A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

First record of Culex (Culex) bidens (Diptera: Culicidae) in Colombia: Taxonomic and epidemiological implications

Arbovirus transmission cycles must be studied locally since both vectors and hosts vary in different regions. Colombia has a highly diverse mosquito fauna. Culex (Culex) bidens is reported here for the first time in Colombia. Because Cx. bidens Dyar and Knab and Cx. declarator Dyar and Knab share a close taxonomic history and because it is difficult to differentiate between them, a morphological and molecular comparison was performed. The male genitalia of three specimens of Cx. bidens from Colombia were mounted on microscope slides and morphologically compared with the male genitalia of Cx. declarator also from Colombia. In Cx. bidens, the individual teeth of the lateral plate are long, straight, laterally directed and sharply pointed; in Cx. declarator these teeth are robust, curved, with convex margins and bluntly rounded. Moreover, DNA was extracted from the same specimens and a fragment of the cytochrome c oxidase subunit I mitochondrial gene was amplified and sequenced. Neither Cx. bidens nor Cx. declarator were clustered in the Neighbour-joining topology, with K2P interspecific divergence between 0.15–1.45%. The circulation of Eastern Equine Encephalitis Virus in Colombia was reported since 1957 and Cx. bidens was suspected to be the vector of this virus during an epizootic in Argentina in 1988. Hybridization between species of the subgenus Culex has been demonstrated, hence the degree of reproductive isolation between Cx. bidens and Cx. declarator should be investigated, as well as their taxonomic status, because they only can be discriminated by a single male genitalic feature and not by nuclear or mitochondrial markers.Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; ArgentinaFil: Hoyos-López, Richard. Universidad del Sinú; Colombi

Secuencias de 658 pares de bases (fragmento barcode) del gen citocromo c oxidasa subunidad I (COI) de Culex bidens de Colombia

Arbovirus transmission cycles must be studied locally since both vectors and hosts vary in different regions. Colombia has a highly diverse mosquito fauna. Culex (Culex) bidens is reported here for the first time in Colombia. Because Cx. bidens Dyar and Knab and Cx. declarator Dyar and Knab share a close taxonomic history and because it is difficult to differentiate between them, a morphological and molecular comparison was performed. The male genitalia of three specimens of Cx. bidens from Colombia were mounted on microscope slides and morphologically compared with the male genitalia of Cx. declarator also from Colombia. In Cx. bidens, the individual teeth of the lateral plate are long, straight, laterally directed and sharply pointed; in Cx. declarator these teeth are robust, curved, with convex margins and bluntly rounded. Moreover, DNA was extracted from the same specimens and a fragment of the cytochrome c oxidase subunit I mitochondrial gene was amplified and sequenced. Neither Cx. bidens nor Cx. declarator were clustered in the Neighbour-joining topology, with K2P interspecific divergence between 0.15–1.45%. The circulation of Eastern Equine Encephalitis Virus in Colombia was reported since 1957 and Cx. bidens was suspected to be the vector of this virus during an epizootic in Argentina in 1988. Hybridization between species of the subgenus Culex has been demonstrated, hence the degree of reproductive isolation between Cx. bidens and Cx. declarator should be investigated, as well as their taxonomic status, because they only can be discriminated by a single male genitalic feature and not by nuclear or mitochondrial markers.Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Hoyos López, Richard. Universidad del Sinú; Colombi

Genetic diversity and phylogeny of Anopheles nuneztovari C and Anopheles albimanus, vectors of malaria in the department of Córdoba, Colombia

In the department of Córdoba, 21 widely distributed Anopheles species have been described, of which Anopheles triannulatus, Anopheles nuneztovari, Anopheles albimanus, and Anopheles darlingi are the most abundant species, and the last three play a vectorial role in Malaria transmission in Colombia. A correct taxonomic identification of malaria insect vectors is of vital importance for the development of effective vector control strategies. However, the identification of individuals from the Anopheles genus presents difficulties due to the loss of relevant morphological characters during the transport and preservation of the collected specimens. In addition to the interspecific variations, and the intraspecific similarities of the species belonging to the subgenus Nyssorhynchus, since itpresents species complexes and cryptic species that difficults identification based only in morphological characteristics. The objective of this study was to characterize the barcode fragment of the COI gene and its genetic diversity for the identification of An. (Nys) nuneztovari and An. (Nys) albimanus in areas of high malaria transmission from the department of Córdoba. 67 individuals belonging to the species An. nuneztovari cytotype C and 22 An. albimanus were identified, and additionally, 9 haplotypes were obtained for An. nuneztovari C and 14 for An. albimanus distributed in the study areas. The values obtained in the FST and Nm estimators indicate a low or null genetic differentiation and a high gene flow between most of the studied populations because they share the most frequent haplotypes of these two species. The maximum likelihood trees for these species showed that the specimens from Córdoba belong to the same mitochondrial lineage as those previously reported from Antioquia, Choco, and Norte de Santander

