Pneumonia Outbreak in Bighorn Sheep in the East Fork of the Bitterroot: A Summary of Pathology and Laboratory Findings

A bighorn sheep (Ovis Canadensis) pneumonia outbreak began in the East Fork of the Bitterroot River drainage in late November 2009. The decision to cull apparently sick animals provided the unique opportunity to collect fresh, high quality biological samples for diagnostic testing. It is our hope that information gained from this outbreak will contribute to an understanding of bighorn sheep pneumonia outbreaks in western states. The Montana Fish, Wildlife, and Parks wildlife laboratory performed full necropsies on many of the bighorn sheep that died or were culled during this outbreak. Body condition score and severity of lung lesions was noted. A fresh blood sample and fecal sample was collected, and the pharynx and ear canal were swabbed. Lung, tracheobronchial lymph node, and liver samples were also collected. Field personnel collected fresh tissue samples from culled sheep that could not be removed from the field for necropsy. Tissues and swabs were submitted to Washington Animal Disease Diagnostic Laboratory (WADDL) for aerobic and Mycoplasma culture. Serum and fecal samples were submitted to the Montana Department of Livestock laboratory in Bozeman. In this presentation we will describe the gross pathology of the pneumonia outbreak, summarize all laboratory findings, and describe similarities and differences when compared to other bighorn sheep pneumonia outbreaks within Montana and in other western states

Culling as an Exploratory Field Technique to Reduce Overall Mortality During a Pasturella Spp. Outbreak in a Montana Bighorn Sheep Population

Several herds of Rocky Mountain bighorn sheep (Ovis Canadensis) in the United States and Canada have experienced all-age die-offs during outbreaks of Pasturella spp. Induced pneumonia. Isolating triggers and remedies for these die-offs remains elusive. Montana Fish, Wildlife and Parks used the statewide Draft Sheep Conservation Strategy as a guide in establishing a field culling-mobile laboratory-media response to a pneumonia/complex outbreak in the East Fork Bitterroot bighorn sheep herd. Montana Fish, Wildlife and Parks employees along with volunteers from the Ravalli County Fish and Wildlife Association, Wild Sheep Foundation and the USDA Forest Service culled 76 sheep from a herd numbering at least 187 animals according to spring 2009 aerial observations. Field personnel discovered six recent bighorn sheep carcasses when culling efforts began in late November. Field personnel discovered one additional bighorn carcass during the three-month culling process. Lab experts conducting onsite necropsies observed evidence of infection in 73 (96%) of the culled sheep. State biologists observed 93 bighorns on this winter range during a cursory aerial survey conducted on 28 December 2009. Preliminary observations from comparing results of sheep selected for culling to field necropsies suggest field personnel detect infected sheep with a high degree of accuracy. We suggest that this technique prevented additional mortalities directly related to pneumonia

Novel muon imaging techniques

Owing to the high penetrating power of high-energy cosmic ray muons, muon imaging techniques can be used to image large bulky objects, especially objects with heavy shielding. Muon imaging systems work just like CT scanners in the medical imaging field—that is, they can reveal information inside of a target. There are two forms of muon imaging techniques: muon absorption imaging and muon multiple scattering imaging. The former is based on the flux attenuation of muons, and the latter is based on the multiple scattering of muons in matter. The muon absorption imaging technique is capable of imaging very large objects such as volcanoes and large buildings, and also smaller objects like spent fuel casks; the muon multiple scattering imaging technique is best suited to inspect smaller objects such as nuclear waste containers. Muon imaging techniques can be applied in a broad variety of fields, i.e. from measuring the magma thickness of volcanoes to searching for secret cavities in pyramids, and from monitoring the borders of countries checking for special nuclear materials to monitoring the spent fuel casks for nuclear safeguards applications. In this paper, the principles of muon imaging are reviewed. Image reconstruction algorithms such as Filtered Back Projection and Maximum Likelihood Expectation Maximization are discussed. The capability of muon imaging techniques is demonstrated through a Geant4 simulation study for imaging a nuclear spent fuel cask

Small business and information systems

We are pleased to present this AJIS featured theme on Small Business and Information Systems, which is the result of a merger between AJIS and the Journal of Information Systems and Small Business. The co-editors of both journals felt that the merger would help raise the profile of small business research in Australia (since AJIS is more highly recognised than JISSB) and that it would increase the number of publications in AJIS. It is also a global featured theme, with papers from Australia, New Zealand, the United Kingdom and Ireland

Outcomes of Treated Human Granulocytic Ehrlichiosis Cases

We conducted a case-control study in Wisconsin to determine whether some patients have long-term adverse health outcomes after antibiotic treatment for human granulocytic ehrlichiosis (HGE). A standardized health status questionnaire was administered to patients and controls matched by age group and sex. Consenting patients provided blood samples for serologic testing. Among the 85 previously treated patients, the median interval since onset of illness was 24 months. Compared with 102 controls, patients were more likely to report recurrent or continuous fevers, chills, fatigue, and sweats. Patients had lower health status scores than controls for bodily pain and health relative to 1 year earlier, but there was no significant difference in physical functioning, role limitations, general health, or vitality measures. The HGE antibody titer remained elevated in one patient; two had elevated aspartate aminotransferase levels. HGE may cause a postinfectious syndrome characterized by constitutional symptoms without functional disability or serologic evidence of persistent infection

evoText: A new tool for analyzing the biological sciences

We introduce here evoText, a new tool for automated analysis of the literature in the biological sciences. evoText contains a database of hundreds of thousands of journal articles and an array of analysis tools for generating quantitative data on the nature and history of life science, especially ecology and evolutionary biology. This article describes the features of evoText, presents a variety of examples of the kinds of analyses that evoText can run, and offers a brief tutorial describing how to use it

