A simple and rapid method for isolation of cellular DNA

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Evaluation of Bacteriological Water Quality, Bangalore-In View of Public health.

From a public health perspective, access to sufficient amounts of clean and safe drinking water is a crucial issue. The provision of good quality household drinking water is often regarded as an important means of improving health. The study was undertaken to analyze the bacterial water quality in Bangalore district, Karnataka. The bacteriological analysis was carried out using the multiple tube technique (MPN- Most Probable Number) for detection of faecal coliform and subsequently organisms present in the sample of water were identified following standard methods. The identified organisms include E. coli, Staphylococcus species, Citrobacter species, Enterobacter, Klebsiella pneumonia, Proteus species, Pseudomonas, Serratia species. 60% of the isolated organism being of the family Enterobacteriaceae. The total heterotrophic plate count (THPC) gave a range of 1.5*104 to 2.2*104 CFU/ml while the total coliform plate count (TCPC) gave a range of 2*103 to 8.3*103 CFU/ml. The presumptive faecal coliform ranged between 0-180 coliform per 100 ml. E. coli / fecal coliform were detected in 60 % of the water sample

Regulatory Model of Mirna-125 in Leukemia

MicroRNAs (miRNAs) are small, subtypes of RNA that are 22-25 nucleotides in length that regulate the gene expression by targeting homologous sequences in messenger RNA (mRNA). Their unusual expressions have been observed in various types of cancers. In this paper we first briefly outline of multiple pairwise alignment of primiRNA- 125 of three organisms and secondary structure. We then discuss in detail on binding sites of miRNA-125 with different target genes to known the binding energy using computational tool. Finally, we discuss on mechanism of miRNA-125 over-expression causes different types of leukemia due to abnormal expression of gene present on chromosome 21. Thus we have reported that one of the major causes of leukemia is abnormal expression of miRNA- 125. Ful

Water quality studies of Hoskerehalli lake of Bangalore, Karnataka

The aim of the current study was to evaluate the status of the Hoskerehalli lake water with respect to different physicochemical parameters [pH, temperature, total dissolved solids (TDS), turbidity, electrical conductivity (EC), total hardness (TH), alkalinity, dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), phosphate, sulphate, nitrate and potassium] during the year 2005. These parameters were compared with water quality standards to indicate probable pollution. However, the overall water quality of the lake remained within the safe limits throughout the study period. Correlation coefficient matrix between each parameter were estimated to throw light on relationship between different physicochemical parameters under investigation, few parameters like pH - temperature (r = 0.712), TDS - turbidity (r = 0.800), TDS - conductivity (r = 0.988), turbidity - conductivity (r = 0.781), temperature - alkalinity (r = 0.822), sulphate - potassium (r = 0.724) showed good positive correlation. The lake water quality is good and fit for irrigation and fish culture purpose

Computational prediction and identification of miRNAs in ssRNA viruses

MiRNAs are conserved endogenous small non-​coding RNA mols. play a very important role in posttranscriptional regulation of gene by binding to mRNA.miRNAs have been identified in plants, animals, invertebrates and even in viruses. Recent studies have proved that as like DNA viruses even cytoplasmic RNA viruses encodes miRNA by noncanonical pathway.To find the miRNAs encoded by both (+) ssRNA and (-​) ssRNA viruses, we used computational tool VMir to screen the complete genomes of selected RNA viruses. The predicted precursor miRNA hairpin structures obtained from VMir were further analyzed in miPred to identify real and pseudo miRNA. The real miRNAs analyzed in blastx program to exclude protein coding sequences and then finally identified mature miRNAs by using matureBayes.In our studies we analyzed 45 viral genomes, finally we obsd. that more miRNAs encoded by (-​) ssRNA than the (+) ssRNA

Evaluation of the Microbial Pollution of Water in Ramasandra Lake, Bangalore, Karnataka and Assessment of Multiple Antibiotic Resistance among Escherichia Coli

