Analysis of errors in histology by root cause analysis: a pilot study

Introduction. The study objective is to evaluate critical points in the process of pre-analytical histology in an Anatomic Pathology laboratory. Errors are an integral part of human systems, includ- ing the complex system of Anatomic Pathology. Previous studies focused on errors committed in diagnosis and did not consider the issues related to the histology preparation of routine processes. Methods. Root Cause Analysis was applied to the process of histology preparation in order to identify the root cause of each previously identified problem. The analysis started by defining an ?a priori? list of errors that could occur in the histology prepara- tion processes. During a three-month period, a trained technician tracked the errors encountered during the process and reported them on a form. ?Fishbone? diagram and ?Five whys? methods were then applied. Results. 8,346 histological cases were reviewed, for which 19,774 samples were made and from which 29,956 histologies were pre- pared. 132 errors were identified. Errors were detected in each phase: accessioning (6.5%), gross dissecting (28%), processing (1.5%), embedding (4.5%), tissue cutting and slide mounting (23%), coloring, (1.5%), labeling and releasing (35%). Discussion. Root cause analysis is effective and easy to use in clinical risk management. It is an important step for the identifi- cation and prevention of errors, that are frequently due to multi- ple causes. Developing operators? awareness of their central role in the risk management process is possible by targeted training. Furthermore, by highlighting the most relevant points of interest, it is possible to improve both the methodology and the procedural safety

Pressure ulcers management: an economic evaluation

Introduction. Pressure ulcer management represents a growing problem for medical and social health care systems all over the world, particularly in European Union countries where the incidence of pressure ulcers in older persons (> 60 years of age) is predicted to rise. Objectives. The aim of this study was to provide evidence for the lower impact on economic resources of using advanced dressings for the treatment of pressure ulcers with respect to conventional simple dressings. Methods. Two different models of analysis, derived from Activity Based Costing and Health Technology Assessment, were used to measure, over a 30-day period, the direct costs incurred by pressure ulcer treatment for community-residing patients receiving integrated home care. Results. Although the mean cost per home care visit was higher in the advanced dressings patient group than in the simple dressings patient one (? 22.31 versus ? 16.03), analysis of the data revealed that the cost of using advanced dressings was lower due to fewer home care visits (22 versus 11). Conclusion. The results underline the fact that decision-makers need to improve their understanding of the advantages of taking a long-term view with regards to the purchase and use of materials. This could produce considerable savings of resources in addition to improving treatment efficacy for the benefit of patients and the health care system

Calcineurin controls expression of EAAT1/GLAST in mouse and human cultured astrocytes through dynamic regulation of protein synthesis and degradation

Alterations in the expression of glutamate/aspartate transporter (GLAST) have been associated with several neuropathological conditions including Alzheimer\u2019s disease and epilepsy. However, the mechanisms by which GLAST expression is altered are poorly understood. Here we used a combination of pharmacological and genetic approaches coupled with quantitative PCR and Western blot to investigate the mechanism of the regulation of GLAST expression by a Ca2+ /calmodulin-activated phosphatase calcineurin (CaN). We show that treatment of cultured hippocampal mouse and fetal human astrocytes with a CaN inhibitor FK506 resulted in a dynamic modulation of GLAST protein expression, being downregulated after 24\u201348 h, but upregulated after 7 days of continuous FK506 (200 nM) treatment. Protein synthesis, as assessed by puromycin incorporation in neo-synthesized polypeptides, was inhibited already after 1 h of FK506 treatment, while the use of a proteasome inhibitor MG132 (1 \ub5M) shows that GLAST protein degradation was only suppressed after 7 days of FK506 treatment. In astrocytes with constitutive genetic ablation of CaN both protein synthesis and degradation were significantly inhibited. Taken together, our data suggest that, in cultured astrocytes, CaN controls GLAST expression at a posttranscriptional level through regulation of GLAST protein synthesis and degradation

An upper limb Functional Electrical Stimulation controller based on Reinforcement Learning: A feasibility case study.