Phlebotomine sandflies (Diptera: Psychodidae) in a sub-Andean forest from the Norte de Santander, Colombia

Background & objectives: The recognition of communities of arthropods with medical importance in natural systems constitutes an important step in the prediction of possible epidemic events and/or emergence of infectious diseases in the human population. This is due to anthropogenic impact in natural areas and landscape modification, which changes the dynamics of pathogenic agents, reservoirs, and vector insects. In this study, an inventory was compiled of species of the genus Lutzomyia present in sub-Andean forest from the confluence of the Pamplonita River basin. Methods: CDC-light and Shannon traps were used for collecting adult phlebotomine sandflies during the month of October 2013 in a sub-Andean forest from river basin Pamplonita. All specimens were identified using morphological keys. The epidemiological relevance of each species was reported using a literature review about natural infection or vector incrimination with Leishmania species or other pathogens microorganism. Results: A total of 2755 specimens belonging to eight species of the genus Lutzomyia were collected. Out of the eight species, seven belonged to the group verrucarum (Lutzomyia sp - townsendi series, L. ovallesi, L. spinicrassa, L. serrana, L. townsendi, L. nuneztovari and L. pia), while one belonged to the subgenus Helcocyrtomyia (L. hartmanni). A new registry of L. townsendi was observed for the Norte de Santander department. Interpretation & conclusion: The appreciable diversity of the verrucarum group observed in this area suggest further investigation on the biogeography and evolution of this group, and epidemiological risk for human populations around this area, as there are reports of Leishmania natural infection and favourable conditions for domestication of phlebotomines in rural towns

Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia

Saint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts. Humans are occasionally infected by the virus and are dead-end hosts. SLEV causes encephalitis in temperate regions, while in tropical regions of the Americas, several human cases and a wide biological diversity of SLEV-strains have been reported. The phylogenetic analysis of the envelope (E) protein genes indicated eight-genotypes of SLEV with geographic overlap. The present paper describes the genotyping of two SLEV viruses detected in mosquito-pools collected in northern Colombia (department of Cordoba). We used reverse transcription-polymerase chain reaction to amplify a fragment of theE-gene to confirm the virus identity and completeE-gene sequencing for phylogenetic analysis and genotyping of the two-SLEV viruses found circulating in Córdoba. This is the first report of SLEV genotype IV in Colombia (Córdoba) in mosquitoes from a region of human inhabitation, implicating the risk of human disease due to SLEV infection. Physicians should consider SLEV as a possible aetiology for undiagnosed febrile and neurologic syndromes among their patients who report exposure to mosquito-bites

First report of Culex flavivirus infection from Culex coronator (Diptera: Culicidae), Colombia

Abstract Background Flaviviruses are important pathogens for humans and animals (Dengue viruses, Yellow fever virus, Zika virus and West Nile virus). Culex flavivirus (CxFV) is an insect-specific virus of the genus Flavivirus, detected in a wide variety of mosquito species. Objective To detect Flavivirus in mosquitoes of a tropical region of the Colombian Caribbean. Methods In 2014, an entomological surveillance of arboviruses was conducted in the department of Cordoba area of the Caribbean, Colombia. A total of 8270 mosquitoes were captured as follow: Mansonia (n = 3271/39.5%), Culex (n = 2668/32.26%), Anopheles (n = 840/10.15%), Aedeomyia (n = 411/4.9%), Psorophora (n = 397/4.8%), Coquilletidia (n = 369/4.46%), Uranotaenia (n = 261/3.15%) and Aedes (n = 53/0.6%). All mosquito species were collected in dry tropical forest of the Caribbean area. Universal primers for NS5 gene (958 pb), RT-PCR for flavivirus and sequencing were used for molecular identification of viruses detected. Results Two pools belonging to Culex coronator were positive for flavivirus RNA sequence by RT-PCR. The sequences of the PCR amplicons, matched that of the Culex flaviviruses, CxFv COL PM_149 (GenBank: KR014201) and CxFv COL PM_212 (GenBank: KT307717). Phylogenetic analysis of the NS5 protein sequences of the Culex flaviviruses sequences with those of reference sequences available in GenBank indicated viruses of Genotype II, closely related to the Brazilian strain, BR_SJRP_01_ (GenBank: KT726939), from Culex sp. The alignment of Culex flavivirus sequences CxFv COL_ PM 212 and CxFv COL_ PM 149 with sequences of strains detected in different geographical regions grouped the strains in a Latin American clade reported in Brazil, Argentina and Mexico. Conclusions The present work illustrated that CxFV was circulating among vectors of human pathogenic arboviruses in Colombia, but the impact of CxFV on other flaviviruses which are endemic in the study area still remains to be explored