COMPARATIVE STUDY OF EFFICACY OF JATYADI GHRITA PICHU AND YASTHIMADHU GHRITA PICHU IN THE MANAGEMENT OF PARIKARTIKA (FISSURE- IN-ANO)

The condition Parikartika has been mentioned in the Ayurvedic literature as one of the fifteen kinds of disorders which may result from an injudicious use of purgatives owing to the ignorance of the physician or of the patient. The acute fissure is a superficial splitting of the anoderm characterized by severe pain, sometimes associated with bleeding per rectum during/after defaecation.Application of local anesthetics, anal dilatation, sphincterotomy, and fissurectomy (chronic fissure) are usually in practice. But these procedures have sometimes associated with some complications like post operative anal stenosis, sphincter incontinence etc. To overcome such problems and to provide cheap, simple, ambulatory and effective treatment, a combined therapy has been kept on trial on the basis of the treatment mentioned in the ancient literature in the management. Aim and objectives: Evaluation of clinical efficacy of Pichu application of Jatyadi Grihta and Yasthimadhu Grihta in the management of Fissure- in-ano (Parikartika). Materials & Methods: Taking this view in to consideration, a modified regimen with Jatyadi ghtrita and Yasthimadhu ghrita in Pichu form in two different groups along with Panchasakara churna have been kept on trial on 100 patients in each group in the Ano-Rectal Clinic of the Institute. Results and Conclusion: The results were assessed and compared and it was found that both the medicines were equally effective without any complications in the management of Fissure-in-ano

[3+2] Cycloadditions. Part XXXIII. Selective cycloadditions of C-(1-naphthyl)-N-methyl nitrone and C-phenyl-N-benzyl nitrone to α,β-unsaturated carbonyl compounds1,2

488-501[3+2] Cycloadditions [32CA] involving nitrones as 1,3-dipolar species to alkenes yield isoxazolidines, which on further transformations can be converted to naturally occurring bioactive compounds. Thus the 32CA route provides conversion of simple natural products to more complex natural occurring bioactive nitrogen heterocycles, and close analogues. The present work deals with 32CA between the nitrones C-(1-naphthyl)-N-methyl nitrone and C-phenyl-N-benzyl nitrone to α,β-unsaturated carbonyl compounds as dipolarophiles, viz. arylidene malonate esters, methyl cinnamate and benzylidene acetophenone (chalcone). Methyl cinnamate is a naturally occurring compound, while the chalcone scaffold is present in several natural products. Structure elucidation of the generated cycloadducts have been achieved by means of detailed spectroscopic and XRD studies. All the cycloadditions investigated occurr regioselectively to yield tetra/penta-substituted isoxazolidines, where the carbonyl group(s) are situated at the 4-position of the isoxazolidine ring. DFT computations including optimised geometries, FMO energies, electronic chemical potentials, chemical hardness, chemical softness and reactivity indices of a number of the reactants have been calculated at DFT/B3LYP/6-31++G(d,p) level of theory. The calculated reactivity indices have been used to analyse the 32CAs studied and to predict the regioselectivities; the predictions are in excellent accord with the experimental results

QUALITY CHARACTERIZATION OF MADHUYASHTI GHRITA

Madhuyashti Ghrita, is an Ayurvedic medicated ghee preparation containing Yashtimadhu. It is used for treating external ulcers and wounds in vital points of the body. However, there is no quality monograph available for Madhuyashti Ghrita. Hence, the present work was carried out to characterize this Ghrita to confirm its identity, quality and purity. This work reported various pharmacognostic and physicochemical parameters of Madhuyashti Ghrita along with its TLC based rapid fingerprinting as per the present standards of Ayurvedic Pharmacopoeia of India. Moreover, the presence of toxic contaminants like heavy metals, and microbial load were also evaluated. In organoleptic evaluation, the Ghrita was found to be semi–solid and dark yellow in colour, sweet and bitter in taste with a characteristic and pleasant odour. The values of the physicochemical parameters such as acid value, saponification value, peroxide value, refractive index, rancidity and pH value of the Ghrita were also assed. In the microscopical analysis of the rhizomes of Yashtimadhu, the presence of pitted vessels, fibres, prismatic crystals and starch grains were noted as a confirmation characteristic for the genuinely of this raw material in Madhuyashti Ghrita. The amount of heavy metals such arsenic, lead, mercury, cadmium, nickel, zinc, copper and chromium as well as total bacterial count and total fungal count were found to be much below their API limits. The TLC showed different characteristic spots indicating the presence of Yashtimadhu in this product. The parameters of Madhuyashti Ghrita presented in this paper may be utilized for preparing a quality monograph for this product

