Investigation of fruition rate and satisfaction of population from Baghodrat health center (Population research bare 2008)

Introduction: Health system services are an important program and successful from it’s in our country. Those principles are based on sameness. Social justice and community participation Fruition and satisfaction of population are an important aspect PHC (Primary health care). Objective: This study was done to evaluate fruition rat and satisfaction of population in the community research center of Kerman. Materials and Methods: This study is cross sectional and from kind of community Based participatory research. Views 328 person’s with method of cluster was c collected and data analysis was done. Results: 32.9 Percent had no file. Average 69.9 percent used not from services and only 17.7 percent used regularly and 13.8 percent irregularly. 23.8 percent were unsatisfactory. Among of 7 services had best of situation vaccination that 47% percent of population used regularly and 94.5 were unsatisfactory opposite 17% percent used from dentist services and 34.5 percent were un satisfaction. Conclusion: Census of region and to inform of population and supervise by responsible from this center is necessary that use from potentiality better Keywords: Satisfaction, Fruition, Health services, PRB

Electrochemical sensor for determination of hydroxylamine using functionalized Fe3O4nanoparticles and graphene oxide modified screen-printed electrode

A simple strategy for determination of hydroxylamine based on Fe3O4nanoparticles function-nalized by [2-(4-((3-(trimethoxysilyl)propylthio)methyl)1-H1,2,3-triazol-1-yl)aceticacid] (FNPs) and graphene oxide (GO) modified screen-printed electrode (SPE), denoted as (Fe3O4FNPs/GO/SPE), is reported. The electrochemical behavior of hydroxylamine was investigated at Fe3O4FNPs/GO/SPE by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chro-noamperometry (CHA) techniques in phosphate buffer solution (pH 7.0). Fe3O4FNPs/GO/SPE as a novel electrochemical sensor exhibited catalytic activity toward the oxidation of hydroxylami-ne. The potential of hydroxylamine oxidation was shifted to more negative potentials, and its oxidation peak current increased on the modified electrode, also indicatingthat under these conditions,the electrochemical process is irreversible. The electrocatalytic current of hydroxyl-amine showed a good relationship in the concentration range of 0.05–700.0 μM, with a detection limit of 10.0 nM. The proposed electrode was applied for the determination of hydroxylamine in water samples, too

Voltammetric folic acid sensor based on nickel ferrite nanoparticles modified-screen printed graphite electrode

In this study, an electrochemical sensor for the quantification of folic acid with voltam­metric detection in physiological conditions was constructed. For this purpose, nickel ferrite (NiFe2O4) nanoparticles were used to modify the surface of a screen-printed graphite electrode (NiFe2O4/SPGE) and applied in the determination of folic acid. The modified electrode displays a strong electrochemical response to folic acid. Folic acid was determined electrochemically using the differential pulse voltammetry (DPV) technique with a detection limit of 0.09±0.001 µM in 0.2–147.0 µM linear range in phosphate buffer solution (PBS) at pH 7.0 with this NiFe2O4/SPGE sensor, which has the best electron transfer rate. Also, the sensitivity of the modified electrode was obtained as 0.1139 µA µM-1. The NiFe2O4/SPGE sensor was successfully applied for the determination of folic acid in real samples

A novel dopamine electrochemical sensor based on La3+/ZnO nanoflower modified graphite screen printed electrode

Flower-like La3+/ZnO nanocomposite was facile synthesized. A simple and ultrasensitive sensor based on graphite screen printed electrode (SPE) modified by La3+/ZnO nanoflower was developed for the electrochemical determination of dopamine. The electrochemical behavior of dopamine was studied in 0.1 M phosphate buffer solution (PBS) using cyclic voltammetry (CV), chronoamperometry (CA) and differential pulse voltammetry (DPV). Compared with the unmodified graphite screen printed electrode, the modified electrode facilitates the electron transfer of dopamine, since it notably increases the oxidation peak current of dopamine. Also, according to CV results the maximum oxidation of dopamine on La3+/ZnO/SPE occurs at 150 mV which is about 140 mV more negative compared with unmodified SPE. Under optimized conditions, the modified electrode exhibited a linear response over the concentration range from 0.15 to 300.0 μM, with a detection limit of 0.08 μM (S/N = 3). The proposed sensor exhibited a high sensitivity, good stability and was successfully applied for dopamine determination in dopamine ampoule, with high recovery