Preliminary Findings of an Elk Brucellosis Surveillance and Epidemiology Project in Southwestern Montana

Brucellosis is a bacterial disease that causes abortions in cattle, bison (Bison bison) and elk (Cervus elaphus). Transmission of the disease from wildlife to cattle has serious financial implications to producers and the livestock industry in Montana. Brucellosis in elk populations of southwestern Montana results in reduced tolerance for elk on private property and can influence management of elk populations. In the winter of 2010/2011, Montana Fish, Wildlife and Parks initiated a five-year project with the goals of delineating the geographical distribution of brucellosis in elk populations, enhancing our understanding of how brucellosis functions in elk populations, and evaluating factors that may influence the spread and prevalence of brucellosis in elk. One-hundred adult female elk were captured in hunting districts (HD) 324 and 326 in the winter of 2010/2011 with eight testing positive on blood tests(seropositive) in the field for exposure to Brucella. Ninety-three adult female elk were captured in HD 325 in the winter of 2011/2012, five of which were seropositive. Elk testing positive in the field were fitted with a GPS collar and, if pregnant, implanted with a vaginal implant transmitter (VIT). Seropositive pregnant elk were tracked from the ground and air 2-3 times/week in order to locate birth or abortion sites. B. abortus was not cultured from VITs or samples collected at birth sites in the first year of the project. B. abortus was cultured from tissues or VITs associated with two aborted calves in 2012. The known distribution of brucellosis in elk has expanded based on information obtained in this study

Elk Movements and Harvest Across Public and Private Lands in the Sapphire Mountains

Hunting access issues have become increasingly contentious as changes in land ownership and use have influenced elk distributions in some areas. In the Sapphire Mountains of western Montana, hunters have voiced concerns regarding elk aggregations on privately-owned lands that restrict hunter access.   To address these concerns, we initiated a survey of landowners and hunters to determine satisfaction with elk management.  We also radiocollared 65 elk (45 cows, 20 bulls) to better understand elk distributions across public and private lands.  In all seasons, bull elk locations were more likely to occur in publicly accessible areas than cow elk locations. During archery season, 61% of bull locations and 41% of cow locations occurred in publicly accessible areas.  These numbers dropped to 48% of bull locations and 14% of cow locations in publicly accessible areas during rifle season.  During archery and rifle seasons combined, 1 of 39 radiocollared cows (2.4%) was harvested on private land, and 5 of 19 radiocollared bulls (26.3%) were harvested: 3 on publicly accessible land and 2 on private land. Although hunters reported a lack of elk on public lands as a concern, our radiocollar and harvest data confirm that at least a segment of the bull population was accessible to public hunters, but female elk were aggregated in areas that restricted hunter access.  A lack of hunter access to female elk during the hunting season may result in management challenges, including game damage issues, and increases in the population beyond objective levels

Nutritional and Demographic Consequences of Varying Elk Migratory Behaviors

Elk (Cervus elaphus) populations in the American West exhibit wide variation in migratory behavior. The traditional view of elk migration holds that migratory elk move from winter range in order to track growth of highly nutritious fresh vegetation into higher elevation areas. Non-migratory elk forego this seasonal movement, typically foraging in lower elevation winter range areas throughout the summer. Although the effect of summer nutrition on elk body condition and reproductive success is well known, the nutritional and demographic consequences of these differing migratory behaviors remain unclear. We developed a predictive model of summer forage quality to compare the nutrition available to migrants and non-migrants in a partially migratory population of elk in western Montana. Non-migratory elk had access to significantly higher forage quality than their migratory counterparts; the lower forage quality available to migrants is predicted to result in reduced reproductive success based on published studies linking nutrition with elk demographic rates. We therefore expect non-migrants to have higher fecundity rates and to comprise a higher proportion of the population relative to migrants. Harvest management actions that reduce survival rates of non-migrants or increase survival rates of migrants may be an effective tool for maintaining migratory behavior in partially migratory populations

Use of Foliar Chemical Treatments to Induce Disease Resistance in Rhododendrons Inoculated with Phytophthora ramorum

A field study was conducted at the National Ornamental Research Site at Dominican University California (NORS-DUC). The study goal was to evaluate three chemical inducers applied as foliar treatments for controlling Phytophthora ramorum, on Rhododendron x ‘Cunningham’s White’ nursery plants. The inducers were chlorine dioxide (ElectroBiocide), hydrogen peroxide (OxiDate 2.0), and acibenzolar-s methyl (Actigard). Water samples from the electrostatic sprayer were measured for three physicochemical water properties. Visual assessment of plant foliage, based on the Horsfall- Barratt scale, was conducted at three and five months after chemical treatments. Foliar fluorescence (Fv/Fm) was measured over three dates. The success of P. ramorum inoculations were determined using qPCR methods. Visual assessment across both months showed no signs of P. ramorum infection or chemical injury symptoms. However, P. ramorum infection vis-à-vis qPCR analysis was confirmed. The September Fv/Fm results revealed that all the chemical inducer treatments were equivalent to the water treatment, except for Actigard. The qPCR results were in general agreement with the Fv/Fm results indicating that the rhododendrons were successfully inoculated with P. ramorum but were non-symptomatic. The electrostatic sprayer ionized the water droplets, resulting in increased Fv/Fm values for the water treatments 90 days after application. There was a three-month delay in fluorescence responses to the most effective chemical applications, indicating that woody plants may need to be monitored over the long term to determine accurate responses to foliar treatments