Mechanistic analysis of endothelial lipase G promotion of the occurrence and development of cervical carcinoma by activating the phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B/mechanistic target of rapamycin kinase signalling pathway

Lipase G, endothelial type (LIPG) is expressed abundantly in tissues with a high metabolic rate and vascularisation. Research on LIPG has focussed on metabolic syndromes. However, the role of LIPG in providing lipid precursors suggests that it might function in the metabolism of carcinoma cells. Analysis in The Cancer Genome Atlas indicated that patients with cervical carcinoma with high LIPG expression had a lower survival prognosis compared with patients with low LIPG expression. The mechanism underlying the effects of LIPG in cervical carcinoma is unclear. The present study aimed to determine the role of LIPG in cervical carcinoma and its mechanism. The results showed that the LIPG expression level was higher in cervical cancer. Downregulation of LIPG expression inhibited cell migration, invasion, proliferation, and the formation of cell colonies, but increased the rate of apoptosis. The Human papillomavirus E6 protein might reduce the expression of miR-148a-3p, relieve the inhibitory effect of miR-148a-3p on LIPG expression, and promote the progression of cervical cancer through the phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B/mechanistic target of rapamycin kinase signalling pathway.IMPACT STATEMENT What is already known on this subject? LIPG provides lipid precursors, suggesting that it might function in the metabolism of carcinoma cells What do the results of this study add? LIPG might be regulated by HPV16 E6/miR-148a-3p and promote cervical carcinoma progression via the PI3K/AKT/mTOR signalling pathway. What are the implications of these finding for clinical practice and/or further research? The results indicated that novel treatment and diagnosis strategies for cervical carcinoma could be developed related to LIPG. However, the detailed relationship between LIPG and cervical carcinoma remains to be fully determined

Structures and formation mechanism of borides with varied morphologies in cast γ-TiAl alloys

Borides with varied morphologies and structures obtained by changing B content in as-cast Ti-45Al-2Mn-2Nb alloys are investigated. With the increase of B content, morphologies of borides change from curved to straight, and flake shape, ribbon shape, needle/rod shape, and blocky appear in turn. Curved boride particles have a lamellar structure composed of TiB2 and B2 layers, accompanied by the random appearance and distribution of Ti3B4 and Bf-TiB layers. Except for the absence of the B2 layer, straight needle-like and blocky boride particles have roughly the same structure as curved boride particles, revealing that the formation of curved boride particles is accompanied by divorced eutectic with the matrix and internal irregular eutectic, while no eutectics occurs in straight boride particles. The lack of B atoms during solidification in alloys with lower B content results in a less regular structure and higher stacking fault density of curved boride particles compared to straight boride particles in alloys with higher B content. In addition, some needle-like boride particles are observed to have pure B27-TiB structure without intergrowth. The difference in morphologies and structures can be attributed to the difference in formation mechanism and crystallographic structures of the borides

Polymerizable Surfactant Ligand for Stabilization and Film Formation of CsPbBr₃ Nanocrystals

Surfactant ligands are important in the synthesis of inorganic perovskite nanocrystals (NCs), not only for stabilizing NCs but also for surface defect passivation. A new polymerizable surfactant ligand with a multidentate l-cysteine head, a long oleoyl tail, and a polymerizable styrenyl group (NOSVC) is designed for the post-synthesis treatment and stabilization of colloidal CsPbBr3 NCs in this work. 1H nuclear magnetic resonance and X-ray photoelectron spectroscopy analysis show that the l-cysteine head has strong interactions with the NCs. The absolute photoluminescence quantum yields of the colloidal NCs are increased from 45.1% of the pristine NCs stabilized with oleic acid/oleyl amine to 91.8% after NOSVC treatment. NOSVC-stabilized CsPbBr3 colloidal NCs show enhanced stabilities when exposed in polar solvents. The NOSVC-stabilized CsPbBr3 NCs in a solid film state allow for a photopolymerization to be carried out with the assistance of a photoinitiator. The polymerized films of NOSVC-treated NCs exhibit significantly enhanced stability against thermal radiation, ultraviolet irradiation, and humidity. We also fabricated self-healing polymer films incorporating NOSVC-treated CsPbBr3 NCs as a green filter for a white light-emitting diode device. The green light-emitting films are very stable in humid environments, revealing the great application potential of NOSVC-treated CsPbBr3 NCs in flexible display and lighting devices

Molecular understanding of calcium permeation through the open Orai channel.

