972 research outputs found
Problem gambling: a suitable case for social work?
Problem gambling attracts little attention from health and social care agencies
in the UK. Prevalence surveys suggest that 0.6% of the population are
problem gamblers and it is suggested that for each of these individuals,
10–17 other people, including children and other family members, are
affected. Problem gambling is linked to many individual and social problems
including: depression, suicide, significant debt, bankruptcy, family conflict,
domestic violence, neglect and maltreatment of children and offending.
This makes the issue central to social work territory. Yet, the training of
social workers in the UK has consistently neglected issues of addictive
behaviour. Whilst some attention has been paid in recent years to substance
abuse issues, there has remained a silence in relation to gambling
problems. Social workers provide more help for problems relating to addictions
than other helping professions. There is good evidence that treatment,
and early intervention for gambling problems, including psycho-social and
public health approaches, can be very effective. This paper argues that
problem gambling should be moved onto the radar of the social work profession,
via inclusion on qualifying and post-qualifying training programmes
and via research and dissemination of good practice via institutions such as
the Social Care Institute for Excellence (SCIE).
Keywords: problem gambling; addictive behaviour; socia
A whole-cell biosensor for the detection of gold
Geochemical exploration for gold (Au) is becoming increasingly important to the mining industry. Current processes for Au analyses require sampling materials to be taken from often remote localities. Samples are then transported to a laboratory equipped with suitable analytical facilities, such as Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) or Instrumental Neutron Activation Analysis (INAA). Determining the concentration of Au in samples may take several weeks, leading to long delays in exploration campaigns. Hence, a method for the on-site analysis of Au, such as a biosensor, will greatly benefit the exploration industry. The golTSB genes from Salmonella enterica serovar typhimurium are selectively induced by Au(I/III)-complexes. In the present study, the golTSB operon with a reporter gene, lacZ, was introduced into Escherichia coli. The induction of golTSB::lacZ with Au(I/III)-complexes was tested using a colorimetric β-galactosidase and an electrochemical assay. Measurements of the β-galactosidase activity for concentrations of both Au(I)- and Au(III)-complexes ranging from 0.1 to 5 µM (equivalent to 20 to 1000 ng g⁻¹ or parts-per-billion (ppb)) were accurately quantified. When testing the ability of the biosensor to detect Au(I/III)-complexes(aq) in the presence of other metal ions (Ag(I), Cu(II), Fe(III), Ni(II), Co(II), Zn, As(III), Pb(II), Sb(III) or Bi(III)), cross-reactivity was observed, i.e. the amount of Au measured was either under- or over-estimated. To assess if the biosensor would work with natural samples, soils with different physiochemical properties were spiked with Au-complexes. Subsequently, a selective extraction using 1 M thiosulfate was applied to extract the Au. The results showed that Au could be measured in these extracts with the same accuracy as ICP-MS (P<0.05). This demonstrates that by combining selective extraction with the biosensor system the concentration of Au can be accurately measured, down to a quantification limit of 20 ppb (0.1 µM) and a detection limit of 2 ppb (0.01 µM).Carla M. Zammit, Davide Quaranta, Shane Gibson, Anita J. Zaitouna, Christine Ta, Joël Brugger, Rebecca Y. Lai, Gregor Grass, Frank Reit
Edging your bets: advantage play, gambling, crime and victimisation
Consumerism, industrial development and regulatory liberalisation have underpinned the ascendance of gambling to a mainstream consumption practice. In particular, the online gambling environment has been marketed as a site of ‘safe risks’ where citizens can engage in a multitude of different forms of aleatory consumption. This paper offers a virtual ethnography of an online ‘advantage play’ subculture. It demonstrates how advantage players have reinterpreted the online gambling landscape as an environment saturated with crime and victimisation. In this virtual world, advantage play is no longer simply an instrumental act concerned with profit accumulation to finance consumer desires. Rather, it acts as an opportunity for individuals to engage in a unique form of edgework, whereby the threat to one’s well-being is tested through an ability to avoid crime and victimisation. This paper demonstrates how mediated environments may act as sites for edgeworking and how the potential for victimisation can be something that is actively engaged with
Influence of the substrate-induced strain and irradiation disorder on the Peierls transition in TTF-TCNQ microdomains
The influence of the combined effects of substrate-induced strain, finite
size and electron irradiation-induced defects have been studied on individual
micron-sized domains of the organic charge transfer compound
tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) by temperature-dependent
conductivity and current-voltage measurements. The individual domains have been
isolated by focused ion beam etching and electrically contacted by focused ion
and electron beam induced deposition of metallic contacts. The
temperature-dependent conductivity follows a variable range hopping behavior
which shows a crossover of the exponent as the Peierls transition is
approached. The low temperature behavior is analyzed within the segmented rod
model of Fogler, Teber and Shklowskii, as originally developed for a
charge-ordered quasi one-dimensional electron crystal. The results are compared
with data obtained on as-grown and electron irradiated epitaxial TTF-TCNQ thin
films of the two-domain type
Using participatory and creative methods to facilitate emancipatory research with people facing multiple disadvantage: a role for health and care professionals
Participatory and creative research methods are a powerful tool for enabling active engagement in the research process of marginalised people. It can be particularly hard for people living with multiple disadvantage, such as disabled people from ethnic minority backgrounds, to access research projects that are relevant to their lived experience. This article argues that creative and participatory methods facilitate the co-researchers’ engagement in the research process, which thus becomes more empowering. Exploring the congruence of these methods with their professional ethos, health and care professionals can use their skills to develop them further. Both theory and practice examples are presented
Functional and Biogenetical Heterogeneity of the Inner Membrane of Rat-Liver Mitochondria
Rat liver mitochondria were fragmented by a combined technique of swelling, shrinking, and sonication. Fragments of inner membrane were separated by density gradient centrifugation. They differed in several respects: electronmicroscopic appearance, phospholipid and cytochrome contents, electrophoretic behaviour of proteins and enzymatic activities.