An experiment was designed to examine microbiology of water samples of Ramasandra Lake and further assess the occurance of the multiple antibiotic resistance of E coli. Analysis of the water samples obtained from the lake indicates high microbial and fecal contamination with microbial load in order of 105 and MPN >1800/100ml. Collected water samples from four stations were screened for the E coli to assess their resistance to 10 different antibiotics. Of the 14 E coli isolates 0% were susceptible to all antibiotics used. The isolates were found resistance to penicillin and tetracycline (100%). Among the 10 antibiotics tested, four pattern of antibiotics resistance were obtained and all of them were multiple antibiotic resistance with the number of antibiotics ranging from 2 to 7. The result indicates the developing antibiotic resistant E coli may be a serious threat on public health, aquatic organisms and environment

Computational identification and characterization of putative miRNAs in Nasonia species

MicroRNAs are important at post transcriptional regulation in eukaryotes. Nasonia genus is becoming increasingly popular model in present days due to genetic advantages it possesses over Drosophila. Nasonia species are found distributed throughout the world, expect for N. longicornis, and N. giraulti. In this study, we use the sequential method of blasting all known invertebrate miRNA genes against the Nasonia vitripennis, Nasonia longicornis, and Nasonia giraulti genomes. We identify 40, 31 and 29 putative pre-​miRNAs and mature sequences in N. vitripennis, N. giraulti and N. longicornis resp. A cross species comparison of putative miRNA sequences and their statistical characteristics reveals that there are no huge differences between the species, except for few miRNAs which are reported. We also find that the minimal folding energy index for three Nasonia species pre-​miRNA's av. is around -​0.85 ± 0.11. Further, we report that U is predominant at the 5' end of mature sequence, which being a typical characteristic of plant miRNAs. Using MiRanda, we predict nearly 471 potential sites in the N. vitripennis genome. Thus concluding our study to be the beginning of understanding the Nasonia's non coding RNAs and may play an important role in effective pest management in near future

Radiation and mass transfer effects on an unsteady MHD free convection flow past a heated vertical plate in a porous medium with viscous dissipation

An unsteady, two-dimensional, hydromagnetic, laminar free convective boundary-layer flow of an incompressible, Newtonian, electrically-conducting and radiating fluid past an infinite heated vertical porous plate with heat and mass transfer is analyzed, by taking into account the effect of viscous dissipation. The dimensionless governing equations for this investigation are solved analytically using two-term harmonic and non-harmonic functions. Numerical evaluation of the analytical results is performed and graphical results for velocity, temperature and concentration profiles within the boundary layer and tabulated results for the skin-friction coefficient, Nusselt number and Sherwood number are presented and discussed. It is observed that, when the radiation parameter increases, the velocity and temperature decrease in the boundary layer, whereas when thermal and solutal Grashof increases the velocity increases

Efficacy of bipolar hemiarthroplasty in the elderly people: a study in a tertiary care centre

Background: Intertrochanteric fractures are associated with severe displacement and comminution are very common among the elderly patients especially women. Hemiarthroplasty is a procedure, where the head of the femur is replaced with a prosthetic implant. This study was performed to evaluate the efficacy of the bipolar hemiarthroplasty among the elderly patients with intra capsular fractures.Methods: Bipolar hemiarthroplasty with or without cement was performed on 77 patients above the age of 60 years with femoral neck fractures.Results: Out of the 77 patients, 79.2% were females and 20.8% were males. The mean age of all the patients was 68.4 years. Type IV was found to be most common among the elderly. The outcome of the surgery was excellent in more than 75% of the cases. Only one patient had a poor outcome. The most common complication of the surgery was limb shortening in 5.2% patients followed by superficial infections in 2.6%. Conclusions: Bipolar hemiarthroplasty for the unstable hip fractures in the elderly has good results with early mobilizations and minimal complications. This ultimately affects the general health of the patients and reduces morbidity and mortality