Measuring IEEE 802.11p Performance for Active Safety Applications in Cooperative Vehicular Systems

Abstract-In this paper, we present a measurement study of application layer performance in IEEE 802.11p vehicular networks. More specifically, our focus is on active safety applications, which are based on the exchange of beacon messages containing status information between close-by vehicles. We consider two performance metrics relevant to active safety applications: the first is application-layer goodput, which can be used to optimize congestion control techniques aimed at limiting the beaconing load on the wireless channel; the second is the beacon reception rate, which is useful to estimate the level of situation awareness achievable onboard vehicles. Our measurements were conducted using a prototypal, 802.11p compliant communication device developed by NEC, in both stationary and mobile V2V scenarios, and disclosed several useful insights on 802.11p application-level performance. To the best of our knowledge, the ones presented in this paper are the first application-level measurements of IEEE 802.11p based vehicular networks reported in the literature

The IPERMOB System for Effective Real-Time Road Travel Time Measurement and Prediction

Accurate, real-time measurement and estimation of road travel time is considered a central problem in the design of advanced Intelligent Transportation Systems. In particular, whether eective, real-time collection of travel time measurements in a urban area is possible is, to the best of our knowledge, still an open problem. In this paper, we introduce the IPERMOB system for efficient, real-time collection of travel time measurements in urban areas through vehicular networks. We demonstrate that travel time measurements can be accurately estimated onboard GPS-equipped vehicles, and delivered to a centralized server within a few seconds by sending a single message. Furthermore, in IPERMOB locations of travel time checkpoints can be dynamically changed through software reconfiguration, thus at a very limited cost as compared to the enormous costs of, say, installing and/or changing location of automatic vehicle identification equipment. We demonstrate the effectiveness of our approach through extensive travel time collection campaigns. In particular, our technique is shown to estimate travel time with an accuracy below 1%, with two-, three-orders of magnitude savings in both communication and storage resources with respect to existing techniques based on centralized collection of GPS traces. In the last part of the paper, we further show how real-time travel time measurements can be exploited to perform accurate, short range travel time predictions in situations where existing travel time prediction approaches are challenged (e.g., in presence of traffic congestion). The effects of vehicular network penetration rate on accuracy of travel time prediction are also discusse

A Measurement-based Study of Beaconing Performance in IEEE 802.11p Vehicular Networks

Active safety applications for vehicular networks aims at improving safety conditions on the road by raising the level of "situation awareness" onboard vehicles. Situation awareness is achieved through exchange of beacons reporting positional and kinematic data. Two important performance parameters influence the level of situation awareness available to the active safety application: the beacon (packet) delivery rate (PDR), and the packet inter-reception (PIR) time. While measurementbased evaluations of the former metric recently appeared in the literature, the latter metric has not been studied so far. In this paper, for the first time, we estimate the PIR time and its correlation with PDR and other environmental parameters through an extensive measurement campaign based on IEEE 802.11p technology. Our study discloses several interesting insights on PIR times that can be expected in a real-world scenarios, which should be carefully considered by the active safety application designers. A major insight is that the packet inter reception time distribution is a power-law and that long situation awareness black-outs are likely to occur in batch, implying that situation awareness can be severely impaired even when the average beacon delivery rate is relatively high. Furthermore, our analysis shows that PIR and PDR are only loosely (negatively) correlated, and that the PIR time is almost independent of speed and distance between vehicles. A third major contribution of this paper is promoting the Gilbert-Elliot model, previously proposed to model bit error bursts in packet switched networks, as a very accurate model of beacon reception behavior observed in real-world data

Understanding Vehicle-to-Vehicle IEEE 802.11p Beaconing Performance in Real-World Highway Scenarios

Periodic exchange of situational information (beacons) is at the basis of most active safety applications in vehicular environments. Despite its fundamental role in raising the level of "situational awareness" onboard vehicles, very little is known to date on beaconing performance in a real vehicular environment. This paper analyzes the results of two measurement campaigns that have been designed with the purpose of disclosing beaconing performance in a variety of vehicular links, for what concerns vehicle configuration (tall/short), line-of-sight conditions (LOS/NLOS), as well as single-hop or two-hop propagation of the information reported in the beacons. For the first time, beaconing performance is characterized in terms of not only the packet (beacon) delivery rate (PDR), but also in terms of the packet (beacon) inter-reception (PIR) time. The latter metric has been suggested in the literature as more accurately measuring the level of "situation awareness" onboard vehicles than the traditional PDR metric. This paper also presents a simulation-based analysis aimed at estimating the benefit of multi-hop propagation of situational information beyond the second hop of communication. The analysis of the data collected in the measurement campaigns as well as the simulation-based analysis disclose a number of interesting insights which might prove useful in the design of active safety applications. Finally, another major contribution of this paper is promoting the Gilbert-Elliot model, previously proposed to model bit-error bursts in packet switched networks, as a very accurate model of beacon reception behavior observed in real-world scenarios

484. Preclinical Proof of Concept of Transcriptional Silencing and Replacement Strategy for Treatment of Retinitis Pigmentosa Due To RHODOPSIN Mutations

