Brazilian version of the Clinical COPD Questionnaire, administered by interview:reliability and validity measurement properties

OBJECTIVE: To test the reliability, validity, and interpretability of the Brazilian version of the Clinical COPD Questionnaire (CCQ) in patients with COPD. METHODS: Fifty patients with COPD completed the CCQ by interview on two occasions. At the first visit, the CCQ was administered twice, by two different raters, approximately 10 min apart; the patients also underwent spirometry and were administered the COPD Assessment Test, the modified Medical Research Council scale, and Saint George’s Respiratory Questionnaire (SGRQ). At the second visit (1-2 weeks later), the CCQ was readministered. We tested the hypothesis that the CCQ total score would correlate positively with the total and domain SGRQ scores (r ≥ 0.5). RESULTS: Of the 50 patients, 30 (60%) were male. The mean age was 66 ± 8 years, and the mean FEV(1) was 44.7 ± 17.9% of the predicted value. For all CCQ items, Cronbach’s alpha coefficient (95% CI) was 0.93 (0.91-0.96). To analyze the interrater reliability and test-retest reliability of the CCQ, we calculated the two-way mixed effects model/single measure type intraclass correlation coefficient (0.97 [95% CI: 0.95-0.98] and 0.92 [95% CI: 0.86-0.95], respectively); the agreement standard error of measurement (0.65 for both); the smallest detectable change at the individual level (1.81 and 1.80, respectively) and group level (0.26 and 0.25, respectively); and the limits of agreement (−0.58 to 0.82 and −1.14 to 1.33, respectively). The CCQ total score correlated positively with all SGRQ scores (r ≥ 0.70 for all). CONCLUSIONS: The Brazilian version of the CCQ showed an indeterminate measurement error, as well as satisfactory interrater/test-retest reliability and construct validity

Hacia la inclusión digital y equidad educativa: una evaluación integral de los materiales curriculares utilizados en las áreas de gramática y fonética inglesas y lectocomprensión en inglés

Sin lugar a dudas, en el esquema pedagógico-didáctico, los materiales curriculares cumplen una seriede funciones que surgen de la interacción entre el docente, el estudiante y el currículum. En virtud deque los materiales contribuyen a que los estudiantes logren el dominio de un contenido teóricoprácticodeterminado y adquieran ciertas habilidades, destrezas y estrategias de aprendizaje, suimportancia es incuestionable en un escenario mediado por las tecnologías digitales como el quehemos transitado durante los ciclos lectivos 2020 y 2021. En este contexto, creemos que evaluar losmateriales curriculares es imperioso para mejorar las prácticas educativas, más aún cuando estosmateriales son diseñados por los propios docentes. Cuando hablamos de diseño, nos referimos a laidea de que, cualquiera sea el contexto en el cual se va a desarrollar la acción formativa, el docentedebe tener en claro a quiénes está dirigida, qué pretende con esa acción y de qué manera va arealizarla. Ahora bien, para optimizar la calidad de los procesos de enseñanza y de aprendizaje ygarantizar equidad e igualdad de oportunidades entre los estudiantes, los materiales curricularesdeben cumplir con una serie de requisitos, por lo cual, es necesario aplicar criterios e indicadores deanálisis y valoración para evaluar su calidad. Este trabajo se desprende de un proyecto de investigaciónmayor que estamos desarrollando en la Facultad de Lenguas de la Universidad Nacional de Córdoba.En esta oportunidad, expondremos acerca de los resultados preliminares de nuestro estudio en cuatroasignaturas específicas: Práctica Gramatical del Inglés, Gramática Inglesa I, Práctica de laPronunciación del Inglés y Lectocomprensión en Lengua Extranjera IV (Inglés)

Anticipating the Flood. Community-based cartography for disaster flood events in Argentina

