Galactic Nonlinear Dynamic Model

We develop a model for spiral galaxies based on a nonlinear realization of the Newtonian dynamics starting from the momentum and mass conservations in the phase space. The radial solution exhibits a rotation curve in qualitative accordance with the observational data.Comment: 6 pages, 1 figure. Talk given in the 7th Alexander Friedmann International Seminar, June 29 to July 5, 2008, Joao Pessoa, PB, Brazi

Assignment of novel functions to Helicobacter pylori 26695’s genome

Helicobacter pylori is a pathogenic bacterium that colonizes the human epithelia, causing duodenal and gastric ulcers as well as gastric cancer. The genome of H. pylori 26695 has been sequenced and annotated. In addition, two genome-scale metabolic models have been developed. In order to maintain accurate and relevant information on coding sequences (CDS) and to retrieve new information, the assignment of new functions to Helicobacter pylori 26695’s genes was performed. The use of software tools, on-line databases and an annotation pipeline for inspecting each gene allowed the attribution of validated E.C. numbers to metabolic genes, and the assignment of 177 new functions to the CDS of this bacterium. This information provides relevant biological information for the scientific community dealing with this organism and can be used as the basis for a new metabolic model reconstruction.(undefined

Assignment of novel functions to Helicobacter pylori 26695’s genome and reconstruction of a genome-scale metabolic model

Helicobacter pylori is a pathogenic organism associated with human gastric diseases. The development of mathematical models of metabolism is now considered a fundamental part of the study of the cell. For the particular case of microorganisms associated with human diseases, information on metabolic and regulatory networks can be used to understand the molecular factors of the microorganism that are likely to interact with the host and cause diseases. The availability of the genome sequence of H. pylori 26695 and its annotation has allowed in the past the construction of a metabolic model for this organism. The first genome-scale metabolic model for H. pylori 26695 was published in 2002 (iCS291) and a corrected reconstruction was published in 2005 (iIT341 GSM/GPR). The main goal of the present work was to update H. pylori’s genome-scale metabolic model based on the new information made available. For that purposes, using new annotation methodologies and data available in databases, an assignment of novel functions to H. pylori 26695’s genome was performed. For a total of 510 “hypothetical proteins” (almost 1/3 of the genes) identified in the last re-annotation, 137 new functions were attributed. A total of 581 E.C. numbers were assigned to CDS, being 528 complete E.C. numbers. This new information was used as the basis of the model reconstruction. In addition, transport reactions in the model were updated. The biomass equation was reviewed and H. pylori biomass coefficients and composition were adjusted. The obtained model successfully predicted the nutritional requirements and amino acids essentialities, which were experimentally validated. As a result, the present work presents a new H. pylori 26695 genome-scale metabolic model with more accurate and reliable predictions and can be used to identify potential targets for designing more effective drugs for H. pylori inactivation

An algebra of behavioural types

Special thanks to Gérard Boudol, Ilaria Castellani, Silvano Dal Zilio, and Massimo Merro, for fruitful discussions and careful reading of parts of this document. Several anonymous referees made useful comments.We propose a process algebra, the Algebra of Behavioural Types, as a language for typing concurrent objects. A type is a higher-order labelled transition system that characterises all possible life cycles of a concurrent object. States represent interfaces of objects; state transitions model the dynamic change of object interfaces. Moreover, a type provides an internal view of the objects that inhabits it: a synchronous one, since transitions correspond to message reception. To capture this internal view of objects we define a notion of bisimulation, strong on labels and weak on silent actions. We study several algebraic laws that characterise this equivalence, and obtain completeness results for image-finite types.publishersversionpublishe

Custo de utilização de pastagens na Região Sudeste do Brasil.

bitstream/item/65403/1/COT-37-Custo-de-utilizacao-de.pd

Mucosal delivery of tuberculosis vaccines: a review of current approaches and challenges.

