Determining effects of an all weather logging road on winter woodland caribou habitat use in south-eastern Manitoba

The Owl Lake boreal woodland caribou population is the most southerly population in Manitoba. It is provincially ranked as a High Conservation Concern Population. Forestry operations exist in the area and there are plans for further forest harvest and renewal. The Happy Lake logging road is the only main access through the Owl Lake winter range. This logging road is currently closed to the public and access is limited to forestry operations during specific times of the year. An integrated forestry/caribou management strategy for the area provides for the maintenance of minimum areas of functional habitat. Habitat quality along the road was compared to habitat quality in the winter core use areas, within the winter range and outside the winter range. To evaluate the extent of functional habitat near the road, we conducted animal location and movement analysis using GPS data collected from January 2002 to March 2006. Habitat quality in the winter range, core use areas and along the road were assessed and found to be similar. Analysis of caribou locations and movement illustrate less use of high quality habitat adjacent to the Happy Lake Road. Loss of functional habitat is suggested to occur within 1 kilometre of the road. This potential loss of functional habitat should be incorporated into integrated forestry and caribou conservation strategies. Road management is recommended to minimize the potential sensory disturbance and associated impacts of all weather access on boreal woodland caribou

Endangered Oarisma poweshiek larvae vary their graminoid forage in Manitoba, Canada

The Poweshiek skipperling (Oarisma poweshiek) is endemic to the tall grass prairie in North America and is now critically endangered globally. Existing populations are scattered among tall grass prairie remnants. However, the host food plants eaten by Poweshiek skipperling larvae, the vegetative and microclimatic descriptions of immature and adult microhabitats, and O. poweshiek behaviour in Manitoba are unknown. We followed Poweshiek skipperling adults in their natural habitat to locate microhabitats where eggs were laid and to observe larval foraging behaviour and development. We measured vegetative, structural, and microclimatic characteristics of microhabitats used by immatures and documented the host species larvae consumed, their general behaviour (on plants; movement within microhabitats), and their developmental schedules. Larvae ate Andropogon gerardi, Muhlenbergia richardsonis, Sporobolus heterolepis, and Schizachyrium scoparium (all Poaceae) in natural tall grass prairie. Larvae appeared to navigate microhabitats to locate host food plants, alternating between shoots of various species throughout their development. Microhabitats seemed to be more open, with drier microclimates, than areas where eggs were not laid. This improved understanding of larval feeding patterns, adult behaviours, and microhabitat attributes may help local grassland stewards and researchers reduce the list of possible causes of decline and identify potential solutions to recover the Poweshiek skipperling."Financial support was provided by the Canadian Wildlife Service (facilitated by M.C.), the Nature Conservancy of Canada, the University of Winnipeg, and Wildlife, Fisheries and Resource Enforcement Branch, Government of Manitoba (W.W.) and is much appreciated."https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9A3C308673F7CC950FE5875879253A66/S0008347X22000347a.pdf

An assessment of prairie management practices for maintaining habitat quality for the endangered Poweshiek skipperling butterfly in Canada

The Poweshiek skipperling (Oarisma poweshiek) was once a common prairie butterfly in central North America, but is now critically endangered in Canada and the United States. The Poweshiek skipperling is confined to the largest remaining tall grass prairie in Canada, which is currently managed using grazing and fire to maintain prairie habitat and prevent forest and shrub encroachment. To support re-introduction, restocking, and recovery of this critically endangered species, it is necessary to understand the habitat conditions preferred by skipperlings. By surveying prairie sites with Poweshiek skipperling across age and treatment categories (1–2 y since burn, 4–6 y since burn, .15 y since burn, and grazing), we identified commonly-used nectar plants and recorded physical variables known to influence plant diversity. We measured soil variables, including macronutrients, compaction, pH, texture, and moisture content, and used multivariate statistics to test for significant differences in site characteristics and plant community across treatments. For each site, we captured plant diversity, abundance, and cover, as well as total plant biomass. We found the oldest burn sites had the fewest skipperlings. Intermediate burn sites and lightly grazed sites contained the most skipperlings. In 95% of nectaring observations, adult skipperlings were feeding on Black-eyed Susan (Rudbeckia hirta), upland white aster (Solidago ptarmicoides), and Self-heal (Prunella vulgaris). Characteristics in the physical components of sites and plant communities did not significantly differ across treatment types for most variables with the exception of the oldest burns, which showed significantly higher levels of soil compaction, live biomass, bare ground, less flowering species during the flight period, less flowering stems, and increased presence of nonnative and invasive species. Poweshiek skipperling is at high risk of imminent extirpation, and we recommend that the management regimes be adjusted to plan for a consistent supply of habitat conditions and plant species composition exemplified by our intermediate burn sites. We suggest fire return intervals of 4–6 y using patch burns in combination with grazing, permitted at times that minimize the impact on immature stages of Poweshiek skipperling. This management is needed to provide suitable habitat conditions to maintain the current population and allow for successful restocking and recovery."This work was supported by research funding from Nature Conservancy of Canada, Manitoba Conservation Data Center and the University of Winnipeg."https://bioone.org/journals/the-american-midland-naturalist/volume-188/issue-1/0003-0031-188.1.74/An-Assessment-of-Prairie-Management-Practices-for-Maintaining-Habitat-Quality/10.1674/0003-0031-188.1.74.shor