Anticipando la Crecida Project (Anticipating the Flood) is an interdisciplinary project which deals with flood risk management strategies associated with intense rain events and southeasterly wind « sudestadas » in socio-economical vulnerable urban areas in Argentina. The objective of the current study is to use local knowledge through participatory activities to strengthen the phase of risk awareness of an early warning system by using cartography as a work tool. For this purpose, eleven workshops with adults and children were held between 2014 and 2017 in Buenos Aires metropolitan area and the towns of San Antonio de Areco and Santa Lucía. By helping communities articulate and communicate spatial knowledge through workshops, enable the possibility to advocate for a change. That change result in a new direction on how the community and stakeholders can act towards a flood event. Analysing community-based maps of 85 adults, flood theoretical models can be evaluated and perhaps improved. Additionally, overall 287 students between 10 and 17 years old learned about cartography and interacted with poster maps during the workshops held in different schoolsFil: Gatti, Ignacio Agustin. Universidad de Buenos Aires. Facultad de Filosofía y Letras; ArgentinaFil: Robledo, Federico Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias de la Atmósfera y los Océanos; ArgentinaFil: Hurtado, Sol. Universidad Nacional de San Martín; Argentina. Universidad de Buenos Aires; ArgentinaFil: Canneva, Julieta. Universidad Nacional de San Martín. Instituto de Altos Estudios Sociales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moreira, Diego. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias de la Atmósfera y los Océanos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Re, Mariano. Ministerio del Interior, Obras Públicas y Vivienda. Secretaría de Obras Públicas. Subsecretaría de Recursos Hídricos. Instituto Nacional del Agua y del Ambiente (Ezeiza); ArgentinaFil: Briche, Elodie Sylvie Odile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Falco, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Kazimierski, Leandro David. Ministerio del Interior, Obras Públicas y Vivienda. Secretaría de Obras Públicas. Subsecretaría de Recursos Hídricos. Instituto Nacional del Agua y del Ambiente (Ezeiza); ArgentinaFil: Micou, Ana Paula. Ministerio de Defensa. Instituto Geografico Nacional; Argentin

Manejo pre, intra y pos-quirúrgico de dos caninos cardiópatas con fibrilación atrial

Las enfermedades cardiovasculares disparan mecanismos compensatorios fisiológicos y neurohumorales que modifican la función cardíaca y pulmonar, influyendo en los efectos de la anestesia. Sumado a esto, todos los agentes anestésicos deprimen la función cardiovascular directa o indirectamente. No existe un protocolo anestésico único e ideal para todos los pacientes con enfermedad cardíaca. El objetivo de este trabajo es presentar el manejo pre, intra y pos quirúrgico de dos pacientes con insuficiencia cardíaca congestiva compensada y fibrilación atrial sometidos a cirugía menor, no cardíaca.Facultad de Ciencias Veterinaria

Proliferation and survival molecules implicated in the inhibition of BRAF pathway in thyroid cancer cells harbouring different genetic mutations

<p>Abstract</p> <p>Background</p> <p>Thyroid carcinomas show a high prevalence of mutations in the oncogene BRAF which are inversely associated with RAS or RET/PTC oncogenic activation. The possibility of using inhibitors on the BRAF pathway as became an interesting therapeutic approach. In thyroid cancer cells the target molecules, implicated on the cellular effects, mediated by inhibition of BRAF are not well established. In order to fill this lack of knowledge we studied the proliferation and survival pathways and associated molecules induced by BRAF inhibition in thyroid carcinoma cell lines harbouring distinct genetic backgrounds.</p> <p>Methods</p> <p>Suppression of BRAF pathway in thyroid cancer cell lines (8505C, TPC1 and C643) was achieved using RNA interference (RNAi) for BRAF and the kinase inhibitor, sorafenib. Proliferation analysis was performed by BrdU incorporation and apoptosis was accessed by TUNEL assay. Levels of protein expression were analysed by western-blot.</p> <p>Results</p> <p>Both BRAF RNAi and sorafenib inhibited proliferation in all the cell lines independently of the genetic background, mostly in cells with BRAF^V600Emutation. In BRAF^V600Emutated cells inhibition of BRAF pathway lead to a decrease in ERK1/2 phosphorylation and cyclin D1 levels and an increase in p27^Kip1. Specific inhibition of BRAF by RNAi in cells with BRAF^V600Emutation had no effect on apoptosis. In the case of sorafenib treatment, cells harbouring BRAF^V600Emutation showed increase levels of apoptosis due to a balance of the anti-apoptotic proteins Mcl-1 and Bcl-2.</p> <p>Conclusion</p> <p>Our results in thyroid cancer cells, namely those harbouring BRAF^V600Emutation showed that BRAF signalling pathway provides important proliferation signals. We have shown that in thyroid cancer cells sorafenib induces apoptosis by affecting Mcl-1 and Bcl-2 in BRAF^V600Emutated cells which was independent of BRAF. These results suggest that sorafenib may prove useful in the treatment of thyroid carcinomas, particularly those refractory to conventional treatment and harbouring BRAF mutations.</p

