Results of Blood Serum from Cancer Treated Hamsters with Infrared Thermal Field and Electromagnetic Fields

A project of the influence of Christos Drossinakis on model biophysical, biochemical and biological systems has been conducted. His influence is with infrared thermal field and electromagnetic fields (e.m. fields). In this study an experiment on hamsters with malignant tumors was performed. The hamsters are prepared by Reneta Toshkova. Spectral analysis methods are Non-equilibrium spectrum (NES) and Differential non-equilibrium spectrum (DNES) for 1% solutions of blood serum from hamsters with cancer and healthy hamsters were investigated. Drossinakis had an effect on hamsters with cancer. Results are obtained with the DNES method according control serum samples of hamsters with cancer and healthy hamsters. The spectrum was obtained in the range    (-0.0937 eV; 13.23 μm; 755 cm-1) - (-0.1387 eV; 8.95; μm; 1117 cm-1). The average energy (∆EH...O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of Drossinakis measured by NES- and DNES-methods. The result ∆E=-0.0071±0.0011 eV is according control serum samples of hamsters with cancer and is ∆E=-0.0035±0.0011 eV for healthy hamsters. These results suggest the restructuring of ∆EH...O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. Local extremum was obtained at (-0.1212) eV, which is an indicator of anti-inflammatory effect. Also, a local extremum was obtained at   (-0.1387) eV, which is an indicator of inhibition of development of tumor cells at the molecular level. The effects of Drossinakis influence– anti-inflammatory, antioxidant, development  inhibition of cancer cells are shown. This article deals with the review of the basic biophysical-biochemical and biological processes underlying the influence of Drossinakis. The authors are studying their physical-chemical properties and biophysical and biological effects on the organism of hamsters. Another research by Toshkova have shown that the cancer hamsters influenced by Drossinakis have increased their life span. Keywords: effect of Drossinakis, serum of hamsters, anti-inflammatory, antioxidant, NES, DNES. DOI: 10.7176/JHMN/58-1

Beneficial Effects of Drossinakis Bio-influence (With Infrared Thermal and Electromagnetic Fields) on the Development of Experimental Graffi Myeloid Tumors in Hamsters. Hematological Studies

The aim of the present experiments was to study the effect of bioinfluence - Infrared thermal field (ITF) and electromagnetic fields (e.m. fields) on the progression of experimental tumor – Graffi myeloid tumor in hamsters Spectral analysis methods called Non-equilibrium spectrum (NES) and Differential non-equilibrium spectrum (DNES) for 1% solutions of blood serum from hamsters with cancer and healthy hamsters were investigated. Drossinakis has a beneficial effect on the Graffi tumor-bearing hamsters. Results are obtained with the DNES method - compared to the control serum samples of hamsters with cancer and healthy hamsters. The spectrum was obtained in the range (-0.0937 eV; 13.23 μm; 755 cm-1) - (-0.1387 eV; 8.95; μm; 1117 cm-1). The average energy (∆EH...O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of Drossinakis was measured by NES- and DNES-methods. The result ∆E=-0.0071±0.0011 eV is according control serum samples of hamsters with cancer and ∆E=-0.0035±0.0011 eV is for healthy ones. These results suggest the restructuring of ∆EH...O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. Local extremum was obtained at (-0.1212) eV, which is an indicator of anti-inflammatory effect. Another local extremum was obtained at   (-0.1387) eV, which is an indicator of the tumor-development inhibition at the cellular- and the molecular level. The beneficial effects of Drossinakis are characterized as the anti-inflammatory, anti-oxidant, anti-cancer, anti-stroke,etc. This article deals with the review of the basic biophysical-, biochemical- and biological processes - bio-influenced of Drossinakis. In this sense, the experimental research of  Toshkova and co-authors has shown increased life span of the tumor-bearing hamsters, bio-influenced by Drossinakis. There are new analyses from  hematological studies from Zvetkova. Keywords: Christos Drossinakis - bioinfluence, Infrared thermal field (ITF), Electromagnetic fields,  Experimental myeloid tumor of Graffi,  hematological studies - erythrocytes DOI: 10.7176/JMPB/54-02 Publication date: April 30th 201

Effects of Electromagnetic Fields over DNA in Tumor Diseases, Biophysical and Biochemical Model Systems

