Exposure to NO<inf>2</inf> in occupationalbuilt environmnets in urban centre in Lahore

Increased economic growth, urbanisation and substantial rise in automobile vehicles has contributed towards the elevated levels of air pollution in major cities in Pakistan. Aone week study was conducted by using passive samplers to assess NO2 concentration in occupational built environments at two most congested and populated sites of Lahore. Both sites were locatedon the busy roads of Lahore. At Site-I the highest concentration was in outdoors followed by corridor and indoor. While at Site II all the sampling location wereindoors and level were comparable to that of outdoor levelsat Site I. The results suggest the likely contribution of ambient sources in exposure to indoor NO2 in educational and other occupational built environments in urban centres

Small bowel perforation by a piece of china with a synchronous asymptomatic sigmoid carcinoma: A case report

A 75 year old gentleman who presented with an incarcerated paraumibilical hernia was found intraoperatively to have small bowel perforation due to a piece of china with a synchronous asymptomatic sigmoid carcinoma

Carbon nanotubes assisted analytical detection – sensing/delivery cues for environmental and biomedical monitoring

The architecture of carbon nanotubes (CNTs) demonstrate phenomenal electronic, mechanical, biological and thermal attributes for highly requisite real-time applications. For instance, electronic and biological features of CNTs are surprisingly striking to engineer robust sensing and/or delivery cues for environmental, analytical diagnostics, and biomedical settings. With CNTs enforcement, several types of pristine and hybrid nanomaterials have been fabricated, though using different support carriers and synthetic or biological materials and used as sensory items or exploited as drug delivery systems (DDSs). Regardless of intensive research and applied potentialities of CNTs, several concerns, such as biodegradability, biotoxicity, and biosafety remains challenging and should be dealt with care prior to design and fabrication. This is mainly because of the lacking standardized protocols and ramification of pristine CNTs or CNTs-based hybrid nano-constructs on the ecosystem and human body are not well-established. For the futuristic use of these remarkable materials in the environmental, analytical diagnostics, and biomedical settings, their biological attributes and multifunctional characteristics must be elucidated with state-of-the-art. Herein, we reviewed CNTs-assisted analytical detection potentialities at large, and sensing/delivery potentialities of CNTs-based cues, in particular for environmental and biomedical monitoring. Several examples are given with particular emphasis to biosensors, DDSs, and implantations of CNTs-based cues to recognize viruses, cancerous cells, glucose, DNA, volatile organic compounds (VOCs) and various inorganic gases. The review is wrapped-up with concluding notes and brief outlook over the futuristic developments to further insight the CNTs-based robust cues and their perspectives for commercialization

Rotating black rings on Taub-NUT

In this paper, we construct new solutions describing rotating black rings on Taub-NUT using the inverse-scattering method. These are five-dimensional vacuum space-times, generalising the Emparan-Reall and extremal Pomeransky-Sen'kov black rings to a Taub-NUT background space. When reduced to four dimensions in Kaluza-Klein theory, these solutions describe (possibly rotating) electrically charged black holes in superposition with a finitely separated magnetic monopole. Various properties of these solutions are studied, from both a five- and four-dimensional perspective.Comment: 33 pages, 3 figures, LaTe

Balanced electric-magnetic dihole in Kaluza-Klein theory

We present a four-dimensional double-black-hole (or dihole) solution in Kaluza-Klein theory, describing a superposition of an electrically charged and a magnetically charged black hole. This system can be balanced for appropriately chosen parameters, and the resulting space-time is completely regular on and outside the event horizons. This solution was constructed using the inverse-scattering method in five-dimensional vacuum gravity, in which it describes a rotating black ring surrounding a static black hole on a Taub-NUT background space. Various properties of this solution are studied, from both a four- and five-dimensional perspective.Comment: 33 pages, 6 figures; v2: expanded discussion of phase space, published versio

Supergravity Solutions from Floating Branes

We solve the equations of motion of five-dimensional ungauged supergravity coupled to three U(1) gauge fields using a floating-brane Ansatz in which the electric potentials are directly related to the gravitational warp factors. We find a new class of non-BPS solutions, that can be obtained linearly starting from an Euclidean four-dimensional Einstein-Maxwell base. This class - the largest known so far - reduces to the BPS and almost-BPS solutions in certain limits. We solve the equations explicitly when the base space is given by the Israel-Wilson metric, and obtain solutions describing non-BPS D6 and anti-D6 branes kept in equilibrium by flux. We also examine the action of spectral flow on solutions with an Israel-Wilson base and show that it relates these solutions to almost-BPS solutions with a Gibbons-Hawking base.Comment: 24 pages, 1 figur