The Orai channel is characterized by voltage independence, low conductance, and high Ca2+ selectivity and plays an important role in Ca2+ influx through the plasma membrane (PM). How the channel is activated and promotes Ca2+ permeation is not well understood. Here, we report the crystal structure and cryo-electron microscopy (cryo-EM) reconstruction of a Drosophila melanogaster Orai (dOrai) mutant (P288L) channel that is constitutively active according to electrophysiology. The open state of the Orai channel showed a hexameric assembly in which 6 transmembrane 1 (TM1) helices in the center form the ion-conducting pore, and 6 TM4 helices in the periphery form extended long helices. Orai channel activation requires conformational transduction from TM4 to TM1 and eventually causes the basic section of TM1 to twist outward. The wider pore on the cytosolic side aggregates anions to increase the potential gradient across the membrane and thus facilitate Ca2+ permeation. The open-state structure of the Orai channel offers insights into channel assembly, channel activation, and Ca2+ permeation

Voltage Control of Skyrmion Bubbles for Topological Flexible Spintronic Devices

The electric field is an energy-efficient tool that can be leveraged to control spin-orbit coupling. Although Dzyaloshinskii-Moriya interactions (DMI) in magnetic skyrmion systems can be regulated in flat state using an electric field, the control of their flexible behavior has remained elusive so far. Here, the double modulation of strain and voltage effects in a flexible ultrathin heavy metal (HM)/ferromagnetic (FM)/insulator (I) system, demonstrating that the interfacial DMI can be dual controlled via a mechanical stress and a circuit gating voltage at room temperature, is reported. An intensive tuning efficiency (26.7 mJ m(-2) V-1) is obtained while maintaining an excellent mechanical strength, which is a result of Rashba-DMI tuning at the FM/I interface. The result is promising in achieving novel flexible topological devices where low operation voltage and excellent flexibility are required

Influence of Nonmetallic Interstitials on the Phase Transformation between FCC and HCP Titanium: A Density Functional Theory Study

In addition to the common stable and metastable phases in titanium alloys, the face-centered cubic phase was recently observed under various conditions; however, its formation remains largely unclarified. In this work, the effect of nonmetallic interstitial atoms O, N, C and B on the formation of the face-centered cubic phase of titanium was investigated with the density functional theory. The results indicate that the occupancy of O, N, C and B on the octahedral interstitial sites reduces the energy gap between the hexagonal-close-packed (HCP) and face-centered cubic (FCC) phases, thus assisting the formation of FCC-Ti under elevated temperature or plastic deformation. Such a gap further decreases with the increase in the interstitial content, which is consistent with the experimental observation of FCC-Ti under high interstitial content. The relative stability of the interstitial-containing HCP-Ti and FCC-Ti was studied against the physical and chemical origins, e.g., the lattice distortion and the electronic bonding. Interstitial O, N, C and B also reduce the stacking fault energy, thus further benefiting the formation of FCC-Ti

Influence of Nonmetallic Interstitials on the Phase Transformation between FCC and HCP Titanium: A Density Functional Theory Study

In addition to the common stable and metastable phases in titanium alloys, the face-centered cubic phase was recently observed under various conditions; however, its formation remains largely unclarified. In this work, the effect of nonmetallic interstitial atoms O, N, C and B on the formation of the face-centered cubic phase of titanium was investigated with the density functional theory. The results indicate that the occupancy of O, N, C and B on the octahedral interstitial sites reduces the energy gap between the hexagonal-close-packed (HCP) and face-centered cubic (FCC) phases, thus assisting the formation of FCC-Ti under elevated temperature or plastic deformation. Such a gap further decreases with the increase in the interstitial content, which is consistent with the experimental observation of FCC-Ti under high interstitial content. The relative stability of the interstitial-containing HCP-Ti and FCC-Ti was studied against the physical and chemical origins, e.g., the lattice distortion and the electronic bonding. Interstitial O, N, C and B also reduce the stacking fault energy, thus further benefiting the formation of FCC-Ti