Functional Characterization of a Jacalin-like Lectin Domain-Containing Protein in \u3ci\u3eArabidopsis thaliana\u3c/i\u3e

Jacalin-related lectins (JRLs) are proteins that bind carbohydrates. Functionally, JRLs are thought to be involved in a diverse array of functions including biotic stress defense, intracellular storage, abiotic stress response, and plant development. Recent Arabidopsis thaliana microarray data indicate that a Jacalin-like domain-containing protein (JLL1) exhibits highly root specific expression. Previous transcriptomic and proteomic studies indicate that JLL1 may have a dual role in planta as a biotic stress defense protein and in plant development. In order to better understand the physiological function of JLL1 in Arabidopsis, several different analyses were conducted examining its regulatory sequences, spatial expression, responsiveness to abiotic stress, and its impact on seed germination. The results of these investigations reveal that JLL1 exhibits high sequence similarity with two adjacent jacalin domain-containing proteins. The cis-regulatory elements within JLL1\u27s promoter region are largely associated with plant development and metabolism. The spatial expression of JLL1 was localized in the vascular-associated regions of the plant roots, leaf vasculature, and root tip (cap). RTPCR data indicate that JLL1 is negatively regulated during abiotic stress, and JLL1 mutant seeds exhibited delayed germination under abiotic stress conditions. Our data supports the assertion that JLL1 has a dual role in planta as a protein involved in hormone-mediated early plant development, and as a secreted non-specific defense protein

Founders of Fish Culture

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141727/1/naaq0026.pd

Improvement of Fishing through a Knowledge of the Breeding Habits of Fishes

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141406/1/tafs0097.pd

Fanny E. Langdon

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Validation of the Compressed Assessment of Ability Related to Vision (CAARV), a Performance Based Measure, in a South Indian Population with Glaucoma

Medical Schoolhttps://deepblue.lib.umich.edu/bitstream/2027.42/148189/1/reighardc.pd

Adolescent grief: The nurse\u27s role in promoting healthy coping mechanisms and preventing future hospitalizations

Age and developmental stage directly influence the grieving individual\u27s ability to understand and process death. Adolescents are especially vulnerable to developing negative coping skills such as substance abuse, self-destructive behaviors, and eating disorders. After a loss occurs, nurses can use their role as a caregiver who is neither a parent nor a peer to provide adolescents with an opportunity to express their grief. Nurses can educate grieving adolescents on the differences between positive and negative coping skills and can therefore reduce the number of future hospitalizations related to ineffective coping mechanisms