Reversal-free CaIIH profiles: a challenge for solar chromosphere modeling in quiet inter-network

We study chromospheric emission to understand the temperature stratification in the solar chromosphere. We observed the intensity profile of the CaIIH line in a quiet Sun region close to the disk center at the German Vacuum Tower Telescope. We analyze over 10^5 line profiles from inter-network regions. For comparison with the observed profiles, we synthesize spectra for a variety of model atmospheres with a non local thermodynamic equilibrium (NLTE) radiative transfer code. A fraction of about 25% of the observed CaIIH line profiles do not show a measurable emission peak in H_{2v} and H_{2r} wavelength bands (reversal-free). All of the chosen model atmospheres with a temperature rise fail to reproduce such profiles. On the other hand, the synthetic calcium profile of a model atmosphere that has a monotonic decline of the temperature with height shows a reversal-free profile that has much lower intensities than any observed line profile. The observed reversal-free profiles indicate the existence of cool patches in the interior of chromospheric network cells, at least for short time intervals. Our finding is not only in conflict with a full-time hot chromosphere, but also with a very cool chromosphere as found in some dynamic simulations.Comment: 8 pages, accepted in A&

Dual pillars of hotel restaurant food quality satisfaction and brand loyalty

Purpose: The purpose of this paper is to investigate the impact of service quality (physical quality and staff behaviour) and brand equity (brand quality, brand awareness and brand image) on hotel restaurant food quality satisfaction and brand loyalty among international and local travellers in Malaysia. Design/methodology/approach: A total of 354 valid questionnaires were collected to assess the measurement and structural model for reflective latent constructs using the two steps of covariance-based structural equation modelling. Findings: The research findings suggest that while travellers’ nationality moderates the path between physical quality, staff behaviour and brand image on food quality satisfaction, it does not moderate the relationship between brand quality and brand awareness on food quality satisfaction. This finding further suggests that towards enhancing service quality and brand equity on food quality satisfaction and brand loyalty, managers should understand the important distinctions between international and local travellers. Originality/value: Previous studies have mostly examined the impact of brand equity and service quality towards tourist satisfaction and very few studies have examined the impact of restaurant service quality and brand equity on consumer satisfaction and brand loyalty specifically among international and local travellers. This is one of the first few studies providing empirical evidence and discussions in this area

High-resolution imaging and near-infrared spectroscopy of penumbral decay

Combining high-resolution spectropolarimetric and imaging data is key to understanding the decay process of sunspots as it allows us scrutinizing the velocity and magnetic fields of sunspots and their surroundings. Active region NOAA 12597 was observed on 24/09/2016 with the 1.5-m GREGOR solar telescope using high-spatial resolution imaging as well as imaging spectroscopy and near-infrared (NIR) spectropolarimetry. Horizontal proper motions were estimated with LCT, whereas LOS velocities were computed with spectral line fitting methods. The magnetic field properties were inferred with the SIR code for the Si I and Ca I NIR lines. At the time of the GREGOR observations, the leading sunspot had two light-bridges indicating the onset of its decay. One of the light-bridges disappeared, and an elongated, dark umbral core at its edge appeared in a decaying penumbral sector facing the newly emerging flux. The flow and magnetic field properties of this penumbral sector exhibited weak Evershed flow, moat flow, and horizontal magnetic field. The penumbral gap adjacent to the elongated umbral core and the penumbra in that penumbral sector displayed LOS velocities similar to granulation. The separating polarities of a new flux system interacted with the leading and central part of the already established active region. As a consequence, the leading spot rotated 55-degree in clockwise direction over 12 hours. In the high-resolution observations of a decaying sunspot, the penumbral filaments facing flux emergence site contained a darkened area resembling an umbral core filled with umbral dots. This umbral core had velocity and magnetic field properties similar to the sunspot umbra. This implies that the horizontal magnetic fields in the decaying penumbra became vertical as observed in flare-induced rapid penumbral decay, but on a very different time-scale.Comment: 14 pages, 11 figures, Accepted to be published in Astronomy and Astrophysic

Study of cosolvent-induced α-chymotrypsin fibrillogenesis: Does protein surface hydrophobicity trigger early stages of aggregation reaction?

The misfolding of specific proteins is often associated with their assembly into fibrillar aggregates, commonly termed amyloid fibrils. Despite the many efforts expended to characterize amyloid formation in vitro, there is no deep knowledge about the environment (in which aggregation occurs) as well as mechanism of this type of protein aggregation. Alpha-chymotrypsin was recently driven toward amyloid aggregation by the addition of intermediate concentrations of trifluoroethanol. In the present study, approaches such as turbidimetric, thermodynamic, intrinsic fluorescence and quenching studies as well as chemical modification have been successfully used to elucidate the underlying role of hydrophobic interactions (involved in early stages of amyloid formation) in α-chymotrypsin-based experimental system. © 2009 Springer Science+Business Media, LLC

Electrochemical-based biosensors for microRNA detection: Nanotechnology comes into view

Nanotechnology plays an undeniable significant role in medical sciences, particularly in the field of biomedicine. Development of several diagnostic procedures in medicine has been possible through the beneficial application of nano-materials, among which electrochemical nano-biosensors can be mentioned. They can be employed to quantify various clinical biomarkers in detection, evaluation, and follow up stages of the illnesses. MicroRNAs, a group of regulatory short RNA fragments, added a new dimension to the management and diagnosis of several diseases. Mature miRNAs are single-stranded RNA molecules approximately 22 nucleotides in length, which regulate a vast range of biological functions from cellular proliferation and death to cancer development and progression. Recently, diagnostic value of miRNAs in various diseases has been demonstrated. There are many traditional methods for detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR), microarray technology, nanotechnology-based approaches, and molecular biology tools including miRNA biosensors. In comparison with other techniques, electrochemical nucleic acid biosensor methods exhibit many interesting features, and could play an important role in the future nucleic acid analysis. This review paper provides an overview of some different types of nanotechnology-based biosensors for detection of miRNAs. © 201