Stuttering Min oscillations within E. coli bacteria: A stochastic polymerization model

We have developed a 3D off-lattice stochastic polymerization model to study subcellular oscillation of Min proteins in the bacteria Escherichia coli, and used it to investigate the experimental phenomenon of Min oscillation stuttering. Stuttering was affected by the rate of immediate rebinding of MinE released from depolymerizing filament tips (processivity), protection of depolymerizing filament tips from MinD binding, and fragmentation of MinD filaments due to MinE. Each of processivity, protection, and fragmentation reduces stuttering, speeds oscillations, and reduces MinD filament lengths. Neither processivity or tip-protection were, on their own, sufficient to produce fast stutter-free oscillations. While filament fragmentation could, on its own, lead to fast oscillations with infrequent stuttering; high levels of fragmentation degraded oscillations. The infrequent stuttering observed in standard Min oscillations are consistent with short filaments of MinD, while we expect that mutants that exhibit higher stuttering frequencies will exhibit longer MinD filaments. Increased stuttering rate may be a useful diagnostic to find observable MinD polymerization in experimental conditions.Comment: 21 pages, 7 figures, missing unit for k_f inserte

The effect of interactions between a bacterial strain isolated from drinking water and a pathogen surrogate on biofilms formation diverged under static vs flow conditions

AimsInteractions with water bacteria affect the incorporation of pathogens into biofilms and thus pathogen control in drinking water systems. This study was to examine the impact of static vs flow conditions on interactions between a pathogen and a water bacterium on pathogen biofilm formation under laboratory settings.Methods and ResultsA pathogen surrogate Escherichia coli and a drinking water isolate Stenotrophomonas maltophilia was selected for this study. Biofilm growth was examined under two distinct conditions, in flow cells with continuous medium supply vs in static microtitre plates with batch culture. E. coli biofilm was greatly stimulated (c. 2–1000 times faster) with the presence of S. maltophilia in flow cells, but surprisingly inhibited (c. 65–95% less biomass) in microtitre plates. These divergent effects were explained through various aspects including surface attachment, cellular growth, extracellular signals and autoaggregation.ConclusionsInteractions with the same water bacterium resulted in different effects on E. coli biofilm formation when culture conditions changed from static to flow.Significance and Impact of StudyThis study highlights the complexity of species interactions on biofilm formation and suggests that environmental conditions such as the flow regime can be taken into consideration for the management of microbial contamination in drinking water systems.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140002/1/jam13596.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/140002/2/jam13596_am.pd

Dynamic compartmentalization of bacteria: accurate division in E. coli

Positioning of the midcell division plane within the bacterium E. coli is controlled by the min system of proteins: MinC, MinD and MinE. These proteins coherently oscillate from end to end of the bacterium. We present a reaction--diffusion model describing the diffusion of min proteins along the bacterium and their transfer between the cytoplasmic membrane and cytoplasm. Our model spontaneously generates protein oscillations in good agreement with experiments. We explore the oscillation stability, frequency and wavelength as a function of protein concentration and bacterial length.Comment: 4 pages, 4 figures, Latex2e, Revtex

The HERMES Solar Atlas and the spectroscopic analysis of the seismic solar analogue KIC3241581

Solar-analog stars provide an excellent opportunity to study the Sun's evolution, i.e. the changes with time in stellar structure, activity, or rotation for solar-like stars. The unparalleled photometric data from the NASA space telescope Kepler allows us to study and characterise solar-like stars through asteroseismology. We aim to spectroscopically investigate the fundamental parameter and chromospheric activity of solar analogues and twins, based on observations obtained with the HERMES spectrograph and combine them with asteroseismology. Therefore, we need to build a solar atlas for the spectrograph, to provide accurate calibrations of the spectroscopically determined abundances of solar and late type stars observed with this instrument and thus perform differential spectral comparisons. We acquire high-resolution and high signal-to-noise spectroscopy to construct three solar reference spectra by observing the reflected light of Vesta and Victoria asteroids and Europa (100<S/N<450) with the \Hermes spectrograph. We then observe the Kepler solar analog KIC3241581 (S/N~170). We constructed three solar spectrum atlases from 385 to 900 nm obtained with the Hermes spectrograph from observations of two bright asteroids and Europa. A comparison between our solar spectra atlas to the Kurucz and HARPS solar spectrum shows an excellent agreement. KIC3241581 was found to be a long-periodic binary system. The fundamental parameter for the stellar primary component are Teff=5689+/-11K, logg=4.385+/-0.005, [Fe/H]=+0.22+/-0.01, being in agreement with the published global seismic values confirming its status of solar analogue. KIC 3241581 is a metal rich solar analogue with a solar-like activity level in a binary system of unknown period. The chromospheric activity level is compatible to the solar magnetic activity.Comment: 12 pages, 8 figures, accepted for publication in A&

