The impact of supplemental L-threonine in laying hen diets on egg component yield, composition, and functionality

The impacts of supplemental L-threonine in laying hen diets were evaluated. Over three experiments, control hens were fed a corn-soybean commercial layer diet containing 0.56% threonine (Thr). Experimental diets containing 0.66, 0.76, 0.86, and 0.96% Thr were fed for experiment 1. Experimental diets containing 0.76, 0.96 and 1.16% Thr were fed for experiment 2. Experiment 1 and 2 hens were 42 weeks of age. In experiment 3, experimental diets containing 0.76 and 0.96% Thr were fed to aged hens (61 weeks at beginning of experiment). Data collection methods were the same for all three experiments. Beginning and ending hen weight, egg production, and feed consumption data were collected. Egg samples were analyzed for egg weight, yolk and albumen yield, protein, and functionality. In experiments 1 and 2, egg production increased with increasing dietary threonine levels up to 0.76% Thr in the diet and subsequently decreased suggesting a production threshold for the amino acid. Shell cracking strength increased with increasing threonine levels in all three experiments. In experiment 3, shell thickness increased with increasing threonine levels. Albumen protein was significantly increased when hens were fed increased levels of dietary threonine. Angel food cake volume was significantly increased in experiments 1 and 3 with increasing dietary threonine, as were other texture profile parameters. Sponge cake volume was significantly increased in experiments 2 and 3 as a result of increased threonine levels. In experiment 3, yolk gel hardness was significantly increased by increasing the level of dietary threonine. These data clearly indicate a potential important impact on egg composition and functionality by increasing dietary threonine nutrition of a laying hen

Reforming Ritual: Protestantism, Women, and Ritual on the Renaissance Stage

My dissertation focuses on representations of women and ritual on the Renaissance stage, situating such examples within the context of the Protestant Reformation. The renegotiation of the value, place, and power of ritual is a central characteristic of the Protestant Reformation in early modern England. The effort to eliminate or redirect ritual was a crucial point of interest for reformers, for most of whom the corruption of religion seemed bound to its ostentatious and idolatrous outer trappings. Despite the opinions of theologians, however, receptivity toward the structure, routine, and familiarity of traditional Catholicism did not disappear with the advent of Protestantism. Reformers worked to modify those rituals that were especially difficult to eradicate, maintaining some sense of meaning without portraying confidence in ceremony itself. I am interested in how early Protestantism dealt with the presence of elements (in worship, daily practice, literary or dramatic representation) that it derogatorily dubbed popish, and how women had a particular place of importance in this dialogue. Through the drama of Shakespeare, Webster, and Middleton, along with contemporary religious and popular sources, I explore how theatrical representations of ritual involving women create specific sites of cultural and theological negotiation. These representations both reflect and resist emerging attitudes toward women and ritual fashioned by Reformation thought, granting women a particular authority in the spiritual realm

Tailored Porous Carbons Enabled by Persistent Micelles With Glassy Cores

Porous nanoscale carbonaceous materials are widely employed for catalysis, separations, and electrochemical devices where device performance often relies upon specific and well-defined regular feature sizes. The use of block polymers as templates has enabled affordable and scalable production of diverse porous carbons. However, popular carbon preparations use equilibrating micelles which can change dimensions in response to the processing environment. Thus, polymer methods have not yet demonstrated carbon nanomaterials with constant average template diameter and tailored wall thickness. In contrast, persistent micelle templates (PMTs) use kinetic control to preserve constant micelle template diameters, and thus PMT has enabled constant pore diameter metrics. With PMT, the wall thickness is independently adjustable via the amount of material precursor added to the micelle templates. Previous PMT demonstrations relied upon thermodynamic barriers to inhibit chain exchange while in solution, followed by rapid evaporation and cross-linking of material precursors to mitigate micelle reorganization once the solvent evaporated. It is shown here that this approach, however, fails to deliver kinetic micelle control when used with slowly cross-linking material precursors such as those for porous carbons. A new modality for kinetic control over micelle templates, glassy-PMTs, is shown using an immobilized glassy micelle core composed of polystyrene (PS). Although PS based polymers have been used to template carbon materials before, all prior reports included plasticizers that prevented kinetic micelle control. Here the key synthetic conditions for carbon materials with glassy-PMT control are enumerated, including dependencies upon polymer block selection, block molecular mass, solvent selection, and micelle processing timeline. The use of glassy-PMTs also enables the direct observation of micelle cores by TEM which are shown to be commensurate with template dimensions. Glassy-PMTs are thus robust and insensitive to material processing kinetics, broadly enabling tailored nanomaterials with diverse chemistries