Studies were conducted with model systems of influence of Drossinakis with electromagnetic (e.m.) fields and infrared thermal field (ITF) (Ignatov, Mosin, Niggli, Drossinakis, 2013). The purpose of research is to analyze effects over DNA. In the report is carried out analysis of effects over water and physiological saline. Results are achieved with blood serum of hamsters and physiological processes in hamsters with tumors. The analyses with water are conducted using the methods Nonequilibrium Energy Spectrum (NES) and Differential Nonequilibrium Energy Spectrum (DNES) (Antonov, 1992; Ignatov, 1998). Experiments are carried out with the influence of tumor cells of a mouse in water. It is observed reduction of DNES spectrum according to the control sample of cells in healthy animals. (Antonov, 1992). Reduction is also observed in DNES spectrum in blood serum of people having oncological diseases, compared to the one of healthy people (Ignatov, 2012). Such a reduction is most prevalent in (-0.1387 eV; 8.95 µm; 1117 cm-1). In research of the effects of e.m. fields in water and blood serum from hamsters the range is (-0.08 – -0.14 eV) (8.9 –15.5µm) (645–1129 cm-1). Research is conducted for the effects over Graffi tumor that was implanted in hamsters (Toshkova, Ignatov, Zvetkova, Ignatov, Gluhchev, Drossinakis, 2019).  Studies are conducted with pH and oxidation redox potential (ORP) effects of e.m. fields over physiological saline (Gluhchev, Ignatov, Drossinakis, 2019). Keywords: Infrared thermal field (ITF), electromagnetic fields (e.m. fields), experimental Graffi solid tumor, energy spectrum, NES and DNES methods. DOI: 10.7176/JMPB/56-03 Publication date:May 31st 201

Bioinfluence with Infrared Thermal and Electromagnetic Fields as a Therapeutic Approach of Hamsters with Experimental Graffi Myeloid Tumor

The aim of the present experiments was to study the effect of bioinfluence - Infrared thermal field (ITF) and electromagnetic fields (e.m. fields) on the progression of experimental tumor in hamsters.Spectral analysis methods called Non-equilibrium spectrum (NES) and Differential non-equilibrium spectrum (DNES) for 1% solutions of blood serum from hamsters with cancer and healthy hamsters were investigated. Drossinakis had an effect on hamsters with cancer. Results are obtained with the DNES method compared to the control serum samples of hamsters with cancer and healthy hamsters. The spectrum was obtained in the range    (-0.0937 eV; 13.23 μm; 755 cm-1) - (-0.1387 eV; 8.95; μm; 1117 cm-1). The average energy (∆EH...O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of Drossinakis was measured by NES- and DNES-methods. The result ∆E=-0.0071±0.0011 eV is according control serum samples of hamsters with cancer and ∆E=-0.0035±0.0011 eV is for healthy ones. These results suggest the restructuring of ∆EH...O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. Local extremum was obtained at (-0.1212) eV, which is an indicator of anti-inflammatory effect. Another local extremum was obtained at   (-0.1387) eV, which is an indicator of inhibition of development of tumor cells at the molecular level. The effects of Drossinakis influence– anti-inflammatory, antioxidant, and cancer development  inhibition are shown. This article deals with the review of the basic biophysical-biochemical and biological processes underlying the influence of  Drossinakis. The authors are studying their physical-chemical properties and biophysical and biological effects on the organism of hamsters. Another research by Toshkova has shown that the life span of cancer hamsters influenced by Drossinakis has been increased. Keywords: Infrared thermal field (ITF), electromagnetic fields (e.m. fields), experimental Graffi solid tumor DOI: 10.7176/JNSR/9-4-0

Raspodjela energije vodikovih veza i informacijsko-teorijska analiza krvnog seruma hrčka s eksperimentalnim Graffi tumorom

