ASSESSMENT OF THE ECONOMIC POTENTIAL OF THE ENTERPRISE

Purpose of the study: The article deals with the problems of forming and increasing the efficiency of using the basic production assets of an enterprise. Methodology: Methods of working with physically challenged children (PCC) are described in the works by V .V.Linkov, N .N. Malofeev, N. M. Nazarov, etc. They consider various methods and techniques of organizing educational work with children having health problems. Results: The analysis of the effectiveness of the formation and use of the basic production assets of a machine-building enterprise has been carried out, and measures have been considered to improve their formation and use. Applications of this study: This research can be used for the universities, teachers, and students. Novelty/Originality of this study: In this research, the model of assessment of the economic potential of the enterprise is presented in a comprehensive and complete manner

Financial analysis of the leading oil and gas companies in the Russian Federation for 2021

Oil and gas complex is a common name for a group of industries for the extraction, transportation and processing of oil and gas and the distribution of products of their processing. Oil and gas complex plays a significant role in the development of the economy both in the domestic and foreign markets. The article focuses on the contribution of the industry to the country’s economy, in addition, the resource base of minerals of the Russian Federation is considered. The dependence of the Russian economy on oil and gas is clearly demonstrated. The analysis of the current situation on the oil and gas market is carried out, the impact of the oil and gas complex on the economic indicators of Russia is assessed. The article examines and analyses the leading vertically integrated oil companies. This analysis allows us to see the developing and increasing indicators of companies in terms of progress

Information Potential of the Collection of Documents «Professional-Pedagogical and Professional-Technical Education in Russia (1920-2020)»

В статье рассматривается информационный потенциал трехтомного сборника архивных документов по истории профессионально-технического и профессионально-педагогического образования, подготовленного преподавателями РГППУ.The article examines the information potential of a three-volume collection of archival documents on the history of vocational and vocational pedagogical education, prepared by the teachers of the Russian State Pedagogical University

Hemifusion between cells expressing hemagglutinin of influenza virus and planar membranes can precede the formation of fusion pores that subsequently fully enlarge.

The chronological relation between the establishment of lipid continuity and fusion pore formation has been investigated for fusion of cells expressing hemagglutinin (HA) of influenza virus to planar bilayer membranes. Self-quenching concentrations of lipid dye were placed in the planar membrane to monitor lipid mixing, and time-resolved admittance measurements were used to measure fusion pores. For rhodamine-PE, fusion pores always occurred before a detectable amount of dye moved into an HA-expressing cell. However, with DiI in the planar membrane, the relationship was reversed: the spread of dye preceded formation of small pores. In other words, by using DiI as probe, hemifusion was clearly observed to occur before pore formation. For hemifused cells, a small pore could form and subsequently fully enlarge. In contrast, for cells that express a glycosylphosphatidylinositol-anchored ectodomain of HA, hemifusion occurred, but no fully enlarged pores were observed. Therefore, the transmembrane domain of HA is required for the formation of fully enlarging pores. Thus, with the planar bilayer membranes as target, hemifusion can precede pore formation, and the occurrence of lipid dye spread does not preclude formation of pores that can enlarge fully

Effects of Membrane Potential and Sphingolipid Structures on Fusion of Semliki Forest Virus

Cells expressing the E1 and E2 envelope proteins of Semliki Forest virus (SFV) were fused to voltage-clamped planar lipid bilayer membranes at low pH. Formation and evolution of fusion pores were electrically monitored by capacitance measurements, and membrane continuity was tracked by video fluorescence microscopy by including rhodamine-phosphatidylethanolamine in the bilayer. Fusion occurred without leakage for a negative potential applied to the trans side of the planar membrane. When a positive potential was applied, leakage was severe, obscuring the observation of any fusion. E1-mediated cell-cell fusion occurred without leakage for negative intracellular potentials but with substantial leakage for zero membrane potential. Thus, negative membrane potentials are generally required for nonleaky fusion. With planar bilayers as the target, the first fusion pore that formed almost always enlarged; pore flickering was a rare event. Similar to other target membranes, fusion required cholesterol and sphingolipids in the planar membrane. Sphingosine did not support fusion, but both ceramide, with even a minimal acyl chain (C(2)-ceramide), and lysosphingomyelin (lyso-SM) promoted fusion with the same kinetics. Thus, unrelated modifications to different parts of sphingosine yielded sphingolipids that supported fusion to the same degree. Fusion studies of pyrene-labeled SFV with cholesterol-containing liposomes showed that C(2)-ceramide supported fusion while lyso-SM did not, apparently due to its positive curvature effects. A model is proposed in which the hydroxyls of C-1 and C-3 as well as N of C-2 of the sphingosine backbone must orient so as to form multiple hydrogen bonds to amino acids of SFV E1 for fusion to proceed