Prolonged Survival of Allografts Induced by Mycobacterial Hsp70 Is Dependent on CD4+CD25+ Regulatory T Cells

Background: Heat shock proteins (Hsps) are stress induced proteins with immunomodulatory properties. The Hsp70 of Mycobacterium tuberculosis (TBHsp70) has been shown to have an anti-inflammatory role on rodent autoimmune arthritis models, and the protective effects were demonstrated to be dependent on interleukin-10 (IL-10). We have previously observed that TBHsp70 inhibited maturation of dendritic cells (DCs) and induced IL-10 production by these cells, as well as in synovial fluid cells. Methodology/Principal Findings: We investigated if TBHsp70 could inhibit allograft rejection in two murine allograft systems, a transplanted allogeneic melanoma and a regular skin allograft. In both systems, treatment with TBHsp70 significantly inhibited rejection of the graft, and correlated with regulatory T cells (Tregs) recruitment. This effect was not tumor mediated because injection of TBHsp70 in tumor-free mice induced an increase of Tregs in the draining lymph nodes as well as inhibition of proliferation of lymph node T cells and an increase in IL-10 production. Finally, TBHsp70 inhibited skin allograft acute rejection, and depletion of Tregs using a monoclonal antibody completely abolished this effect. Conclusions/Significance: We present the first evidence for an immunosuppressive role for this protein in a graft rejection system, using an innovative approach - immersion of the graft tissue in TBHsp70 solution instead of protein injection. Also, this is the first study that demonstrates dependence on Treg cells for the immunosuppressive role of TBHsp70. This finding is relevant for the elucidation of the immunomodulatory mechanism of TBHsp70. We propose that this protein can be used not only for chronic inflammatory diseases, but is also useful for organ transplantation management.Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Financiadora de Estudos e Projetos (FINEP

Endocytosis of DNA-Hsp65 Alters the pH of the Late Endosome/Lysosome and Interferes with Antigen Presentation

BACKGROUND: Experimental models using DNA vaccine has shown that this vaccine is efficient in generating humoral and cellular immune responses to a wide variety of DNA-derived antigens. Despite the progress in DNA vaccine development, the intracellular transport and fate of naked plasmid DNA in eukaryotic cells is poorly understood, and need to be clarified in order to facilitate the development of novel vectors and vaccine strategies. METHODOLOGY AND PRINCIPAL FINDINGS: Using confocal microscopy, we have demonstrated for the first time that after plasmid DNA uptake an inhibition of the acidification of the lysosomal compartment occurs. This lack of acidification impaired antigen presentation to CD4 T cells, but did not alter the recruitment of MyD88. The recruitment of Rab 5 and Lamp I were also altered since we were not able to co-localize plasmid DNA with Rab 5 and Lamp I in early endosomes and late endosomes/lysosomes, respectively. Furthermore, we observed that the DNA capture process in macrophages was by clathrin-mediated endocytosis. In addition, we observed that plasmid DNA remains in vesicles until it is in a juxtanuclear location, suggesting that the plasmid does not escape into the cytoplasmic compartment. CONCLUSIONS AND SIGNIFICANCE: Taken together our data suggests a novel mechanism involved in the intracellular trafficking of plasmid DNA, and opens new possibilities for the use of lower doses of plasmid DNA to regulate the immune response