In Vitro Cytotoxicity Assays Of Solid Lipid Nanoparticles In Epithelial And Dermal Cells

In recent years, the interest in nanostructured systems to drug delivery has increased because they offer several advantages over conventional dosage forms. Solid Lipid Nanoparticles (SLN) have been highlighted among these systems because they have advantages such as high physical stability, protection against drug degradation and ease of scale-up and manufacturing, without using organic solvent. The aim of this work was to evaluate the potential of SLN, by in vitro cytotoxicity assays, for dermal drug delivery. SLN of three different lipids were prepared by hot high pressure homogenization and the cytotoxicity was assessed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test in mouse 3T3 fibroblasts and human HaCaT keratinocytes. SLN showed no cytotoxic potential suggesting a great potential for dermal application.3041Mishra, B., Patel, B.B., Tiwari, S., (2010) Nanomed.-Nanotechnol. Biol. Med., 6, p. 9Mehnert, W., Mäder, K., (2001) Adv. Drug Deliv. Rev., 47, p. 165Pardeike, J., Hommoss, A., Müller, R.H., (2009) Int. J. Pharm., 366, p. 170Lewinski, N., Colvin, V., Drezek, R., (2008) Small, 4, p. 26Schöler, N., Hahn, H., Müller, R.H., Liesenfeld, O., (2002) Int. J. Pharm., 231, p. 167Müller, R.H., Maassen, S., Schwarz, C., Mehnert, W., (1997) J. Control. Release, 47, p. 261Shöler, N., Hahn, H., Müller, R.H., Liesenfeld, O., (2002) Int. J. Pharm., 231, p. 167Weyenberg, W., Filev, P., Plas, D.V., Vandervoort, J., Smet, K.D., Sollie, P., Ludwig, A., (2007) Int. J. Pharm., 337, p. 291Kristl, J., Teskac, K., Milek, M., Rascan, I.M., (2008) Toxicol. Appl.Pharmacol., 232, p. 218Mosmann, T., (1983) J. Immunol. Methods, 65, p. 55Marcato, P.D., Caverzan, J., Rossi-Bergmann, B., Pinto, E.F., MacHado, D., Silva, R.A., Justos, G.Z., Durán, N., (2011) J. Nanosci. Nanotechnol

Simultaneous multi-telescope observations of FRB 121102

International audienceWe present 11 detections of FRB 121102 in ∼3 h of observations during its ‘active’ period on the 10th of 2019 September. The detections were made using the newly deployed MeerTRAP system and single pulse detection pipeline at the MeerKAT radio telescope in South Africa. Fortuitously, the Nançay radio telescope observations on this day overlapped with the last hour of MeerKAT observations and resulted in four simultaneous detections. The observations with MeerKAT’s wide band receiver, which extends down to relatively low frequencies (900–1670 MHz usable L-band range), have allowed us to get a detailed look at the complex frequency structure, intensity variations, and frequency-dependent sub-pulse drifting. The drift rates we measure for the full-band and sub-banded data are consistent with those published between 600 and 6500 MHz with a slope of −0.147 ± 0.014 ms^−1. Two of the detected bursts exhibit fainter ‘precursors’ separated from the brighter main pulse by ∼28 and ∼34 ms. A follow-up multi-telescope campaign on the 6th and 8th of 2019 October to better understand these frequency drifts and structures over a wide and continuous band was undertaken. No detections resulted, indicating that the source was ‘inactive’ over a broad frequency range during this time