Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cycles.

The prospective study including 166 participants aims to evaluate the association between seminal prosaposin and the outcomes of in vitro fertilization (IVF) cycles in humans. The generalized linear model (GLM) was used to analyze the associations between seminal prosaposin concentrations and normal fertilization rates and good embryos proportion. The generalized estimating equation (GEE) was used to evaluate the association between embryo parameters and the prosaposin concentrations. Each model was adjusted for age of the couples, female basal FSH, AFC and BMI, starting dose and oocyte yield of IVF cycles and smoker. GLM models suggested that prosaposin was significantly associated with fertilization rate ( P = 0.005) and good embryo proportion ( P = 0.038) while none of the semen parameters (sperm concentration, motility, progressive motility, normal morphology rate, postwash sperm concentration and motility) was significantly associated with the parameters in the cohort. Using GEE, it was also shown that prosaposin was positively associated with the occurrence of early cleavage and negatively associated with uneven cleavage pattern on day 3. In both the overall population and the normozoospermia patients, the prosaposin was significantly associated with pregnancy with adjustment with covariates. In conclusion, our data suggested that seminal prosaposin concentration could provide more information regarding normal fertilization and embryo development in IVF than traditional semen parameters

Ambient outdoor air pollutants and sex ratio of singletons born after in vitro fertilization: the effect of single blastocyst transfer.

OBJECTIVE(#br)To evaluate the impact of air pollution on the sex ratio in singletons after IVF treatment and to evaluate the influence of the number of and the developmental stage of transferred embryos on the sex ratio.(#br)DESIGN(#br)Retrospective cohort study.(#br)SETTING(#br)University-affiliated IVF unit.(#br)PATIENT(S)(#br)A total of 7,004 singletons born after fresh transfer or frozen-thawed embryo transfer (FET) between January 2013 and December 2017.(#br)INTERVENTION(S)(#br)None.(#br)MAIN OUTCOME MEASURE(S)(#br)Male-to-female ratio in live-born singletons.(#br)RESULT(S)(#br)The estimated medians (interquartile range) of particle matter (PM)10, PM2.5, CO, NO2, O3, and SO2 at the IVF site were 51.4 (39.5-64.6), 27.7 (20.7-37.4), 0.62 (0.5-0.72), 32.5 (25.4-40.1), 79.6 (63.3-96.6), and 11.9 (9.3-15.9) μg/m3, respectively. Multivariate analysis indicated that SO2 was the only pollutant clearly associated with sex ratio. In singletons from single blastocyst transfer (SBT), as indicated by the generalized additive model, the SO2 concentration and sex ratio showed an inverted-U-shape association. In singletons after non-SBT, a monotonic decreasing in the sex ratio was observed with increased SO2 concentration. Compared with the referent category (SO2 < 7.57 μg/m3), the sex ratio at the 5th decile of SO2 (10.81-11.94 μg/m3) was increased by 2.1-fold (95% confidence interval [CI], 1.3-3.14) after adjusting covariates. In singletons born from non-SBT, the sex ratio significantly decreased only in the 9th (odds ratio = 0.69; 95% CI, 0.53-0.90) and 10th (OR = 0.74, 95% CI, 0.56-0.98) deciles.(#br)CONCLUSION(S)(#br)Low concentrations of SO2 showed an association with increased sex ratio in singletons of SBT, while in singletons born from another ET system the sex ratios did not show an association at low concentrations of SO2

Evidence for Positive Selection on a Number of MicroRNA Regulatory Interactions during Recent Human Evolution

MicroRNA (miRNA)–mediated gene regulation is of critical functional importance in animals and is thought to be largely constrained during evolution. However, little is known regarding evolutionary changes of the miRNA network and their role in human evolution. Here we show that a number of miRNA binding sites display high levels of population differentiation in humans and thus are likely targets of local adaptation. In a subset we demonstrate that allelic differences modulate miRNA regulation in mammalian cells, including an interaction between miR-155 and TYRP1, an important melanosomal enzyme associated with human pigmentary differences. We identify alternate alleles of TYRP1 that induce or disrupt miR-155 regulation and demonstrate that these alleles are selected with different modes among human populations, causing a strong negative correlation between the frequency of miR-155 regulation of TYRP1 in human populations and their latitude of residence. We propose that local adaptation of microRNA regulation acts as a rheostat to optimize TYRP1 expression in response to differential UV radiation. Our findings illustrate the evolutionary plasticity of the microRNA regulatory network in recent human evolution

Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cycles

The prospective study including 166 participants aims to evaluate the association between seminal prosaposin and the outcomes of in vitro fertilization (IVF) cycles in humans. The generalized linear model (GLM) was used to analyze the associations between seminal prosaposin concentrations and normal fertilization rates and good embryos proportion. The generalized estimating equation (GEE) was used to evaluate the association between embryo parameters and the prosaposin concentrations. Each model was adjusted for age of the couples, female basal FSH, AFC and BMI, starting dose and oocyte yield of IVF cycles and smoker. GLM models suggested that prosaposin was significantly associated with fertilization rate (P = 0.005) and good embryo proportion (P = 0.038) while none of the semen parameters (sperm concentration, motility, progressive motility, normal morphology rate, postwash sperm concentration and motility) was significantly associated with the parameters in the cohort. Using GEE, it was also shown that prosaposin was positively associated with the occurrence of early cleavage and negatively associated with uneven cleavage pattern on day 3. In both the overall population and the normozoospermia patients, the prosaposin was significantly associated with pregnancy with adjustment with covariates. In conclusion, our data suggested that seminal prosaposin concentration could provide more information regarding normal fertilization and embryo development in IVF than traditional semen parameters

Study the effects of mechanical activation on Li-N-H systems with H-1 and Li-6 solid-state NMR

To gain insight into the effects of mechanical activation (MA) on the hydrogen desorption of the lithium amide (LiNH2) and lithium hydride (LiH) mixture. LiNH2 and LiH + LiNH2 were mechanically activated by high-energy ball milling. The formed products were studied with in situ H-1 and Li-6 nuclear magic angle spinning (MAS) magnetic resonance (NMR) spectroscopy from ambient temperature to 180 degrees C. Up-field chemical shift was observed in Li-6 MAS NMR spectra with increased milling time, indicating that average local electronic structure around Li nuclei was modified during MA. H-1 MAS NMR was used to dynamically probe ammonia release from the activated LiNH2 at temperature as low as 50 degrees C. In the case of activated LiH + LiNH2 mixtures, the H-1 MAS NMR results implied that MA enhanced the dehydrogenation reaction of LiNH2 + LiH = Li2NH + H-2. (c) 2007 Elsevier B.V All rights reservedclose201

Does prolonged pituitary down-regulation with gonadotropin-releasing hormone agonist improve the live-birth rate in in vitro fertilization treatment?

Xiamen City [3502z20111006]Objective: To evaluate the effects of a prolonged duration of gonadotropin-releasing hormone agonist (GnRH-a) in pituitary down-regulation for controlled ovarian hyperstimulation (COH) on the live-birth rate in nonendometriotic women undergoing in vitro fertilization and embryo transfer (IVF-ET). Design: Retrospective cohort study. Setting: University-affiliated hospital. Patient(s): Normogonadotropic women undergoing IVF. Intervention(s): Three hundred seventy-eight patients receiving a prolonged pituitary down-regulation with GnRH-a before ovarian stimulation and 422 patients receiving a GnRH-a long protocol. Main Outcome Measure(s): Live-birth rate per fresh ET. Result(s): In comparison with the long protocol, the prolonged down-regulation protocol required a higher total dose of gonadotropins. A lower serum luteinizing hormone (LH) level on the starting day of gonadotropin and the day of human chorionic gonadotropin (hCG) and a fewer number of oocytes and embryos were observed in the prolonged down-regulation protocol. However, the duration of stimulation and number of high-quality embryos were comparable between the two groups. A statistically significantly higher implantation rate (50.27% vs. 39.69%), clinical pregnancy rate (64.02% vs. 56.87%) and live-birth rate per fresh transfer cycle (55.56% vs. 45.73%) were observed in the prolonged protocol. Conclusion(s): Prolonged down-regulation in a GnRH-a protocol might increase the live-birth rates in normogonadotropic women. (C) 2014 by American Society for Reproductive Medicine

Fusion of gauge-based, reanalysis, and satellite precipitation products using Bayesian model averaging approach : Determination of the influence of different input sources