Silencing and replacement strategy is a promising approach to overcome mutational heterogeneity of genetic defects. In autosomal dominant retinitis pigmentosa (adRP) due to rhodopsin gene (RHO) approximately 200 different mutations have been described, posing a challenge for the design of effective therapeutics.We designed a silencing and replacement strategy based on transcriptional silencing through an artificial zinc finger DNA-binding protein lacking effector domains (ZF6DBD), and tested both efficacy and safety in two animal models.In a murine model of adRP, we show that AAV-mediate retinal delivery (AAV2/8-CMV-ZF6-DBD) is associated with selective transcriptional silencing of the human mutated allele resulting in morphological and functional (Electroretinography, ERG a-wave and b-wave responses) rescue. We then tested the effect of transcriptional silencing in the porcine large pre-clinical model. Delivery of a low dose (AAV2/8-CMV-ZF6-DBD, 1×10e10 vector genomes, vg) of the ZF6 transcriptional silencer to the porcine retina resulted in robust transcriptional silencing of the endogenous porcine RHO transcript. Cell sorting of transduced photoreceptors showed an almost complete RHO transcriptional silencing effect (90% RHO transcriptional repression), underscoring the potency of the system. To determine the safety of the zinc-finger silencer we performed extensive RNA-seq analysis on treated and control retinae. The data sets generated demonstrate selective RHO gene transcriptional repression and a remarkably low number of differential expressed genes (DEGs), supporting specificity and thus, safety. The co-administration to the porcine retina of the AAV-ZF6 silencer (AAV2/8-CMV-ZF6-DBD) and the AAV-RHO replacement (5×10e11 vg, AAV2/8-GNAT1-HumanRHO) constructs resulted in a balanced silencing and replacement effect. This data support the use of zinc-finger based RHO transcriptional silencing for the development of a clinical trial for adRP patients

320 transcriptional silencing via synthetic dna binding protein lacking canonical repressor domains as a potent tool to generate therapeutics

Transcription factors (TFs) function by the combined activity of their DNA-binding domains (DBDs) and effector domains (EDs). Here we show that in vivo delivery of an engineered DNA-binding protein uncoupled from the repressor domain entails complete and gene-specific transcriptional silencing. To silence RHODOPSIN (RHO) gain-of-function mutations, we engineered a synthetic DNA-binding protein lacking canonical repressor domains and targeted to the regulatory region of the RHO gene. AAV-mediate retinal delivery at a low dose (AAV2/8-CMV-ZF6-DBD, 1×10e10 vector genomes, vg) in the porcine retina resulted in selective transcriptional silencing of RHO expression. The rod photoreceptors (the RHO expressing cells) transduced cells when isolated by FACS-sorting showed the remarkable 90% RHO transcriptional repression. To evaluate genome-wide transcriptional specificity, we analyzed the porcine retina transcriptome by RNA sequencing (RNA-Seq). The differentially expressed genes (DEGs) analysis showed that only 19 genes were perturbed. In this study, we describe a system based on a synthetic DNA binding protein enabling targeted transcriptional silencing of the RHO gene by in vivo gene transfer. The high rate of transcriptional silencing occurring in transduced cells supports applications of this regulatory genomic interference with a synthetic trans-acting factor for diseases requiring gene silencing in a large number of affected cells, including for instance a number of neurodegeneration disorders. The result support a novel mode of gene targeted silencing with a DNA-binding protein lacking intrinsic activity

Saving temporary exhibitions in virtual environments: The Digital Renaissance of Ulisse Aldrovandi – Acquisition and digitisation of cultural heritage objects

As per the objectives of Project CHANGES, particularly its thematic sub-project on the use of virtual technologies for museums and art collections, our goal was to obtain a digital twin of the temporary exhibition on Ulisse Aldrovandi called “The Other Renaissance”, and make it accessible to users online. After a preliminary study of the exhibition, focusing on acquisition constraints and related solutions, we proceeded with the digital twin creation by acquiring, processing, modelling, optimising, exporting, and metadating the exhibition. We made hybrid use of two acquisition techniques to create new digital cultural heritage objects and environments, and we used open technologies, formats, and protocols to make available the final digital product. Here, we describe the process of collecting and curating bibliographical exhibition (meta) data and the beginning of the digital twin creation to foster its findability, accessibility, interoperability, and reusability. The creation of the digital twin is currently ongoing

Acute cardiac injury after subarachnoid haemorrhage: two case reports

It is well known that cardiopulmonary complications are often associated to subarachnoid haemorrhage. For appropriate therapeutic managing it is very important to distinguish acute coronary syndrome from neurogenic myocardial injury, which is a reversible condition. Furthermore, because the hearts of brain dead patients may be utilized for therapeutic purpose, it has became of importance to rule out erroneous diagnosis of cardiac ischemia in order to avoid rejection of hearts potential suitable for transplantation

The Price of Privacy Control in Mobility Sharing

© 2020 The Society of Urban Technology. One of the main features in mobility sharing applications is the exposure of personal data provided to the system. Transportation and location data can reveal personal habits, preferences, and behaviors, and riders could be keen not to share the exact location of their origin and/or destination. But what is the price of privacy in terms of decreased efficiency of a mobility sharing system? In this paper we address the privacy issues under this point of view, and show how location privacy-preserving techniques could affect the performance of mobility-sharing applications, in terms of both system efficiency and quality of service. To this extent, we first apply different data-masking techniques to anonymize geographical information, and then compare the performance of shareability network-based trip-matching algorithms for ride-sharing, applied to real data and to privacy-preserving data. The goal of the paper is to evaluate the performance of mobility-sharing, privacy-preserving systems, and to shed light on the trade-off between data privacy and its costs. The results show that the total traveled distance increase due to the introduction of data privacy could be bounded if users are willing to spend (or “pay”) for more time in order to share a trip, meaning that data location privacy affects both efficiency and quality of service