1,377 research outputs found

    Las inmunoglobulinas Y, como una alternativa a la antibioterapia contra Escherichia coli en sistemas de producción de pollos Broilers.

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    En el cantón Lomas de Sargentillo provincia del Guayas, se realizó la presente investigación. Los objetivos fueron los siguientes: determinar la eficacia de las inmunoglobulinas Y, ante retos de E. coli en pollos broilers, analizar la inocuidad del uso de inmunoglobulinas en pollos broilers, realizar un análisis de costos de tratamientos con IgY vs Enrofloxacina. Se utilizó pollos broilers de 28 días, distribuidos en 5 grupos de 50 pollos. Se inoculó 1 dosis letal 50 % de E. coli O111: H, a todos los grupos y 48 horas post inoculación, se aplicó las IgY, al grupo 1 a las 48 horas, grupo 2 a las 48 y 72 horas, grupo 3 a las 48, 72 y 96 horas, al grupo 4 se aplicó enrofloxacina y el grupo 5 fue control. A través de la conversión alimenticia se evidencia que la eficacia de la IgY es nula de acuerdo a la ANOVA y a la correlación de Fisher, no hubo diferencia significativa entre los grupos que recibieron IgY y los que no recibieron, no se observó mortalidad y la morbilidad fue del 20 % durante los 13 días de estudio, el tratamiento con mayor cantidad de IgY, tuvo un costo de USD 6.00,comparadoconlaenrofloxacinaUSD 6.00, comparado con la enrofloxacina USD 31,54. Se concluye que las IgY no son eficaces contra E. coli inoculado vía S.C., son inocuas y los tratamientos empleándolas son más económicos que la administración de Enrofloxacina

    El discurso público del yihad y las modificaciones de la violencia política en Latinoamérica

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    Se diría que en América Latina han cambiado las formas de la violencia. No es que hayan desaparecido por completo las fórmulas convencionales –secuestro (express o clásico), robos, narcotráfico, conflictos internos…- sino que, o bien alguna de ellas se han modificado -tal y como se ha demostrado en tesis doctorales recientes (Patiño, 2015)- o bien han aparecido otras impredecibles para el ciudadano de a pie. Una de esas mutaciones es el peso que los extremismos parecen haber adquirido en la región, así como el influjo creciente del Islam y del islamismo en la derecha y en la izquierda, con un discurso del yihad que encuentra, contra todo pronóstico, acomodo. De una de estas transformaciones -de las obsesiones islámicas y yihadistas en grupos naturalmente alejados de estas doctrinas– trataremos en este trabajo

    Movement velocity can be used to estimate the relative load during the bench press and leg press exercises in older women

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    Background: Movement velocity has been proposed as an effective tool to prescribe the load during resistance training in young healthy adults. This study aimed to elucidate whether movement velocity could also be used to estimate the relative load (i.e., % of the one-repetition maximum (1RM)) in older women. Methods: A total of 22 older women (age = 68.2 ± 3.6 years, bench press 1RM = 22.3 ± 4.7 kg, leg press 1RM = 114.6 ± 15.9 kg) performed an incremental loading test during the free-weight bench press and the leg press exercises on two separate sessions. The mean velocity (MV) was collected with a linear position transducer. Results: A strong linear relationship between MV and the relative load was observed for the bench press (%1RM = -130.4 MV + 119.3; r2 = 0.827, standard error of the estimate (SEE) = 6.10%1RM, p < 0.001) and leg press exercises (%1RM = -158.3 MV + 131.4; r2 = 0.913, SEE = 5.63%1RM, p < 0.001). No significant differences were observed between the bench press and leg press exercises for the MV attained against light-medium relative loads (>70%1RM), while the MV associated with heavy loads (>80%1RM) was significantly higher for the leg press. Conclusions: These results suggest that the monitoring of MV could be useful to prescribe the loads during resistance training in older women. However, it should be noted that the MV associated with a given %1RM is significantly lower in older women compared to young healthy individuals

    Improving Cognitive Visual-Motor Abilities in Individuals with Down Syndrome

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    Down syndrome causes a reduction in cognitive abilities, with visual-motor skills being particularly affected. In this work, we have focused on this skill in order to stimulate better learning. The proposal relies on stimulating the cognitive visual-motor skills of individuals with Down Syndrome (DS) using exercises with a gestural interaction platform based on the KINECT sensor named TANGO:H, the goal being to improve them. To validate the proposal, an experimental single-case study method was designed using two groups: a control group and an experimental one, with similar cognitive ages. Didactic exercises were provided to the experimental group using visual cognitive stimulation. These exercises were created on the TANGO:H Designer, a platform that was designed for gestural interaction using the KINECT sensor. As a result, TANGO:H allows for visual-motor cognitive stimulation through the movement of hands, arms, feet and head. The “Illinois Test of Psycholinguistic Abilities (ITPA)” was applied to both groups as a pre-test and post-test in its four reference sections: visual comprehension, visual-motor sequential memory, visual association, and visual integration. Two checks were made, one using the longitudinal comparison of the pre-test/post-test of the experimental group, and another that relied on comparing the difference of the means of the pre-test/post-test. We also used an observational methodology for the working sessions from the experimental group. Although the statistical results do not show significant differences between the two groups, the results of the observations exhibited an improvement in visual-motor cognitive skills