Selection of the number and which of multisource precipitation datasets is crucially important for precipitation fusion. Considering the effects of different inputs, this study proposes a new framework based on the Bayesian model averaging (BMA) algorithm to integrate precipitation information from gauge-based analysis CPC, reanalysis-derived dataset ERA5, and satellite-retrieval products IMERG-E and GSMaP-RT. The BMA weights were optimized for the period 2001–2010 using daily measurements and then applied to the period 2011–2015 for model validation. Seven BMA-merged precipitation products (i.e., MCE, MCI, MCG, MCEI, MCEG, MCIG, and MCEIG) were thoroughly evaluated across mainland China and then compared against the state-of-the-art ensemble-based product, MSWEP. The results indicate that the BMA predictions performed substantially better than the reanalysis and satellite precipitation datasets in both daily statistics and seasonal analyses. MCE, MCI, and MCEG demonstrated better performances relative to CPC in terms of individual metrics. Moreover, MCI, MCG, and MCEI generally outperformed MSWEP over the entire study area, particularly in local regions, such as southwestern China and the eastern Tibetan Plateau. During Typhoon Rammasun in 2014, MCG and MCEG provided greater detail for heavy rainfall events than the four ensemble members and the MSWEP product. Thus, the performance of the BMA predictions exhibited evident differences because of various input sources. CPC was the major internal influencing factor with the highest weight score. Meanwhile, the increased-input CPC dataset into the BMA-based schemes exerted a significant influence on the precipitation estimates, which markedly facilitated the performance improvement of the fusion model, and its improved degree (greater than 14 %) was obtained using a ‘changed-initial’ comparison method. Our results demonstrate that the developed modifiable BMA framework is useful for analyzing the impacts of ensemble members on BMA predictions and suggests that it is considerate in the use of different input sources for generating ensemble-based precipitation products

Table_3_Day-3-embryo fragmentation is associated with singleton birth weight following fresh single blastocyst transfer: A retrospective study.docx

BackgroundPrevious studies have arguably associated poor embryo morphology with low birth weight in singletons following single embryo transfer. However, the association between birth weight and specific morphological features in the cleavage stage remains less known. The purpose of the study was to investigate whether morphological features of embryos at the cleavage stage affect birth weight following blastocyst transfer.MethodsThe single-center retrospective cohort study included 4,226 singletons derived from fresh single cleavage-stage embryo transfer (ET; n = 1,185), fresh single blastocyst transfer (BT; n = 787), or frozen-thawed single blastocyst transfer (FBT; n = 2,254) between 2016 and 2019. Morphological parameters including early cleavage, day-3 fragmentation, symmetry, blastomere number, and blastocyst morphology were associated with neonatal birth weight and birth weight z-score in multivariate regression models. The models were adjusted for maternal age, body mass index (BMI), parity, peak estradiol level, endometrial thickness, insemination protocol, female etiologies, order of transfer, mode of delivery, and year of treatment.ResultsAdjusted for confounders, day-3 fragmentation was the only morphological feature associated with birth weight and birth weight z-score, while early cleavage, symmetry, blastomere number, and blastocyst morphology were not. Day-3 fragmentation increased the birth weight in both the ET (115.4 g, 95% CI: 26.6–204.2) and BT groups (168.8 g, 95% CI: 48.8–288.8) but not in the FBT group (7.47 g, 95% CI: -46.4 to 61.3). The associations between birth weight and these morphological parameters were confirmed through birth weight z-score analyses. The adjusted odds of large for gestational age (LGA) and high birth weight were also significantly greater in singletons following the transfer of fragmented embryos in the BT group [odds ratio (OR) 3, 95% CI: 1.2–7.51 and OR 3.65, 95% CI: 1.33–10, respectively]. The presence of fragmentation at the cleavage stage also affected the association between the blastocyst morphology and birth weight. Inner cell mass grades were negatively associated with birth weight in blastocysts with day-3 fragmentation but not in blastocysts without.ConclusionsThe birth weight following blastocyst transfer was found to be positively associated with fragmentation at the cleavage stage. The data did not support the argument that transferring a poor-looking embryo may increase the risks of low birth weight. However, concerns for LGA infants remain.</p

Table_5_Day-3-embryo fragmentation is associated with singleton birth weight following fresh single blastocyst transfer: A retrospective study.docx