Osmolyte Effects on Monoclonal Antibody Stability and Concentration-Dependent Protein Interactions with Water and Common Osmolytes

Preferential interactions of proteins with water and osmolytes play a major role in controlling the thermodynamics of protein solutions. While changes in protein stability and shifts in phase behavior are often reported with the addition of osmolytes, the underlying protein interactions with water and/or osmolytes are typically inferred rather than measured directly. In this work, Kirkwood–Buff integrals for protein–water interactions (<i>G</i>₁₂) and protein–osmolyte interactions (<i>G</i>₂₃) were determined as a function of osmolyte concentration from density measurements of antistreptavidin immunoglobulin gamma-1 (AS-IgG1) in ternary aqueous solutions for a set of common neutral osmolytes: sucrose, trehalose, sorbitol, and poly­(ethylene glycol) (PEG). For sucrose and PEG solutions, both protein–water and protein–osmolyte interactions depend strongly on osmolyte concentrations (<i>c</i>₃). Strikingly, both osmolytes change from being preferentially excluded to preferentially accumulated with increasing <i>c</i>₃. In contrast, sorbitol and trehalose solutions do not show large enough preferential interactions to be detected by densimetry. <i>G</i>₁₂ and <i>G</i>₂₃ values are used to estimate the transfer free energy for native AS-IgG1 (Δμ₂^<i>N</i>) and compared with existing models. AS-IgG1 unfolding via calorimetry shows a linear increase in midpoint temperatures as a function of trehalose, sucrose, and sorbitol concentrations, but the opposite behavior for PEG. Together, the results highlight limitations of existing models and common assumptions regarding the mechanisms of protein stabilization by osmolytes. Finally, PEG preferential interactions destabilize the Fab regions of AS-IgG1 more so than the C_H2 or C_H3 domains, illustrating preferential interactions can be specific to different protein domains

Thermoelectric characterization of the clathrate-I solid solution Ba_8-δAu_xGe_46-x

Clathrate-I-based materials are promising for waste-heat recovering applications via thermoelectric (TE) effects. However, the lack of highly efficient p-type materials hampers the development of clathrate-based TE devices. In this work, the synthesis of the p-type semiconductor Ba7.8Au5.33Ge40.67 with clathrate-I structure is up-scaled by steel-quenching and spark plasma sintering treatment at 1073 K. A thermoelectric figure of merit ZT approximate to 0.9 at 670 K is reproducibly obtained, and 40 chemically homogeneous module legs of 5 x 5 x 7 mm(3) are fabricated. By using a carbon layer as a diffusion barrier, electrical contacts are sustainable at elevated application temperatures. Eight couples with the clathrate-I compounds Ba7.8Au5.33Ge40.67 as p-type and Ba8Ga16Ge30 as n-type materials are integrated into a TE module with an output power of 0.2 W achieved under a temperature difference Delta T = 380 K (T-1 = 673 K and T-2 = 293 K). The thermoelectric performance of Ba7.8Au5.33Ge40.67 demonstrates the potential of type-I clathrates for waste heat recycling

Mass distributions of the system 136Xe + 208Pb at laboratory energies around the Coulomb barrier:A candidate reaction for the production of neutron-rich nuclei at N = 126

Reaction products from the system 136Xe + 208Pb at 136Xe ions laboratory energies of 700, 870, and 1020 MeV were studied by two-body kinematics and by a catcher-foil activity analysis to explore the theoretically proposed suitability of such reaction as a means to produce neutron-rich nuclei in the neutron shell closure N = 126. Cross sections for products heavier than 208Pb were measured and were found sensibly larger than new theoretical predictions. Transfers of up to 16 nucleons from Xe to Pb were observed