The aim of the present study was to estimate the feasibility of blood serum hydrogen bonds energy distribution, and information-theoretic characteristics, as diagnostic markers for tumor disease in Graffi tumor bearing hamsters. Golden Syrian hamsters were randomly divided into two groups: a healthy control and an experimental one, challenged with Graffi tumor cells. The distributions of hydrogen bonds energies (-E) in the blood serum samples were investigated with a nonequilibrium process of droplets evaporation and sequential measurements of their wetting angle (Non-equilibrium Energy Spectrum (NES)). The measurements show significantly lower average hydrogen bond energy of the experimental samples than the controls. Shifts in NES toward depletion of (-E) at energy levels 0.0937, 0.1187, 0.1212, 0.1337, and 0.1387 eV, and an additional population at 0.0987, 0.1012, 0.1137, and 0.1237 eV energy levels were observed in the experimental samples compared to the controls. Such a pattern points to the possibility of utilizing the f(E) shifts at these energy levels as diagnostic markers of Graffi tumor in hamsters. Information-theoretic measures of Shannon entropy, Variation of information and Transformational information entropy were used to investigate possible differences between the experimental and control spectrum. Although they differ significantly in definitions, a comparison with the three information-theoretic measures did not reveal a statistically significant difference.Cilj ovog istraživanja bio je procijeniti mogućnost upotrebe raspodjele energije vodikovih veza (-E) i informacijskoteorijskih karakteristika u krvnom serumu kao dijagnostičkih markera za tumorsku bolest na modelu hrčka s Graffi-jevim tumorom. Zlatni sirijski hrčci nasumično su podijeljeni u dvije skupine: zdravu kontrolnu i pokusnu skupinu s inokuliranim stanicama Graffi-jevog tumora. Raspodjela energija vodikovih veza (-E) u uzorcima krvnog seruma ispitivana je neravnotežnim procesom isparavanja kapljica i sekvencijalnim mjerenjem njihovog kuta vlaženja (Non-equilibrium Energy Spectrum (NES)). Prema dobivenim mjerenjima prosječna energija vodikove veze eksperimentalnih uzoraka bila je znatno niža od one kontrolnih. U eksperimentalnim uzorcima uočeni su pomaci u NES prema smanjenju energije vodikovih veza na razinama energije od 0,0937, 0,1187, 0,1212, 0,1337 i 0,1387 eV, te dodatna populacija na razinama energije od 0,0987, 0,1012, 0,1137 i 0,1237 eV u usporedbi s kontrolama. Za utvrđivanje mogućih razlika između eksperimentalnog i kontrolnog spektra korištene su informacijsko-teorijske mjere Shannonove entropije, Varijacije informacije i Transformacijske informacijske entropije. Iako su različite u svojim definicijama, nije pronađena statistički značajna razlika između tri informacijsko-teorijske mjere za eksperimentalne i kontrolne uzorke

Effects of Electromagnetic Fields over DNA of Hamsters with Implanted Graffi Tumor

Studies were conducted with model systems of bio influence (Drossinakis’ method) with electromagnetic (e.m.) fields and infrared thermal field (ITF) (Ignatov, Mosin, Niggli, Drossinakis, 2013). The purpose of research is to analyze effects over DNA. Analysis of effects over water and physiological saline is carried out in the report. Results are achieved with blood serum of hamsters and physiological processes in hamsters with tumors. The water analyses have been conducted with Nonequilibrium Energy Spectrum (NES) and Differential Nonequilibrium Energy Spectrum (DNES) methods (Antonov, 1992; Ignatov, 1998). Experiments were carried out about the influence on tumor cells of mice in water.  Reduction of DNES spectrum according to the control sample of cells in healthy animals was observed. (Antonov, 1992). Reduction is also observed in DNES spectrum in blood serum of people having oncological diseases, compared to the one of healthy people (Ignatov, 2012). Such a reduction is most prevalent in (-0.1387 eV; 8.95 µm; 1117 cm-1). In research of the effects of e.m. fields in water and blood serum from hamsters the range is (-0.08 – -0.14 eV) (8.9 –15.5µm) (645–1129 cm-1). Research is conducted for the effects over Graffi tumor that was implanted in hamsters (Toshkova, Ignatov, Zvetkova, Ignatov, Gluhchev, Drossinakis, 2019).  Studies are conducted with pH and oxidation redox potential (ORP) effects of e.m. fields over physiological saline (Gluhchev, Ignatov, Drossinakis, 2019). Keywords: Infrared thermal field (ITF), electromagnetic fields (e.m. fields), experimental Graffi solid tumor, energy spectrum, NES and DNES methods. DOI: 10.7176/JHMN/68-05 Publication date: November 30th 201

Theoretical Analysis of Hydrogen Bonds, Energy Distribution and Information in a 1 % Rosa damascena Mill Oil Solution

The method of Non-equilibrium Energy Spectrum (NES) was applied in measurement of hydrogen bonds energy distribution in 1% Rosa damscena L. oil solution in deionized water. Local maxima in this spectrum were identical with these obtained in investigations of other biologically active solutions and related to particular bio effects as follows: (-0.1387 eV; 8.95 µm; 1117 cm-1). This local maximum is typical for antibacterial, anti-tumor and anti-inflammatory effects. The local maxima at (-0.1212 eV; 10.23 µm; 978 cm-1) and (-0.1262 eV; 9.82 µm; 1018 cm-1) are typical for anti-inflammatory effects and this at (-0.1112 eV; 11.15 µm; 897 cm-1) is typical for effects on the nervous system and nerve conductivity. Information theoretical analysis was performed using the values of Shannon entropy and Transformational information entropy, pointing to hydrogen bonds distribution similarities between Rosa damscena L., V. myrtillus L. and Salvia divinorum Epling. The possible chemical causes of these similarities were identified as antioxidant activity and polyphenols concentration