BackgroundPrevious studies have arguably associated poor embryo morphology with low birth weight in singletons following single embryo transfer. However, the association between birth weight and specific morphological features in the cleavage stage remains less known. The purpose of the study was to investigate whether morphological features of embryos at the cleavage stage affect birth weight following blastocyst transfer.MethodsThe single-center retrospective cohort study included 4,226 singletons derived from fresh single cleavage-stage embryo transfer (ET; n = 1,185), fresh single blastocyst transfer (BT; n = 787), or frozen-thawed single blastocyst transfer (FBT; n = 2,254) between 2016 and 2019. Morphological parameters including early cleavage, day-3 fragmentation, symmetry, blastomere number, and blastocyst morphology were associated with neonatal birth weight and birth weight z-score in multivariate regression models. The models were adjusted for maternal age, body mass index (BMI), parity, peak estradiol level, endometrial thickness, insemination protocol, female etiologies, order of transfer, mode of delivery, and year of treatment.ResultsAdjusted for confounders, day-3 fragmentation was the only morphological feature associated with birth weight and birth weight z-score, while early cleavage, symmetry, blastomere number, and blastocyst morphology were not. Day-3 fragmentation increased the birth weight in both the ET (115.4 g, 95% CI: 26.6–204.2) and BT groups (168.8 g, 95% CI: 48.8–288.8) but not in the FBT group (7.47 g, 95% CI: -46.4 to 61.3). The associations between birth weight and these morphological parameters were confirmed through birth weight z-score analyses. The adjusted odds of large for gestational age (LGA) and high birth weight were also significantly greater in singletons following the transfer of fragmented embryos in the BT group [odds ratio (OR) 3, 95% CI: 1.2–7.51 and OR 3.65, 95% CI: 1.33–10, respectively]. The presence of fragmentation at the cleavage stage also affected the association between the blastocyst morphology and birth weight. Inner cell mass grades were negatively associated with birth weight in blastocysts with day-3 fragmentation but not in blastocysts without.ConclusionsThe birth weight following blastocyst transfer was found to be positively associated with fragmentation at the cleavage stage. The data did not support the argument that transferring a poor-looking embryo may increase the risks of low birth weight. However, concerns for LGA infants remain.</p

Table_4_Day-3-embryo fragmentation is associated with singleton birth weight following fresh single blastocyst transfer: A retrospective study.docx

BackgroundPrevious studies have arguably associated poor embryo morphology with low birth weight in singletons following single embryo transfer. However, the association between birth weight and specific morphological features in the cleavage stage remains less known. The purpose of the study was to investigate whether morphological features of embryos at the cleavage stage affect birth weight following blastocyst transfer.MethodsThe single-center retrospective cohort study included 4,226 singletons derived from fresh single cleavage-stage embryo transfer (ET; n = 1,185), fresh single blastocyst transfer (BT; n = 787), or frozen-thawed single blastocyst transfer (FBT; n = 2,254) between 2016 and 2019. Morphological parameters including early cleavage, day-3 fragmentation, symmetry, blastomere number, and blastocyst morphology were associated with neonatal birth weight and birth weight z-score in multivariate regression models. The models were adjusted for maternal age, body mass index (BMI), parity, peak estradiol level, endometrial thickness, insemination protocol, female etiologies, order of transfer, mode of delivery, and year of treatment.ResultsAdjusted for confounders, day-3 fragmentation was the only morphological feature associated with birth weight and birth weight z-score, while early cleavage, symmetry, blastomere number, and blastocyst morphology were not. Day-3 fragmentation increased the birth weight in both the ET (115.4 g, 95% CI: 26.6–204.2) and BT groups (168.8 g, 95% CI: 48.8–288.8) but not in the FBT group (7.47 g, 95% CI: -46.4 to 61.3). The associations between birth weight and these morphological parameters were confirmed through birth weight z-score analyses. The adjusted odds of large for gestational age (LGA) and high birth weight were also significantly greater in singletons following the transfer of fragmented embryos in the BT group [odds ratio (OR) 3, 95% CI: 1.2–7.51 and OR 3.65, 95% CI: 1.33–10, respectively]. The presence of fragmentation at the cleavage stage also affected the association between the blastocyst morphology and birth weight. Inner cell mass grades were negatively associated with birth weight in blastocysts with day-3 fragmentation but not in blastocysts without.ConclusionsThe birth weight following blastocyst transfer was found to be positively associated with fragmentation at the cleavage stage. The data did not support the argument that transferring a poor-looking embryo may increase the risks of low birth weight. However, concerns for LGA infants remain.</p