23,250 research outputs found
Conformational studies of various hemoglobins by natural-abundance 13C NMR spectroscopy
Studies of variously liganded hemoglobins (both from human and rabbit) by natural-abundance 13C NMR spectroscopy have revealed apparent conformational differences that have been interpreted on the basis of two quaternary structures for the α2ß2 tetramer, and variable tertiary structures for the individual α and ß subunits. In solution, rabbit hemoglobins appear to have somewhat more flexibility than human hemoglobins
Transient heat and mass transfer analysis of supercritical cryogenic storage systems with spherical static heaters Final report
Transient heat and mass transfer analysis of supercritical cryogenic storage systems with spherical static heaters by computer progra
Outer-Sphere Effects on Reduction Potentials of Copper Sites in Proteins: The Curious Case of High Potential Type 2 C112D/M121E Pseudomonas aeruginosa Azurin
Redox and spectroscopic (electronic absorption, multifrequency electron paramagnetic resonance (EPR), and X-ray absorption) properties together with X-ray crystal structures are reported for the type 2 Cu^(II) C112D/M121E variant of Pseudomonas aeruginosa azurin. The results suggest that Cu^(II) is constrained from interaction with the proximal glutamate; this structural frustration implies a “rack” mechanism for the 290 mV (vs NHE) reduction potential measured at neutral pH. At high pH (~9), hydrogen bonding in the outer coordination sphere is perturbed to allow axial glutamate ligation to Cu^(II), with a decrease in potential to 119 mV. These results highlight the role played by outer-sphere interactions, and the structural constraints they impose, in determining the redox behavior of transition metal protein cofactors
A dynamic model of Venus's gravity field
Unlike Earth, long wavelength gravity anomalies and topography correlate well on Venus. Venus's admittance curve from spherical harmonic degree 2 to 18 is inconsistent with either Airy or Pratt isostasy, but is consistent with dynamic support from mantle convection. A model using whole mantle flow and a high viscosity near surface layer overlying a constant viscosity mantle reproduces this admittance curve. On Earth, the effective viscosity deduced from geoid modeling increases by a factor of 300 from the asthenosphere to the lower mantle. These viscosity estimates may be biased by the neglect of lateral variations in mantle viscosity associated with hot plumes and cold subducted slabs. The different effective viscosity profiles for Earth and Venus may reflect their convective styles, with tectonism and mantle heat transport dominated by hot plumes on Venus and by subducted slabs on Earth. Convection at degree 2 appears much stronger on Earth than on Venus. A degree 2 convective structure may be unstable on Venus, but may have been stabilized on Earth by the insulating effects of the Pangean supercontinental assemblage
High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins
Site-directed mutagenesis of Pseudomonas aeruginosa azurin
C112D at the M121 position has afforded a series of proteins with
elevated Cu^(II/I) reduction potentials relative to the CuII aquo ion.
The high potential and low axial hyperfine splitting (Cu^(II) electron
paramagnetic resonance A|) of the C112D/M121L protein are
remarkably similar to features normally associated with type 1
copper centers
The effect of parallel static and microwave electric fields on excited hydrogen atoms
Motivated by recent experiments we analyse the classical dynamics of a
hydrogen atom in parallel static and microwave electric fields. Using an
appropriate representation and averaging approximations we show that resonant
ionisation is controlled by a separatrix, and provide necessary conditions for
a dynamical resonance to affect the ionisation probability.
The position of the dynamical resonance is computed using a high-order
perturbation series, and estimate its radius of convergence. We show that the
position of the dynamical resonance does not coincide precisely with the
ionisation maxima, and that the field switch-on time can dramatically affect
the ionisation signal which, for long switch times, reflects the shape of an
incipient homoclinic. Similarly, the resonance ionisation time can reflect the
time-scale of the separatrix motion, which is therefore longer than
conventional static field Stark ionisation. We explain why these effects should
be observed in the quantum dynamics.
PACs: 32.80.Rm, 33.40.+f, 34.10.+x, 05.45.Ac, 05.45.MtComment: 47 pages, 20 figure
Photometric study of southern SU UMa-type dwarf novae and candidates -- III: NSV 10934, MM Sco, AB Nor, CAL 86
We photometrically observed four southern dwarf novae in outburst (NSV 10934,
MM Sco, AB Nor and CAL 86). NSV 10934 was confirmed to be an SU UMa-type dwarf
nova with a mean superhump period of 0.07478(1) d. This star also showed
transient appearance of quasi-periodic oscillations (QPOs) during the final
growing stage of the superhumps. Combined with the recent theoretical
interpretation and with the rather unusual rapid terminal fading of normal
outbursts, NSV 10934 may be a candidate intermediate polar showing SU UMa-type
properties. The mean superhump periods of MM Sco and AB Nor were determined to
be 0.06136(4) d and 0.08438(2) d, respectively. We suggest that AB Nor belongs
to a rather rare class of long-period SU UMa-type dwarf novae with low
mass-transfer rates. We also observed an outburst of the suspected SU UMa-type
dwarf nova CAL 86. We identified this outburst as a normal outburst and
determined the mean decline rate of 1.1 mag/d.Comment: 13 pages, 23 figures, to appear in MNRAS. For more information, see
http://www.kusastro.kyoto-u.ac.jp/vsnet
Long-range electron transfer in structurally engineered pentaammineruthenium (histidine-62) cytochrome c
In many biological processes, long-range electron transfer (ET) plays a key role. When the three-dimensional structures of proteins are accurately known, use of modified proteins and protein-protein complexes provides an experimental approach to study ET rates between two metal centers. For Ru(His)- modified proteins, the introduction of histidine residues at any desired surface location by site-directed mutagenesis opens the way for systematic investigations of ET pathways
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