O papel do gestor e curador da informação nos novos comportamentos informacionais

A new profession emerges with the information behaviour trend. The information manager and curator brings a multidisciplinar it and transdisciplinarity as a mediator and coworker in this scenario. The aim of this article is to analyse the information manager and curator´s role and his/her relation with the new informational behaviour. Also, it is discussed his/her way as a mediator and as a coworker and his/her importance to work along with the public policies about digital competencies. A conceptual analysis of the subject of this study is developed through literature review in the fields of Information Science, Information Technology and Sociology. In conclusion, this study brings on the importance of the information manager and curator as a mediator and coworker in the information society.publishersversionpublishe

Determinação de cálcio e magnésio em goma purificada de Tamboril e seus aspectos reológicos / Determination of calcium and magnesium in purified Monkfish gum and its rheological aspects

O Brasil é um país com enorme biodiversidade, que é muito pouco explorada. A goma do tamboril (Enterolobium contortisiliquum) é obtida a partir da exsudação que ocorre no tronco da árvore. Desta goma obtém-se um polissacarídeo que apresenta alto potencial industrial devido às suas propriedades físico-químicas, como gelificação, emulsificação e capacidade adesiva. Tais características têm relação com a composição mineral do produto, uma vez que a reologia pode estar associada aos minerais que compõem determinada matriz. Diante do exposto, o objetivo deste estudo foi avaliar o teor de cálcio e magnésio na goma purificada de tamboril. As amostras foram abertas com ácido nítrico e ácido perclórico na proporção de 2:1. A análise dos minerais cálcio e magnésio foi determinada por espectrometria de absorção atômica de chama, método direto de chama ar-acetileno (MALAVOLTA et al., 1997). Na goma foram encontrados, em média, 164,53 mg /100g de cálcio e 39,86 mg /100g de magnésio. A viscosidade de soluções pode aumentar com o incremento da concentração de determinados íons, como por exemplo a adição de cálcio. Estas alterações, quando em soluções de hidróxido de cálcio, estão associadas à capacidade de reticulação destas matrizes. O módulo de elasticidade G′ também pode aumentar com o aumento da concentração de íons cálcio, sugerindo fortalecimento da estrutura dos géis. Por outro lado, o aumento na concentração de magnésio pode diminuir a viscosidade. Íons de magnésio podem atuar como um agente bloqueador da gelificação da pectina. Ou seja, a presença de cálcio e magnésio na goma de tamboril determinam algumas de suas propriedades reológicas, podendo também alterá-las na base onde esta goma for adicionada. A goma de Enterolobium contortisiliquum, apesar de pouco explorada, tem elevado potencial tecnológico. Além da capacidade de alterar viscosidade e elasticidade, esta possui características nutricionais interessantes, além da potencial capacidade de produzir nanoesferas carreadoras de fármacos

Caracterização física e textural de biscoitos de farinha de banana verde / Physical and textural characterization of green banana flour biscuits

A utilização de farinha de banana verde (FBV) como ingrediente funcional para enriquecimento de produtos de panificação é bastante promissor. Nesse contexto, o objetivo desse estudo foi avaliar a influência da adição da FBV nas propriedades tecnológicas de biscoitos. Foi realizada uma breve caracterização da FBV (umidade, granulometria e capacidade de absorção de água) e avaliado as propriedades físicas e de textura dos biscoitos com duas concentrações de FBV (7,5 e 15%). A FBV obteve umidade de 12,02%. O perfil granulométrico mostrou que 59,86% da FBV apresentou granulometria inferior a 0,090 mm. As análises físicas mostraram que o peso e o volume específico dos biscoitos não foram afetados (p>0,05) com a adição da FBV apresentando peso de 20,12% ± 0,51 e volume específico de 2,45 mL/g ± 0,10. A textura dos biscoitos também não foi afetada significativamente (p>0,05), apresentando dureza de 7947,49 g ± 529,45 e fraturabilidade de 0,64 mm ± 0,085. A incorporação de FBV nas proporções de 7,5% e 15% em biscoitos apresentaram características físicas e texturais similares a formulação padrão mostrando ser uma alternativa interessante para melhorar a qualidade nutricional dos produtos

Rižine (Oryza sativa) mekinje i sojina (Glycine max) sačma kao nekonvencionalni dodaci u proizvodnji medovine

