193 research outputs found

    An Analysis of Cooperating Teacher Feedback: A Qualitative Inquiry

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    Cooperating teachers are a key component to the success of student teaching internships, serving an integral part in “raising” a teacher. To effectively facilitate the student teaching internship, teacher preparation programs must identify cooperating teachers who align philosophically with the pedagogical training delivered by university programs (Korthagen & Kessels, 1999; Tom, 1997), specifically, cooperating teachers who can reinforce the theoretical framework underpinning the professional coursework pre-service teachers experience in university teacher preparation programs. This qualitative study sought to better understand the feedback provided to future school-based agricultural education (SBAE) teachers during their student teaching experience. Through initial and secondary coding, the research team identified themes among the feedback provided to student teachers by their cooperating teachers. The study revealed cooperating teacher feedback reflects the pedagogical training provided via the teacher preparation program, specifically, around effective teaching behaviors. Understanding the cooperating teacher feedback provides insight for teacher preparation programs

    The influence of shc proteins and aging on whole body energy expenditure and substrate utilization in mice.

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    While it has been proposed that Shc family of adaptor proteins may influence aging by regulating insulin signaling and energy metabolism, the overall impact of Shc proteins on whole body energy metabolism has yet to be elucidated. Thus, the purpose of this study was to determine the influence of Shc proteins and aging on whole body energy metabolism in a mouse model under ambient conditions (22°C) and acute cold exposure (12°C for 24 hours). Using indirect respiration calorimetry, we investigated the impact of Shc proteins and aging on EE and substrate utilization (RQ) in p66 Shc-/- (ShcKO) and wild-type (WT) mice. Calorimetry measurements were completed in 3, 15, and 27 mo mice at 22°C and 12°C. At both temperatures and when analyzed across all age groups, ShcKO mice demonstrated lower 24 h total EE values than that of WT mice when EE data was expressed as either kJ per mouse, or adjusted by body weight or crude organ mass (ORGAN) (P≤0.01 for all). The ShcKO mice also had higher (P<0.05) fed state RQ values than WT animals at 22°C, consistent with an increase in glucose utilization. However, Shc proteins did not influence age-related changes in energy expenditure or RQ. Age had a significant impact on EE at 22°C, regardless of how EE data was expressed (P<0.05), demonstrating a pattern of increase in EE from age 3 to 15 mo, followed by a decrease in EE at 27 mo. These results indicate a decline in whole body EE with advanced age in mice, independent of changes in body weight (BW) or fat free mass (FFM). The results of this study indicate that both Shc proteins and aging should be considered as factors that influence energy expenditure in mice

    The influence of shc proteins on the whole body energetic response to calorie restriction initiated in 3-month-old mice.

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    There is increasing evidence that Shc proteins play a role in energy metabolism, and we have previously reported that knockdown of Shc proteins influences the energetic response to acute (3 days) calorie restriction (CR) in 18-month-old mice. Whether Shc proteins play a role in the metabolic response to CR in younger mice has yet to be elucidated. Hence, we sought to determine the impact of 3 days and longer term (2 months) CR on energy expenditure (EE) and respiratory quotient (RQ) in 3 month-old Shc knockout (ShcKO) and wild-type (WT) mice. ShcKO mice decreased (P < 0.001) EE normalized for body weight (EEBW) by 3 days of CR, while no such change was observed in WT animals. However, both ShcKO and WT mice decreased (P < 0.001) EEBW at 2 months of CR and there were no differences in body weight between the ShcKO and WT mice at either 3 days or 2 months of CR. Consistent with increased fatty acid oxidation, only ShcKO mice maintained decreased (P < 0.001) 24 h RQ through 2 months of CR, suggesting that they were able to maintain increased fatty acid oxidation for a longer period of time than WT mice. These results indicate that Shc proteins may contribute to some of the acute energetic responses to CR

    Barriers to Conducting Supervised Agricultural Experiences as Perceived by Preservice Education Teachers

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    The purpose of this descriptive study was to assess preservice agriculture teachers’ perceptions of the importance of Supervised Agricultural Experience (SAE) and their views on barriers to conducting SAE. A census of the sophomore-level agricultural education course at Oklahoma State University was conducted to measure perceptions at the beginning and end of the course. This study was framed upon Ajzen’s Theory of Planned Behavior. Results indicated that preservice teachers perceived SAE was an important component of agricultural education and important at the secondary school they attended. The greatest barrier to conducting SAE was their lack of familiarity with newer SAE categories. This was true at both the beginning and end of the course. It is recommended that preservice teachers receive instruction on and experiences in all types of SAE. This would increase the likelihood of preservice teachers perceiving they have control over this barrier regarding SAE implementation. This cohort of preservice teachers should be surveyed over time to determine change in their perceptions of barriers to SAE implementation as they progress in the agricultural education program and through their careers. Further, the views of in-service teachers should also be assessed to determine if perceived barriers differ with professional experience

