Modulation of the UV-B-induced Oxidative Stress and Apoptosis in HaCaT Cell Line with Calluna vulgaris Extract

The reactive oxygen species (ROS) production due to ultraviolet B (UV-B) exposure is extremely harmful to the skin. It causes lesions of DNA, proteins and lipids and leads to cellular death. In the present study the UV-B-induced ROS and subsequent apoptosis in the human keratinocyte cell line (HaCaT) were counterbalanced by a plant extract with antioxidant capacity. Some molecules modulated by common heather (Calluna vulgaris) (CV) extract through which this may exert its photoprotective effects were also identified. The ROS were evaluated with CM-H2DCFDA assay, while apoptosis and Bax-α/Bcl-xL molecules with ELISA. The extract was standardized according to its polyphenolic content and the most important biologically active compounds, such as hyperozid, quercetin, isoquercetin, kampferol were evidenced by high-performance liquid chromatography. The UV-B induced ROS production occurred at its highest level at 2 h after the exposure of the HaCaT cells, while apoptosis later, at 4 h. The most significant changes in Bax-α and Bcl-XL proteins induced by UV-B, as well as the highest effect of the extract on apoptosis, were both registered at 4 h. The CV extract decreased concentration- and time-dependently the UV-B-induced ROS production and prevented apoptosis. These effects of CV occurred, at least to a certain extent, due to the modulation of Bax-α/Bcl-XL proteins. These findings suggest that skin cells could be protected from some of the UV-B-induced harmful effects by the administration of the CV extract, which may be further exploited as a potential photoprotective agent

Peptidomimetic antibiotics disrupt the lipopolysaccharide transport bridge of drug-resistant Enterobacteriaceae

The rise of antimicrobial resistance poses a substantial threat to our health system, and, hence, development of drugs against novel targets is urgently needed. The natural peptide thanatin kills Gram-negative bacteria by targeting proteins of the lipopolysaccharide transport (Lpt) machinery. Using the thanatin scaffold together with phenotypic medicinal chemistry, structural data, and a target-focused approach, we developed antimicrobial peptides with drug-like properties. They exhibit potent activity against Enterobacteriaceae both in vitro and in vivo while eliciting low frequencies of resistance. We show that the peptides bind LptA of both wild-type and thanatin-resistant Escherichia coli and Klebsiella pneumoniae strains with low-nanomolar affinities. Mode of action studies revealed that the antimicrobial activity involves the specific disruption of the Lpt periplasmic protein bridge

The MAGPI Survey: impact of environment on the total internal mass distribution of galaxies in the last 5 Gyr

We investigate the impact of environment on the internal mass distribution of galaxies using the Middle Ages Galaxy Properties with Integral field spectroscopy (MAGPI) survey. We use 2D resolved stellar kinematics to construct Jeans dynamical models for galaxies at mean redshift z ∼ 0.3, corresponding to a lookback time of 3–4 Gyr. The internal mass distribution for each galaxy is parametrized by the combined mass density slope γ (baryons + dark matter), which is the logarithmic change of density with radius. We use a MAGPI sample of 28 galaxies from low-to-mid density environments and compare to density slopes derived from galaxies in the high density Frontier Fields clusters in the redshift range 0.29 < z < 0.55, corresponding to a lookback time of ∼5 Gyr. We find a median density slope of γ = −2.22 ± 0.05 for the MAGPI sample, which is significantly steeper than the Frontier Fields median slope (γ = −2.00 ± 0.04), implying the cluster galaxies are less centrally concentrated in their mass distribution than MAGPI galaxies. We also compare to the distribution of density slopes from galaxies in ATLAS3D at z ∼ 0, because the sample probes a similar environmental range as MAGPI. The ATLAS3D median total slope is γ = −2.25 ± 0.02, consistent with the MAGPI median. Our results indicate environment plays a role in the internal mass distribution of galaxies, with no evolution of the slope in the last 3–4 Gyr. These results are in agreement with the predictions of cosmological simulations

The SAMI Galaxy Survey: a statistical approach to an optimal classification of stellar kinematics in galaxy surveys

