Synthesis of Copper Oxide Nanoparticles in Droplet Flow Reactors

Synthesis of metal oxide nanoparticles within droplet flow reactors is advantageous over batch synthesis due to the elimination of concentration and temperature gradients inside the reactor and prevention of reactor fouling. We present results on the synthesis of copper oxide nanoparticles using aqueous droplets of copper acetate and acetic acid inside a bulk stream of sodium hydroxide in 1-octanol. Varying the copper acetate, acetic acid, and sodium hydroxide concentration resulted in needle-like and plate-like nanoparticles of varying sizes. The rate of mass transfer from the bulk to the droplet phase was found to increase with flow rate and addition of surfactants

Thermal behavior of the STM tip under laser irradiation

This thesis discusses the thermal behavior of the STM tip under laser irradiation. The thermal expansion of the tip was researched with varying laser spot size, frequency, location, and power. In order to determine the thermal expansion of the STM tip, the behavior in both the time and frequency domain were investigated. By employing the FFT analysis, the noise of the thermal behavior in the frequency domain was greatly reduced when compared to the time domain behavior, allowing for higher resolution expansions. With noise reduced, a thermal expansion of 1 nm, equating to a 0.03 K average temperature rise across the tip was found. Besides the heating and expansion of the STM tip, the thermal diffusivity of the PtIr STM tip was calculated using the TET and Characteristic Point methods, and found to be within 3% of the expected value

Rhetoric and Social Struggle: In Search of the Topoi of Violence.

The rhetorical study of violence tends to examine violence within larger generic boundaries such as social movements studies or war rhetoric. In order to work toward a generalizable rhetorical theory of violence and discourse, this study examines texts which justify violence across generic boundaries. Accordingly, four case studies individually examine texts which justify political violence. This study compares and contrasts the rhetorical strategies of George Bush, the Unabomber, Earth First! and Abbie Hoffman. This study concludes that there are no universal strategies among the four case studies in the justification of violence. However, there appears to be a continuum of rhetorical strategies which rhetors follow depending on whether they are seeking to reinforce social institutions through violence, or destroy social institutions through violence

TSPO ligands promote cholesterol efflux and suppress oxidative stress and inflammation in choroidal endothelial cells

Choroidal endothelial cells supply oxygen and nutrients to retinal pigment epithelial (RPE) cells and photoreceptors, recycle metabolites, and dispose of metabolic waste through the choroidal blood circulation. Death of the endothelial cells of the choroid may cause abnormal deposits including unesterified and esterified cholesterol beneath RPE cells and within Bruch’s membrane that contribute to the progression of age-related macular degeneration (AMD), the most prevalent cause of blindness in older people. Translocator protein (TSPO) is a cholesterol-binding protein that is involved in mitochondrial cholesterol transport and other cellular functions. We have investigated the role of TSPO in choroidal endothelial cells. Immunocytochemistry showed that TSPO was localized to the mitochondria of choroidal endothelial cells. Choroidal endothelial cells exposed to TSPO ligands (Etifoxine or XBD-173) had significantly increased cholesterol efflux, higher expression of cholesterol homeostasis genes (LXRα, CYP27A1, CYP46A1, ABCA1 and ABCG1), and reduced biosynthesis of cholesterol and phospholipids from [14C]acetate, when compared to untreated controls. Treatment with TSPO ligands also resulted in reduced production of reactive oxygen species (ROS), increased antioxidant capacity, and reduced release of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and VEGF) induced by oxidized LDL. These data suggest TSPO ligands may offer promise for the treatment of AMD

Effects of Insular Cortex Lesions on Conditioned Taste Aversion and Latent Inhibition in the Rat

The present study tested the hypothesis that lesions of the insular cortex of the rat retard the acquisition of conditioned taste aversions (CTAs) because of an impairment in the detection of the novelty of taste stimuli. Demonstrating the expected latent inhibition effect, nonlesioned control subjects acquired CTAs more rapidly when the conditioned stimulus (0.15% sodium saccharin) was novel rather than familiar (achieved by pre-exposure to the to-be-conditioned taste cue). However, rats with insular cortex lesions acquired taste aversions at the same slow rate regardless of whether the saccharin was novel or familiar. The pattern of behavioural deficits obtained cannot be interpreted as disruptions of taste detection or stimulus intensity, but is consistent with the view that insular cortex lesions disrupt taste neophobia, a dysfunction that consequently retards CTA acquisition because of a latent inhibition-like effect

Insular Cortex Lesions and Morphine-Induced Suppression of Conditioned Stimulus Intake in the Rat

The present experiment examined the influence of insular cortex (IC) lesions on the intake of a taste stimulus in a consummatory procedure that used morphine as the unconditioned stimulus. In normal rats, morphine caused a rapid reduction in saccharin intake when the taste was novel but not when it was familiar. Irrespective of stimulus novelty, morphine had little influence on the saccharin consumption of IC-lesioned rats. The results are discussed in terms of a lesion-induced disruption of (i) a reward comparison mechanism and (ii) the perception of taste novelty