Research background. Due to the lack of nitrogen in honey, fermentation of honey must is limited or delayed, in addition to stimulating the production of unpleasant sensory compounds, such as sulfur derivatives. The use of natural supplements has been investigated as low-cost alternatives mainly to correct the nutritional deficiency of nitrogen in honey must in mead production. Experimental approach. Initially, the physicochemical characterization of the rice bran and soybean meal extracts was carried out. The fermentation of three yeasts (Saccharomyces bayanus Premier Blanc, Saccharomyces cerevisiae Montrachet and Saccharomyces cerevisiae Safbrew T-58) in honey must supplemented with 30 g/L rice bran or soybean meal extracts was evaluated. The trials were compared with the fermentations of the must with commercial supplement (30 g/L) and the control trials. Fermentations were carried out in Erlenmeyer flasks containing honey must with supplements, inoculated with 106 cell/mL yeast and incubated at 30 °C for 264 h. Results and conclusions. There was significant difference in the evaluated properties of the extracts, with the exception of reducing sugars. The fermentations with soybean meal extract reached the highest cell concentrations, as well as the largest consumption of glucose, fructose and ethanol. The glycerol concentrations slightly increased when soybean meal extract and commercial supplement were used. The highest concentrations of succinic and acetic acids were registered in the control trials produced by Saccharomyces strains Premier Blanc, Montrachet and Safbrew T-58. Formic and lactic acids were not produced. Results showed that the extracts can be used as low-cost alternatives for correcting the nutritional deficiency of nitrogen in honey must since their effect was similar to that of synthetic supplement. Novelty and scientific contribution. The use of low-cost, unconventional supplements such as those used in this work, in addition to reducing the cost of the process by reducing fermentation time and providing nutrients needed to improve yeast metabolism, prevents the formation of undesirable compounds in the beverage due to prolonged fermentation time. It also makes it possible to add value to industrial by-products. Unconventional supplements have still been little tested in mead production.Pozadina istraživanja. Vrenje meda ograničava ili usporava nedostatak dušika, koji uz to pospješuje nastanak spojeva nepoželjnih senzorskih svojstava, kao npr. derivata sumpora. U radu je ispitana primjena prirodnih dodataka kao jeftinih nadomjestaka koji korigiraju nedostatak dušika u moštu tijekom proizvodnje medovine. Eksperimentalni pristup. Prvo su opisana fizikalno-kemijska svojstva ekstrakata rižinih mekinja i sojine sačme. Zatim je ispitano vrenje meda s pomoću triju sojeva kvasaca (Saccharo¬myces bayanus Premier Blanc, Saccharomyces cerevisiae Montrachet i Saccharomyces cerevisiae Safbrew T-58) u moštu obogaćenom s 30 g/L ekstrakta rižinih mekinja ili sojine sačme. Rezultati tih eksperimenata uspoređeni su s rezultatima dobivenim vrenjem mošta s dodatkom 30 g/L komercijalnog suplementa, te s kontrolnim uzorcima. Vrenje mošta inokuliranog s 106 stanica kvasca po mL meda provedeno je u Erlenmeyerovim tikvicama inkubiranima na 30 °C tijekom 264 h. Rezultati i zaključci. Opažene su bitne razlike u svojstvima ekstrakata, osim u masenom udjelu reducirajućih šećera. Najviše stanica kvasaca dobiveno je vrenjem meda s dodatkom ekstrakta sojine sačme, pri čemu su kvasci potrošili najviše glukoze i fruktoze, te je dobiven najveći volumni udjel etanola. Koncentracije glicerola neznatno su se povećale pri dodatku sojine sačme i komercijalnog ekstrakta. Najveće su koncentracije jantarne i octene kiseline proizveli sojevi kvasaca Saccharomyces Premier Blanc, Montrachet i Safbrew T-58 u kontrolnim uzorcima. Pritom nije došlo do nastanka mravlje i mliječne kiseline. Rezultati pokazuju da se dobiveni ekstrakti mogu upotrijebiti za korekciju nedostatka dušika u moštu od meda, budući da su postignuti slični rezultati kao sa sintetičkim spojevima. Novina i znanstveni doprinos. Dodatkom ovakvih jeftinih nekonvencionalnih ekstrakata smanjuju se troškovi proizvodnje jer fermentacija kraće traje, a kvasci imaju dovoljno hranjiva, čime se sprječava nastanak nepoželjnih spojeva koji nastaju tijekom duljih fermentacija. Osim toga, korišteni industrijski nusproizvodi dobili su dodanu vrijednost. Primjena nekonvencionalnih suplemenata u proizvodnji medovine još nije dovoljno ispitana

Determination of harpagoside in Harpagophytum procumbens DC tablet’s using analytical method by High Performance Liquid Chromatography

The species Harpagophytum procumbens DC. is popularly known as devil’s claw and is widely used because of its anti-inflammatory properties that are attributed to an iridoid glycoside, the harpagoside. This medicinal plant is part of the Brazilian List of Essential Medicines, that is, it was selected and standardized for the resolution of diseases in the Brazilian public health system, SUS, and can be found in several brands. Due to the importance of quality monitoring, the objective of this study was to create and validate a method for identification and quantification of harpagoside in tablets of the devil’s claw, as well as to evaluate the content of this iridoid glycoside in three commercially available brands in Brasília, DF, Brazil. The evaluated parameters in the validation by high performance liquid chromatography (HPLC) were linearity, limits of detection and quantification, precision, selectivity, accuracy and robustness, as required by the Brazilian regulation. The developed and validated method proved satisfactory for the quality control of commercial products that present in this composition the medicinal plant. All the devil’s claw brands presented divergences regarding the quality that can interfere with therapeutics and with safety and efficacy