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

Current antibiotics tend to be broad spectrum, leading to indiscriminate killing of commensal bacteria and accelerated evolution of drug resistance. Here, we use CRISPR-Cas technology to create antimicrobials whose spectrum of activity is chosen by design. RNA-guided nucleases (RGNs) targeting specific DNA sequences are delivered efficiently to microbial populations using bacteriophage or bacteria carrying plasmids transmissible by conjugation. The DNA targets of RGNs can be undesirable genes or polymorphisms, including antibiotic resistance and virulence determinants in carbapenem-resistant Enterobacteriaceae and enterohemorrhagic Escherichia coli. Delivery of RGNs significantly improves survival in a Galleria mellonella infection model. We also show that RGNs enable modulation of complex bacterial populations by selective knockdown of targeted strains based on genetic signatures. RGNs constitute a class of highly discriminatory, customizable antimicrobials that enact selective pressure at the DNA level to reduce the prevalence of undesired genes, minimize off-target effects and enable programmable remodeling of microbiota.National Institutes of Health (U.S.) (New Innovator Award 1DP2OD008435)National Centers for Systems Biology (U.S.) (Grant 1P50GM098792)United States. Defense Threat Reduction Agency (HDTRA1-14-1-0007)Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (W911NF13D0001)National Institute of General Medical Sciences (U.S.) (Interdepartmental Biotechnology Training Program 5T32 GM008334)Fonds de la recherche en sante du Quebec (Master's Training Award

Stationary Black Holes: Uniqueness and Beyond

The spectrum of known black-hole solutions to the stationary Einstein equations has been steadily increasing, sometimes in unexpected ways. In particular, it has turned out that not all black-hole-equilibrium configurations are characterized by their mass, angular momentum and global charges. Moreover, the high degree of symmetry displayed by vacuum and electro-vacuum black-hole spacetimes ceases to exist in self-gravitating non-linear field theories. This text aims to review some developments in the subject and to discuss them in light of the uniqueness theorem for the Einstein-Maxwell system.Comment: Major update of the original version by Markus Heusler from 1998. Piotr T. Chru\'sciel and Jo\~ao Lopes Costa succeeded to this review's authorship. Significantly restructured and updated all sections; changes are too numerous to be usefully described here. The number of references increased from 186 to 32

Blechnum Orientale Linn - a fern with potential as antioxidant, anticancer and antibacterial agent

<p>Abstract</p> <p>Background</p> <p><it>Blechnum orientale </it>Linn. (<it>Blechnaceae</it>) is used ethnomedicinally for the treatment of various skin diseases, stomach pain, urinary bladder complaints and sterilization of women. The aim of the study was to evaluate antioxidant, anticancer and antibacterial activity of five solvent fractions obtained from the methanol extract of the leaves of <it>Blechnum orientale </it>Linn.</p> <p>Methods</p> <p>Five solvent fractions were obtained from the methanol extract of <it>B. orientale</it> through successive partitioning with petroleum ether, chloroform, ethyl acetate, butanol and water. Total phenolic content was assessed using Folin-Ciocalteu's method. The antioxidant activity was determined by measuring the scavenging activity of DPPH radicals. Cytotoxic activity was tested against four cancer cell lines and a non-malignant cell using MTT assay. Antibacterial activity was assessed using the disc diffusion and broth microdilution assays. Standard phytochemical screening tests for saponins, tannins, terpenoids, flavonoids and alkaloids were also conducted.</p> <p>Results</p> <p>The ethyl acetate, butanol and water fractions possessed strong radical scavenging activity (IC₅₀8.6-13.0 μg/ml) and cytotoxic activity towards human colon cancer cell HT-29 (IC₅₀27.5-42.8 μg/ml). The three extracts were also effective against all Gram-positive bacteria tested: <it>Bacillus cereus, Micrococcus luteus</it>, methicillin-susceptible <it>Staphylococcus aureus </it>(MSSA), methicillin-resistant <it>Staphylococcus aureus </it>(MRSA) and <it>Stapylococcus epidermidis</it>(minimum inhibitory concentration MIC 15.6-250 μg/ml; minimum bactericidal concentration MBC 15.6-250 μg/ml). Phytochemical analysis revealed the presence of flavonoids, terpenoids and tannins. Ethyl acetate and butanol fractions showed highest total phenolic content (675-804 mg gallic acid equivalent/g).</p> <p>Conclusions</p> <p>The results indicate that this fern is a potential candidate to be used as an antioxidant agent, for colon cancer therapy and for treatment of MRSA infections and other MSSA/Gram-positive bacterial infectious diseases.</p