The piezoresistance of aluminum alloy interconnect structures

The effects of applied strain on the resistivity of Al thin film metallization interconnects have been measured with a novel methodology that uses thermal expansion mismatch to produce the strain. The interconnect volumetric strain is induced by thermal cycling of passivated and unpassivated interconnects between ≈70 and 373 K. The coefficient of piezoresistivity, defined as dρ/dϵvdρ/dϵv, where ρ=resistivity and ϵvϵv=volumetric strain, is determined by properly accounting for the degree of interconnect constraint and thermal expansion mismatch strain induced during temperature changes. The volumetric strains are calculated for unpassivated and passivated lines of varying thickness and width. A model which incorporates the geometrical and piezoresistance effects on the measured interconnect resistance during temperature changes is described. The coefficient of piezoresistivity is calculated by a fitting procedure which provides an accurate and consistent fit for both unpassivated and passivated interconnects of different geometries and different strain states. The measured coefficient dρ/dϵvdρ/dϵv is 2.0×10−52.0×10−5 Ω cm in tension, similar to earlier results in bulk Al samples measured in compression but significantly higher than values recently measured in Al interconnects. The application of the calibrated coefficient of piezoresistivity for the measurement of electromigration-induced stresses in novel interconnect test structures will be described. © 1999 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70378/2/JAPIAU-85-3-1943-1.pd

Toward economic evaluation of climate change impacts : a review and evaluation of studies of the impact of climate change

Efforts to access climate change have generally been unsuccessful in describing the economic damages (or benefits) associated with climate change or the functional relationship of damage (or benefits) to climate. Existing integrated economic studies have developed an aggregate damage estimate for the United States associated with equilibrium doubled trace gas climate that is unlikely to occur for 100 years or more. These estimates are used to extrapolate damages to other regions and over time. There is little or no basis for such extrapolation. It is possible to introduce climate explicitly into standard economic models but such models have generally not been estimated. Potentially affected sectors include 1) forestry and ecosystems, 2) agriculture, 3) coast, 4) fishers, 5) water resources, and 6) communities and households. An impact classification system is developed that considers short and long run flexibility to adapt to climate change, the existing knowledge or capacity to adapt, and the degree to which climate matters after adaptation (i.e., the degree to which damages can be avoided).Supported by the MIT Joint Program on the Science and Policy of Global Change, and NOAA

Substrate Binding by the Catalytic Domain and Carbohydrate Binding Module of Ruminococcus flavefaciens FD-1 Xyloglucanase/ Endoglucanase

Binding and thermodynamic properties of a carbohydrate binding module (CBM) and a glycoside hydrolase family 44 xyloglucanase/endoglucanase catalytic domain (CD) fromRuminococcus flavefaciens, both when separate and when linked to each other, have been quantified when binding various β-1,4-linked glucans and xylans. The three constructs bind cellotetraose, cellopentaose, and cellohexaose with association constants that increase with chain length. The CBM does not bind xylotetraose, xylopentaose, or xylohexaose. The CD appears to bind carboxymethylcellulose (CMC) and xylan only weakly, while the CBM and the CD/CBM bind them much more strongly than they bind the cellooligosaccharides. CMC is bound to a much greater degree than is xylan. Association constants for the cellooligosaccharides are in the order CBM CD \u3c CD/CBM, while those on CMC and xylan are CD CBM CD/CBM. A synergistic effect was observed for the association constants of cellopentaose and cellohexaose with the CD/CBM when compared to the CD and CBM alone. Binding of all ligands by all three constructs is energetically favorable, enthalpy-driven, and subject to enthalpy–entropy compensation

Kinetic characterization of a glycoside hydrolase family 44 xyloglucanase/endoglucanase from Ruminococcus flavefaciens FD-1

Two forms of Ruminococcus flavefaciens FD-1 endoglucanase B, a member of glycoside hydrolase family 44, one with only a catalytic domain and the other with a catalytic domain and a carbohydrate binding domain (CBM), were produced. Both forms hydrolyzed cellotetraose, cellopentaose, cellohexaose, carboxymethylcellulose (CMC), birchwood and larchwood xylan, xyloglucan, lichenan, and Avicel but not cellobiose, cellotriose, mannan, or pullulan. Addition of the CBM increased catalytic efficiencies on both CMC and birchwood xylan but not on xyloglucan, and it decreased rates of cellopentaose and cellohexaose hydrolysis. Catalytic efficiencies were much higher on xyloglucan than on other polysaccharides. Hydrolysis rates increased with increasing cellooligosaccharide chain length. Cellotetraose hydrolysis yielded only cellotriose and glucose. Hydrolysis of cellopentaose gave large amounts of cellotetraose and glucose, somewhat more of the former than of the latter, and much smaller amounts of cellobiose and cellotriose. Cellohexaose hydrolysis yielded much more cellotetraose than cellobiose and small amounts of glucose and cellotriose, along with a low and transient amount of cellopentaose