Large galaxy samples from multi-object IFS surveys now allow for a statistical analysis of the z~0 galaxy population using resolved kinematics. However, the improvement in number statistics comes at a cost, with multi-object IFS survey more severely impacted by the effect of seeing and lower S/N. We present an analysis of ~1800 galaxies from the SAMI Galaxy Survey and investigate the spread and overlap in the kinematic distributions of the spin parameter proxy $\lambda_{Re}$ as a function of stellar mass and ellipticity. For SAMI data, the distributions of galaxies identified as regular and non-regular rotators with \textsc{kinemetry} show considerable overlap in the $\lambda_{Re}$-$\varepsilon_e$ diagram. In contrast, visually classified galaxies (obvious and non-obvious rotators) are better separated in $\lambda_{Re}$ space, with less overlap of both distributions. Then, we use a Bayesian mixture model to analyse the observed $\lambda_{Re}$-$\log(M_*/M_{\odot})$ distribution. Below $\log(M_{\star}/M_{\odot})\sim10.5$, a single beta distribution is sufficient to fit the complete $\lambda_{Re}$ distribution, whereas a second beta distribution is required above $\log(M_{\star}/M_{\odot})\sim10.5$ to account for a population of low-$\lambda_{Re}$ galaxies. While the Bayesian mixture model presents the cleanest separation of the two kinematic populations, we find the unique information provided by visual classification of kinematic maps should not be disregarded in future studies. Applied to mock-observations from different cosmological simulations, the mixture model also predicts bimodal $\lambda_{Re}$ distributions, albeit with different positions of the $\lambda_{Re}$ peaks. Our analysis validates the conclusions from previous smaller IFS surveys, but also demonstrates the importance of using kinematic selection criteria that are dictated by the quality of the observed or simulated data.Comment: 30 pages and 17 figures, accepted for publication in MNRAS. Abstract abridged for Arxiv. The key figures of the paper are: 3, 7, 8, and 1

Different higher order kinematics between star-forming and quiescent galaxies based on the SAMI, MAGPI, and LEGA-C surveys

We present the first statistical study of spatially integrated non-Gaussian stellar kinematics spanning 7 Gyr in cosmic time. We use deep, rest-frame optical spectroscopy of massive galaxies (stellar mass ⁠) at redshifts z = 0.05, 0.3, and 0.8 from the SAMI, MAGPI, and LEGA-C surveys, to measure the excess kurtosis h4 of the stellar velocity distribution, the latter parametrized as a Gauss–Hermite series. We find that at all redshifts where we have large enough samples, h4 anticorrelates with the ratio between rotation and dispersion, highlighting the physical connection between these two kinematic observables. In addition, and independently from the anticorrelation with rotation-to-dispersion ratio, we also find a correlation between h4 and M⋆, potentially connected to the assembly history of galaxies. In contrast, after controlling for mass, we find no evidence of independent correlation between h4 and aperture velocity dispersion or galaxy size. These results hold for both star-forming and quiescent galaxies. For quiescent galaxies, h4 also correlates with projected shape, even after controlling for the rotation-to-dispersion ratio. At any given redshift, star-forming galaxies have lower h4 compared to quiescent galaxies, highlighting the link between kinematic structure and star-forming activity

Evolution in the orbital structure of quiescent galaxies from MAGPI, LEGA-C, and SAMI surveys: direct evidence for merger-driven growth over the last 7 Gyr

We present the first study of spatially integrated higher-order stellar kinematics over cosmic time. We use deep rest-frame optical spectroscopy of quiescent galaxies at redshifts z = 0.05, 0.3, and 0.8 from the SAMI, MAGPI, and LEGA-C surveys to measure the excess kurtosis h4 of the stellar velocity distribution, the latter parametrized as a Gauss-Hermite series. Conservatively using a redshift-independent cut in stellar mass (⁠⁠) and matching the stellar-mass distributions of our samples, we find 7σ evidence of h4 increasing with cosmic time, from a median value of 0.019 ± 0.002 at z = 0.8 to 0.059 ± 0.004 at z = 0.06. Alternatively, we use a physically motivated sample selection based on the mass distribution of the progenitors of local quiescent galaxies as inferred from numerical simulations; in this case, we find 10σ evidence. This evolution suggests that, over the last 7 Gyr, there has been a gradual decrease in the rotation-to-dispersion ratio and an increase in the radial anisotropy of the stellar velocity distribution, qualitatively consistent with accretion of gas-poor satellites. These findings demonstrate that massive galaxies continue to accrete mass and increase their dispersion support after becoming quiescent