The effects of hormonal changes on sperm DNA integrity in oligoasthenoteratospermia individuals: A case-control study

Background: Hormonal imbalance is one of the important etiological factors for Oligoasthenoteratospermias (OAT). Objective: This study aimed to evaluate the effects of hormonal changes including prolactin, TSH, testosterone, luteinizing hormone, follicle-stimulating hormone, and anti-Mullerian hormone on sperm DNA fragmentation in normal men compared with OAT to design a clinical algorithm for the comprehensive study of male factor infertilities. Materials and Methods: We consecutively selected 60 candidates referred to the infertility clinic to collect the semen and blood samples. Then, a terminal deoxynucleotidyl transferase dUTP nick end labeling test was performed to evaluate the sperm DNA fragmentation index (DFI). After semen analysis and DFI checking, they were classified into 4 groups consisting of normospermia and OAT men each with or without increased DFI. Hormone parameters were analyzed using enzyme-linked immunoassay. Results: Follicle-stimulating hormone and luteinizing hormone levels showed positive correlations with DFI in a significant way (p ≤ 0.01), while testosterone and thyroidstimulating hormone were associated with sperm concentration. Prolactin and anti- Mullerian hormone levels significantly correlated (p ≤ 0.01) with sperm concentration and DFI value simultaneously. Conclusion: Decreased and increased levels of serum hormones could adversely affect semen profile and sperm DNA integrity which lead to severe male infertility. Although we investigated the effects of the main hormones related to male infertility on DNA damage, the role of these hormones on the fertilization rate and embryo quality needs to be evaluated in further studies. Key words: DNA fragmentation, Oligospermia, Asthenospermia, Teratospermia, Hormones

Changes in the pattern of opium addiction in Bam after the earthquake

Introduction: Opium abuse is a serious public health threat of various magnitudes in Iran and neighboring countries. We conducted this study to explore the rate and intensity of opium addiction in Bam before the earthquake in comparison to the data collected after the earthquake.Methods: This descriptive cross-sectional study was conducted in the city of Bam in 2010. We used cluster random sampling method to collect data. This was done through house-to-house visits by a team of trained indigenous health personnel and informants. The status of addiction was achieved through case informants and confirmed by an experienced physician.Results: Opium addiction was significantly higher in men aged more than 50 (46%), illiterates (29.8%), labors/farmers (51.3%), private (50.7%) and jobless (21.6%) participants. Majority of the addicts (17.1%) were from the suburban areas (17.1%) as compared with those in urban areas (13.6%, P Conclusion: It seems that earthquake is a major traumatic risk factor for such an epidemic condition compared to the previous state of opium addiction. The present findings could be used for future prophylactic measures and therapeutic control strategies. These findings can be applied in clinical practice for active detection of opioid cases and subsequent treatment

Characteristics of the human endometrial regeneration cells as a potential source for future stem cell-based therapies: A lab resources study

Background: Human endometrium with consecutive regeneration capability undergoes monthly hormonal changes for probable implantation, which confirms the presence of the cells in the basalis layer known as stem cell. Objective: Previously, we reported the isolation and culture of the mesenchymal-like cells from human endometrium. In this study, we evaluated the biological and stemness characteristics of these cells. Materials and Methods: The characterization of Yazd human endometrialderived mesenchymal stem/stromal cells (YhEnMSCs) was assessed using immunofluorescence (IF) staining for CD105, VIMENTIN, and FIBRONECTIN as markers and RT-PCR for CD166, CD10, CD105, VIMENTIN, FIBRONECTIN, MHCI, CD14, and MHCII genes. Flow cytometry (FACS) was performed for CD44, CD73, CD90, and CD105 markers. Moreover, the differentiation capacity of the YhEnMSCs to the osteoblast and adipocytes was confirmed by Alizarin Red and Oil Red staining. Results: YhEnMSCs expressed CD105, VIMENTIN, FIBRONECTIN, CD44, CD73, and CD90 markers and CD166, CD10, CD105, VIMENTIN, FIBRONECTIN, and MHCI, but, did not express CD14, MHCII. Conclusion: Our data confirm previous reports by other groups indicating the application of endometrial cells as an available source of MSCs with self-renewal and differentiation capacity. Accordingly, YhEnMSCs can be used as a suitable source for cell-based therapies. Key words: Cell-based therapy, Endometrium, Mesenchymal stem/stromal cells, Regenerative medicine, Stem cells, Uterus

