4 research outputs found
A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
A molecular approach was used for selecting polyhydroxyalcanoate
(PHA)-accumulating potential Gram-negative bacteria from different
genera by colony polymerase chain reaction (PCR). Three degenerate
primers were designed for amplifying a fragment from PHA synthase gene
(phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting
PHA-producing bacteria. Thirty-four out of 55 bacterial strains from
the old collection selected using Sudan black B staining were phaC+.
PCR was used for directly selecting 35 new collection bacterial
strains; these strains were phaC+ and their ability to produce PHA was
confirmed by Sudan black B staining. Four specific primers were
designed on genes of Class II PHA biosynthesis operon. These primers
were used for evaluating 9 strains from the old phaC+ collection; 6
showed Class II PHA synthase organisation. 34 from the old and new
bacterial isolation were characterised by 16S ribosomal gene (16S rDNA)
gene partial sequencing. The tool proposed here can be used for better
directing PHA production based on PHA biosynthesis genes and bacterial
genera. Class I or II phaC genes were detected in 9 different genera
and were able to infer the type of polymer produced
A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
A molecular approach was used for selecting polyhydroxyalcanoate
(PHA)-accumulating potential Gram-negative bacteria from different
genera by colony polymerase chain reaction (PCR). Three degenerate
primers were designed for amplifying a fragment from PHA synthase gene
(phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting
PHA-producing bacteria. Thirty-four out of 55 bacterial strains from
the old collection selected using Sudan black B staining were phaC+.
PCR was used for directly selecting 35 new collection bacterial
strains; these strains were phaC+ and their ability to produce PHA was
confirmed by Sudan black B staining. Four specific primers were
designed on genes of Class II PHA biosynthesis operon. These primers
were used for evaluating 9 strains from the old phaC+ collection; 6
showed Class II PHA synthase organisation. 34 from the old and new
bacterial isolation were characterised by 16S ribosomal gene (16S rDNA)
gene partial sequencing. The tool proposed here can be used for better
directing PHA production based on PHA biosynthesis genes and bacterial
genera. Class I or II phaC genes were detected in 9 different genera
and were able to infer the type of polymer produced
Characterization of hydrocarbonoclastic marine bacteria using the 16s rrna gene: a microcosm case study
Algunos microorganismos marinos pueden degradar contaminantes derivados del petr贸leo us谩ndolos como 煤nica fuente de carbono y energ铆a. Este grupo heterog茅neo es llamado bacterias hidrocarburocl谩sticas. Sin embargo, se subestima a aquellas bacterias hidrocarburocl谩sticas que aun no han sido caracterizadas. En este contexto, se llevo a cabo un estudio de microcosmos simulando un ambiente marino impactado con petr贸leo, en Biorreactores de Flujo Ascendente (BFA). A partir de estos microcosmos fueron caracterizados once aislamientos bacterianos. Se determin贸 la ubicaci贸n taxon贸mica de las bacterias identificadas en 4 g茅neros principales por secuenciaci贸n parcial del gen ARNr 16S: Bacillus, Pseudomonas, Halomonas y Haererehalobacter. La investigaci贸n demostr贸 la presencia de bacterias hidrocarburocl谩sticas reconocidas en consorcios y proporcion贸 informaci贸n adicional acerca de Haererehalobater como un nuevo g茅nero de bacterias marinas hidrocarburocl谩sticas. Desde esta perspectiva, se pretendi贸 aportar al conocimiento de la diversidad de las bacterias marinas hidrocarburocl谩sticas y su uso potencial en biorremediaci贸n y otros procesos biotecnol贸gicos
Characterization of hydrocarbonoclastic marine bacteria using the 16s rRNA gene: A microcosm case study
Some marine microorganisms can degrade oil pollutants by using them as their sole carbon and energy sources. Members of this heterogenic group are called hydrocarbonoclastic bacteria. However, an unestimated number of hydrocarbonoclastic marine bacteria have not yet been characterized. In this context, a microcosm study was carried out, simulating a marine environment contaminated with oil in Upstream Flow Bioreactors (UFB). Eleven bacterial isolates were characterized from these microcosms. The taxonomic position of the bacteria identified was determined by partial sequencing of the gene 16S rRNA in 4 major genera: Bacillus, Pseudomonas, Halomonas and Haererehalobacter. This research demonstrated the presence of hydrocarbonoclastic bacteria recognized in consortia and provides additional information about Haererehalobater a new genera of hydrocarbonoclastic marine bacteria. The outlook for better understanding of the diversity of hydrocarbonoclastic marine bacteria and potential uses in bioremediation and other biotechnological processes is discussedAlgunos microorganismos marinos pueden degradar contaminantes derivados del petr贸leo us谩ndolos como 煤nica fuente de carbono y energ铆a. Este grupo heterog茅neo es llamado bacterias hidrocarburocl谩sticas. Sin embargo, se subestima a aquellas bacterias hidrocarburocl谩sticas que aun no han sido caracterizadas. En este contexto, se llevo a cabo un estudio de microcosmos simulando un ambiente marino impactado con petr贸leo, en Biorreactores de Flujo Ascendente (BFA). A partir de estos microcosmos fueron caracterizados once aislamientos bacterianos. Se determin贸 la ubicaci贸n taxon贸mica de las bacterias identificadas en 4 g茅neros principales por secuenciaci贸n parcial del gen ARNr 16S: Bacillus, Pseudomonas, Halomonas y Haererehalobacter. La investigaci贸n demostr贸 la presencia de bacterias hidrocarburocl谩sticas reconocidas en consorcios y proporcion贸 informaci贸n adicional acerca de Haererehalobater como un nuevo g茅nero de bacterias marinas hidrocarburocl谩sticas. Desde esta perspectiva, se pretendi贸 aportar al conocimiento de la diversidad de las bacterias marinas hidrocarburocl谩sticas y su uso potencial en biorremediaci贸n y otros procesos biotecnol贸gico