Innovations and developments in single cell protein: Bibliometric review and patents analysis

BackgroundGlobal demand for food products derived from alternative proteins and produced through sustainable technological routes is increasing. Evaluation of research progress, main trends and developments in the field are valuable to identify evolutionary nuances.MethodsIn this study, a bibliometric analysis and search of patents on alternative proteins from fermentation processes was carried out using the Web of Science and Derwent World Patents Index™ databases, using the keywords and Boolean operators “fermentation” AND “single cell protein” OR “single-cell protein.” The dataset was processed and graphics generated using the bibliometric software VOSviewer and OriginPro 8.1.ResultsThe analysis performed recovered a total of 360 articles, of which 271 were research articles, 49 literature review articles and 40 publications distributed in different categories, such as reprint, proceedings paper, meeting abstract among others. In addition, 397 patents related to the field were identified, with China being the country with the largest number of publications and patents deposits. While this topic is largely interdisciplinary, the majority of work is in the area of Biotechnology Applied Microbiology, which boasts the largest number of publications. The area with the most patent filings is the food sector, with particular emphasis on the fields of biochemistry, beverages, microbiology, enzymology and genetic engineering. Among these patents, 110 are active, with industries or companies being the largest depositors. Keyword analysis revealed that the area of study involving single cell protein has included investigation into types of microorganisms, fermentation, and substrates (showing a strong trend in the use of agro-industrial by-products) as well as optimization of production processes.ConclusionThis bibliometric analysis provided important information, challenges, and trends on this relevant subject

Síndrome de Hutchinson: Hutchinson syndrome

Introdução: A doença de Huntington (DH) é uma doença neurodegenerativa autossômica dominante que ocorre devido a mutação no gene da huntingtina, em seu terminal 5 ́, levando a uma expansão instável do tripleto de bases nitrogenadas CAG (citosina-adenina-guanina). Sua transmissão é autossômica dominante. Apresentação do caso Paciente do sexo feminino, com idade de 1 ano 5 meses, deu entrada no serviço ambulatorial de pediatria para acompanhamento devido atraso do crescimento e baixo peso corporal para faixa etária. Nascida com 37 semanas, de parto normal, a termo, com peso 3000 gramas, estatura de 51 cm, perímetro cefálico 34cm, APGAR 7. Discussão: a doença de huntington (DH) foi descrita no século 19 por George Huntington, o qual identificou características clínicas da doença e sua natureza hereditária. No entanto, foi apenas em 1993 que conseguiu isolar o gene e descobrir que a mutação responsável pela doença é a expansão da repetição dos trinucleotídeos CAG(citosina- adenina- guanina) localizada na região 5 do gene IT15 no braço curto do cromossomo 4.  O gene IT15 é responsável pela codificação da proteína Huntingtina, que está presente em vários tecidos do corpo, principalmente no cérebro. Quando no tecido cerebral, ela é quase exclusiva do citoplasma neural, sendo vista nos axônios, dendritos e corpo celular. Conclusão: é possível compreender a importância do manejo da Síndrome de Hutchinson desde sua apresentação até o tratamento de complicações visto que sua cura ainda está em investigação científica

Hepatoprotective effects of four Brazilian savanna species on acetaminophen-induced hepatotoxicity in HepG2 cells

We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts’ hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics.Faculdade de Ciências da Saúde (FS)Departamento de Farmácia (FS FAR)Instituto de Ciências Biológicas (IB)Departamento de Botânica (IB BOT)Programa de Pós-Graduação em Ciências Farmacêutica

Imatinib mesylate ameliorates the dystrophic phenotype in exercised mdx mice

Myofiber degeneration, inflammation, and fibrosis are remarkable features of Duchenne muscular dystrophy. We hypothesized that the administration of imatinib mesylate, an inhibitor of tyrosine kinase and TGF-beta pro-fibrogenic activity, could improve the muscular conditions in mdx mice. Four-week old mdx mice were treated and exercised for 6 weeks. Gastrocnemius and diaphragm histopathology, strength, creatine kinase, and cytokine levels were evaluated. The treated group presented increased muscular strength and decreased CK levels, injured myofibers, and inflammatory infiltrates. Pro-inflammatory cytokines and TGF-beta were also reduced, while IL-10 was increased, suggesting an immunomodulatory effect of imatinib, which can ameliorate the dystrophic phenotype in mdx mice. (C) 2009 Elsevier B.V. All rights reserved.PetrobrasFAPESPAADMUniversity of Ribeirao Preto-UNAER