Glutathione detection at carbon paste electrode modified with ethyl 2-(4-ferrocenyl-[1,2,3]triazol-1-yl)acetate, ZnFe2O4nano-particles and ionic liquid

The purpose of the present study was to introduce a newly designed approach for deter-mination of glutathione using modified carbon paste electrode with ZnFe2O4nanoparticles, ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) and ethyl-2-(4-ferrocenyl--[1,2,3]triazol-1-yl)acetate (EFTA/ZFO/IL/CPE). According to the results from the electro-chemical experiments, oxidation current of glutathione on the modified electrode surface was incremented and its oxidation potential was decreased compared to bare CPE. A linear response was observed for the electrode at different glutathioneconcentrations (0.2 to 300.0 μM)

Electrochemical measurements of ascorbic acid based on graphite screen printed electrode modified with La3+/Co3O4 nanocubes transducer

Electrochemical characterization of ascorbic acid oxidation on a graphite screen printed electrode (SPE) modified with La3+/Co3O4nanocubes is performed by applying cyclic voltammetry (CV), chronoamperometry (CHA), and differential pulse voltammetry (DPV) techniques. Synthesized La3+/Co3O4nanocubes for SPE modification, La3+/Co3O4/SPE, enhance the ascorbic acid electrooxidation kinetics by reducing the anodic overpotential. Excellent La3+/Co3O4/SPE electrochemical properties provide sensitive ascorbic acid voltammetric determination with low detection limit, good stability and quick response towards electrooxidation of ascorbic acid as compared to bare SPE. Under optimized conditions, DPV current demonstrates a linear response for ascorbic acid over a concentration range of 1.0 to 900.0 mM with a correlation coefficient of 0.9997, and detection limit (LOD) (S/N = 3) = 0.3 mM. The proposed procedure offers a potential application for producing the sensor with good repeatability. Also, fast response of fabricated sensor can allow a real-time analysis of real samples

Histopathologic evaluation of the inflammatory factors and stromal cells in the endometriosis lesions: A case-control study

Background: Endometriosis is a multifaceted gynecological disorder defined as a benign estrogen-dependent chronic inflammatory process in which endometrial glands and stroma-like tissues are located outside the uterine cavity. It affects around 2-10% of all women during their reproductive years. Objective: This study aimed to evaluate the traffic of mesenchymal stem cells and inflammatory factors toward the lesions. Materials and Methods: Ten samples of normal endometrium and eutopic endometrium were studied as a control group and 10 ectopic samples were considered as a case group. Hematoxylin and eosin staining was used to evaluate stromal cells and inflammatory cells. Immunohistochemical staining was performed to show the presence of proliferating cell nuclear antigen in the lesions. The cells were digested and cultured in the laboratory to study cell proliferation. The number of cells and vessels were counted with Image J software, and data analysis was performed with Prism software. Results: Data analysis showed that the number of stromal cells and vessels in ectopic tissue were significantly higher than the control group (p < 0.001). Also, the number of inflammatory cells, including neutrophils, monocytes, lymphocytes, and macrophages, in the ectopic group was much higher than in the control group (p < 0.005). Conclusion: By expanding the number of blood vessels, blood flow increases, and cell migration to tissues is facilitated. The accumulation of inflammatory cells, especially macrophages, stimulates the growth of stem cells and helps implant cells by creating an inflammatory process. Key words: Endometriosis, PCNA, Stem cell, Inflammation