Improvised storage of Cassia fistula L. fruit pod with special references to Ayurvedic principles and practices by traditional text: An analytical investigation

317-322Cassia fistula L. (Sanskrit: Aragvadha, family: Caesalpinaceae) is used as a mild laxative in traditional medicine. Ancient texts advocate specific storage of its matured and ripe fruits under a pit filled with sand or soil. The present study was designed to compare the physicochemical, organoleptic and other biochemical parameters of the fruit pulp, stored under usual and specific conditions as mentioned in ancient texts. The sample kept under a pit showed higher total phenolics, flavonoids and anthraquinone levels along with reduced total and reducing sugars. The increased antioxidant activity of the pit-stored sample due to higher total phenolics and flavonoids levels as revealed from the DPPH radical scavenging assay may enhance its medicinal attributes, justifying ancient claim of specific storage of the fruits

Seasonal dynamics of Shatavarin-IV, a potential biomarker of Asparagus racemosus by HPTLC: Possible validation of the ancient Ayurvedic text.

The medicinal property of Asparagus racemosus is primarily attributed to its constituent steroidal saponins, particularly the major component, shatavarin-IV. Thus, it can serve as a biomarker and its level can decide of the utility of the plant cultivar as a drug. Hence, a sensitive, reliable and quantitative High Performance Thin Layer Chromatography (HPTLC) method has been established for quantification of shatavarin-IV in the methanolic extracts of the roots collected in both summer and rainy seasons. The extracts of the powders of dried roots were applied to silica gel 60 F254 aluminum-supported precoated TLC plates and developed with n-hexane: ethyl acetate: methanol, 80:10:10 (v/v), as the mobile phase. Shatavarin-IV was detected and quantified by densitometry at λ = 336 nm. The accuracy of the method was checked by conducting recovery studies at three different levels of shatavarin-IV. The average recovery was found to be 101% and 107% for summer and rainy seasons respectively. The shatavarin-IV contents, as estimated by the proposed method were 12.5 μg gm-1 and 10.9 μg gm-1 in summer and rainy roots respectively. The entire method was performed six times (n=6) to check the repeatability. The proposed HPTLC method for quantitative monitoring of shatavarin-IV in A. racemosus roots collected in different seasons strictly adhered to the validation issues laid down by the ICH guidelines. The method is reliable reproducible and highly precise and selective

Seasonal dynamics of Shatavarin-IV, a potential biomarker of Asparagus racemosus by HPTLC: Possible validation of the ancient Ayurvedic text.

174-181The medicinal property of Asparagus racemosus is primarily attributed to its constituent steroidal saponins, particularly the major component, shatavarin-IV. Thus, it can serve as a biomarker and its level can decide of the utility of the plant cultivar as a drug. Hence, a sensitive, reliable and quantitative High Performance Thin Layer Chromatography (HPTLC) method has been established for quantification of shatavarin-IV in the methanolic extracts of the roots collected in both summer and rainy seasons. The extracts of the powders of dried roots were applied to silica gel 60 F254 aluminum-supported precoated TLC plates and developed with n-hexane: ethyl acetate: methanol, 80:10:10 (v/v), as the mobile phase. Shatavarin-IV was detected and quantified by densitometry at λ = 336 nm. The accuracy of the method was checked by conducting recovery studies at three different levels of shatavarin-IV. The average recovery was found to be 101% and 107% for summer and rainy seasons respectively. The shatavarin-IV contents, as estimated by the proposed method were 12.5 μg gm-1 and 10.9 μg gm-1 in summer and rainy roots respectively. The entire method was performed six times (n=6) to check the repeatability. The proposed HPTLC method for quantitative monitoring of shatavarin-IV in A. racemosus roots collected in different seasons strictly adhered to the validation issues laid down by the ICH guidelines. The method is reliable reproducible and highly precise and selective