601 research outputs found
Comparison of Four Commercially Available Group B Streptococcus Molecular Assays Using Remnant Rectal-Vaginal Enrichment Broths
The incidence of neonatal Group B streptococcal (GBS) disease has significantly declined since the widespread implementation of prenatal screening of expectant mothers for urogenital and gastrointestinal tract GBS colonization. Screening methods have evolved from exclusively culture-based approaches to more rapid and highly sensitive molecular methods. We chose to evaluate the performance of four commercially available GBS molecular tests for detection of GBS colonization using 299 antepartum rectal-vaginal specimens submitted to our laboratory for routine GBS screening. In 97% of instances, there was agreement between all three systems. When testing 1, 6, and 12 samples simultaneously, all methods performed comparably, but the ARIES® GBS assay required the least total hands-on time and the illumigene® Group B Streptococcus assay required the most hands-on time
Evaluation of T2Candida Panel for detection of Candida in peritoneal dialysates
Fungal peritonitis in the peritoneal dialysis population is difficult to diagnose promptly due to the inherently slow cultivation-based methods currently required for identification of peritonitis pathogens. Because of the moderate risk for severe complications, the need for rapid diagnostics is considerable. One possible solution to this unmet need is the T2Candida Panel, a new technology designed to detect the most common pathogenic Candida spp. directly from whole blood specimens in as little as a few hours. We hypothesized that this technology could be applied to the detection of Candida in peritoneal dialysate, a matrix not currently approved by the Food and Drug Administration for testing by this system. Remnant dialysate samples from three healthy (noninfected) pediatric peritoneal dialysis patients were spiked with Candida glabrata, serially diluted, and tested in triplicate with unaltered dialysate specimens. The assay detected C. glabrata in 100% of spiked dialysate samples across the full spectrum of dilutions tested, and no assay inhibition or cross-reactivity was noted. These findings suggest one of possibly more applications of this technology. The positive clinical implications of this test will continue to be realized as its use is validated in peritoneal dialysate and other patient specimen types
Performance of two Askaryan Radio Array stations and first results in the search for ultra-high energy neutrinos
Ultra-high energy neutrinos are interesting messenger particles since, if
detected, they can transmit exclusive information about ultra-high energy
processes in the Universe. These particles, with energies above
, interact very rarely. Therefore, detectors that
instrument several gigatons of matter are needed to discover them. The ARA
detector is currently being constructed at South Pole. It is designed to use
the Askaryan effect, the emission of radio waves from neutrino-induced cascades
in the South Pole ice, to detect neutrino interactions at very high energies.
With antennas distributed among 37 widely-separated stations in the ice, such
interactions can be observed in a volume of several hundred cubic kilometers.
Currently 3 deep ARA stations are deployed in the ice of which two have been
taking data since the beginning of the year 2013. In this publication, the ARA
detector "as-built" and calibrations are described. Furthermore, the data
reduction methods used to distinguish the rare radio signals from overwhelming
backgrounds of thermal and anthropogenic origin are presented. Using data from
only two stations over a short exposure time of 10 months, a neutrino flux
limit of is
calculated for a particle energy of 10^{18}eV, which offers promise for the
full ARA detector.Comment: 21 pages, 34 figures, 1 table, includes supplementary materia
Measurement of the multi-TeV neutrino cross section with IceCube using Earth absorption
Neutrinos interact only very weakly, so they are extremely penetrating.
However, the theoretical neutrino-nucleon interaction cross section rises with
energy such that, at energies above 40 TeV, neutrinos are expected to be
absorbed as they pass through the Earth. Experimentally, the cross section has
been measured only at the relatively low energies (below 400 GeV) available at
neutrino beams from accelerators \cite{Agashe:2014kda, Formaggio:2013kya}. Here
we report the first measurement of neutrino absorption in the Earth, using a
sample of 10,784 energetic upward-going neutrino-induced muons observed with
the IceCube Neutrino Observatory. The flux of high-energy neutrinos transiting
long paths through the Earth is attenuated compared to a reference sample that
follows shorter trajectories through the Earth. Using a fit to the
two-dimensional distribution of muon energy and zenith angle, we determine the
cross section for neutrino energies between 6.3 TeV and 980 TeV, more than an
order of magnitude higher in energy than previous measurements. The measured
cross section is (stat.) (syst.)
times the prediction of the Standard Model \cite{CooperSarkar:2011pa},
consistent with the expectation for charged and neutral current interactions.
We do not observe a dramatic increase in the cross section, expected in some
speculative models, including those invoking new compact dimensions
\cite{AlvarezMuniz:2002ga} or the production of leptoquarks
\cite{Romero:2009vu}.Comment: Preprint version of Nature paper 10.1038/nature2445
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