A Tale of Two Sylamores: Understanding Relationships Among Land Use, Nutrients, and Aquatic Communities Across a Subsidy-Stress Gradient

Agricultural land use is known to degrade aquatic systems with high inputs of nutrients, sediments, and pesticides. Increased nutrients can lead to increased algal growth and thus possible hypoxic conditions in slow moving water, while increased sediment loads have been shown to obstruct light and reduce substrate stability. These conditions negatively impact primary producers, macroinvertebrates, and fish. However, small-scale changes in land use can subsidize an aquatic ecosystem instead, where an increase in nutrients allows nutrient-limited biota to flourish, and minor increases in sedimentation may help support populations of collector-filterers. The stimulation in performance caused by small disturbances is part of the subsidy-stress gradient, where increasing perturbation subsidizes an ecosystem until a certain threshold is reached, at which a decline in performance and increased variability starts to occur. The North and South Sylamore watersheds in north Arkansas provide a useful template to investigate the subsidy-stress gradient in relation to land use. North Sylamore flows through the Ozark National Forest and has a heavily forested catchment, while South Sylamore flows through mostly private land, some of which is pasture (23%). Physicochemical, macroinvertebrate, and fish data were collected from multiple sites within each watershed to determine if South Sylamore is exhibiting a response to pasture/agriculture characteristic of a subsidy-stress gradient. Sites within South Sylamore had significantly higher nitrate levels, larger macroinvertebrate populations dominated by collector-filterers, and greater abundance of algivorous fish, suggesting South Sylamore may be subsidized by the surrounding pastoral lands. However, South Sylamore also had a significantly lower proportional abundance of sensitive macroinvertebrate taxa and more unique tolerant fish taxa, suggesting South Sylamore is experiencing stress as well. Habitat quality of South Sylamore could be improved by restoration of trees within the riparian zone. Monitoring aquatic systems for subsidy-stress responses can inform restoration/management decisions and guide intervention prior to watersheds and aquatic communities becoming overly stressed

Differential signalling through ALK-1 and ALK-5 regulates leptin expression in Mesenchymal Stem Cells

Leptin plays a central role in maintaining energy balance, with multiple other systemic effects. Despite leptin importance in peripheral regulation of mesenchymal stem cells (MSC) differentiation, little is known on its expression mechanism. Leptin is often described as adipokine, while it is expressed by other cell types. We have recently shown an in vitro leptin expression, enhanced by glucocorticoids in synovial fibroblasts. Here, we investigated leptin expression in MSC from bone marrow (BM-MSC), cord matrix (UMSC), and primary and dedifferentiated chondrocytes (DCH). Results showed that BM-MSC, but not UMSC, expressed leptin that was strongly enhanced by glucocorticoids. Interestingly, chondrocytes gained leptin expression progressively with dedifferentiation. This dedifferentiation was correlated with downregulation of ALK-5 expression, Smad2 phosphorylation (p-Smad2), and gain of ALK-1 expression and Smad1/5 phosphorylation (p-Smad1/5). TGF-β1 was shown to signal via ALK-5-Smad2/3 and/or ALK-1-Smad1/5 pathways. In BM-MSC, TGF-β1 increased p-Smad2 expression and markedly inhibited endogenous- and glucocorticoidinduced leptin expression, while ALK-5 inhibitor (SB431542) induced and restored this expression. In addition, both prednisolone and SB431542 increased p-Smad1/5 expression. These results suggested ALK-5-Smad2 pathway as inhibitor of leptin expression, while ALK-1-Smad1/5 as activator. Indeed, Smad1 expression silencing induced leptin expression inhibition. Furthermore, prednisolone enhanced the expression of TGF-βRII while decreasing p-Smad2 in BM-MSC and SVF but not in UMSC. In vitro differentiation revealed differential osteogenic potential in SVF, BM-MSC and UMSC that correlates to their leptin expression potential. Our results suggest that ALK-1/ALK-5 balance regulates leptin expression in MSC. It also underlines UMSC as leptin non-producer MSC for cell therapy protocols where leptin expression is not suitable

Measuring the Pulse Duration of a Femtosecond Laser Using Intensity Autocorrelation

