Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion.

Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing two binding sites for the protein Fis. Two Hin dimers bound to specific recombination sites associate with the Fis-bound enhancer by DNA looping where they are remodeled into a synaptic tetramer competent for DNA chemistry and exchange. Here we show that the flexible beta-hairpin arms of the Fis dimers contact the DNA binding domain of one subunit of each Hin dimer. These contacts sandwich the Hin dimers to promote remodeling into the tetramer. A basic region on the Hin catalytic domain then contacts enhancer DNA to complete assembly of the active Hin tetramer. Our results reveal how the enhancer generates the recombination complex that specifies DNA inversion and regulates DNA exchange by the subunit rotation mechanism. DOI:http://dx.doi.org/10.7554/eLife.01211.001

Assembly of a Tightly Interwound DNA Recombination Complex Poised for Deletion

In a recent issue of Molecular Cell, Mouw et al. (2008) report a crystal structure of a serine recombinase bound to a regulatory DNA site in an unexpected synaptic complex configuration, which forms the framework for a new model of the entire 12 subunit, 186 bp deletion complex

END SPLICE ASSEMBLY FOR BOX-BEAM GUARDRAIL AND TERMINAL SYSTEMIS

An end splice assembly for a box-beam guardrail and terminal system having a first stage rail element and a second stage rail element. The assembly has two major connecting components. Upper and lower bent plate channels and upper and lower channel splice plates. The channels and plates have side walls which extend laterally to one another when the two rail elements are mated. The channels and plates are fastened together to provide moment strength to the splice within the system

ENERGY ABSORBING GUARDRAIL SYSTEM

A highway crash attenuation system having W - beam rail elements attached to a plurality of vertical posts . An impact terminal with a feeder chute guides one or more of the W - beam rail elements through the impact terminal . The feeder chute has an impact shield extending along a traffic facing side of the chute from an upstream - most end to a downstream - most end of the chute closing the traffic - facing side of the chute . The system also has an anchor cable release mechanism for releasing the cable downstream of the first vertical post and an improved first breakaway post

Deck-Mounted Steel Post Barrier System

An existing mountable safety barrier system, previously crash tested successfully on a wood bridge deck, was evaluated for use on a fiber reinforced plastic (FRP) bridge deck. In an attempt to avoid expensive full-scale crash testing, components of the existing system were evaluated using worst case conditions on two dynamic bogie crash tests and a series of computer simulations using nonlinear finite-element analysis. Simulation results closely approximated the physical results, with both displaying similar deformation, damage, and force levels. Both testing and simulation demonstrated that the barrier should function sufficiently if used on the FRP deck system. Further, the development of an accurate model makes it possible to evaluate the potential success of the existing system for use on other bridge decks. As an example, a more rigid bridge deck, similar to reinforced concrete, was evaluated. Results showed that due to the stiffer deck, more of the impact energy must be absorbed by the posts and attachment hardware, resulting in significantly more deformation than when used on the flexible FRP deck

Deck-Mounted Steel Post Barrier System

An existing mountable safety barrier system, previously crash tested successfully on a wood bridge deck, was evaluated for use on a fiber reinforced plastic (FRP) bridge deck. In an attempt to avoid expensive full-scale crash testing, components of the existing system were evaluated using worst case conditions on two dynamic bogie crash tests and a series of computer simulations using nonlinear finite-element analysis. Simulation results closely approximated the physical results, with both displaying similar deformation, damage, and force levels. Both testing and simulation demonstrated that the barrier should function sufficiently if used on the FRP deck system. Further, the development of an accurate model makes it possible to evaluate the potential success of the existing system for use on other bridge decks. As an example, a more rigid bridge deck, similar to reinforced concrete, was evaluated. Results showed that due to the stiffer deck, more of the impact energy must be absorbed by the posts and attachment hardware, resulting in significantly more deformation than when used on the flexible FRP deck

EVALUATION OF AN EXISTING STEEL POST ALTERNATIVE FOR THE THRIE BEAM BULLNOSE GUARDRAIL SYSTEM

Recently, the Minnesota Department of Transportation (MnDOT) funded a research project through the Midwest States Regional Pooled Fund to evaluate an existing steel post alternative for the thrie beam bullnose barrier system previously developed at the Midwest Roadside Safety Facility (MwRSF). MnDOT had an interest in the replacement of the wooden breakaway posts used in the current bullnose system with proprietary breakaway steel posts. The research project consisted of evaluation of current breakaway steel post designs, investigation and selection of a candidate post design, and full-scale testing of the bullnose system with a steel post alternative. The full-scale testing was to consist of two tests conducted according to the evaluation criteria of NCHRP Report 350: 1) Test 3-38, an impact of a 2000P vehicle on the Critical Impact Point (CIP) of the system at a speed of 100 km/h and an angle of 20 degrees, and 2) Test 3-31, an impact of a 2000P vehicle with the center of the vehicle aligned with the center of the nose of the system at a speed of 100 km/h and an angle of 0 degrees. The evaluation of the steel post alternative for the bullnose system project has been completed. A steel post alternative was selected followed by two full-scale crash tests. Unfortunately, both crash tests failed as the vehicle in each test ramped up the guardrail and vaulted the system. This letter summarizes the work completed

A Flaring L5 Dwarf: The Nature of H\alpha Emission in Very Low Mass (Sub)Stellar Objects

Time series spectrophotometry of the L5 dwarf 2MASS 01443536-0716142 showed strong H\alpha emission which declined by nearly 75% in four consecutive exposures. The line was not detected in emission on a spectrum obtained eleven months later. This behavior constrasts with that of 2MASSI J1315309-264951, an L5 dwarf which has shown even stronger H\alpha emission on four separate occasions. The observational database suggests that L dwarfs can be found in such strong flares only occasionally, with a duty cycle of order 1%. In contrast, the few, continuously-strong H\alpha emitters, including PC 0025+0447 and 2MASSI J1237392+652615, must either be (1) objects no older than 10-100 Myrs with continuously-active accretion and/or chromospheres, but which apparently formed in isolation from known young stellar clusters and associations, or (2) objects empowered by a different and unknown mechanism for the H\alpha energy.Comment: 11 pages, 2 figures, Astronomical Journal in press -- Jan 2003 issu