Synergistic Signaling from Extracellular Matrix–Growth Factor Complexes

Investigations on extracellular matrix (ECM) and growth factor (GF) complexes have revealed an underappreciated phenomenon: they can either negate GF activity or generate synergistic signals for cell function, in particular mitogenesis. ECM and pericellular matrix molecules were first recognized to complex with GFs and regulate GF activity by the seminal observations that basic fibroblast growth factor (bFGF or FGF-2) required binding to a cell-surface heparin sulfate proteoglycan and to its authentic cell-surface receptor for biological activity (Klagsbrun and Baird, 1991; Yayon et al., 1991). Subsequently, numerous ECM–GF interactions that modulate GF activity were discovered; we have reviewed many of these findings (Macri et al., 2007)

Logan Medallist 4. Large-Scale Impact and Earth History

The current record of large-scale impact on Earth consists of close to 200 impact structures and some 30 impact events recorded in the stratigraphic record, only some of which are related to known structures. It is a preservation sample of a much larger production population, with the impact rate on Earth being higher than that of the moon. This is due to the Earth’s larger physical and gravitational cross-sections, with respect to asteroidal and cometary bodies entering the inner solar system. While terrestrial impact structures have been studied as the only source of ground-truth data on impact as a planetary process, it is becoming increasingly acknowledged that large-scale impact has had its effects on the geologic history of the Earth, itself. As extremely high energy events, impacts redistribute, disrupt and reprocess target lithologies, resulting in topographic, structural and thermal anomalies in the upper crust. This has resulted in many impact structures being the source of natural resources, including some world-class examples, such as gold and uranium at Vredefort, South Africa, Ni–Cu–PGE sulphides at Sudbury, Canada and hydrocarbons from the Campeche Bank, Mexico. Large-scale impact also has the potential to disrupt the terrestrial biosphere. The most devastating known example is the evidence for the role of impact in the Cretaceous–Paleocene (K–Pg) mass extinction event and the formation of the Chicxulub structure, Mexico. It also likely had a role in other, less dramatic, climatic excursions, such as the Paleocene–Eocene–Thermal Maximum (PETM) event. The impact rate was much higher in early Earth history and, while based on reasoned speculation, it is argued that the early surface of the Hadean Earth was replete with massive impact melt pools, in place of the large multiring basins that formed on the lower gravity moon in the same time-period. These melt pools would differentiate to form more felsic upper lithologies and, thus, are a potential source for Hadean-aged zircons, without invoking more modern geodynamic scenarios. The Earth-moon system is unique in the inner solar system and currently the best working hypothesis for its origin is a planetary-scale impact with the proto-Earth, after core formation at ca. 4.43 Ga. Future large-scale impact is a low probability event but with high consequences and has the potential to create a natural disaster of proportions unequalled by other geologic processes and threaten the extended future of human civilization, itself.RÉSUMÉLe bilan actuel de traces de grands impacts sur la Terre se compose de près de 200 astroblèmes et d'une trentaine d’impacts enregistrés dans la stratigraphie, dont seulement certains sont liés à des astroblèmes connus. Il s'agit d'échantillons préservés sur une population d’événements beaucoup plus importante, le taux d'impact sur Terre étant supérieur à celui de la lune. Cela tient aux plus grandes sections transversales physiques et gravitationnelles de la Terre sur la trajectoire des astéroïdes et comètes qui pénètrent le système solaire interne. Alors que les astroblèmes terrestres ont été étudiés comme étant la seule source de données avérée d’impacts en tant que processus planétaire, de plus en plus on reconnaît que les grands impacts ont eu des effets sur l'histoire géologique de la Terre. À l’instar des événements d'énergie extrême, les impacts redistribuent, perturbent et remanient les lithologies impliquées, provoquant dans la croûte terrestre supérieure des anomalies topographiques, structurelles et thermiques. Il en a résulté de nombreux astroblèmes à l’origine de ressources naturelles, dont certains exemples de classe mondiale tels que l'or et l'uranium à Vredefort en Afrique du Sud, les sulfures de Ni–Cu–PGE à Sudbury au Canada, et les hydrocarbures du Banc de Campeche au Mexique. Les grands impacts peuvent également perturber la biosphère terrestre. L'exemple le plus dévastateur connu nous est donné des indices du rôle de l'impact dans l'extinction de masse au Crétacé–Paléogène (K–Pg) et la formation de la structure de Chicxulub, au Mexique. Il a également probablement joué un rôle dans d'autres événements climatiques extraordinaires moins dramatiques, comme le Maximum thermal du Paleocène–Eocène (PETM). Le taux d'impact était beaucoup plus élevé au début de l'histoire de la Terre et, tout en étant basé sur une spéculation raisonnée, on fait valoir que la surface précoce de la Terre à l’Hadéen était tapissée de grands bassins en fusion, au lieu de grands bassins à couronnes multiples tels ceux qui se sont formés à la même période sur la lune ayant une gravité inférieure. Ces bassins en fusion se seraient différenciées pour constituer des lithologies plus felsiques sur le dessus, devenant ainsi une source potentielle de zircons d’âge Hadéen, sans qu’il soit nécessaire d’invoquer des scénarios géodynamiques plus récents. Le système Terre-lune est unique dans le système solaire interne. Actuellement la meilleure hypothèse de travail pour son origine est un impact planétaire avec la proto-Terre, après la formation du noyau à env. 4,43 Ga. La probabilité d’un futur grand impact est faible mais comporte des conséquences capables d’engendrer un désastre naturel aux proportions inégalées comparé à d'autres processus géologiques, menaçant l'avenir de la civilisation humaine elle-même