    Association analysis of genes involved in maize (Zea mays L.) root development with seedling and agronomic traits under contrasting nitrogen levels

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    Genotypes with large and well distributed root system might have the potential to adapt to soils with limited nutrient availability. For this purpose, an association study (AS) panel consisting of 74 diverse set of inbred maize lines were screened for seedling root traits and adult plant root traits under two contrasting nitrogen (N) levels (low and high N). Allele re-sequencing of RTCL, RTH3, RUM1, and RUL1 genes related to root development was carried out for AS panel lines. Association analysis was carried out between individual polymorphisms, and both seedling and adult plant traits, while controlling for spurious associations due to population structure and kinship relations. Based on the SNPs identified in RTCL, RTH3, RUM1, and RUL1, lines within the AS panel were grouped into 16, 9, 22, and 7 haplotypes, respectively. Association analysis revealed several polymorphisms within root genes putatively associated with the variability in seedling root and adult plant traits development under contrasting N levels. The highest number of significantly associated SNPs with seedling root traits were found in RTCL (19 SNPs) followed by RUM1 (4 SNPs) and in case of RTH3 and RUL1, two and three SNPs, respectively, were significantly associated with root traits. RTCL and RTH3 were also found to be associated with grain yield. Thus considerable allelic diversity is present within the candidate genes studied and can be utilized to develop functional markers that allow identification of maize lines with improved root architecture and yield under N stress conditions

    Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions

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    Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained.This study has been supported, in part, by funds of the consolidated Research Group BIO-157, from the General Secretariat of Universities, Research and Technology of the Ministry of Economy, Innovation, Science and Employment Government of the Junta de Andaluc´ıa (Spain), and by the Research Contract no. C-3650-00 under the program FEDER-INNTERCONECTA from the Spanish Government and European Union FEDER funds. Amalia P´erez-Jim´enez is a recipient of a postdoctoral research fellowship Torres- Quevedo no. PTQ 12-05739

    Evaluación sensorial del vino artesanal de uva Isabella (Vitis labrusca L.)

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    The production and consumption of wine in Colombia have increased over the last decade, especially with the red wines which are preferred by Colombians. Spite the few organoleptic characteristics of the grape Isabella (Vitis Iabrusca L.), it possesses intense flavors and aromas, plus its low sugar content and high concentration of acids, gives it the potential to be used in winemaking. The parameters involved in the sensory evaluation of the wines are the color, aroma and flavor and become sensations perceived in different areas of the tongue. The combination of these causes’s sensations are called wine texture. Sensory analysis of wines through tastings gives rise to a description, that although subjective, generates some impact on the market position ofthis drink. Descriptive tests are the most common and can express the different sensations of wine labeling them by a scale. The aim of this study was to evaluate the organoleptic quality through blind tasting, the artisan wine grape Isabella (Vitis Iabrusca L). We valued three crafted wines, two of them produced in the Pedagogical and Technological University of Colombia, and a third from the town of Cerrito, Valle del Cauca. The panel conformed by 20 tasters evaluated the wines according to the parameters set by the profile sheet given by the International Competition of Wines and Spirits (CINVE). The results showed significant differences between the Paradise wine Portal and the Semi-dry and semi-sweet UPTC wines. The results suggest that the Semi-dry wine obtained the lowest score ranking, as the best sensorial level. The Uptc wines obtained the lowest score, classified as the best in the sensory study.El consumo de vino en Colombia se ha incrementado durante los últimos años, en especial el de los vinos tinto. A pesar de las pocas características vínicas de la uva Isabella (Vitis labrusca L.), su bajo contenido de azúcares y su alta concentración de ácidos le otorgan el potencial para la elaboración de un vino que presenta sabores y aromas intensos. La combinación de color, aroma y sabor provoca la denominada textura del vino. El análisis sensorial de los vinos, a través de catas, da origen a una escala entre los vinos y a una descripción que genera cierto impacto en el posicionamiento de este licor en el mercado. Esta investigación buscó evaluar la calidad organoléptica del vino artesanal de uva Isabella, mediante una cata a ciegas. Se evaluaron tres tipos de vino, dos producidos en la Universidad Pedagógica y Tecnológica de Colombia (semiseco y semidulce) y uno procedente del municipio de Cerrito, Valle del Cauca. El panel conformado por 20 catadores evaluó los vinos de acuerdo con los parámetros establecidos por el Concurso Internacional de Vinos y Espirituosos (CINVE). Los resultados presentaron diferencias significativas entre el vino Portal del Paraíso® y los vinos Uptc. Los vinos Uptc obtuvieron la menor puntuación, catalogándolos, de acuerdo con el CINVE, como los mejores sensorialmente
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