Teachers developing assessment for learning: impact on student achievement

While it is generally acknowledged that increased use of formative assessment (or assessment for learning) leads to higher quality learning, it is often claimed that the pressure in schools to improve the results achieved by students in externally-set tests and examinations precludes its use. This paper reports on the achievement of secondary school students who worked in classrooms where teachers made time to develop formative assessment strategies. A total of 24 teachers (2 science and 2 mathematics teachers, in each of six schools in two LEAs) were supported over a six-month period in exploring and planning their approach to formative assessment, and then, beginning in September 1999, the teachers put these plans into action with selected classes. In order to compute effect sizes, a measure of prior attainment and at least one comparison group was established for each class (typically either an equivalent class taught in the previous year by the same teacher, or a parallel class taught by another teacher). The mean effect size was 0.32

Inclusive versus Exclusive EM Processes in Relativistic Nuclear Systems

Connections are explored between exclusive and inclusive electron scattering within the framework of the relativistic plane-wave impulse approximation, beginning with an analysis of the model-independent kinematical constraints to be found in the missing energy--missing momentum plane. From the interplay between these constraints and the spectral function basic features of the exclusive and inclusive nuclear responses are seen to arise. In particular, the responses of the relativistic Fermi gas and of a specific hybrid model with confined nucleons in the initial state are compared in this work. As expected, the exclusive responses are significantly different in the two models, whereas the inclusive ones are rather similar. By extending previous work on the relativistic Fermi gas, a reduced response is introduced for the hybrid model such that it fulfills the Coulomb and the higher-power energy-weighted sum rules. While incorporating specific classes of off-shellness for the struck nucleons, it is found that the reducing factor required is largely model-independent and, as such, yields a reduced response that is useful for extracting the Coulomb sum rule from experimental data. Finally, guided by the difference between the energy-weighted sum rules of the two models, a version of the relativistic Fermi gas is devised which has the 0$^{\rm th}$, 1$^{\rm st}$ and 2$^{\rm nd}$ moments of the charge response which agree rather well with those of the hybrid model: this version thus incorporates {\em a priori} the binding and confinement effects of the stuck nucleons while retaining the simplicity of the original Fermi gas.Comment: LaTex file with 15 .ps figure

Measuring longitudinal amplitudes for electroproduction of pseudoscalar mesons using recoil polarization in parallel kinematics

We propose a new method for measuring longitudinal amplitudes for electroproduction of pseudoscalar mesons that exploits a symmetry relation for polarization observables in parallel kinematics. This polarization technique does not require variation of electron scattering kinematics and avoids the major sources of systematic errors in Rosenbluth separation.Comment: intended for Phys. Rev. C as a Brief Repor

Recognizing graphs close to bipartite graphs.

We continue research into a well-studied family of problems that ask if the vertices of a graph can be partitioned into sets A and B, where A is an independent set and B induces a graph from some specified graph class G. We let G be the class of k-degenerate graphs. The problem is known to be polynomial-time solvable if k=0 (bipartite graphs) and NP-complete if k=1 (near-bipartite graphs) even for graphs of diameter 4, as shown by Yang and Yuan, who also proved polynomial-time solvability for graphs of diameter 2. We show that recognizing near-bipartite graphs of diameter 3 is NP-complete resolving their open problem. To answer another open problem, we consider graphs of maximum degree D on n vertices. We show how to find A and B in O(n) time for k=1 and D=3, and in O(n^2) time for k >= 2 and D >= 4. These results also provide an algorithmic version of a result of Catlin [JCTB, 1979] and enable us to complete the complexity classification of another problem: finding a path in the vertex colouring reconfiguration graph between two given k-colourings of a graph of bounded maximum degree

Mass ratio from Doppler beaming and R{\o}mer delay versus ellipsoidal modulation in the Kepler data of KOI-74

We present a light curve analysis and radial velocity study of KOI-74, an eclipsing A star + white dwarf binary with a 5.2 day orbit. Aside from new spectroscopy covering the orbit of the system, we used 212 days of publicly available Kepler observations and present the first complete light curve fitting to these data, modelling the eclipses and transits, ellipsoidal modulation, reflection, and Doppler beaming. Markov Chain Monte Carlo simulations are used to determine the system parameters and uncertainty estimates. Our results are in agreement with earlier studies, except that we find an inclination of 87.0 \pm 0.4\degree, which is significantly lower than the previously published value. We find that the mass ratio derived from the radial velocity amplitude (q=0.104 \pm 0.004) disagrees with that derived from the ellipsoidal modulation (q=0.052 \pm 0.004} assuming corotation). This was found before, but with our smaller inclination, the discrepancy is even larger than previously reported. Accounting for the rapid rotation of the A-star is found to increase the discrepancy even further by lowering the mass ratio to q=0.047 \pm 0.004. These results indicate that one has to be extremely careful in using the amplitude of an ellipsoidal modulation signal in a close binary to determine the mass ratio, when a proof of corotation is not firmly established. The radial velocities that can be inferred from the detected Doppler beaming in the light curve are found to be in agreement with our spectroscopic radial velocity determination. We also report the first measurement of R{\o}mer delay in a light curve of a compact binary. This delay amounts to -56 \pm 17 s and is consistent with the mass ratio derived from the radial velocity amplitude. The firm establishment of this mass ratio at q=0.104 \pm 0.004 leaves little doubt that the companion of KOI-74 is a low mass white dwarf.Comment: 9 pages, 7 figures, 2 tables; accepted for publication in MNRA