In this experiment, an intensity autocorrelator is set up in order to measure the pulse duration of a passively mode-locked Titanium-Sapphire laser with a power of 267mW producing femtosecond pulses. Then this measurement, as well as a measurement of the laser’s spectrum, is used to test the Uncertainty Principle. Intensity autocorrelation is a well-established technique for measuring pulse duration, and is among the more intuitive techniques for this purpose, which is why it was selected for this experiment. The experimental setup was computerized. The delay of one half of the pulse was controlled by a motorized translation stage which was itself controlled by a computer which could scan across the zero delay point of the pulse, and the data from the detector went to a digital oscilloscope and was then saved by a computer. Dispersion was compensated for by negative dispersion mirrors in the laser itself. To calculate the duration of the pulse, it was assumed that the pulses took the shape of sech^2(x). The duration of the laser pulses was determined to be 12.9 fs. The standard deviation of the pulse in time was found to be 6.6 fs, and the standard deviation of the pulse in energy was 1.15*10^(-20) J. When multiplied, the standard deviations give a value of 7.6*10^(-35) Js or 0.72 ħ, which is greater than the theoretical minimum limit given by the Uncertainty Principle of 0.50 ħ

Die Handwerkssprache der Drucker und Setzer

Die Seminararbeit beschreibt die Drucker- und Setzersprache einhergehend mit der Entwicklung dieses Berufsstandes. Nach einer kurzen Begriffsklärung wird die Geschichte des Druckereiwesens nachgezeichnet, um danach auf die Merkmale der Druckersprache einzugehen. Einen eigenen Teil dieser Arbeit bildet die Vorstellung besonders humoristischer Begriffe, die die Druckerzunft hervorgebracht hat. Dabei wird versucht, auf die etymologischen Ursprünge solcher Termini einzugehen. Im Anhang findet sich ein Wörterbuch der Drucker- und Setzersprache, welches aus verschiedenen Quellen zusammengetragen wurde. Durch die Kombination von Historismen seit etwa 1440 und neuer Fachtermini bis 2016 bietet dieses Wörterbuch einen Abriss der Drucker- und Setzersprache von ihrem Beginn bis heute.:1. Einleitung 02 2. Begriffliche Abgrenzungen 02 3. Geschichte des Druckens mit beweglichen Lettern 03 4. Charakteristika der Druckersprache 05 5. Humorismen und ihre Etymologie 07 6. Lexikologische Hintergründe 09 7. Lexikographische Hintergründe 10 Literaturverzeichnis 13 Anhang 1: Vorstellungsgespräche im Zeitwandel 14 Anhang 2: Wörterbuch der Druckersprache 1

Analysis of pre-weaning feeding policies and other risk factors influencing growth rates in calves on 11 commercial dairy farms

Growth rates in pre-weaned calves influence their health, age at first calving and lifetime productivity. Many farms restrict milk rations to encourage solid feed intake and facilitate early weaning, but this can compromise growth. This study determined the milk feeding policies and associated growth rates on 11 commercial dairy farms in South East England, each following their normal management regime. Between 26 and 54 heifers were recruited per farm, providing a final cohort of 492, of which 71% were pure Holstein. Information on calf rearing practices (feeding, weaning, housing) and health was collected via questionnaires and weekly observations. Estimates of actual milk fed (kg solids) between 1 and 63 days were calculated for individual calves. Morphometric data (weight, height, length) were taken at weeks 1, 5 and 9 and at a median age of 7.5 months and growth rates were calculated. Most calves were fed milk replacer via automated feeders (four farms), teat feeder (one) or buckets (four) whereas two farms provided drums of acidified waste milk. Farms fed between 4 and 6 l/day of milk at mixing rates of 10% to 15%, providing 400 to 900 g/day of milk solids. Both skeletal growth rates and average daily weight gain (ADG) increased in the second month of life compared with the first: height growth from 0.17±0.14 to 0.25±0.16 cm/day and ADG from 0.48±0.25 to 0.71±0.28 kg/day. Post-weaning heifers up to 7.5 months had height increases of 0.16±0.035 cm/day and ADG of 0.83±0.16 kg/day. From 1 to 63 days 70% of calves had growth rates <0.7 kg/day and of these 19.6% gained <0.5 kg/day. Mean ADG before 9 weeks varied between farms from 0.52±0.30 to 0.75±0.20 kg/day. This was related to the amount of milk fed at both a farm and individual calf level. Increasing the total milk solids fed between 1 and 63 days from 20.4 to 46.3 kg (the 10th to 90th percentile observed) was associated with an increase of 0.11 kg/day ADG. All farms had a wide variation in growth rates despite single feeding policies. Higher circulating immunoglobulin G and IGF1 concentrations were associated with better growth, whereas low temperatures in month of birth, high scores for diarrhoea, respiratory and umbilical disease and large birth size reduced growth. Many commercially grown dairy heifers therefore experienced growth restriction in the pre-weaned period, potentially reducing their health, welfare and productivity

Strategic plan 2020-2023

The overall goal of this plan is to expand and diversify audiences through increased accessibility and enhanced public spaces where the museum will be able to tell more nuanced stories about the whole of South Carolina's military history