Canagliflozin and renal outcomes in type 2 diabetes and nephropathy

BACKGROUND Type 2 diabetes mellitus is the leading cause of kidney failure worldwide, but few effective long-term treatments are available. In cardiovascular trials of inhibitors of sodium–glucose cotransporter 2 (SGLT2), exploratory results have suggested that such drugs may improve renal outcomes in patients with type 2 diabetes. METHODS In this double-blind, randomized trial, we assigned patients with type 2 diabetes and albuminuric chronic kidney disease to receive canagliflozin, an oral SGLT2 inhibitor, at a dose of 100 mg daily or placebo. All the patients had an estimated glomerular filtration rate (GFR) of 30 to &lt;90 ml per minute per 1.73 m2 of body-surface area and albuminuria (ratio of albumin [mg] to creatinine [g], &gt;300 to 5000) and were treated with renin–angiotensin system blockade. The primary outcome was a composite of end-stage kidney disease (dialysis, transplantation, or a sustained estimated GFR of &lt;15 ml per minute per 1.73 m2), a doubling of the serum creatinine level, or death from renal or cardiovascular causes. Prespecified secondary outcomes were tested hierarchically. RESULTS The trial was stopped early after a planned interim analysis on the recommendation of the data and safety monitoring committee. At that time, 4401 patients had undergone randomization, with a median follow-up of 2.62 years. The relative risk of the primary outcome was 30% lower in the canagliflozin group than in the placebo group, with event rates of 43.2 and 61.2 per 1000 patient-years, respectively (hazard ratio, 0.70; 95% confidence interval [CI], 0.59 to 0.82; P=0.00001). The relative risk of the renal-specific composite of end-stage kidney disease, a doubling of the creatinine level, or death from renal causes was lower by 34% (hazard ratio, 0.66; 95% CI, 0.53 to 0.81; P&lt;0.001), and the relative risk of end-stage kidney disease was lower by 32% (hazard ratio, 0.68; 95% CI, 0.54 to 0.86; P=0.002). The canagliflozin group also had a lower risk of cardiovascular death, myocardial infarction, or stroke (hazard ratio, 0.80; 95% CI, 0.67 to 0.95; P=0.01) and hospitalization for heart failure (hazard ratio, 0.61; 95% CI, 0.47 to 0.80; P&lt;0.001). There were no significant differences in rates of amputation or fracture. CONCLUSIONS In patients with type 2 diabetes and kidney disease, the risk of kidney failure and cardiovascular events was lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years

WAYS FOR DEVELOPMENT OF RURAL COMMUNITIES IN VALCEA COUNTY

Compilation of the ways for developing a county level, helps to: create a vision about community that we want in the future, provide a complete picture of how the economy, environment, infrastructure can improve the county to set development goals and priorities that are reflected in measures to achieve the objectives. Also, the development of these paths of development may underlie a guide for improving community life and to reduce disparities. In this work we performed analysis of investment programs that benefit local communities in Valcea County in the period 2008-2016. Finally, the County development strategy based on RDP measures was set up for the period 2014-2020

ANALYSIS OF THE SERVICES ROLE IN THE RURAL DEVELOPMENT OF ROMANIA

Services include a highly heterogeneous range of economic activities which are characterized by different manufacturing processes, different delivery modalities, service providers and consumers with different behaviors, various market structures. The main characteristic features of the service are: immateriality and intangibility; unable to be stored; simultaneity of production and consumption; inability to be sustainable; inseparability of the services provider and the person of the user; heterogeneity; lack of properties. The study of various classification systems is particularly interesting for understanding the nature and diversity for services, analysis of different systems applicable to services and their evolution over time, and the correlations between services and other economic activities they serve. The market has very different characteristics depending on the type of service, level of competition, and the state can intervene in the market for the normal conduct of market mechanisms. Regulation can take three forms: technical regulations, administrative and price controls. It was also treated the issue service in rural areas by providing classification and analysis in some ways