Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy

Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγ and inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs' expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Estudios Inmunológicos y Fisiopatológico

Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy

Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγ and inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs' expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Estudios Inmunológicos y Fisiopatológico

Domain-wall structure in thin films with perpendicular anisotropy: Magnetic force microscopy and polarized neutron reflectometry study

Ferromagnetic domain patterns and three-dimensional domain-wall configurations in thin CoCrPt films with perpendicular magnetic anisotropy were studied in detail by combining magnetic force microscopy and polarized neutron reflectometry with micromagnetic simulations. With the first method, lateral dimension of domains with alternative magnetization directions normal to the surface and separated by domain walls in 20-nm-thick CoCrPt films were determined in good agreement with micromagnetic simulations. Quantitative analysis of data on reflectometry shows that domain walls consist of a Bloch wall in the center of the thin film, which is gradually transformed into a pair of Néel caps at the surfaces. The width and in-depth thickness of the Bloch wall element, transition region, and Néel caps are found consistent with micromagnetic calculations. A complex structure of domain walls serves to compromise a competition between exchange interactions, keeping spins parallel, magnetic anisotropy orienting magnetization normal to the surface, and demagnetizing fields, promoting in-plane magnetization. It is shown that the result of such competition strongly depends on the film thickness, and in the thinner CoCrPt film (10 nm thick), simple Bloch walls separate domains. Their lateral dimensions estimated from neutron scattering experiments agree with micromagnetic simulations.Microelectronics Advanced Research Corporation (MARCO)United States. Defense Advanced Research Projects Agenc

Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy

Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγ and inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs' expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Estudios Inmunológicos y Fisiopatológico

Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy

Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγ and inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs’ expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa

Domain-wall structure in thin films with perpendicular anisotropy: Magnetic force microscopy and polarized neutron reflectometry study

Ferromagnetic domain patterns and three-dimensional domain-wall configurations in thin CoCrPt films with perpendicular magnetic anisotropy were studied in detail by combining magnetic force microscopy and polarized neutron reflectometry with micromagnetic simulations. With the first method, lateral dimension of domains with alternative magnetization directions normal to the surface and separated by domain walls in 20-nm-thick CoCrPt films were determined in good agreement with micromagnetic simulations. Quantitative analysis of data on reflectometry shows that domain walls consist of a Bloch wall in the center of the thin film, which is gradually transformed into a pair of Néel caps at the surfaces. The width and in-depth thickness of the Bloch wall element, transition region, and Néel caps are found consistent with micromagnetic calculations. A complex structure of domain walls serves to compromise a competition between exchange interactions, keeping spins parallel, magnetic anisotropy orienting magnetization normal to the surface, and demagnetizing fields, promoting in-plane magnetization. It is shown that the result of such competition strongly depends on the film thickness, and in the thinner CoCrPt film (10 nm thick), simple Bloch walls separate domains. Their lateral dimensions estimated from neutron scattering experiments agree with micromagnetic simulations.D.N. thanks the Ministerio de Economia y Competitividad (MAT2010-20798-C05-02) and Fundaçao para a Ciência e Tecnologia (Grant No. SFRH/BPD/89808/2012) for financial support and the Institut Laue-Langevin for providing beam time for the PNR experiment and technical support. C.A.R. acknowledges support of C-SPIN, one of six STARnet centers of Microelectronics Advanced Research Corporation (MARCO) and Defense Advanced Research Projects Agency (DARPA)

Proyecto de Prevención de Drogodependencias

El proyecto tiene como finalidad prevenir la aparición de problemas con el consumo de drogas entre los miembros de la comunidad educativa. Los objetivos son promocionar la salud y educar a los alumnos para la utilización positiva del tiempo de ocio ofreciéndoles alternativas satisfactorias. Los principios metodológicos se basan en mantener una actitud activa, participativa, dialogante y globalizadora. Las actividades son deportivas, de teatro, debates, juegos de roles, juegos compartidos, encuestas, celebración de la Semana de la Salud y análisis de publicidad. La valoración está referida al diseño de la programación, al desarrollo de las propuestas didácticas de las áreas, a los recursos materiales y a la incidencia en la dinámica escolar, en el alumnado y en la comunidad.Madrid (Comunidad Autónoma). Consejería de Educación y Cultura. Ministerio de Educación y Cultura. Ayuntamiento de MadridMadridMadrid (Comunidad Autónoma). Subdirección General de Formación del Profesorado. CRIF Las Acacias; General Ricardos 179 - 28025 Madrid; Tel. + 34915250893ES

Propuesta de actividades para la estimulación del lenguaje oral en educación infantil

Los autores son todos miembros de los Equipos de Orientación Educativa de Córdoba. Anexos en las páginas 108-123Esta publicación es el resultado del trabajo continuado que vienen desarrollando los maestros y las maestras de Audición y Lenguaje de los Equipos de Orientación Educativa de Córdoba, y que se concreta en la práctica educativa con la aplicación del programa de Prevención de Dificultades en el Lenguaje Oral ofertado a los centros escolares y realizado junto con el profesorado de Educación Infantil. El lenguaje oral está presente en la comunicación de las personas desde el mismo momento del nacimiento, por ello la familia y el entorno más cercano serán los pilares que proporcionen las primeras experiencias para que el lenguaje sea vivo y variado. El interés de esta obra se centra en que el alumnado de Educación Infantil continúe adquiriendo de la forma más adecuada el lenguaje oral en todas sus dimensiones, conscientes de la importancia que su correcta adquisición tiene para el desarrollo personal. Basado en un modelo preventivo, el documento que se propone tiene como finalidad enriquecer la competencia lingüística del alumnado de esta etapa, y facilitar la superación de las posibles dificultades del lenguaje que se detecten. El material está estructurado en distintos apartados que se complementan y relacionan entre sí para posibilitar su integración en la programación de aula. Es un documento abierto que permite generar otras actividades y que sin duda el profesorado enriquecerá con sus aportaciones fruto del análisis y reflexión sobre la práctica docente.AndalucíaInstituto Psicopedagógico Dulce Nombre de María (Málaga); Calle Manuel de Palacio, 17; 29017 Málaga; +34902290499; [email protected]

Integración de los alumnos en la gestión y desarrollo de los laboratorios de ciencias

Depto. de Didáctica de las Ciencias Experimentales , Sociales y MatemáticasFac. de EducaciónFALSEsubmitte