Molecular Interactions Between Plants and Insect Herbivores.

Diverse molecular processes regulate the interactions between plants and insect herbivores. Here, we review genes and proteins that are involved in plant-herbivore interactions and discuss how their discovery has structured the current standard model of plant-herbivore interactions. Plants perceive damage-associated and, possibly, herbivore-associated molecular patterns via receptors that activate early signaling components such as Ca <sup>2+</sup> , reactive oxygen species, and MAP kinases. Specific defense reprogramming proceeds via signaling networks that include phytohormones, secondary metabolites, and transcription factors. Local and systemic regulation of toxins, defense proteins, physical barriers, and tolerance traits protect plants against herbivores. Herbivores counteract plant defenses through biochemical defense deactivation, effector-mediated suppression of defense signaling, and chemically controlled behavioral changes. The molecular basis of plant-herbivore interactions is now well established for model systems. Expanding molecular approaches to unexplored dimensions of plant-insect interactions should be a future priority

Anomalous double peak structure in Nb/Ni superconductor/ferromagnet tunneling DOS

We have experimentally investigated the density of states (DOS) in Nb/Ni (S/F) bilayers as a function of Ni thickness, $d_F$. Our thinnest samples show the usual DOS peak at $\pm\Delta_0$, whereas intermediate-thickness samples have an anomalous ``double-peak'' structure. For thicker samples ($d_F \geq 3.5$ nm), we see an ``inverted'' DOS which has previously only been reported in superconductor/weak-ferromagnet structures. We analyze the data using the self-consistent non-linear Usadel equation and find that we are able to quantitatively fit the features at $\pm\Delta_0$ if we include a large amount of spin-orbit scattering in the model. Interestingly, we are unable to reproduce the sub-gap structure through the addition of any parameter(s). Therefore, the observed anomalous sub-gap structure represents new physics beyond that contained in the present Usadel theory.Comment: 4 pages, 3 figure

Insect egg-induced innate immunity: Who benefits?

Plants perceive the presence of insect eggs deposited on leaves as a cue of imminent herbivore attack. Consequential plant signaling events include the accumulation of salicylic acid and reactive oxygen species, transcriptional reprogramming, and cell death. Interestingly, egg-induced innate immunity shows similarities with immune responses triggered upon recognition of microbial pathogens, and in recent years, it became apparent that egg perception affects plant-microbe interactions. Here, we highlight recent findings on insect egg-induced innate immunity and how egg-mediated signaling impacts plant-microbe interactions. Ecological considerations beg the question: Who benefits from egg perception in these complex interactions

Sphingolipids are involved in insect egg-induced cell death in Arabidopsis.

In Brassicaceae, hypersensitive-like programmed cell death (HR-like) is a central component of direct defenses triggered against eggs of the large white butterfly (Pieris brassicae). The signaling pathway leading to HR-like in Arabidopsis (Arabidopsis thaliana) is mainly dependent on salicylic acid (SA) accumulation, but downstream components are unclear. Here, we found that treatment with P. brassicae egg extract (EE) triggered changes in expression of sphingolipid metabolism genes in Arabidopsis and black mustard (Brassica nigra). Disruption of ceramide (Cer) synthase activity led to a significant decrease of EE-induced HR-like whereas SA signaling and reactive oxygen species levels were unchanged, suggesting that Cer are downstream activators of HR-like. Sphingolipid quantifications showed that Cer with C16:0 side chains accumulated in both plant species and this response was largely unchanged in the SA-induction deficient2 (sid2-1) mutant. Finally, we provide genetic evidence that the modification of fatty acyl chains of sphingolipids modulates HR-like. Altogether, these results show that sphingolipids play a key and specific role during insect egg-triggered HR-like

Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.

Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores

An Escherichia coli O157 : H7 outbreak?

© 2000 by the Infectious Diseases Society of America. All rights reserved

Trade-off between constitutive and inducible resistance against herbivores is only partially explained by gene expression and glucosinolate production.

The hypothesis that constitutive and inducible plant resistance against herbivores should trade-off because they use the same resources and impose costs to plant fitness has been postulated for a long time. Negative correlations between modes of deployment of resistance and defences have been observed across and within species in common garden experiments. It was therefore tested whether that pattern of resistance across genotypes follows a similar variation in patterns of gene expression and chemical defence production. Using the genetically tractable model Arabidopsis thaliana and different modes of induction, including the generalist herbivore Spodoptera littoralis, the specialist herbivore Pieris brassicae, and jasmonate application, constitutive and inducibility of resistance was measured across seven A. thaliana accessions that were previously selected based on constitutive levels of defence gene expression. According to theory, it was found that modes of resistance traded-off among accessions, particularly against S. littoralis, in which accessions investing in high constitutive resistance did not increase it substantially after attack and vice-versa. Accordingly, the average expression of eight genes involved in glucosinolate production negatively predicted larval growth across the seven accessions. Glucosinolate production and genes related to defence induction on healthy and herbivore-damaged plants were measured next. Surprisingly, only a partial correlation between glucosinolate production, gene expression, and the herbivore resistance results was found. These results suggest that the defence outcome of plants against herbivores goes beyond individual molecules or genes but stands on a complex network of interactions

Constraining the nuclear equation of state at subsaturation densities

Only one third of the nucleons in $^{208}$Pb occupy the saturation density area. Consequently nuclear observables related to average properties of nuclei, such as masses or radii, constrain the equation of state (EOS) not at saturation density but rather around the so-called crossing density, localised close to the mean value of the density of nuclei: $\rho\simeq$0.11 fm$^{-3}$. This provides an explanation for the empirical fact that several EOS quantities calculated with various functionals cross at a density significantly lower than the saturation one. The third derivative M of the energy at the crossing density is constrained by the giant monopole resonance (GMR) measurements in an isotopic chain rather than the incompressibility at saturation density. The GMR measurements provide M=1110 $\pm$ 70 MeV (6% uncertainty), whose extrapolation gives K$_\infty$=230 $\pm$ 40 MeV (17% uncertainty).Comment: 4 pages, 4 figure