Characterizing small-scale migration behavior of sequestered CO2 in a realistic geological fabric

For typical reservoir conditions, buoyancy and capillary forces grow dominant over viscous forces within a few hundred meters of the injection wells as the pressure gradient due to injection decreases, resulting in qualitatively different plume migration regimes. The migration regime depends on two factors: the capillary pressure of the leading edge of the plume and the range of threshold entry pressures within the rock at the leading edge of the plume. A capillary channel regime arises when these two factors have the same magnitude. Flow patterns within this regime vary from finger-like structures with minimal rock contact to back-filling structures with compact volumes of saturation distributed between fingers. Reservoir heterogeneity is one of the principal factors influencing CO2 migration pathway in the capillary channel regime. Here we characterize buoyancy-driven migration in a natural 2D geologic domain (1 m × 0.5 m peel from an alluvium) in which sedimentologic heterogeneity has been resolved at sub-millimeter (depositional) resolution. The relevant features of the heterogeneity are grain size distribution, which determines the mean and range of threshold pressures and correlation lengths of threshold pressures in horizontal and vertical directions. The relevant physics for this migration regime is invasion percolation, and simulations indicate that CO2 migrates through the peel in a few narrow pathways which cannot be captured by conventional coarse-grid simulations. The storage efficiency of the capillary channel regime would be low and consequently CO2 would also migrate greater distances than expected from models or simulations that neglect the capillary channel flow regime.Bureau of Economic Geolog

Radiological and Nuclear Emergencies: Medical Management of Radiation Injuries

Nuclear radiation which could be in the form of alpha, beta, gamma rays, etc, could cause radioactive contamination, radiation burns, acute radiation syndrome or a combination of any of these above-mentioned disasters. Effects of radiation and the subsequent treatment depend on the severity of exposure and the organs directly involved. Radiation levels up to 200 rads lead to nausea and vomiting whilst radiation levels between 200 rads and 400 rads lead to diarrhea, vomiting and pneumonitis. Whilst 450 rads is lethal in 50 per cent population, doses above this cause increased fatality and organ involvement with the Central Nervous System being affected with 2000 rads radiation. Nuclear disaster management lies most importantly in identifying that patient who would recover if treated immediately. Whereas decontamination of skin and wounds is done first, immediate first aid may take priority in a seriously injured patient. In the event of internal contamination, effective decorporation maybe required. This is followed by prevention and treatment of infections in sterile conditions. Radiation burn injuries will require effective long-term management. Finally, what would be most important is the necessity to have suitable hospital care where bone marrow, stem cell transfusion and restitution of the immune system would take place.Defence Science Journal, 2011, 61(2), pp.113-117, DOI:http://dx.doi.org/10.14429/dsj.61.83

Sec6 mutations and the Drosophila exocyst complex

To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function

Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii.

Correct folding and post-translational modifications are vital for therapeutic proteins to elicit their biological functions. Osteopontin (OPN), a bone regenerative protein present in a range of mammalian cells, is an acidic phosphoprotein with multiple potential phosphorylation sites. In this study, the ability of unicellular microalgae, Chlamydomonas reinhardtii, to produce phosphorylated recombinant OPN in its chloroplast is investigated. This study further explores the impact of phosphorylation and expression from a "plant-like" algae on separation of OPN. Chromatography resins ceramic hydroxyapatite (CHT) and Gallium-immobilized metal affinity chromatography (Ga-IMAC) were assessed for their binding specificity to phosphoproteins. Non-phosphorylated recombinant OPN expressed in E. coli was used to compare the specificity of interaction of the resins to phosphorylated OPN. We observed that CHT binds OPN by multimodal interactions and was better able to distinguish phosphorylated proteins in the presence of 250 mM NaCl. Ga-IMAC interaction with OPN was not selective to phosphorylation, irrespective of salt, as the resin bound OPN from both algal and bacterial sources. Anion exchange chromatography proved an efficient capture method to partially separate major phosphorylated host cell protein impurities such as Rubisco from OPN

The PPARγ Agonist Rosiglitazone Suppresses Syngeneic Mouse SCC (Squamous Cell Carcinoma) Tumor Growth through an Immune-Mediated Mechanism

Recent evidence suggests that PPARγ agonists may promote anti-tumor immunity. We show that immunogenic PDV cutaneous squamous cell carcinoma (CSCC) tumors are rejected when injected intradermally at a low cell number (1 × 106) into immune competent syngeneic hosts, but not immune deficient mice. At higher cell numbers (5 × 106 PDV cells), progressively growing tumors were established in 14 of 15 vehicle treated mice while treatment of mice with the PPARγ agonist rosiglitazone resulted in increased tumor rejection (5 of 14 tumors), a significant decrease in PDV tumor size, and a significant decrease in tumor cell Ki67 labeling. Rosiglitazone treatment had no effect on tumor rejection, tumor volume or PDV tumor cell proliferation in immune deficient NOD.CB17-PrkdcSCID/J mice. Rosiglitazone treatment also promoted an increase in tumor infiltrating CD3+ T-cells at both early and late time points. In contrast, rosiglitazone treatment had no significant effect on myeloid cells expressing either CD11b or Gr-1 but suppressed a late accumulation of myeloid cells expressing both CD11b and Gr-1, suggesting a potential role for CD11b+Gr-1+ myeloid cells in the late anti-tumor immune response. Overall, our data provides evidence that the PPARγ agonist rosiglitazone promotes immune-mediated anti-neoplastic activity against tumors derived from this immunogenic CSCC cell line

Environment and the cosmic evolution of star formation

We present a mark correlation analysis of the galaxies in the Sloan Digital Sky Survey using weights provided by MOPED. The large size of the sample permits statistically significant statements about how galaxies with different metallicities and star formation histories are spatially correlated. Massive objects formed a larger fraction of their stars at higher redshifts and over shorter timescales than did less massive objects (sometimes called down-sizing). We find that those galaxies which dominated the cosmic star formation at z~3 are predominantly in clusters today, whereas galaxies which dominate the star formation at z~0 inhabit substantially lower mass objects in less dense regions today. Hence, our results indicate that star formation and chemical enrichment occured first in the denser regions of the Universe, and moved to less dense regions at later times.Comment: 4 pages, 4 figures, submitted to ApJ