3 research outputs found
Molecular imprinted nanoelectrodes for ultra sensitive detection of ovarian cancer marker
The relentless discovery of cancer biomarkers demands improved methods for their detection. In this
work, we developed protein imprinted polymer on three-dimensional gold nanoelectrode ensemble
(GNEE) to detect epithelial ovarian cancer antigen-125 (CA 125), a protein biomarker associated with
ovarian cancer. CA 125 is the standard tumor marker used to follow women during or after treatment for
epithelial ovarian cancer. The template protein CA 125 was initially incorporated into the thin-film coating
and, upon extraction of protein from the accessible surfaces on the thin film, imprints for CA 125 were
formed. The fabrication and analysis of the CA 125 imprinted GNEE was done by using cyclic voltammetry
(CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS)
techniques. The surfaces of the very thin, protein imprinted sites on GNEE are utilized for immunospecific
capture of CA 125 molecules, and the mass of bound on the electrode surface can be detected as a
reduction in the faradic current from the redox marker. Under optimal conditions, the developed sensor
showed good increments at the studied concentration range of 0.5–400 U mL−1. The lowest detection
limit was found to be 0.5 U mL−1. Spiked human blood serum and unknown real serum samples were
analyzed. The presence of non-specific proteins in the serum did not significantly affect the sensitivity
of our assay. Molecular imprinting using synthetic polymers and nanomaterials provides an alternative
approach to the trace detection of biomarker proteins