A new analytical method for determination of ledipasvir and sofosbuvir in pharmaceutical formulations by HPLC method

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Ledipasvir in Tablet dosage form. Chromatogram was run through Std Discovery C8 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Sofosbuvir and Ledipasvir were found to be 2.367 min and 3.436 min. %RSD of the Sofosbuvir and Ledipasvir were and found to be 0.6 and 0.5 respectively. %Recovery was obtained as 99.61% and 99.80% for Sofosbuvir and Ledipasvir respectively. LOD, LOQ values obtained from regression equations of Sofosbuvir and Ledipasvir were 0.67, 2.02 and 0.70, 2.12 respectively. Regression equation of Sofosbuvir is y = 4266.x + 7700, and y = 4861.x + 2656.of Ledipasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries

Ultralow-Power and Secure S-Box Circuit Using FinFET Based ECRL Adiabatic Logic

Advanced Encryption Standard (AES) is the widely used technique in critical cyber security applications. In AES architecture S-box is the most important block. However, the power consumed by      S-box is 75% of the total AES design. The   S-box is also prone to Differential Power Analysis (DPA) attack which is one of the most threatening types of attacks in cryptographic systems. In this paper, a     three-stage positive polarity Reed-Muller (PPRM) S-box is implemented with 45nm FinFET using Efficient Charge Recovery Logic (ECRL) to reduce power consumption. The simulation results indicate up to 66% power savings for FinFET based S-box as compared to CMOS design. Further, the FinFET ECRL 8-bit     S-box circuit is evaluated for transitional energy fluctuations and peak current traces to compare its resistance against side-channel attacks. The lower energy variations and uniform current trace exhibit the improved security performance of the circuit to withstand DPA and Differential Electromagnetic Radiation Attacks (DEMA)

Studies on the extraction and characterization of thermostable a-amylase from pericarp of Borassus indica

Thermostable a-amylase was extracted and characterized from the fruits (pericarp) of Borassus indica. Analysis on the influence of various physico-chemical parameters on the extracted enzyme revealed a Vmax of 0.793 and a Km of 0.022. The optimum temperature was found to be 370C at pH 4.5. The stability studies on enzyme activity envisaged that the enzyme is stable up to 800C and retained its activity over a wide range of pH (4.0 – 8.5). Significant enhancement in the enzyme activity was observed in the presence of metal ions like Manganese and Strontium and an insignificant decrement in the presence of Sodium ions.African Journal of Biotechnology Vol. 4 (3), pp. 289-291, 200

Influence of Exogenous Glycinebetaine on Hot Pepper under Water Stress

A study was conducted to evaluate the effect of exogenous application of glycinebetaine (GB) on physiological response in hot-pepper (Capsicum annuum L. vs. Arka Lohit and Pusa Jwala) under water stress. Glycinebetaine was applied to seeds as well as plants through foliar applications. Water stress affected considerably the morphophysiological parameters in both the cultivars. However, in glycinebetaine (GB) treated plants, plant height, leaf area (LA), flower and fruit number and total dry matter (TDM) were greater compared to the untreated stress plants (T4) under water stress. Glycinebetaine application enhanced the photosynthesis (PN) in water deficit experiencing plants, mostly due to a greater stomatal conductance (gs) and carboxylation efficiency of CO2 assimilation. In both the cultivars after 12 day of stress, the PN decreased from 10.1 to 1.0-1.3 μ mol m-2 s-1 in untreated stressed plants (T4), while in the treated stressed plants PNhad reduced to 2.0 - 3.0 μ mol m-2 s-1 (T1 - T3). The application of GB increased the WUE in both the cultivars. The better WUE in treated plants of hot-pepper under stress was attributed to the improved PN. The higher per plant yield in the GB applied plants under stress in both the cultivars associated with higher PNrate, gs and WUE in treated plants. Though there was an increase in PN rate, WUE and plant yield in the treated plants (T1 - T3), the better results were found in the plants (T2) where seeds were treated and foliar application was given at the time of imposing stress

HIV/AIDS-tuberculosis (pulmonary and extra pulmonary) co-infection: CD4 correlation

