Conceptual Modeling for Web Site Development

Today’s web site development practice lacks suitable software engineering principles. In this paper we argue to adopt state-of-the-art software engineering principles and tools in order to improve web site development

The Role of Polysorbate 80 and HPβCD at the Air-Water Interface of IgG Solutions

Purpose: To test the hypothesis of surface displacement as the underlying mechanism for IgG stabilization by polysorbates and HPβCD against surface-induced aggregation. Methods: Adsorption/desorption-kinetics of IgG-polysorbate 80/-HPβCD were monitored. Maximum bubble pressure method was used for processes within seconds from surface formation. Profile analysis tensiometry was applied over long periods and to assess surface rheologic properties. Additionally, the kinetics of adsorption, desorption and surface displacement was followed by a double-capillary setup of the profile analysis tensiometer, allowing drop bulk exchange. Results: Weak surface activity for HPβCD vs. much higher surface activity for polysorbate 80 was shown. Protein-displacement when exceeding a polysorbate 80 concentration close to the CMC and a lack of protein displacement for HPβCD was observed. The drop bulk exchange experiments show IgG displacement by polysorbate 80 independent of the adsorption order. In contrast, HPβCD coexists with IgG at the air-water interface when the surface layer is built from a mixed IgG-HPβCD-solution. Incorporation of HPβCD in a preformed IgG-surface-layer does not occur. Conclusions: The results confirm surface displacement as the stabilization mechanism of polysorbate 80, but refute the frequently held opinion, that HPβCD stabilizes proteins against aggregation at the air-water interface in a manner comparable to non-ionic surfactant

Entwicklung eines Data Warehouse für das Produktionscontrolling:Konzepte und Erfahrungen

Aufgabe eines Data Warehouse ist die schnelle und flexible Bereitstellung entscheidungsrelevanter Daten. Es stellt damit - je nach Interpretation - eine Weiterentwicklung der Entscheidungsunterstützungssysteme oder eine auf Analyseaufgaben ausgerichtete Datenbank dar. Um seine Aufgabe erfüllen zu können, muß ein Data Warehouse heterogene Datenquellen zu einer stabilen, konsistenten Datenbasis zusammenführen, Detaildaten für die analytischen Auswertungen (vor-)verdichten sowie auch zeitraumbezogene Längsschnittanalysen unterstützen. Die Entwicklung eines Warehouse unterscheidet sich deshalb in vielen Punkten von der Entwicklung eines traditionellen, transaktionsorientierten Anwendungssystems. Der folgende Beitrag diskutiert, welche Aufgaben jeweils in den verschiedenen Phasen einer Data Wareouse-Entwicklung anfallen und wie diese Aufgaben durchgeführt werden können. Die vorgestellten Konzepte und Erfahrungen sind Ergebnisse eines Kooperationsprojekts zwischen dem Institut und einem großen Maschinenbauunternehmen.<br/

Genotyping of Environmental and Clinical Stenotrophomonas maltophilia Isolates and their Pathogenic Potential

Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequencing distinguished 10 subgroups. For 8 of them, the same strain composition was shown with both typing methods. A subset of 59 isolates representative for the gyrB groups was further investigated with regards to their pathogenic properties in a virulence model using Dictyostelium discoideum and Acanthamoeba castellanii as host organisms. A clear tendency towards accumulation of virulent strains could be observed for one group with A. castellanii and for two groups with D. discoideum. Several virulent strains did not cluster in any of the genetic groups, while other groups displayed no virulence properties at all. The amoeba pathogenicity model proved suitable in showing differences in S. maltophilia virulence. However, the model is still not sufficient to completely elucidate virulence as critical for a human host, since several strains involved in human infections did not show any virulence against amoeba

Futterqualität von Bioraffinerie-Produkten aus unterschiedlichen Silagen

Ziel dieser Studie war es, die Futterqualität von Presssaft und Presskuchen zu analysieren. Die Proteinkonzentration ist im Presssaft höher (204 und 208 g/kg TM für Rotklee-Gras und Rotklee), dennoch kann die Futter-Einsatzmenge aufgrund der geringeren Aminosäurequalität nach oben begrenzt sein

Einsatz von Kleegrassilage-Presssaftkonzentrat in der Mastgeflügel-Fütterung

Ziel dieser Studie war es, verschiedene Beimischungsraten (0 %, 3 %, 6 %, 9 %) eines aus Kleegrassilage hergestellten Proteinkonzentrats zur Fütterung von Bio-Masthähnchen zu testen. Die Tageszunahmen waren bei der höchsten Einsatzmenge signifikant niedriger

Einfluss der Verfütterung von Kleegrassilage-Presskuchen auf Bio-Milchkühe

Ziel dieser Studie war es, Einmischungsraten von Presskuchen für die Fütterung von Bio-Milchkühen zu vergleichen. Der höchste Anteil (V2: 50%) reduzierte die Futteraufnahme. Es wurden weder Auswirkungen auf die Milchleistung noch auf die Energieversorgung und die Rückenfettdicke festgestellt

Interatomic and Intermolecular Coulombic Decay

Interatomic or intermolecular Coulombic decay (ICD) is a nonlocal electronic decay mechanism occurring in weakly bound matter. In an ICD process, energy released by electronic relaxation of an excited atom or molecule leads to ionization of a neighboring one via Coulombic electron interactions. ICD has been predicted theoretically in the mid nineties of the last century, and its existence has been confirmed experimentally approximately ten years later. Since then, a number of fundamental and applied aspects have been studied in this quickly growing field of research. This review provides an introduction to ICD and draws the connection to related energy transfer and ionization processes. The theoretical approaches for the description of ICD as well as the experimental techniques developed and employed for its investigation are described. The existing body of literature on experimental and theoretical studies of ICD processes in different atomic and molecular systems is reviewed. © 2020 American Chemical Societ

Rats overexpressing the dopamine transporter display behavioral and neurobiological abnormalities with relevance to repetitive disorders

The dopamine transporter (DAT) plays a pivotal role in maintaining optimal dopamine signaling. DAT-overactivity has been linked to various neuropsychiatric disorders yet so far the direct pathological consequences of it has not been fully assessed. We here generated a transgenic rat model that via pronuclear microinjection overexpresses the DAT gene. Our results demonstrate that DAT-overexpression induces multiple neurobiological effects that exceeded the expected alterations in the corticostriatal dopamine system. Furthermore, transgenic rats specifically exhibited behavioral and pharmaco-therapeutic profiles phenotypic of repetitive disorders. Together our findings suggest that the DAT rat model will constitute a valuable tool for further investigations into the pathological influence of DAT overexpression on neural systems relevant to neuropsychiatric disorders