A Kinesin Driven Enzyme Linked Immunosorbant Assay (ELISA) for Ultra Low Protein Detection Applications

<p>Gene Ontology bar charts for three different criteria: biological process (A), cell localization (B) and molecular function (C). The list of differentially expressed genes after RSV diet in neocortex were classified by Gene Ontology (GO) and drawn using Panther (<a href="https://www.pantherdb.org/" target="_blank">www.pantherdb.org/</a>)</p

Network analysis using STRING.

<p>The list of differentially expressed genes in neocortex after RSV diet was subjected to Network analysis using the STRING software as described in Methods. Square interaction score was set at medium confidence (0.4; scores range from highest, 0.9, to low, 0.15, confidence). Other selected parameters were: average node degree: 1.12, local clustering coefficient: 0.32 and active interaction sources including: text mining, experiments, databases, co-expression, neighborhood, gene fusion and co-occurrence. Moreover, no restrictions were forced in the number of interactions to show. Overexpressed genes are shown within red rectangles. The colors of the edges represent the different types of protein associations, either from known or predicted interactions: from curated databases (blue), experimentally determined (magenta), text mining (green) and co-expression (black).</p

Venn diagram constructed with lists of differentially expressed genes corresponding to the three tissues (neocortex, heart and muscle) as identified using the GEO2R tool.

<p>The lists of genes were first curated as indicated in Material and Methods.</p

Gene expression evaluated by reverse transcription, quantitative real-time PCR (RT-qPCR) in control and in 100 μM RSV-treated C6 glioma cells.

<p>mRNA samples isolated from cells incubated for 24 h were analyzed by RT-qPCR using specific probes for <i>Atp1b2</i>, <i>Vamp2</i>, <i>Rab2a</i>, and <i>Dnm1</i>. β-actin mRNA was used as control. Data are mean ± SEM of four independent experiments. * p < 0.05 according to Student’s t test.</p

Selected differentially expressed genes.

<p>Selected differentially expressed genes.</p

Number of differentially expressed genes detected by GeneSpring depending on p-value and absolute fold change (FC) values.

<p>Analysis of data, from control- and RSV-enriched diet both in quintuplicates, were performed using moderated T-test, the multiple testing correction of Benjamini-Hochberg and asymptotic p-value (adjusted) computation.</p

Correlation between serum TIMP-1 levels and other baseline clinical and biochemical characteristics.

<p>TIMP =  tissue inhibitor of matrix metalloproteinase; GCS =  Glasgow Coma Scale; TNF  =  tumor necrosis factor; PAI  =  plasminogen activator inhibitor; INR  =  international normalized ratio; aPTT  =  activated partial thromboplastin time; MMP  =  matrix metalloproteinase. Bonferroni correction to control the multiple testing problem (0.05/11 = 0.004) was used. Only <i>P</i>-values lower than 0.004 were considered statistically significant.</p

Multiple binomial logistic regression analysis of variables to predict 30-day mortality.

<p>TIMP  =  tissue inhibitor of matrix metalloproteinase; APACHE II  =  Acute Physiology and Chronic Health Evaluation; GCS Glasgow Coma Scale</p

Baseline clinical and biochemical characteristics of survivor and non-survivor patients.

<p>P 25–75 = percentile 25^th–75^th; PaO₂ = pressure of arterial oxygen/fraction inspired oxygen; FIO₂  =  pressure of arterial oxygen/fraction inspired oxygen; ISS  =  Injury Severity Score; INR  =  international normalized ratio; aPTT  =  activated partial thromboplastin time; APACHE II  =  Acute Physiology and Chronic Health Evaluation; ICP  =  intracranial pressure; CPP  =  cerebral perfusion pressure; TIMP  =  tissue inhibitor of matrix metalloproteinase; MMP  =  matrix metalloproteinase; TNF  =  tumor necrosis factor; PAI  =  plasminogen activator inhibitor</p

Receiver operation characteristic analysis using TIMP-1 serum levels as predictor of mortality at 30 days.

<p>Receiver operation characteristic analysis using TIMP-1 serum levels as predictor of mortality at 30 days.</p