Three types of inner membrane fractions were isolated. The first type is characterized by a high activity of metal chelatase, low activities of succinate-cytochrome c reductase and of glycerolphosphate dehydrogenase, as well as by a high phospholipid content and low contents of cytochromes aa3 and b.
The second type displays maximal activities of glycerolphosphate dehydrogenase and metal chelatase, but contains relatively little cytochromes and has low succinate-cytochrome c reductase activity.
The third type exhibits highest succinate-cytochrome c reductase activity, a high metal chelatase activity and highest cytochrome contents. However, this fraction was low in both glycerolphosphate dehydrogenase activity and phospholipid content. This fraction was also richest in the following enzyme activities: cytochrome oxidase, oligomycin-sensitive ATPase, proline oxidase, 3-hydroxybutyrate dehydrogenase and rotenone-sensitive NADH-cytochrome c reductase.
Amino acid incorporation in vitro and in vivo in the presence of cycloheximide occurs predominantly into inner membrane fractions from the second type.
These data suggest that the inner membrane is composed of differently organized parts, and that polypeptides synthesized by mitochondrial ribosomes are integrated into specific parts of the inner membrane
Structure and dynamics of ring polymers: entanglement effects because of solution density and ring topology
The effects of entanglement in solutions and melts of unknotted ring polymers
have been addressed by several theoretical and numerical studies. The system
properties have been typically profiled as a function of ring contour length at
fixed solution density. Here, we use a different approach to investigate
numerically the equilibrium and kinetic properties of solutions of model ring
polymers. Specifically, the ring contour length is maintained fixed, while the
interplay of inter- and intra-chain entanglement is modulated by varying both
solution density (from infinite dilution up to \approx 40 % volume occupancy)
and ring topology (by considering unknotted and trefoil-knotted chains). The
equilibrium metric properties of rings with either topology are found to be
only weakly affected by the increase of solution density. Even at the highest
density, the average ring size, shape anisotropy and length of the knotted
region differ at most by 40% from those of isolated rings. Conversely, kinetics
are strongly affected by the degree of inter-chain entanglement: for both
unknots and trefoils the characteristic times of ring size relaxation,
reorientation and diffusion change by one order of magnitude across the
considered range of concentrations. Yet, significant topology-dependent
differences in kinetics are observed only for very dilute solutions (much below
the ring overlap threshold). For knotted rings, the slowest kinetic process is
found to correspond to the diffusion of the knotted region along the ring
backbone.Comment: 17 pages, 11 figure
Release of a New Forage Bermudagrass Cultivar from the USDA-NPGS Cynodon Collection
Warm-season perennial grasses are the backbone of the pasture-based livestock industry in the southeastern USA. In Florida specifically, bahiagrass (Paspalum notatum Flugge) and bermudagrass (Cynodon spp.) support 1 million head of cattle and 15,000 beef cattle operations. Bermudagrass is the most widely planted forage species in the southeastern USA, planted in approximately 15 million ha and used for grazing, hay and silage. The genus Cynodon is native to southern Africa and germplasm collections have revealed a high degree of genetic variability within the genus. The United States Department of Agriculture National Plant Germplasm System (USDA-NPGS) maintains a collection of bermudagrass plant introduction (PIs) in Griffin, GA, USA and the USDA Georgia Coastal Plains Experiment Station, Tifton, GA, maintains additional forage germplasm. Multi-location trials were established in 2014 in four states (FL, GA, NC and OK) to screen the collection for herbage accumulation (HA) and nutritive value (NV). Due to the large genotype by environment interaction for HA across states, we focused on selecting accessions adapted to South Georgia and Florida. Several PIs showed improved HA and NV compared to ‘Tifton 85’ across several trials and years. Particularly, PI 316510 produced high HA in Citra, FL and Tifton, GA, had improved NV traits, and faster establishment compared to Tifton 85. We confirmed that PI 316510 is tetraploid by chromosome counts and flow cytometry. The PI 316510 has been released by the University of Florida under the name “Newell”
Potentials of Mean Force for Protein Structure Prediction Vindicated, Formalized and Generalized
Understanding protein structure is of crucial importance in science, medicine
and biotechnology. For about two decades, knowledge based potentials based on
pairwise distances -- so-called "potentials of mean force" (PMFs) -- have been
center stage in the prediction and design of protein structure and the
simulation of protein folding. However, the validity, scope and limitations of
these potentials are still vigorously debated and disputed, and the optimal
choice of the reference state -- a necessary component of these potentials --
is an unsolved problem. PMFs are loosely justified by analogy to the reversible
work theorem in statistical physics, or by a statistical argument based on a
likelihood function. Both justifications are insightful but leave many
questions unanswered. Here, we show for the first time that PMFs can be seen as
approximations to quantities that do have a rigorous probabilistic
justification: they naturally arise when probability distributions over
different features of proteins need to be combined. We call these quantities
reference ratio distributions deriving from the application of the reference
ratio method. This new view is not only of theoretical relevance, but leads to
many insights that are of direct practical use: the reference state is uniquely
defined and does not require external physical insights; the approach can be
generalized beyond pairwise distances to arbitrary features of protein
structure; and it becomes clear for which purposes the use of these quantities
is justified. We illustrate these insights with two applications, involving the
radius of gyration and hydrogen bonding. In the latter case, we also show how
the reference ratio method can be iteratively applied to sculpt an energy
funnel. Our results considerably increase the understanding and scope of energy
functions derived from known biomolecular structures
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