88 research outputs found
Techniques and Bio-Medical Applications of Field-Cycling Magnetic Resonance
Non peer reviewedPublisher PD
Species of a whale and an unknown fish sample identified using molecular taxonomy
Molecular genetics provides a powerful tool for conservation of species protected by international
regulations or threatened by overexploitation. The present communication is the first report from
India on the application of molecular tools for the accurate identification of a stranded whale in
putrefied condition as it was impossible to identify the species status using conventional taxonomy
and the carcass of an unknown animal devoid of its head and tail, collected from a fish market.
Partial sequences of mtDNA control region and cytochrome b gene of the whale were generated and
tested with BLAST search and DNA surveillance for molecular identification. It was identified as
Bryde's whale (Balaenoptera edeni). Partial sequence of mtDNA cytochrome b gene of the unknown
fish from the market was generated, tested with BLAST search and was identified as sword fish
Xiphias gladius
Field-Cycling Magnetic Resonance Imaging - a Curiosity or the Next Big Thing?
Non peer reviewedPublisher PD
Identification of microsatellite loci, gene ontology and functional gene annotations in Indian salmon (Eleutheronema tetradactylum) through nextgeneration sequencing technology using illumina platform
Whole genome sequencing was performed on three samples of four finger threadfin Eleutheronema tetradactylum
(KET25, KET29 and KET30) using illumina NextSeq500 platform using 2×150 bp chemistry. 8,390,317,
7,085,775 and 8,461,589 high quality reads were obtained after trimming low quality reads and adapter sequence.
These high quality reads obtained were used for de novo assembly and obtained a number of scaffolds.
From these scaffolds of vast sequenced data, we were able to identify 60246, 46107 and 60907 Simple Sequence
Repeats (SSR) markers in KET25, KET29 and KET30 respectively, which will be useful in population genetic
analysis and other diversity studies in Indian salmon. The gene prediction on assembled scaffolds predicted
31,943 genes for KET25; 26,487 genes for KET29 and 31,654 genes for KET30 with average gene size of 458bp,
424bp and 459bp respectively. A total of 30,209, 25,107 and 29,943 genes were annotated against the NCBI Nr
database for the samples respectively. E. tetradactylum is a commercially important fish species for many
countries. This is the first report on the identification of genomic SSR markers in E. tetradactylum using NGS
technology. This study provides an insight of baseline knowledge of the genome sequence of Indian salmon for
future studies
DNA barcoding and molecular taxonomy of marine organisms In: Winter School on Vistas in Marine Biotechnology 5th to 26th October 2010
Taxonomical awareness on species, the intraspecific and interspecific relations etc, are the
necessary pre requisites in different fields of applied biology, such as in biological resources
management and conservation, in mariculture ventures, in pest/pathogen control and identification,
etc. Generally, there are two contexts in which significant problems arise in species identification,
where the molecular approaches are applicable. The first concerns the very fundamentals of
taxonomy, in identifying the species, the phylogenetic status and the identification at subspecies
and hybrids level. The second concerns to ecologists are the issue of identifying the species sex, or
identity of individuals under circumstances where simple morphology cannot be relied upon.
Biodiversity studies require species level analyses to assess the community structures. In conventional
taxonomic practice, organisms were classified based on their fine morphological characters,
comparative osteology, etc., which are more time consuming, laborious and expensive. Moreover,
in some situations, morphological variation arises due to the environmental factors rather than genetic
causes and is therefore not heritable. In this context, classification of organisms based on the
molecular variations using the modern molecular tools has got a wide acceptance globally
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