The Role of the Primary Visual Cortex in Higher Level Vision

In the classical feed-forward, modular view of visual processing, the primary visual cortex (area V1) is a module that serves to extract local features such as edges and bars. Representation and recognition of objects are thought to be functions of higher extrastriate cortical areas. This paper presents neurophysiological data that show the later part of V1 neurons’ responses reflecting higher order perceptual computations related to Ullman’s (Cognition 1984;18:97–159) visual routines and Marr’s (Vision NJ: Freeman 1982) full primal sketch, 2Image D sketch and 3D model. Based on theoretical reasoning and the experimental evidence, we propose a possible reinterpretation of the functional role of V1. In this framework, because of V1 neurons’ precise encoding of orientation and spatial information, higher level perceptual computations and representations that involve high resolution details, fine geometry and spatial precision would necessarily involve V1 and be reflected in the later part of its neurons’ activities.Mathematic

The Formation of Fragments at Corotation in Isothermal Protoplanetary Disks

Numerical hydrodynamics simulations have established that disks which are evolved under the condition of local isothermality will fragment into small dense clumps due to gravitational instabilities when the Toomre stability parameter $Q$ is sufficiently low. Because fragmentation through disk instability has been suggested as a gas giant planet formation mechanism, it is important to understand the physics underlying this process as thoroughly as possible. In this paper, we offer analytic arguments for why, at low $Q$, fragments are most likely to form first at the corotation radii of growing spiral modes, and we support these arguments with results from 3D hydrodynamics simulations.Comment: 21 pages, 1 figur

Transforming Growth Factor-β Stimulates the Expression of Fibronectin by Human Keratinocytes

abstractTransforming growth factor beta (TGF-β)is a 25-kD protein which has regulatory activity over a variety of cell types. It is distinct form epidermal growth factor (EGF) and EGF analogs, and exerts its action via a distinct receptor. Its effect on proliferation or differentiation can be positive or negative depending on the cell type and the presence of other growth factors. it also modulates the expression of cellular products. TGF-β causes fibroblasts to increase their production of the extracellular matrix components, fibronectin and collagen. human keratinocytes (HK) are known to have TGF-βreceptors. We wished to study the effect of TGF-β on the production of extracellular matrix proteins by human keratinocytes in culture.Human keratinocytes were grown in serum-free defined medium (MCDB-153) to about 70% confluence. Following a 16-h incubation in medium lacking EGF and TGF-β, cells were incubated for 12h in medium containing varying concentrations of EGF and TGF-β. Cells were then labeled with 35S-methionine for 10 h in the same conditions. Labeled proteins from the medium were analyzed by SDS-PAGE and autoradiography.TGF-β at 10 ng/ml induced a sixfold increase in the secretion of fibronectin, as well as an unidentified 50-kD protein. Thrombospondin production was also increased, but not over a generalized twofold increase in the production of all other proteins. EGF, at 10 ng/ml, caused a smaller additive effect, TGF-β may be an important stimulator of extracellular matrix production by human keratinocytes

Casting of Gold Nanoparticles with High Aspect Ratios inside DNA Molds

DNA nanostructures provide a powerful platform for the programmable assembly of nanomaterials. Here this approach is extended to synthesize rod-like gold nanoparticles in a full DNA controlled manner. The approach is based on DNA molds containing elongated cavities. Gold is deposited inside the molds using a seeded-growth procedure. By carefully exploring the growth parameters it is shown that gold nanostructures with aspect ratios of up to 7 can be grown from single seeds. The highly anisotropic growth is in this case controlled only by the rather soft and porous DNA walls. The optimized seeded growth procedure provides a robust and simple routine to achieve continuous gold nanostructures using DNA templating