\u3ci\u3eMedicine Meets Virtual Reality 21\u3c/i\u3e

Editors: James D. Westwood, Susan W. Westwood, Li Felländer-Tsai, Cali M. Fidopiastis, Randy S. Haluck, Richard A. Robb, Steven Senger, Kirby G. Vosburgh. Chapter, Varying the Speed of Perceived Self-Motion Affects Postural Control During Locomotion, co-authored by Joshua Pickhinke, Jung Hung Chien, Mukul Mukherjee, UNO faculty and staff members. Virtual reality environments have been used to show the importance of perception of self-motion in controlling posture and gait. In this study, the authors used a virtual reality environment to investigate whether varying optical flow speed had any effect on postural control during locomotion. Healthy young adult participants walked under two conditions, with optical flow matching their preferred walking speed, and with a randomly varying optic flow speed compared to their preferred walking speed. Exposure to the varying optic flow increased the variability in their postural control as measured by area of COP when compared with the matched speed condition. If perception of self-motion becomes less predictable, postural control during locomotion becomes more variable and possibly riskier.https://digitalcommons.unomaha.edu/facultybooks/1261/thumbnail.jp

Egg Laying Behaviour and Larval Shelter-Construction Patterns of the endangered Mottled Duskywing (Erynnis martialis) in Canada

The Mottled duskywing (Erynnis martialis) butterfly is endangered, living in pine forests and oak barrens in Canada and the eastern United States of America. While host plants and larval behaviour is documented in Mottled duskywing’s eastern range, these life components are poorly known in Manitoba. We observed adult behaviour, host plant species used and larval foraging to better understand these biological aspects of E. martialis. We observed eggs laid exclusively on Ceanothus herbaceus, and larvae consuming C. herbaceus in leaf shelters near the periphery of plants. Early instar larvae tied leaves together with cells of silk creating partly open shelters while later instar larvae completely sealed shelters. Shelters constructed out of young leaves at the edge of plants are likely easier to digest. Later-instar larvae may nocturnally harvest food to consume in shelters during the day to reduce predation risk. Larvae were found in clearings adjacent to Pinus banksiana dominated forests, with these openings likely providing suitable microhabitats for egg development and larval feeding. We observed newly emerged adults during weeks 1 to 5 of the flight period; eggs, larvae and adults overlapped. We recommend direct observations of larval foraging—during the day and night, as well as transitions into and out of diapause—to more accurately describe their behaviour and physiology. We started to characterise microhabitats, however further research is needed. Our research may help to guide critical habitat designations, leading to successful Mottled duskywing recovery in Manitoba."Funding was provided by the Canadian Wildlife Service (Environment and Climate Change Canada, Government of Canada) and an Alliance Grant from the Natural Sciences and Engineering Research Council of Canada. JH thanks the support for publication fees by the University of Winnipeg Graduate Student Travel / Publication Grant."https://bioone.org/journals/the-journal-of-the-lepidopterists-society/volume-76/issue-1/lepi.76i1.a6/Egg-Laying-Behaviour-and-Larval-Shelter-Construction-Patterns-of-the/10.18473/lepi.76i1.a6.shor