Introduction: Tuberculosis (TB) remains a major health threat and it is now clear that the current vaccine, BCG, is unable to arrest the global TB epidemic. A new vaccine is needed to either replace or boost BCG so that a better level of protection could be achieved. The route of entry of Mycobacterium tuberculosis, the causative organism, is via inhalation making TB primarily a respiratory disease. There is therefore good reason to hypothesize that a mucosally delivered vaccine against TB could be more effective than one delivered via the systemic route.Areas covered: This review summarizes the progress that has been made in the area of TB mucosal vaccines in the last few years. It highlights some of the strengths and shortcomings of the published evidence and aims to discuss immunological and practical considerations in the development of mucosal vaccines.Expert opinion: There is a growing body of evidence that the mucosal approach to vaccination against TB is feasible and should be pursued. However, further key studies are necessary to both improve our understanding of the protective immune mechanisms operating in the mucosa and the technical aspects of aerosolized delivery, before such a vaccine could become a feasible, deployable strategy

Genetic improvement of forest tree species.

Brazilian forestry sector is considered one of the most developed in the world, being the base for important industrial segments which use wood as raw material. Tree breeding has played an important role on improving the competitiveness of Brazilian forestry-based companies, especially for its positive reflexes on increasing adaptation, forestry productivity and wood quality. In spite of the importance of other forest trees for the economy, such as Schizolobium, Araucaria, Populus and Hevea, the main genera under genetic improvement in the country are Eucalyptus, Pinus, Acacia and Tectona. They are used by industries like pulp and paper, siderurgy, tannin, chips for exportation and lumber, constituting an important source of revenues for the Brazilian?s economy, besides their positive social and environmental impacts. This paper presents a generic approach to genetic improvement aspects of these four major genera currently undergoing breeding in Brazil.Especial issue

Leg ulcer as a manifestation of eosinophilic vasculitis in a patient with hepatitis C virus infection, medicated with pegylated interferon/ribavirin.

Cryoglobulinaemic vasculitis is a complication of hepatitis C virus (HCV) infection, responding to treatment with pegylated interferon (peg-IFN)/ribavirin (RIB), but vasculitis may first appear after treatment with peg-IFN/RIB. A 35-year-old man with HCV infection presented to our department with a 2-month history of a 3.3×3 cm ulcer localised on the right shin, with a regular border, on a violaceous base. Histopathological examination revealed a leucocytoclastic vasculitis, rich in eosinophils. The patient had been treated with peg-IFN/RIB 10 months prior and treatment was discontinued after 2 months because of the appearance of arthralgias and neuropathy. Laboratory investigations revealed positive cryoglobulins, elevation of rheumatoid factor and reduction of C4 after treatment with peg-IFN/RIB. Dressings with a hydrocellular foam were placed and after 2 months the ulcer resolved. We presented this case because of the rarity of development of a cryoglobulinaemic vasculitis in a patient with HCV infection, previously treated with peg-IFN/RIB

Seleção recorrente recíproca entre populações sintéticas multi-espécies (SRR-PSME) de eucalipto.

Propõe-se estratégia de melhoramento para o eucalipto, que visa contemplar, em um mesmo indivíduo, características desejáveis presentes em diferentes espécies e, simultaneamente, capitalizar heterose para crescimento, advinda de vários pares de espécies, cujas combinações são sabidamente heteróticas. Baseia-se na obtenção de duas populações sintéticas multi-espécies e posterior seleção recorrente recíproca (SRR) entre essas. É denominada seleção recorrente recíproca entre populações sintéticas multi-espécies (SRR-PSME) e melhora os caracteres resistência à seca e às doenças, volume, densidade, rendimento e teor de lignina. São apresentadas opções para composição das populações sintéticas. A SRR-PSME é favorável também à aplicação da seleção genômica.Nota Científica

Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers.

This study aimed to evaluate the genetic diverity of 20 garlic cultivars by using morphological characters and amplified fragment length polymorphism (AFLP) markers to identify possible duplicate cultivars