Controllers for Functional Electrical Stimulation (FES) are still not able to restore natural movements in the paretic arm. In this work, Reinforcement Learning (RL) is used for the first time to control a hybrid upper limb robotic system for stroke rehabilitation in a real environment. The feasibility of the FES controller is tested on one healthy subject during elbow flex-extension in the horizontal plane. Results showed an absolute position error <1.2° for a maximum range of motion of 50°

Psychological impact and health-related quality-of-life outcomes of Mayer-Rokitansky-K&#252;ster-Hauser syndrome : A systematic review and narrative synthesis

Mayer-Rokitansky-K\ufcster-Hauser syndrome causes absence or underdevelopment of uterus and vagina, but women's subjective experience remains understudied. This systematic review was conducted to examine the psychological and health-related quality-of-life outcomes of Mayer-Rokitansky-K\ufcster-Hauser syndrome. In total, 22 articles identified through electronic search matched the inclusion criteria and were included in our review. Mayer-Rokitansky-K\ufcster-Hauser syndrome may be associated with psychological symptoms and impaired quality of life, but especially with poor sexual esteem and genital image. Women may experience difficulties managing intimacy and disclosing to partners. Mothers may be perceived as overinvolved, with consequent negative emotions in women with the disease

The presence of dominant follicles and corpora lutea does not perturb response to controlled ovarian stimulation in random start protocols

The advent of random start protocols to shorten the time needed to store oocytes in women with malignancies has represented an important improvement in the field of fertility preservation. However, Randomized Controlled Trials are difficult to implement in this area and available evidence that supports this approach remains modest. To shed more light on this issue, we compared the follicular development between the ovary carrying the dominant follicle or the corpus luteum and the contralateral resting ovary in 90 women who underwent random start controlled ovarian stimulation (COS). In fact, ovarian response did not differ between the two ovaries. Subgroup analyses according to the phase of the cycle at the initiation of COS, the type of malignancy, the use of letrozole and the magnitude of the ovarian response did not allow to identify any condition showing a difference in the follicular response between the active and the resting ovaries. In conclusion, follicular growth does not seem to be perturbed by the presence of a dominant follicle or a corpus luteum

Assessing opportunities and weaknesses of green hydrogen transport via LOHC through a detailed techno-economic analysis

In the transition towards a more sustainable energy system, hydrogen is seen as the key low-emission energy source. However, the limited H2 volumetric density hinders its transportation. To overcome this issue, liquid organic hydrogen carriers (LOHCs), molecules that can be hydrogenated and, upon arrival, dehydrogenated for H2 release, have been proposed as hydrogen transport media. Considering toluene and dibenzyltoluene as representative carriers, this work offers a systematic methodology for the analysis and the comparison of LOHCs, in view of identifying cost-drivers of the overall value-chain. A detailed Aspen Plus process simulation is provided for hydrogenation and dehydrogenation sections. Simulation results are used as input data for the economic assessment. The process economics reveals that dehydrogenation is the most impactful cost-item, together with the carrier initial loading, the latter related to the LOHC transport distance. The choice of the most suitable molecule as H2 carrier, ultimately, is a trade-off between its hydrogenation enthalpy and cost.(c) 2023 The Author(s). Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC. This is an open access article under the CC BY license (http://creativecommons.org/ licenses/by/4.0/)

Estimating a mean-path from a set of 2-d curves

To perform many common industrial robotic tasks, e.g. deburring a work-piece, in small and medium size companies where a model of the work-piece may not be available, building a geometrical model of how to perform the task from a data set of human demonstrations is highly demanded. In many cases, however, the human demonstrations may be sub-optimal and noisy solutions to the problem of performing a task. For example, an expert may not completely remove the burrs that result in deburring residuals on the work-piece. Hence, we present an iterative algorithm to estimate a noise-free geometrical model of a work-piece from a given dataset of profiles with deburring residuals. In a case study, we compare the profiles obtained with the proposed method, nonlinear principal component analysis and Gaussian mixture model/Gaussian mixture regression. The comparison illustrates the effectiveness of the proposed method, in terms of accuracy, to compute a noise-free profile model of a task