e-KrishiShiksha : e-Learning Portal on Agriculture Education in India

The aim of the study is to understand the e-KrishiShiksha, an e-Learning platform for agriculture education in India. An e-learning portal called e-KrishiShiksha had a significant impact on India\u27s agricultural research and extension programs. According to the study, out of seven-degree programs, most students (56.2%) registered for the B.Sc. (agricultural) degree program. This was followed by the B.Sc. (Horticulture) 15.3%, B.Tech. (Agricultural Engineering) 11.3%, and B.Tech. (Dairy Technology) 6.4%. According to the report, university-wise across the country majority of users registered in MPKV Rahuri University (8.9%), followed by IGKV Raipur (5.6%) and TNAU, Coimbatore (4.7%). Furthermore, it has been noted that the majority of undergraduate students across all seven disciplines have registered for e-KrishiShiksha

Stochastically Simulating the Effects of Requirements Creep on Software Development Risk Management

One of the major chronic problems in software development is the fact that application requirements are almost never stable and fixed. Creeping user requirements have been troublesome since the software industry began. Several empirical studies have reported that volatile requirements are a challenging factor in most information systems development projects. Software process simulation modeling has increasingly been used for a variety of issues during software development. The management of software development risks is one of them. This study presents an approach for simulating and analyzing the effect of Requirements Creep on certain software development risk management activities. The proposed algorithm is based on stochastic simulation and has been implemented using C

Sulfadiazine induced Stevens Johnson syndrome in toxoplasmosis patient: a case report

Stevens johnson syndrome is an acute, self-limited disease, presenting as severe mucosal erosions with widespread erythematous, cutaneous macules or atypical targets. Even though sulfadiazine has been mainly associated with haematological-related adverse effects, sulfadiazine induced skin necrosis has received less attention or went unrecognized. Here is a 29-year- old Indian male received T. Sulfadiazine 500mg 1-1-1-1 and experienced a severe skin reaction which was diagnosed as stevens johnson syndrome (SJS). The above drug will be implicated in cases of stevens johnson syndrome (SJS)/toxic epidermal necrosis (TEN). There are few case reports of that have been associated with stevens johnson syndrome (SJS)/toxic epidermal necrosis (TEN). We hope that this case report creates awareness to the health care professionals. Clinicians must be aware of these adverse reactions and advise their patients to contact them as soon as they observe any unexpected clinical response. Early diagnosis helps the clinician to elude secondary infection and subsequent complications. The offending drug should be discontinued and never be rechallenged.

IPAVS: Integrated Pathway Resources, Analysis and Visualization System

Integrated Pathway Resources, Analysis and Visualization System (iPAVS) is an integrated biological pathway database designed to support pathway discovery in the fields of proteomics, transcriptomics, metabolomics and systems biology. The key goal of IPAVS is to provide biologists access to expert-curated pathways from experimental data belonging to specific biological contexts related to cell types, tissues, organs and diseases. IPAVS currently integrates over 500 human pathways (consisting of 24 574 interactions) that include metabolic-, signaling- and disease-related pathways, drug–action pathways and several large process maps collated from other pathway resources. IPAVS web interface allows biologists to browse and search pathway resources and provides tools for data import, management, visualization and analysis to support the interpretation of biological data in light of cellular processes. Systems Biology Graphical Notations (SBGN) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway notations are used for the visual display of pathway information. The integrated datasets in IPAVS are made available in several standard data formats that can be downloaded. IPAVS is available at: http://ipavs.cidms.org

Sumoylation of the basic helix-loop-helix transcription factor Sharp-1 regulates recruitment of the histone methyltransferase G9a and function in myogenesis

10.1074/jbc.M113.463257Journal of Biological Chemistry2882417654-1766

Pro-Inflammatory Cytokines, IFNγ and TNFα, Influence Immune Properties of Human Bone Marrow and Wharton Jelly Mesenchymal Stem Cells Differentially

BACKGROUND: Wharton's jelly derived stem cells (WJMSCs) are gaining attention as a possible clinical alternative to bone marrow derived mesenchymal stem cells (BMMSCs) owing to better accessibility, higher expansion potential and low immunogenicity. Usage of allogenic mesenchymal stem cells (MSC) could be permissible in vivo only if they retain their immune properties in an inflammatory setting. Thus the focus of this study is to understand and compare the immune properties of BMMSCs and WJMSCs primed with key pro-inflammatory cytokines, Interferon-gamma (IFNgamma) and Tumor Necrosis Factor-alpha (TNFalpha). METHODOLOGY/PRINCIPAL FINDINGS: Initially the effect of priming on MSC mediated suppression of alloantigen and mitogen induced lymphoproliferation was evaluated in vitro. Treatment with IFNgamma or TNFalpha, did not ablate the immune-suppression caused by both the MSCs. Extent of immune-suppression was more with WJMSCs than BMMSCs in both the cases. Surprisingly, priming BMMSCs enhanced suppression of mitogen driven lymphoproliferation only; whereas IFNgamma primed WJMSCs were better suppressors of MLRs. Further, kinetic analysis of cytokine profiles in co-cultures of primed/unprimed MSCs and Phytohematoagglutinin (PHA) activated lymphocytes was evaluated. Results indicated a decrease in levels of pro-inflammatory cytokines. Interestingly, a change in kinetics and thresholds of Interleukin-2 (IL-2) secretion was observed only with BMMSCs. Analysis of activation markers on PHA-stimulated lymphocytes indicated different expression patterns in co-cultures of primed/unprimed WJMSCs and BMMSCs. Strikingly, co-culture with WJMSCs resulted in an early activation of a negative co-stimulatory molecule, CTLA4, which was not evident with BMMSCs. A screen for immune suppressive factors in primed/unprimed WJMSCs and BMMSCs indicated inherent differences in IFNgamma inducible Indoleamine 2, 3-dioxygenase (IDO) activity, Hepatocyte growth factor (HGF) and Prostaglandin E-2 (PGE2) levels which could possibly influence the mechanism of immune-modulation. CONCLUSION/SIGNIFICANCE: This study demonstrates that inflammation affects the immune properties of MSCs distinctly. Importantly different tissue derived MSCs could utilize unique mechanisms of immune-modulation

G9a, a multipotent regulator of gene expression

10.4161/epi.23331Epigenetics8116-2

G9a mediates Sharp-1–dependent inhibition of skeletal muscle differentiation

10.1091/mbc.E12-04-0311Molecular Biology of the Cell23244778-478

G9a mediates Sharp-1–dependent inhibition of skeletal muscle differentiation

10.1091/mbc.E12-04-0311Molecular Biology of the Cell23244778-478