    Kepler Mission Stellar and Instrument Noise Properties Revisited

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    An earlier study of the Kepler Mission noise properties on time scales of primary relevance to detection of exoplanet transits found that higher than expected noise followed to a large extent from the stars, rather than instrument or data analysis performance. The earlier study over the first six quarters of Kepler data is extended to the full four years ultimately comprising the mission. Efforts to improve the pipeline data analysis have been successful in reducing noise levels modestly as evidenced by smaller values derived from the current data products. The new analyses of noise properties on transit time scales show significant changes in the component attributed to instrument and data analysis, with essentially no change in the inferred stellar noise. We also extend the analyses to time scales of several days, instead of several hours to better sample stellar noise that follows from magnetic activity. On the longer time scale there is a shift in stellar noise for solar-type stars to smaller values in comparison to solar values.Comment: 10 pages, 8 figures, accepted by A

    Agricultural Education Teacher Candidates’ Reflection on the 15-Week Clinical Teaching Internship: A Collective Case Study

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    This collective case study explored the integration of video reflection during the 15-week clinical teaching internship at three distinct points in time. Weeks three, seven, and 11 were investigated to determine if the integration of video reflection improved the quality of teacher candidate reflection and to gauge teacher candidates’ views on agricultural education during the clinical teaching internship. Four overarching themes emerged from the video reflections: establishing roles as a teacher, teaching and learning, balance, and coming full circle. Throughout the internship, candidates expressed a deeper understanding of teaching within the three-component model of school-based agricultural education (SBAE). We recommend the use of video reflection during the student teaching internship to expand the depth of reflection and feeling of community among the cohort. Overall, the addition of the video reflection process has provided an efficient and effective tool that allows teacher candidates to make meaning of their experience

    Mice with low levels of Shc proteins display reduced glycolytic and increased gluconeogenic activities in liver.

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    Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins

    Deep Search For Molecular Oxygen in TW Hya

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    The dominant form of oxygen in cold molecular clouds is gas-phase carbon monoxide (CO) and ice-phase water (H2_2O). Yet, in planet-forming disks around young stars, gas-phase CO and H2_2O are less abundant relative to their ISM values, and no other major oxygen-carrying molecules have been detected. Some astrochemical models predict that gas-phase molecular oxygen (O2_2) should be a major carrier of volatile oxygen in disks. We report a deep search for emission from the isotopologue 16^{16}O18^{18}O (NJ=2101N_J=2_1-0_1 line at 233.946 GHz) in the nearby protoplanetary disk around TW Hya. We used imaging techniques and matched filtering to search for weak emission but do not detect 16^{16}O18^{18}O. Based on our results, we calculate upper limits on the gas-phase O2_2 abundance in TW Hya of (6.470)×107(6.4-70)\times10^{-7} relative to H, which is 232-3 orders of magnitude below solar oxygen abundance. We conclude that gas-phase O2_2 is not a major oxygen-carrier in TW Hya. Two other potential oxygen-carrying molecules, SO and SO2_2, were covered in our observations, which we also do not detect. Additionally, we report a serendipitous detection of the C15^{15}N NJ=25/213/2N_J = 2_{5/2}-1_{3/2} hyperfine transitions, F=32F = 3 - 2 and F=21F = 2 - 1, at 219.9 GHz, which we found via matched filtering and confirm through imaging.Comment: 10 pages, 6 figures, Accepted for publication in Ap

    The influence of dietary lipid composition on skeletal muscle mitochondria from mice following eight months of calorie restriction

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    PMCID: PMC4138957.-- et al.Calorie restriction (CR) has been shown to decrease reactive oxygen species (ROS) production and retard aging in a variety of species. It has been proposed that alterations in membrane saturation are central to these actions of CR. As a step towards testing this theory, mice were assigned to 4 dietary groups (control and 3 CR groups) and fed AIN-93G diets at 95 % (control) or 60 % (CR) of ad libitum for 8 months. To manipulate membrane composition, the primary dietary fats for the CR groups were soybean oil (also used in the control diet), fish oil or lard. Skeletal muscle mitochondrial lipid composition, proton leak, and H2O2 production were measured. Phospholipid fatty acid composition in CR mice was altered in a manner that reflected the n-3 and n-6 fatty acid profiles of their respective dietary lipid sources. Dietary lipid composition did not alter proton leak kinetics between the CR groups. However, the capacity of mitochondrial complex III to produce ROS was decreased in the CR lard compared to the other CR groups. The results of this study indicate that dietary lipid composition can influence ROS production in muscle mitochondria of CR mice. It remains to be determined if lard or other dietary oils can maximize the CRinduced decreases in ROS production. © 2014 Institute of Physiology v.v.i.This work was supported by the National Institutes of Health grants R01 AG028125 and P01 AG025532.Peer Reviewe
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