Učinci vode obogaćene vodikom na hrčke s eksperimentalnim mijeloidnim tumorom

This paper presents the results of an in vivo study of the antitumor effect of hydrogen-rich water in hamsters with experimental myeloid tumors. Hydrogen-rich EVOdrop water (HEW) with a molecular hydrogen (H2) concentration of 1.2 ppm. pH=7.3, and oxidation-reduction potential (ORP) of (-390 mV) as basic parameters were obtained with the EVObooster apparatus. The myeloid tumor was implanted subcutaneously on the back of Golden Syrian hamsters used for the experiment. The effect of HEW on biometric parameters of tumor growth, namely 18 hematological parameters and some hematological biomarkers (indices) was evaluated. The blood sera from experimental and control animals were analyzed using the Non-equilibrium energy spectrum (NES) and the Differential non-equilibrium energy spectrum (DNES) spectral methods. The results showed a protective antitumor effect of HEW seen by a decrease in tumor uptake rate, a decrease in mortality, an increase in survival time, a normalizing effect on hematological parameters and biomarkers (N/LR, WBC/LR, PLT/LR), and an increase in energy between water molecules in blood serum in hamsters with experimental myeloid tumor. The results show that HEW has anticancer potential that could be used with conventional chemotherapeutics to develop new treatment strategies as well as cancer prevention.U radu su prikazani rezultati in vivo istraživanja antitumorskog učinka vode obogaćene vodikom na modelu hrčka s mijeloidnim tumorom. U eksperimentu je korištena voda obogaćena molekularnim vodikom (H2) u koncentraciji od 1.2 ppm, pH=7.3, s oksidacijsko-redukcijskim potencijalom od -390 mV dobivena elektrolizom deionizirane vode pomoću uređaja EVObooster. Suspenzija stanica mijeloidnog tumora injicirana je subkutano u područje leđa hrčaka koji su nasumično svrstani u dvije skupine: 1) skupina u kojoj su hrčci pili vodu obogaćenu vodikom (HEW); 2) skupina u kojoj su hrčci pili deioniziranu vodu (DW); dok su 3) skupina zdrave životinje (kontrola). 25. dan nakon inokulacije mijeloidnog tumora žrtvovane su po tri životinje za procjenu učinka HEW na biometrijske parametre rasta tumora i 18 hematoloških parametara. Ostale životinje su ostavljene za praćenje veličine i brzine rasta tumora te ukupnog preživljenja. Serumi životinja analizirani su i spektralnim metodama neravnotežnog energetskog spektra (NES) i diferencijalnog neravnotežnog energetskog spektra (DNES). Rezultati su pokazali antitumorski učinak vode obogaćene vodikom kroz smanjenje stope rasta tumora, produljenje preživljenja te normalizaciju hematoloških parametara. 30. dan eksperimenta prosječna veličina tumora u skupini HEW je 16.7±4.7 mm, u usporedbi s 20.8±3.3 mm u skupini DW. Ukupna smrtnost životinja postignuta je 40. dan eksperimenta u skupini DW te 57. dan u skupini HEW. Dobiveni su statistički značajni rezultati učinka vode obogaćene vodikom na normalizaciju vrijednosti limfocita i trombocita u krvi te hematoloških indeksa (PLT/LR). Spektralnim metodama utvrđeno je statistički značajno povećanje energije između molekula vode u serumu hrčaka s mijeloičnim tumorom koji su pili HEW. Možemo zaključiti da voda obogaćena vodikom ima antikancerogeni potencijal koji bi se mogao koristiti s konvencionalnim kemoterapeuticima za razvoj novih strategija liječenja, kao i za prevenciju raka

The effects of meloxicam, lornoxicam, ketoprofen, and dexketoprofen on human cervical, colorectal, and mammary carcinoma cell lines

Targeting the inflammation-related molecules with nonsteroidal anti-inflammatory drugs (NSAIDs) represents a promising approach for cancer prevention/therapy. We evaluated the in vitro anticancer effects of meloxicam, lornoxicam, ketoprofen, and dexketoprofen on the proliferation, migration, and apoptosis of human cervical, colorectal, and mammary carcinoma cells. The antiproliferative activity and cytotoxicity of tested NSAIDs on HeLa, HT-29, and MCF-7 cell lines were assessed by the MTT test. The apoptosis-inducing potential was analyzed by fluorescent staining with acridine orange/ethidium bromide and DAPI. Migration activity was assessed by a wound-healing scratch assay. The tested NSAIDs reduced the viability of the used tumor cell lines. The cytomorphological analysis revealed reduced cell density and mitotic activity and the presence of cells with morphological features of early and late apoptosis. Significant inhibition of the migration capacity was established as well. In conclusion, NSAIDs could be candidates for the development of new pharmacological strategies for the treatment and prevention of cancer