Background: AIDS is the leading cause of death among people 15-59 years old in low income countries. Worldwide, approximately one-third of all AIDS-related deaths are associated with TB. TB is the primary cause of death for 10-15% of patients with HIV infection. So the present study was conducted to find the correlation between sputum positivity and CD4 cell count in patients with HIV/AIDS-Tuberculosis co-infection.Methods: The present study was a retrospective hospital based study of patients with HIV/AIDS-Tuberculosis co-infection, attending ART centre, department of medicine, Osmania general hospital, Hyderabad, Telangana, India between November 2014 to September 2015. Data included clinical profile, complete blood picture, renal and liver function tests, sputum microscopy and C/S and chest X-Ray and others as and when required.Results: We included 180 HIV/AIDS infected patients on ART with tuberculosis (TB) co-infection. Out of 180 patients, 132 were males and 48 were females. Among male’s ≥40 (51.51%) year’s age group and among females 30-39 (56.25%) year’s age group was the most commonly affected. Out of 180 cases 60 were sputum positive. 60 sputum negative pulmonary tuberculosis and 60 were extra pulmonary TB. CD4 cell count was <200/mm3 in 36 (60%) of sputum positive TB, 43 (71.7%) of sputum negative pulmonary TB & 39 (65%) of extra pulmonary TB patients. CD4 cell count was 200-400/mm3 in 16(26.7%) of sputum positive pulmonary TB, 13 (21.7%) of sputum negative TB and 19 (31.7%) of extra pulmonary TB patients. CD4 cell count was >400/mm3 in 8 (13.3%) of sputum positive pulmonary TB, 4 (6.6%) of sputum negative pulmonary TB and 2 (3.3%) of extra pulmonary TB patients.Conclusions: Present study concludes that male sex and age group >30 years were the commonly affected population. All forms of tuberculosis were common when CD4 count was <200 cells/mm3. The sputum negativity was higher with lower CD4 counts.

Farming of cobia, Rachycentron canadum (Linnaeus 1766) in open sea floating cages in India

Experiments on culture and growth performance of cobia were undertaken in marine cages installed in the Arabian Sea off Karwar on the south-west coast of India. Hatchery produced cobia fingerlings were stocked at two densities of 3.5 fish cubic m-1 and 14.1 fish cubic m-1 in circular HDPE cages. Mean weight and specific growth rate (SGR) of cobia stocked @ 3.5 fish cubic m -1 were 10.5 kg and 2.2% day-1, respectively, at 300 days of culture (DOC). Cobia stocked @ of 14.1 fish cubic m-1 attained an average weight of 3.68 kg and SGR of 1.9% day-1 at 300 DOC. SGR was found to be positively correlated with water temperature and salinity during the culture period. No significant variation was found in SGR and feed conversion ratio of cobia stocked at two different densities. However, absolute growth rate, relative growth rate and mean weight of cobia varied significantly between the two groups

Evaluation of Effectiveness of Wavelet Based Denoising Schemes Using ANN and SVM for Bearing Condition Classification

The wavelet based denoising has proven its ability to denoise the bearing vibration signals by improving the signal-to-noise ratio (SNR) and reducing the root-mean-square error (RMSE). In this paper seven wavelet based denoising schemes have been evaluated based on the performance of the Artificial Neural Network (ANN) and the Support Vector Machine (SVM), for the bearing condition classification. The work consists of two parts, the first part in which a synthetic signal simulating the defective bearing vibration signal with Gaussian noise was subjected to these denoising schemes. The best scheme based on the SNR and the RMSE was identified. In the second part, the vibration signals collected from a customized Rolling Element Bearing (REB) test rig for four bearing conditions were subjected to these denoising schemes. Several time and frequency domain features were extracted from the denoised signals, out of which a few sensitive features were selected using the Fisher’s Criterion (FC). Extracted features were used to train and test the ANN and the SVM. The best denoising scheme identified, based on the classification performances of the ANN and the SVM, was found to be the same as the one obtained using the synthetic signal

Broodstock development and controlled breeding of cobia Rachycentron canadum (Linnaeus 1766) from Indian seas

Cobia, Rachycentron canadum has emerged as one of the topmost finfish species for mariculture. In India, cobia broodstock was developed and induced breeding was achieved for the first time at Mandapam Regional Centre of the Central Marine Fisheries Research Institute (CMFRI). The broodstock was developed in sea cages of 6 m diameter and 3.5 m depth. Sexes were separated about two months prior to the onset of breeding season and stocked in separate cages. During March 2010, a female with intra-ovarian egg diameter of 700 μ along with two males were selected for induced spawning. The brooders were induced with human chorionic gonadotropin (hCG) at doses of 500 IU per kg body weight for female and 250 IU per kg body weight for males. Spawning was noted after 39 h of intra-muscular injection. The total eggs spawned were estimated as 2.1 million. About 90% fertilization was recorded (fertilized eggs amounted to 1.9 million). The eggs were collected using a 500 μ mesh net and stocked in incubation tanks at varying densities. The eggs hatched after 22 h of incubation at a temperature range of 28-30 ºC. The percentage of hatching was 80% and the total number of newly hatched larvae was estimated as 1.5 millio

Marine cage farming of Asian seabass under participatory mode - A success story

Karwar Research Centre of ICAR-CMFRI in association with the Directorate of fisheries, Govt. of Goa identified Polem village ( 14° 54' 21.12" N ; 74° 04' 32.20" E) in south Goa for the cage culture demonstration under the proejct 'National Innovations on Climate Resilient Agriculture (NICRA)'. Awareness programmes were conducted for the fishermen communities in the village regarding the present status of marine fisheries resources of India and future prospects