DNA methylation signature of passive smoke exposure is less pronounced than active smoking: the Understanding Society study

Introduction The extent of the biological impact of passive smoke exposure is unclear. We sought to investigate the association between passive smoke exposure and DNA methylation, which could serve as a biomarker of health risk. Materials and methods We derived passive smoke exposure from self-reported questionnaire data among smoking and non-smoking partners of participants enrolled in the UK Household Longitudinal Study ‘Understanding Society’ (n=769). We performed an epigenome-wide association study (EWAS) of passive smoke exposure with DNA methylation in peripheral blood measured using the Illumina Infinium Methylation EPIC array. Results No CpG sites surpassed the epigenome-wide significance threshold of p<5.97 × 10−8 in relation to partner smoking, compared with 10 CpG sites identified in relation to own smoking. However, 10 CpG sites surpassed a less stringent threshold of p<1 × 10−5 in a model of partner smoking adjusted for own smoking (model 1), 7 CpG sites in a model of partner smoking restricted to non-smokers (model 2) and 16 CpGs in a model restricted to regular smokers (model 3). In addition, there was evidence for an interaction between own smoking status and partners’ smoking status on DNA methylation levels at the majority of CpG sites identified in models 2 and 3. There was a clear lack of enrichment for previously identified smoking signals in the EWAS of passive smoke exposure compared with the EWAS of own smoking. Conclusion The DNA methylation signature associated with passive smoke exposure is much less pronounced than that of own smoking, with no positive findings for ‘expected’ signals. It is unlikely that changes to DNA methylation serve as an important mechanism underlying the health risks of passive smoke exposure

Introduction: Shakespeare's public spheres

Habermas’ sense of a “cultural Public Sphere” is a notoriously complex term and, when applied to Early Modern cultures, needs careful definition. This essay both introduces the variety of methods by which we might approach playtexts with a view to their public – auditory – impact and contributes to a debate about an audience's understanding of Shakespeare's plays. By selecting two words and their spread of use in one play, Twelfth Night, we might appreciate the potential for meaningful ambiguity latent in how we hear the language of live performance. If we search for how certain terms (in this case, the cluster of semes derived from repetitions of “fancy” and “play”), we might find at times incompatible senses, yet we get near to appreciating the range of Early Modern dramatic language

Mechanisms Promoting the Long-Term Persistence of a Wolbachia Infection in a Laboratory-Adapted Population of Drosophila melanogaster

Intracellular bacteria of the genus Wolbachia are widespread endosymbionts across diverse insect taxa. Despite this prevalence, our understanding of how Wolbachia persists within populations is not well understood. Cytoplasmic incompatibility (CI) appears to be an important phenotype maintaining Wolbachia in many insects, but it is believed to be too weak to maintain Wolbachia in Drosophila melanogaster, suggesting that Wolbachia must also have other effects on this species. Here we estimate the net selective effect of Wolbachia on its host in a laboratory-adapted population of D. melanogaster, to determine the mechanisms leading to its persistence in the laboratory environment. We found i) no significant effects of Wolbachia infection on female egg-to-adult survival or adult fitness, ii) no reduced juvenile survival in males, iii) substantial levels of CI, and iv) a vertical transmission rate of Wolbachia higher than 99%. The fitness of cured females was, however, severely reduced (a decline of 37%) due to CI in offspring. Taken together these findings indicate that Wolbachia is maintained in our laboratory environment due to a combination of a nearly perfect transmission rate and substantial CI. Our results show that there would be strong selection against females losing their infection and producing progeny free from Wolbachia