6 research outputs found
Hematological results from primary blood samples (n = 10) that were used to derive blood preparations.
<p>Hematological results from primary blood samples (n = 10) that were used to derive blood preparations.</p
Coefficients of multivariate regression models for mtDNAcn(WB) measured in blood preparations (n = 46).
<p>Coefficients of multivariate regression models for mtDNAcn(WB) measured in blood preparations (n = 46).</p
Hematological results from primary blood samples (n = 10) that were used to derive blood preparations.
<p>Hematological results from primary blood samples (n = 10) that were used to derive blood preparations.</p
Scatter plot of the change of mtDNAcn(WB) per unit increase of the platelets/leukocyte ratio.
<p>This scatter plot shows the change of mtDNAcn(WB) per unit increase of the platelets/leukocyte ratio. Data from 46 preparations are shown. Individual mtDNAcn(WB) for each preparation are plotted as white circles. The line represents the fit of a linear model (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163770#pone.0163770.t003" target="_blank">Table 3</a>, Model 6). mtDNAcn(WB): mitochondrial DNA copy number measured in whole blood.</p
Platelet count, Leukocyte count and mtDNAcn(WB) distributions in all subjects from the AWHS cohort study (n = 3389).
<p>Platelet count, Leukocyte count and mtDNAcn(WB) distributions in all subjects from the AWHS cohort study (n = 3389).</p
Local Temperature Increments and Induced Cell Death in Intracellular Magnetic Hyperthermia
The generation of
temperature gradients on nanoparticles heated
externally by a magnetic field is crucially important in magnetic
hyperthermia therapy. But the intrinsic low heating power of magnetic
nanoparticles, at the conditions allowed for human use, is a limitation
that restricts the general implementation of the technique. A promising
alternative is local intracellular hyperthermia, whereby cell death
(by apoptosis, necroptosis, or other mechanisms) is attained by small
amounts of heat generated at thermosensitive intracellular sites.
However, the few experiments conducted on the temperature determination
of magnetic nanoparticles have found temperature increments that are
much higher than the theoretical predictions, thus supporting the
local hyperthermia hypothesis. Reliable intracellular temperature
measurements are needed to get an accurate picture and resolve the
discrepancy. In this paper, we report the real-time variation of the
local temperature on γ-Fe2O3 magnetic
nanoheaters using a Sm3+/Eu3+ ratiometric luminescent
thermometer located on its surface during exposure to an external
alternating magnetic field. We measure maximum temperature increments
of 8 °C on the surface of the nanoheaters without any appreciable
temperature increase on the cell membrane. Even with magnetic fields
whose frequency and intensity are still well within health safety
limits, these local temperature increments are sufficient to produce
a small but noticeable cell death, which is enhanced considerably
as the magnetic field intensity is increased to the maximum level
tolerated for human use, consequently demonstrating the feasibility
of local hyperthermia