TPM: Cloud-Based Tele PTSD Monitor Using Multi-Dimensional Information

An automated system that can remotely and non-intrusively screen individuals at high risk for Post-Traumatic Stress Disorder (PTSD) and monitor their progress during treatment would be desired by many Veterans Affairs (VAs) as well as other PTSD treatment and research organizations. In this paper, we present an automated, cloud-based Tele-PTSD Monitor (TPM) system based on the fusion of multiple sources of information. The TPM system can be hosted in a cloud environment and accessed through landline or cell phones, or on the Internet through a web portal or mobile application (app)

A Voice-Based Automated System for PTSD Screening and Monitoring

Comprehensive evaluation of PTSD includes diagnostic interviews, self-report testing, and physiological reactivity measures. It is often difficult and costly to diagnose PTSD due to patient access and the variability in symptoms presented. Additionally, potential patients are often reluctant to seek help due to the stigma associated with the disorder. A voice-based automated system that is able to remotely screen individuals at high risk for PTSD and monitor their symptoms during treatment has the potential to make great strides in alleviating the barriers to cost effective PTSD assessment and progress monitoring. In this paper we present a voice-based automated Tele-PTSD Monitor (TPM) system currently in development, designed to remotely screen, and provide assistance to clinicians in diagnosing PTSD. The TPM system can be accessed via a Public Switched Telephone Network (PSTN) or the Internet. The acquired voice data is then sent to a secure server to invoke the PTSD Scoring Engine (PTSD-SE) where a PTSD mental health score is computed. If the score exceeds a predefined threshold, the system will notify clinicians (via email or short message service) for confirmation and/or an appropriate follow-up assessment and intervention. The TPM system requires only voice input and performs computer-based automated PTSD scoring, resulting in low cost and easy field-deployment. The concept of the TPM system was supported using a limited dataset with an average detection accuracy of up to 95.88%

Autophagic flux blockage by accumulation of weakly basic tenovins leads to elimination of B-Raf mutant tumour cells that survive vemurafenib

This work was supported by five grants to Sonia Laín: Vetenskapsrådet (VR) 521-2014-3341, Cancerfonden (Swedish Cancer Society) 150393, CAN 2014/702, Association for International Cancer Research (AICR) 130086, Barncancerfonden (Swedish Childhood Cancer Foundation) TJ-2014-0038, Barncancerfonden (Swedish Childhood Cancer Foundation) PR-2014-0038; two grants to Ravi Bhatia: Leukemia and Lymphoma Society (LLS) 6137-14 and NIH R01 CA95684; one grant to David P Lane: Vetenskapsrådet (VR) 538-2013-8807; one grant to Marcus J G W Ladds: Karolinska Institute KID Doctoral Student Funding; one grant to Gergana Popova: Karolinska Institutet KID Doctoral Student Funding; two grants to Nicholas J Westwood: Cancer Research UK C21383 and Cancer Research UK A6950; two grants to Gerald McInerney: Vetenskapsrådet (VR) 621-2014-4718 and Cancerfonden (Swedish Cancer Society) 150393, CAN 2015/751; and four grants to Emmet McCormack: Kreftforeningen 182735, Kreftforeningen 732200, Halse Vest 911884, Halse Vest 911789.Tenovin-6 is the most studied member of a family of small molecules with antitumour activity in vivo. Previously, it has been determined that part of the effects of tenovin-6 associate with its ability to inhibit SirT1 and activate p53. However, tenovin-6 has also been shown to modulate autophagic flux. Here we show that blockage of autophagic flux occurs in a variety of cell lines in response to certain tenovins, that autophagy blockage occurs regardless of the effect of tenovins on SirT1 or p53, and that this blockage is dependent on the aliphatic tertiary amine side chain of these molecules. Additionally, we evaluate the contribution of this tertiary amine to the elimination of proliferating melanoma cells in culture. We also demonstrate that the presence of the tertiary amine is sufficient to lead to death of tumour cells arrested in G1 phase following vemurafenib treatment. We conclude that blockage of autophagic flux by tenovins is necessary to eliminate melanoma cells that survive B-Raf inhibition and achieve total tumour cell kill and that autophagy blockage can be achieved at a lower concentration than by chloroquine. This observation is of great relevance as relapse and resistance are frequently observed in cancer patients treated with B-Raf inhibitors.Publisher PDFPeer reviewe