomadis (p < 0.01). The average copy number of the leptospiral genome was 1,302 and 20,559 in blood and liver, respectively, of hamsters infected with serovar Manilae and 1,340 and 4,896, respectively, in hamsters infected with serovar Hebdomadis. The expression levels of <i>mip1alpha</i> in blood; <i>tgfbeta</i>, <i>il1beta</i>, <i>mip1alpha</i>, <i>il10</i>, <i>tnfalpha</i> and <i>cox2</i> in liver; and <i>tgfbeta</i>, <i>il6</i>, <i>tnfalpha</i> and <i>cox2</i> in lung tissue were significantly higher in hamsters infected with serovar Manilae than those infected with serovar Hebdomadis (p < 0.05). In addition, infection with serovar Manilae resulted in a significantly larger number of hamsters with <i>tnfalpha</i> upregulation (p = 0.04). Severe distortion of tubular cell arrangement and disruption of renal tubules in kidney tissues and hemorrhage in lung tissues were observed in Manilae-infected hamsters. These results demonstrate that serovar Manilae multiplied more efficiently in liver tissues and induced significantly higher expression of genes encoding pro- and anti-inflammatory cytokines than serovar Hebdomadis even in tissues for which a significant difference in leptospiral load was not observed. In addition, our results suggest a serovar Manilae-specific mechanism responsible for inducing severe damage in kidneys and hemorrhage in lung.</p></div

Quantification of cytokine gene expression in kidney tissues of hamsters infected with <i>L</i>. <i>interrogans</i> serovars Manilae or Hebdomadis at 96 h pi.

<p>Cytokine gene expression in kidney tissues of hamsters infected with strains of <i>L</i>. <i>interrogans</i> serovars Manilae (filled boxes) or Hebdomadis (open boxes) was quantified at 96 h pi using real-time PCR (2^−ΔΔCt method). The bottom, median, and top lines of the box indicate the 25th, 50th, and 75th percentiles, respectively. The vertical line with whiskers shows the range of values. The expression of cytokine genes in panel B was below the detection limit in control hamsters. Experiments were performed in duplicate using two independently extracted RNA samples for each hamster; each circle indicates the average of two experiments and gene expression relative to control hamsters (A) or hamsters infected with serovar Hebdomadis (B). The dotted line indicates the expression level in control (A) or Hebdomadis-infected (B) hamsters. Data out of the range of values were excluded from statistical analysis.</p

Quantification of cytokine gene expression in lung tissues of hamsters infected with <i>L</i>. <i>interrogans</i> serovars Manilae or Hebdomadis at 96 h pi.

<p>Cytokine gene expression in lung tissues of hamsters infected with strains of <i>L</i>. <i>interrogans</i> serovars Manilae (filled boxes) or Hebdomadis (open boxes) was quantified at 96 h pi using real-time PCR (2^−ΔΔCt method). The bottom, median, and top lines of the box indicate the 25th, 50th, and 75th percentiles, respectively. The vertical line with whiskers shows the range of values. The expression of cytokine genes in panel B was below the detection limit in control hamsters. Experiments were performed in duplicate using two independently extracted RNA samples for each hamster; each circle in the plots indicates the average of two experiments and gene expression relative to control hamsters (A) or hamsters infected with serovar Hebdomadis (B). Dotted line indicates the expression level in control (A) or Hebdomadis-infected (B) hamsters. Data out of the range of values were excluded from statistical analysis. *, p < 0.05; **, p < 0.01; in panel B, the asterisks indicate significant differences in ΔCt values (Ct value of target gene − Ct value of <i>rpl18</i>) between hamsters infected with the serovars Manilae and Hebdomadis.</p

Collision detection on transmission lines with optical interferometer

V diplomski nalogi skušamo ugotoviti, v kolikšni meri je možno zaznavati in klasificirati trke na jeklenicah daljnovodov z optičnim interferometrom. Na začetku predstavimo osnovne pojme interferometrije in opišemo uporabljen optični interferometer. V jedru diplomske naloge natančneje opišemo eksperimentalni protokol in obdelavo signalov. Nadaljujemo z implementacijo algoritmov za segmentacijo in klasifikacijo zajetih signalov ter predstavimo dobljene rezultate. Segmentacijo izvedemo v domeni števila prehodov signala skozi ničlo, za klasifikacijo pa uporabimo večplastno nevronsko mrežo z algoritmom vzvratnega učenja. Rezultati študije nakazujejo, da sta implementirani segmentacija in klasifikacija uspešni v 77 % izvedenih trkov različnih predmetov.We analyse feasibility of collision detection on transmission lines with optical interferometer. We first provide a brief introduction into interferometry, along with a description of the optical interferometer used for measurements in this study. Afterwards, we describe the conducted experimental protocol and signal processing methodology. The focus is on implementation of algorithms for signal segmentation and collision classification. We used zero-crossing algorithm to transform signals into segmentation domain. Classification of collisions is done with a multilayer neural network trained by the backpropagation algorithm. The results demonstrate an average success rate of 77% for segmentation and classification of collision with five different objects

Sistematización de la experiencia de un ambiente de aprendizaje enriquecido por TIC durante la práctica clínica en fisioterapia cardiopulmonar en un hospital de nivel II de la ciudad de Cali

Esta investigación se centra en la caracterización de la experiencia de 4 estudiantes de fisioterapia de IX semestre de la Institución Universitaria Escuela Nacional del Deporte (IUEND) durante la implementación de un ambiente de aprendizaje enriquecido con Tecnologías de la Información y la Comunicación (TIC) en la práctica clínico – asistencial en Salud Cardiopulmonar; la cual se fundamenta en el hacer y pone a prueba las bases conceptuales del ciclo de fundamentación; todo esto con el fin de identificar las experiencias significativas que facilitan el aprendizaje y desarrollo de competencias clínicas, además analizar si este tipo de estrategias de enseñanza -aprendizaje permite al estudiante y al docente asesor superar inconvenientes propios de la práctica clínica como: optimizar tiempos de atención a pacientes, estudio independiente y trabajo colaborativo, retomar e integrar gran cantidad de conceptos y procedimientos aprendidos en IV semestre con las nuevas experiencias y la realidad del paciente; y a la vez cumplir con funciones administrativas propios del rol del fisioterapeuta asistencial (estadística, indicadores, desarrollo de guías, etc.) que dificultan el proceso de aprendizaje; concluyendo que los ambientes mediados por TIC pueden lograr superar estas dificultades y favorecer finalmente el aprendizaje significativo (juicio clínico), en el que se fundamenta el ciclo de práctica profesional

assay information of the mouse tissue samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse tissue samples for the standard protocol of the HeliScopeCAGE protoco

assay information of the mouse qualitycontrol samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for mouse quality control samples for the standard protocol of the HeliScopeCAGE protoco

DRA001027

List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers of the FANTOM5 samples, sequences and genomic coordinations. Files are in tab-delimited format, which includes * Library ID * FFID * BioSamples accession number * DRA experiment accession number * DRA run accession numbers * DRA analysis accession number for genomic coordination (BAM file) * DRA analysis accession number for CTSS (BED file) * Experiment method (CAGE/RNA-Seq/sRNA-Seq